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The map label for this gene is topA [H]

Identifier: 159040701

GI number: 159040701

Start: 133817

End: 135649

Strand: Reverse

Name: topA [H]

Synonym: Cmaq_0109

Alternate gene names: 159040701

Gene position: 135649-133817 (Counterclockwise)

Preceding gene: 159040704

Following gene: 159040699

Centisome position: 6.53

GC content: 46.21

Gene sequence:

>1833_bases
ATGGATAAGTTAATTGTTGCTGAAAAGAACAGTGTGGCTAAGGCTATAGCCCAATACTTAGCTGAGGGTGGATACACATT
AAGGAGAATTGGTATTGTACCCGTCTACTTCTTTAAGGTTAATGGGGAGTATTGGGCATCCATGGGCCTAAGGGGGCATA
TCCTTGACTTCGACTTTGAACACTCCTATAATAATTGGAACAGAGTGGAGCCGGGTAAGCTCCTTGACCTTGAGCCAGTA
ATGGTGATTAGGGGTTGGGATAGGCCGTACGTAACGGCGTTGGTTGAATTATCGAAGCAGGCTAGGGAAATTATCCTCGC
CCTAGACTCTGATGTTGAGGGTGAGGCAATAGCCTACGAGGTAATGCTTGTGACTAGGCTTAGGAAACCCACCTTAAGGT
TTAGGAGGGCATTATTCTCAGCGGTCACTAGGGATGATATTAGGAGGGCATTCAGTAAGTTAACAACAATCAACGTTAAC
CTTGCTAGGAAGGTCTTCACCAGAATGGTTATTGACCTTAAGTACGGTGCAACATTCACTAGGCTATTAACCTTAAGCGC
CAAGTCAAGTAAGGCGCCATTAAATAGGGGTGAGTTCCTAAGCTACGGCCCCTGTCAAACACCGGTGCTTAACCTAGTTG
TTCAAAGAGCCTTGGAGAGGGAGAATTTTAAGCCTGAGGTTTACTATAAGATTAAATTAATCATTGAGGCTAACGGTGAG
TTAATTGAATTAGAGTCCATTGATAAGTTTAAGAACCTTAAGGAGGCCCAGGAGGCGCTTAGTAGGGTTAAGGCAGGTAA
GGCGGTGGTTAAGAATATTGAGGCTAAGAGGGTTCACGTTAATCCACCTAAGCCCCTTGAAACCGTGGAGCTTGAGAGGA
GGGCTAGTAGATTCCTCAACATTAGGAGTAAGCAGACCCTTGATGCAGCAGAGGAGCTTTACAGGCAGGGTTACATATCC
TACCCAAGAACTGAAACCAACATTTACCCACCCACCCTGGATTTAAGGGGTATTTTAAGGAACCTAACCTCAACGTCAAC
CTACGGTCAATACGCAAGGCATCTACTGGCTGGTGAATTAAGGCCTACTGCAGGTAAGGATAATGATAACGCCCACCCGC
CAATACACCCGGTTAAGGCTGCTGATAAGCCTGAGTTAATGGCTAGGTTCAGGGACTTTAAGTACTGGCTCATATACGAC
CTGGTGGTTAGGCATTTCCTAGCAACCCTAAGTCCCCCAGCCTTAATTGAGGAGCAGAGGCTTACTGTTGATGCTGGTGG
AGTACTCTTCGAGGCTTCAGGCCTCAGGATCATTAATGACGGCTACTTCACGATTTACCCCTTCGAGAGACCTAGGGCTA
ATCCACTACCCTTAAGCGCATTAAGGATTGGTATGCAGGTCACTGTTAAGGATGCTAAGGTTGTTAAGAGGAAGACCACG
CCGCCACCCTACTTAAGTGAATCAGAGTTGCTTAGGTTAATGAGGAAGTACGGTATAGGTACAGACGCCACTATGCAGGA
CCACATACATACTAACGTTAAGAGAAGGTACTTCAAGATAATTAAGGGGCAGTGTGTACCCACACCGCTTGGTAAAGCGT
TAATAACCTCATTATCCAAGTACGCACCAACGTTAATAGACCCAAACTTCAGGAGTAGGATGGAGTCCATGCTTTCACTC
ATTGGTTCAGGGAAGGAGATGCCTGACTCAGTGAGGAGGAGGCTTGAGGAGGAGGCCGCTAGGGTTTACACCTCAATGAA
GCCTAATTCTAATCAACTCGGGGAAGAATTAGCTAAGGCGTTGAGAAGCATGGTTAATGAAAAGGGCGCTTAA

Upstream 100 bases:

