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The map label for this gene is 158425422

Identifier: 158425422

GI number: 158425422

Start: 4351880

End: 4352617

Strand: Reverse

Name: 158425422

Synonym: AZC_3798

Alternate gene names: NA

Gene position: 4352617-4351880 (Counterclockwise)

Preceding gene: 158425423

Following gene: 158425421

Centisome position: 81.06

GC content: 62.2

Gene sequence:

>738_bases
ATGAAACTCCAGCATTATCTTGGTGGTCTTGAAGGCCTCGGGCCTGTCAGTACCGAAACGCGCGTGTTCGTGGAGCCGTG
GGAAAAGCGCATCTTCGGTATCCACACCGCGATGATGGCGCTCTCCACCCAACTGTCGCTGCCGGCCACGCCCTCCTCCT
TCCGAACGGTTTGGACCTGGGCGGACCTTCGCAAGGGCGCAGAATCCCTCAATCCCCTCGATTACTTCCGCTTCCGCTAT
TATGAGAAGTGGATCGGCGGAATTTCCAGCTACTTCATTTCTCACGGCTATATCACCGAGGCTGAACTCGACGCGCTGAC
CAAGGAATACTACGCTGACTTCTCTAAGCCTCGGCCGTCGGCAGGGTGCGCGGTCGTCGACGCTCGCATCGTGCAGTATC
TGGTAGAGGGCGACAGCCCGAAACGCGGGGCGGCCGTGAATCACGCCTTTGCCGTGGGCGATGCGGTGGCGGTCTGCGAC
GTGCCCACCGTCGAGCACACCCGACTGCCGGGTTACCTGCGCAACCGCACCGGCACGGTGGAGACTGTCTACGAAGGAAC
CTACACCTATCTGTGCAACACCGGGCCGGACGGAATCGGCGCGGCCATGCCGGTCTATTGCGTCCGGTTCGACCCAGTGA
CGATCTGGCCCGGTAATGCCGAGCCGAACTTTGCCATCTATGCCGACCTCTACGCCCACTACATCGCCGCGCCTGCCGCC
GCGCAGCAGGCCGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCCCTTTTCGGCTTGGTCTCCGAGCGTGGGACCCAGATGCTGACGACCAAGACGCCGGCCAGTGCCTGATCACCTT
GCGTCAAAGGAAGCCTTCTA

Downstream 100 bases:

>100_bases
GTGGCGTGCACTCAACGGGGAGCCCCTTCCATGGCCGATCAGTTCAACTATCGCGAGGATCGCGAGGCCTACAGTGCCGC
CCGCGTACGCGCCCTTGAGG

Product: nitrile hydratase subunit beta

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKLQHYLGGLEGLGPVSTETRVFVEPWEKRIFGIHTAMMALSTQLSLPATPSSFRTVWTWADLRKGAESLNPLDYFRFRY
YEKWIGGISSYFISHGYITEAELDALTKEYYADFSKPRPSAGCAVVDARIVQYLVEGDSPKRGAAVNHAFAVGDAVAVCD
VPTVEHTRLPGYLRNRTGTVETVYEGTYTYLCNTGPDGIGAAMPVYCVRFDPVTIWPGNAEPNFAIYADLYAHYIAAPAA
AQQAA

Sequences:

>Translated_245_residues
MKLQHYLGGLEGLGPVSTETRVFVEPWEKRIFGIHTAMMALSTQLSLPATPSSFRTVWTWADLRKGAESLNPLDYFRFRY
YEKWIGGISSYFISHGYITEAELDALTKEYYADFSKPRPSAGCAVVDARIVQYLVEGDSPKRGAAVNHAFAVGDAVAVCD
VPTVEHTRLPGYLRNRTGTVETVYEGTYTYLCNTGPDGIGAAMPVYCVRFDPVTIWPGNAEPNFAIYADLYAHYIAAPAA
AQQAA
>Mature_245_residues
MKLQHYLGGLEGLGPVSTETRVFVEPWEKRIFGIHTAMMALSTQLSLPATPSSFRTVWTWADLRKGAESLNPLDYFRFRY
YEKWIGGISSYFISHGYITEAELDALTKEYYADFSKPRPSAGCAVVDARIVQYLVEGDSPKRGAAVNHAFAVGDAVAVCD
VPTVEHTRLPGYLRNRTGTVETVYEGTYTYLCNTGPDGIGAAMPVYCVRFDPVTIWPGNAEPNFAIYADLYAHYIAAPAA
AQQAA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 27026; Mature: 27026

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQHYLGGLEGLGPVSTETRVFVEPWEKRIFGIHTAMMALSTQLSLPATPSSFRTVWTW
CCCHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
ADLRKGAESLNPLDYFRFRYYEKWIGGISSYFISHGYITEAELDALTKEYYADFSKPRPS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
AGCAVVDARIVQYLVEGDSPKRGAAVNHAFAVGDAVAVCDVPTVEHTRLPGYLRNRTGTV
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCE
ETVYEGTYTYLCNTGPDGIGAAMPVYCVRFDPVTIWPGNAEPNFAIYADLYAHYIAAPAA
EEEECCEEEEEECCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEEEHHHHHHHHHCCCH
AQQAA
HHCCC
>Mature Secondary Structure
MKLQHYLGGLEGLGPVSTETRVFVEPWEKRIFGIHTAMMALSTQLSLPATPSSFRTVWTW
CCCHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
ADLRKGAESLNPLDYFRFRYYEKWIGGISSYFISHGYITEAELDALTKEYYADFSKPRPS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
AGCAVVDARIVQYLVEGDSPKRGAAVNHAFAVGDAVAVCDVPTVEHTRLPGYLRNRTGTV
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCCCE
ETVYEGTYTYLCNTGPDGIGAAMPVYCVRFDPVTIWPGNAEPNFAIYADLYAHYIAAPAA
EEEECCEEEEEECCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEEEHHHHHHHHHCCCH
AQQAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]