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The map label for this gene is asnS [H]

Identifier: 159040656

GI number: 159040656

Start: 78338

End: 79384

Strand: Reverse

Name: asnS [H]

Synonym: Cmaq_0064

Alternate gene names: 159040656

Gene position: 79384-78338 (Counterclockwise)

Preceding gene: 159040657

Following gene: 159040655

Centisome position: 3.82

GC content: 45.65

Gene sequence:

>1047_bases
ATGAGCAAGGTTCATTCAGCTATACTCGAGTTTGAGAAGATGGTTAAGGATAAGGAATCGTACTTAAAGTTCATTGATGA
GTGGGTTAAGTACTCTTGGAAGTGGGCTAGGGAGAGTAAGTACGTTACAGTGTTTAAAATCCAAGCCTCCATACTTCACG
CTATTCGAGAATACTTAGATTCCCAGGGTTTCACAGAGGTTCTACCACCAATAATAGGGCCTGTCACCGACCCTGGAATA
AGGGGTGCTAAGCAAGCCACAGTGGACTTCTATGGTCATGAGTATAAGGTAATGTCCAGTGCAATACTGTATAAGCAGTA
TATGGCTGCAGTGCTTGGTAAAGTATACTTCGTGTCCCCAAACATTAGGTTTGAGCCGAATGATAGCGTATTCACTGGTA
GGCATCTTGTTGAATTCTTCCAAGTTGACTTAGAGATACTTAACGGTACACTTGATGATGCTATGAATGTTGCCGAGGGT
TTATTCATGTATGTTACTAGGTACATTAGGGATGTTCACGGCAAGGACCTTGAGAAAATGGGAAGGGAACTACCGCATTA
TAAGACCCCCTTCACCCGCTACACTCACGCGGAGGCTGTGGAGCTTGTTAATAAGCTTGGTTGCAGTAACCCAAGGAATA
CTGAGATACTTTGGGAATGCGAAAAAGTGCTTTCAGCTCACCACGATAGTCCACTCTTCATCTACGAGTACCCTAAGGGA
TCCAGGGGATTCTATAATAGGGAGGATCCAGGTAAGCCAGGTTACTTAAGGGACTTCGACATGCTTTACCCAGAGGGCTT
TGGTGAGGCTATTTCAGGCGGTGAACGTGAGTATGAGCCTGTTAAGGTAATTAGTAGGATAAGGGAGGCTGGGGAGGATC
CAAATAAGTACAGGTGGTTCCTTGAAATGCTTAAGGACTTCTACCCATTAAAAACCGCGGGCTTCGGCATAGGTGTTGAG
AGGTTCACTAGGTATATTTGCGGCCTTAGGGCTGTTTGGGAGGCTAGACCATACCCTAAGGTAGCTGGTGTAGGACCGGC
GCCATAG

Upstream 100 bases:

>100_bases
TTACCTAAATTATTAGGCTGATTAACCGTTAATTCACGTTAATATATTGTTAAATTGATGATAAGGTGTATAAGTAGACT
ACGGCAATGGGTGTGAAAGT

Downstream 100 bases:

>100_bases
GTGGTTTAATGTTTGAGTGGTTTGCATTAAAGGTTAGTGAACTTGCCCTCAGGGTGAAGGCAAGCCTAATAATGCTTAGA
GGTATTAGCGACTTCATTAA

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSKVHSAILEFEKMVKDKESYLKFIDEWVKYSWKWARESKYVTVFKIQASILHAIREYLDSQGFTEVLPPIIGPVTDPGI
RGAKQATVDFYGHEYKVMSSAILYKQYMAAVLGKVYFVSPNIRFEPNDSVFTGRHLVEFFQVDLEILNGTLDDAMNVAEG
LFMYVTRYIRDVHGKDLEKMGRELPHYKTPFTRYTHAEAVELVNKLGCSNPRNTEILWECEKVLSAHHDSPLFIYEYPKG
SRGFYNREDPGKPGYLRDFDMLYPEGFGEAISGGEREYEPVKVISRIREAGEDPNKYRWFLEMLKDFYPLKTAGFGIGVE
RFTRYICGLRAVWEARPYPKVAGVGPAP

Sequences:

>Translated_348_residues
MSKVHSAILEFEKMVKDKESYLKFIDEWVKYSWKWARESKYVTVFKIQASILHAIREYLDSQGFTEVLPPIIGPVTDPGI
RGAKQATVDFYGHEYKVMSSAILYKQYMAAVLGKVYFVSPNIRFEPNDSVFTGRHLVEFFQVDLEILNGTLDDAMNVAEG
LFMYVTRYIRDVHGKDLEKMGRELPHYKTPFTRYTHAEAVELVNKLGCSNPRNTEILWECEKVLSAHHDSPLFIYEYPKG
SRGFYNREDPGKPGYLRDFDMLYPEGFGEAISGGEREYEPVKVISRIREAGEDPNKYRWFLEMLKDFYPLKTAGFGIGVE
RFTRYICGLRAVWEARPYPKVAGVGPAP
>Mature_347_residues
SKVHSAILEFEKMVKDKESYLKFIDEWVKYSWKWARESKYVTVFKIQASILHAIREYLDSQGFTEVLPPIIGPVTDPGIR
GAKQATVDFYGHEYKVMSSAILYKQYMAAVLGKVYFVSPNIRFEPNDSVFTGRHLVEFFQVDLEILNGTLDDAMNVAEGL
FMYVTRYIRDVHGKDLEKMGRELPHYKTPFTRYTHAEAVELVNKLGCSNPRNTEILWECEKVLSAHHDSPLFIYEYPKGS
RGFYNREDPGKPGYLRDFDMLYPEGFGEAISGGEREYEPVKVISRIREAGEDPNKYRWFLEMLKDFYPLKTAGFGIGVER
FTRYICGLRAVWEARPYPKVAGVGPAP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=315, Percent_Identity=26.984126984127, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4758762, Length=304, Percent_Identity=23.6842105263158, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226491502, Length=330, Percent_Identity=26.0606060606061, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1787161, Length=332, Percent_Identity=25.3012048192771, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71984122, Length=310, Percent_Identity=26.4516129032258, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556372, Length=321, Percent_Identity=28.0373831775701, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=310, Percent_Identity=25.4838709677419, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=308, Percent_Identity=25.3246753246753, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323011, Length=337, Percent_Identity=25.2225519287834, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319872, Length=353, Percent_Identity=22.0963172804533, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17136276, Length=313, Percent_Identity=28.4345047923323, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922416, Length=316, Percent_Identity=23.1012658227848, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921528, Length=304, Percent_Identity=23.0263157894737, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 40190; Mature: 40059

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVHSAILEFEKMVKDKESYLKFIDEWVKYSWKWARESKYVTVFKIQASILHAIREYLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHC
SQGFTEVLPPIIGPVTDPGIRGAKQATVDFYGHEYKVMSSAILYKQYMAAVLGKVYFVSP
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECC
NIRFEPNDSVFTGRHLVEFFQVDLEILNGTLDDAMNVAEGLFMYVTRYIRDVHGKDLEKM
CCEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GRELPHYKTPFTRYTHAEAVELVNKLGCSNPRNTEILWECEKVLSAHHDSPLFIYEYPKG
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
SRGFYNREDPGKPGYLRDFDMLYPEGFGEAISGGEREYEPVKVISRIREAGEDPNKYRWF
CCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
LEMLKDFYPLKTAGFGIGVERFTRYICGLRAVWEARPYPKVAGVGPAP
HHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKVHSAILEFEKMVKDKESYLKFIDEWVKYSWKWARESKYVTVFKIQASILHAIREYLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHC
SQGFTEVLPPIIGPVTDPGIRGAKQATVDFYGHEYKVMSSAILYKQYMAAVLGKVYFVSP
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECC
NIRFEPNDSVFTGRHLVEFFQVDLEILNGTLDDAMNVAEGLFMYVTRYIRDVHGKDLEKM
CCEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GRELPHYKTPFTRYTHAEAVELVNKLGCSNPRNTEILWECEKVLSAHHDSPLFIYEYPKG
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
SRGFYNREDPGKPGYLRDFDMLYPEGFGEAISGGEREYEPVKVISRIREAGEDPNKYRWF
CCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
LEMLKDFYPLKTAGFGIGVERFTRYICGLRAVWEARPYPKVAGVGPAP
HHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]