>100_bases
AGGCGTCTGCTTCTTCTCATCAGGCTTCTTCTCACTTGACATCTCAATCAACCTAAATTTAGTGAAGGATTTATTAAATG
TTTCGCTTGAATACTGTTGA

Downstream 100 bases:

>100_bases
TCACCATACCTACCCTCCACCTTTCTCGGGCGTCTAATAAAGACCCTATAGGCACCCACTGAGGCCAGGGGTATTGTGAA
TAATACAAGGAAGGCGTACG

Product: DNA topoisomerase type IA central domain-containing protein

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MDKLIVAEKNSVAKAIAQYLAEGGYTLRRIGIVPVYFFKVNGEYWASMGLRGHILDFDFEHSYNNWNRVEPGKLLDLEPV
MVIRGWDRPYVTALVELSKQAREIILALDSDVEGEAIAYEVMLVTRLRKPTLRFRRALFSAVTRDDIRRAFSKLTTINVN
LARKVFTRMVIDLKYGATFTRLLTLSAKSSKAPLNRGEFLSYGPCQTPVLNLVVQRALERENFKPEVYYKIKLIIEANGE
LIELESIDKFKNLKEAQEALSRVKAGKAVVKNIEAKRVHVNPPKPLETVELERRASRFLNIRSKQTLDAAEELYRQGYIS
YPRTETNIYPPTLDLRGILRNLTSTSTYGQYARHLLAGELRPTAGKDNDNAHPPIHPVKAADKPELMARFRDFKYWLIYD
LVVRHFLATLSPPALIEEQRLTVDAGGVLFEASGLRIINDGYFTIYPFERPRANPLPLSALRIGMQVTVKDAKVVKRKTT
PPPYLSESELLRLMRKYGIGTDATMQDHIHTNVKRRYFKIIKGQCVPTPLGKALITSLSKYAPTLIDPNFRSRMESMLSL
IGSGKEMPDSVRRRLEEEAARVYTSMKPNSNQLGEELAKALRSMVNEKGA

Sequences:

>Translated_610_residues
MDKLIVAEKNSVAKAIAQYLAEGGYTLRRIGIVPVYFFKVNGEYWASMGLRGHILDFDFEHSYNNWNRVEPGKLLDLEPV
MVIRGWDRPYVTALVELSKQAREIILALDSDVEGEAIAYEVMLVTRLRKPTLRFRRALFSAVTRDDIRRAFSKLTTINVN
LARKVFTRMVIDLKYGATFTRLLTLSAKSSKAPLNRGEFLSYGPCQTPVLNLVVQRALERENFKPEVYYKIKLIIEANGE
LIELESIDKFKNLKEAQEALSRVKAGKAVVKNIEAKRVHVNPPKPLETVELERRASRFLNIRSKQTLDAAEELYRQGYIS
YPRTETNIYPPTLDLRGILRNLTSTSTYGQYARHLLAGELRPTAGKDNDNAHPPIHPVKAADKPELMARFRDFKYWLIYD
LVVRHFLATLSPPALIEEQRLTVDAGGVLFEASGLRIINDGYFTIYPFERPRANPLPLSALRIGMQVTVKDAKVVKRKTT
PPPYLSESELLRLMRKYGIGTDATMQDHIHTNVKRRYFKIIKGQCVPTPLGKALITSLSKYAPTLIDPNFRSRMESMLSL
IGSGKEMPDSVRRRLEEEAARVYTSMKPNSNQLGEELAKALRSMVNEKGA
>Mature_610_residues
MDKLIVAEKNSVAKAIAQYLAEGGYTLRRIGIVPVYFFKVNGEYWASMGLRGHILDFDFEHSYNNWNRVEPGKLLDLEPV
MVIRGWDRPYVTALVELSKQAREIILALDSDVEGEAIAYEVMLVTRLRKPTLRFRRALFSAVTRDDIRRAFSKLTTINVN
LARKVFTRMVIDLKYGATFTRLLTLSAKSSKAPLNRGEFLSYGPCQTPVLNLVVQRALERENFKPEVYYKIKLIIEANGE
LIELESIDKFKNLKEAQEALSRVKAGKAVVKNIEAKRVHVNPPKPLETVELERRASRFLNIRSKQTLDAAEELYRQGYIS
YPRTETNIYPPTLDLRGILRNLTSTSTYGQYARHLLAGELRPTAGKDNDNAHPPIHPVKAADKPELMARFRDFKYWLIYD
LVVRHFLATLSPPALIEEQRLTVDAGGVLFEASGLRIINDGYFTIYPFERPRANPLPLSALRIGMQVTVKDAKVVKRKTT
PPPYLSESELLRLMRKYGIGTDATMQDHIHTNVKRRYFKIIKGQCVPTPLGKALITSLSKYAPTLIDPNFRSRMESMLSL
IGSGKEMPDSVRRRLEEEAARVYTSMKPNSNQLGEELAKALRSMVNEKGA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=612, Percent_Identity=32.1895424836601, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI4507635, Length=580, Percent_Identity=32.2413793103448, Blast_Score=274, Evalue=2e-73,
Organism=Escherichia coli, GI1787529, Length=484, Percent_Identity=27.4793388429752, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788061, Length=617, Percent_Identity=25.4457050243112, Blast_Score=113, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=592, Percent_Identity=32.6013513513513, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI32563869, Length=600, Percent_Identity=32.8333333333333, Blast_Score=278, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6323263, Length=656, Percent_Identity=29.8780487804878, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24640096, Length=583, Percent_Identity=33.2761578044597, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24585251, Length=597, Percent_Identity=34.1708542713568, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 69375; Mature: 69375

