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The map label for this gene is ilvE [H]

Identifier: 158425596

GI number: 158425596

Start: 4540726

End: 4541613

Strand: Reverse

Name: ilvE [H]

Synonym: AZC_3972

Alternate gene names: 158425596

Gene position: 4541613-4540726 (Counterclockwise)

Preceding gene: 158425601

Following gene: 158425589

Centisome position: 84.58

GC content: 63.85

Gene sequence:

>888_bases
ATGAGCGGAGAGCCTTTCGACAAACGTGACGGCTGGATCTGGATGGACGGCGCTCTGGTGCCGTGGCAGGACGCCAAGCT
GCACGTACTCTCCCATGGCCTTCACTATGCCAGCTGCGTGTTCGAAGGCGAGCGCGCCTATGGCGGCACGATCTTCAAGT
CCTCGGAGCATTCCGAGCGCCTGCGTCTCTCCGCCGAGATGCTGGACTTCACCATTCCCTATTCGATCGCCGAGATCGAT
GCGGCCAAGCGCCTGACGCTCGAGAAGAACAATCTCGTGGACGCCTATGTGCGTCCGGTCGCCTGGCGCGGCAGCGAGAT
GATGGGCGTCTCGGCCCAGCACAACAAGATCCACCTCGCCATCGGCGTGTGGGAATGGCCGAGCTATTTCGACCCCGAGC
AGCGTCAGAAGGGCCTGCGCCTCGACCTCGCGGAGTTCCGCCGCCCTGATCCGGCGACGATCCCCTGCAAGGCCAAGGCC
TCCGGTCTCTACATGATCTGCACCATCTCCAAGCATAAGGCCGAGCGTCGCGGCTATGCGGATGCGATGATGCTGGATTA
TCGCGGCCTCGTGGCGGAGTGCACCGGCGCCAACATCTTCTTCGTGAAGGACGGCGTCATCCACACGCCTAAGCCCGACT
GCTTCCTCGATGGCATCACCCGCCGCACGGTGATCGACCTTGCCAAGCGCCACGGCTACGAGGTGGTCGAGCGCCACATC
AAGCCTGAGGAACTGGCCGGCTTCTCCGAATGCTTCATCACCGGCACCGCAGCCGAGGTGACGCCGGTGGGCGAGATCGC
CGAGTGGAAGTTCACGCCGTCGGGCATCACCCATCAGCTGATGGATGCCTATACGGCCGAGACCCGCCCGAAGCAGGCCG
CCGCCTGA

Upstream 100 bases:

>100_bases
TTGAGTCCAGAAAATATCGACAACCTCGGCCCCTGCAAAGCGCGGGGGTGCGCAGGAGACAAGGGCCGCCCCGAAGACGA
GCCCAACAGGAGTGAAGATC

Downstream 100 bases:

>100_bases
TCGGGATCGCCTGATCCGCCGCCGAACGCGGCGCAGGACCAGAACCAGAGGGCCGCTCCGTGAACCGGAGCGGCCCTTTT
GCTTTGCGGTTGCGGAGCAG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSGEPFDKRDGWIWMDGALVPWQDAKLHVLSHGLHYASCVFEGERAYGGTIFKSSEHSERLRLSAEMLDFTIPYSIAEID
AAKRLTLEKNNLVDAYVRPVAWRGSEMMGVSAQHNKIHLAIGVWEWPSYFDPEQRQKGLRLDLAEFRRPDPATIPCKAKA
SGLYMICTISKHKAERRGYADAMMLDYRGLVAECTGANIFFVKDGVIHTPKPDCFLDGITRRTVIDLAKRHGYEVVERHI
KPEELAGFSECFITGTAAEVTPVGEIAEWKFTPSGITHQLMDAYTAETRPKQAAA

Sequences:

