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The map label for this gene is tenA [H]

Identifier: 159041063

GI number: 159041063

Start: 518801

End: 519463

Strand: Reverse

Name: tenA [H]

Synonym: Cmaq_0480

Alternate gene names: 159041063

Gene position: 519463-518801 (Counterclockwise)

Preceding gene: 159041066

Following gene: 159041062

Centisome position: 25.0

GC content: 43.74

Gene sequence:

>663_bases
ATGACTAAGTTAAGTGAAGTCCTCTGGTCAAGTATTAGGGATGTCTATGACGCAATAATTAAGCACCCATTCATTAGGGG
TTTAGCTGATGGTTCATTAAGTGAGGATATGTTCAAGTACTACATTATCCAAGACCACATGTATCTTGGGGAGTACATTA
AGGCGTTGTCAATAATAGCCGCCAAGGCACCTAGGATTGAGGAGGCTCAATTATTCCTAAGACACTCAATAGGTGCCATT
GAGGTTGAGAGGAGGCTTCATGAACACTACATGAGGATATGGAACCTTAACCCAGCGGAGTACGTAATGTCACCCACTAA
TAGGGCGTACACAAGCTTCCTCATAACTGAAGCCTACTCTAAACCCTACTACGAGGCCATTGCGGCAGTGTTACCATGCT
ACTGGATTTACGAGAAGGTTGGGGTTGAGTTATCTAAGCATGAGGTTAGTAATGAGAATTACAGGCTATGGGTCTCAACG
TACAGTGGGGAGGAGTATAGGGCATCCGTTAGGGAGGTATTGAGTATAGTAGACTCAATGAACGTTACTGAGAGTCAATT
AAATGACATGATTAAGGCATTCAGACTAGCCTCAATATACGAGTACATGTTCTGGGACAGTGCCTACAGGATGGAGAAGT
GGCCAATAAACCCAAACCCCTAA

Upstream 100 bases:

>100_bases
AAAGCCTAAAATTAATGATACTTTAATTAATGCATAACTAACCCCTTAAGTACGCGGCCCAGCAATAACACTTTTATAGG
CAACCTAATACTTCCTAATA

Downstream 100 bases:

>100_bases
TCAATTAGGACGTGAAACAATGGAACCAAGACCTTTAAAATCAACATGAATGTGAATTAATGTTAATTTACGCATTATCT
TACATTGCCTTAACTCTTAA

Product: TenA family transcription regulator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTKLSEVLWSSIRDVYDAIIKHPFIRGLADGSLSEDMFKYYIIQDHMYLGEYIKALSIIAAKAPRIEEAQLFLRHSIGAI
EVERRLHEHYMRIWNLNPAEYVMSPTNRAYTSFLITEAYSKPYYEAIAAVLPCYWIYEKVGVELSKHEVSNENYRLWVST
YSGEEYRASVREVLSIVDSMNVTESQLNDMIKAFRLASIYEYMFWDSAYRMEKWPINPNP

Sequences:

>Translated_220_residues
MTKLSEVLWSSIRDVYDAIIKHPFIRGLADGSLSEDMFKYYIIQDHMYLGEYIKALSIIAAKAPRIEEAQLFLRHSIGAI
EVERRLHEHYMRIWNLNPAEYVMSPTNRAYTSFLITEAYSKPYYEAIAAVLPCYWIYEKVGVELSKHEVSNENYRLWVST
YSGEEYRASVREVLSIVDSMNVTESQLNDMIKAFRLASIYEYMFWDSAYRMEKWPINPNP
>Mature_219_residues
TKLSEVLWSSIRDVYDAIIKHPFIRGLADGSLSEDMFKYYIIQDHMYLGEYIKALSIIAAKAPRIEEAQLFLRHSIGAIE
VERRLHEHYMRIWNLNPAEYVMSPTNRAYTSFLITEAYSKPYYEAIAAVLPCYWIYEKVGVELSKHEVSNENYRLWVSTY
SGEEYRASVREVLSIVDSMNVTESQLNDMIKAFRLASIYEYMFWDSAYRMEKWPINPNP

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325378, Length=210, Percent_Identity=25.2380952380952, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324997, Length=215, Percent_Identity=26.046511627907, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25901; Mature: 25770

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSEVLWSSIRDVYDAIIKHPFIRGLADGSLSEDMFKYYIIQDHMYLGEYIKALSIIA
CCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAPRIEEAQLFLRHSIGAIEVERRLHEHYMRIWNLNPAEYVMSPTNRAYTSFLITEAYS
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHC
KPYYEAIAAVLPCYWIYEKVGVELSKHEVSNENYRLWVSTYSGEEYRASVREVLSIVDSM
CHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCC
NVTESQLNDMIKAFRLASIYEYMFWDSAYRMEKWPINPNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKLSEVLWSSIRDVYDAIIKHPFIRGLADGSLSEDMFKYYIIQDHMYLGEYIKALSIIA
CHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAPRIEEAQLFLRHSIGAIEVERRLHEHYMRIWNLNPAEYVMSPTNRAYTSFLITEAYS
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHC
KPYYEAIAAVLPCYWIYEKVGVELSKHEVSNENYRLWVSTYSGEEYRASVREVLSIVDSM
CHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCC
NVTESQLNDMIKAFRLASIYEYMFWDSAYRMEKWPINPNP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]