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLIVAEKNSVAKAIAQYLAEGGYTLRRIGIVPVYFFKVNGEYWASMGLRGHILDFDFE
CCCEEEECCHHHHHHHHHHHHCCCCEEEEECEEEEEEEEECCHHHCCCCCCEEEEEEECC
HSYNNWNRVEPGKLLDLEPVMVIRGWDRPYVTALVELSKQAREIILALDSDVEGEAIAYE
CCCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHH
VMLVTRLRKPTLRFRRALFSAVTRDDIRRAFSKLTTINVNLARKVFTRMVIDLKYGATFT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCEE
RLLTLSAKSSKAPLNRGEFLSYGPCQTPVLNLVVQRALERENFKPEVYYKIKLIIEANGE
HHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
LIELESIDKFKNLKEAQEALSRVKAGKAVVKNIEAKRVHVNPPKPLETVELERRASRFLN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHC
IRSKQTLDAAEELYRQGYISYPRTETNIYPPTLDLRGILRNLTSTSTYGQYARHLLAGEL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
RPTAGKDNDNAHPPIHPVKAADKPELMARFRDFKYWLIYDLVVRHFLATLSPPALIEEQR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
LTVDAGGVLFEASGLRIINDGYFTIYPFERPRANPLPLSALRIGMQVTVKDAKVVKRKTT
EEEECCCEEEECCCCEEEECCEEEEEECCCCCCCCCCHHHHHCCEEEEECHHHHHHCCCC
PPPYLSESELLRLMRKYGIGTDATMQDHIHTNVKRRYFKIIKGQCVPTPLGKALITSLSK
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YAPTLIDPNFRSRMESMLSLIGSGKEMPDSVRRRLEEEAARVYTSMKPNSNQLGEELAKA
HCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LRSMVNEKGA
HHHHHHCCCC
>Mature Secondary Structure
MDKLIVAEKNSVAKAIAQYLAEGGYTLRRIGIVPVYFFKVNGEYWASMGLRGHILDFDFE
CCCEEEECCHHHHHHHHHHHHCCCCEEEEECEEEEEEEEECCHHHCCCCCCEEEEEEECC
HSYNNWNRVEPGKLLDLEPVMVIRGWDRPYVTALVELSKQAREIILALDSDVEGEAIAYE
CCCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHH
VMLVTRLRKPTLRFRRALFSAVTRDDIRRAFSKLTTINVNLARKVFTRMVIDLKYGATFT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCEE
RLLTLSAKSSKAPLNRGEFLSYGPCQTPVLNLVVQRALERENFKPEVYYKIKLIIEANGE
HHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
LIELESIDKFKNLKEAQEALSRVKAGKAVVKNIEAKRVHVNPPKPLETVELERRASRFLN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHC
IRSKQTLDAAEELYRQGYISYPRTETNIYPPTLDLRGILRNLTSTSTYGQYARHLLAGEL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
RPTAGKDNDNAHPPIHPVKAADKPELMARFRDFKYWLIYDLVVRHFLATLSPPALIEEQR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
LTVDAGGVLFEASGLRIINDGYFTIYPFERPRANPLPLSALRIGMQVTVKDAKVVKRKTT
EEEECCCEEEECCCCEEEECCEEEEEECCCCCCCCCCHHHHHCCEEEEECHHHHHHCCCC
PPPYLSESELLRLMRKYGIGTDATMQDHIHTNVKRRYFKIIKGQCVPTPLGKALITSLSK
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
YAPTLIDPNFRSRMESMLSLIGSGKEMPDSVRRRLEEEAARVYTSMKPNSNQLGEELAKA
HCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LRSMVNEKGA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]