>Translated_295_residues
MSGEPFDKRDGWIWMDGALVPWQDAKLHVLSHGLHYASCVFEGERAYGGTIFKSSEHSERLRLSAEMLDFTIPYSIAEID
AAKRLTLEKNNLVDAYVRPVAWRGSEMMGVSAQHNKIHLAIGVWEWPSYFDPEQRQKGLRLDLAEFRRPDPATIPCKAKA
SGLYMICTISKHKAERRGYADAMMLDYRGLVAECTGANIFFVKDGVIHTPKPDCFLDGITRRTVIDLAKRHGYEVVERHI
KPEELAGFSECFITGTAAEVTPVGEIAEWKFTPSGITHQLMDAYTAETRPKQAAA
>Mature_294_residues
SGEPFDKRDGWIWMDGALVPWQDAKLHVLSHGLHYASCVFEGERAYGGTIFKSSEHSERLRLSAEMLDFTIPYSIAEIDA
AKRLTLEKNNLVDAYVRPVAWRGSEMMGVSAQHNKIHLAIGVWEWPSYFDPEQRQKGLRLDLAEFRRPDPATIPCKAKAS
GLYMICTISKHKAERRGYADAMMLDYRGLVAECTGANIFFVKDGVIHTPKPDCFLDGITRRTVIDLAKRHGYEVVERHIK
PEELAGFSECFITGTAAEVTPVGEIAEWKFTPSGITHQLMDAYTAETRPKQAAA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=297, Percent_Identity=38.047138047138, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787338, Length=256, Percent_Identity=25.390625, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33164; Mature: 33033

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEPFDKRDGWIWMDGALVPWQDAKLHVLSHGLHYASCVFEGERAYGGTIFKSSEHSER
CCCCCCCCCCCEEEECCEECCCCCCEEEEHHCCCEEEEEEEECCCCCCCEEEECCCCHHH
LRLSAEMLDFTIPYSIAEIDAAKRLTLEKNNLVDAYVRPVAWRGSEMMGVSAQHNKIHLA
HHHHHHHHEEECCCHHHHHCHHHEEEECCCCCHHHHHCCEEECCCCEEEEECCCCEEEEE
IGVWEWPSYFDPEQRQKGLRLDLAEFRRPDPATIPCKAKASGLYMICTISKHKAERRGYA
EEEECCCCCCCHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHCCCH
DAMMLDYRGLVAECTGANIFFVKDGVIHTPKPDCFLDGITRRTVIDLAKRHGYEVVERHI
HHEEEHHCCCEEEECCCEEEEEECCEEECCCCCHHHCCCHHHHHHHHHHHCCHHHHHHCC
KPEELAGFSECFITGTAAEVTPVGEIAEWKFTPSGITHQLMDAYTAETRPKQAAA
CHHHHCCCHHEEEECCCCCCCCCCCHHEEEECCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SGEPFDKRDGWIWMDGALVPWQDAKLHVLSHGLHYASCVFEGERAYGGTIFKSSEHSER
CCCCCCCCCCEEEECCEECCCCCCEEEEHHCCCEEEEEEEECCCCCCCEEEECCCCHHH
LRLSAEMLDFTIPYSIAEIDAAKRLTLEKNNLVDAYVRPVAWRGSEMMGVSAQHNKIHLA
HHHHHHHHEEECCCHHHHHCHHHEEEECCCCCHHHHHCCEEECCCCEEEEECCCCEEEEE
IGVWEWPSYFDPEQRQKGLRLDLAEFRRPDPATIPCKAKASGLYMICTISKHKAERRGYA
EEEECCCCCCCHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHCCCH
DAMMLDYRGLVAECTGANIFFVKDGVIHTPKPDCFLDGITRRTVIDLAKRHGYEVVERHI
HHEEEHHCCCEEEECCCEEEEEECCEEECCCCCHHHCCCHHHHHHHHHHHCCHHHHHHCC
KPEELAGFSECFITGTAAEVTPVGEIAEWKFTPSGITHQLMDAYTAETRPKQAAA
CHHHHCCCHHEEEECCCCCCCCCCCHHEEEECCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]