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The map label for this gene is 158425421

Identifier: 158425421

GI number: 158425421

Start: 4351232

End: 4351879

Strand: Reverse

Name: 158425421

Synonym: AZC_3797

Alternate gene names: NA

Gene position: 4351879-4351232 (Counterclockwise)

Preceding gene: 158425422

Following gene: 158425420

Centisome position: 81.04

GC content: 65.28

Gene sequence:

>648_bases
GTGGCGTGCACTCAACGGGGAGCCCCTTCCATGGCCGATCAGTTCAACTATCGCGAGGATCGCGAGGCCTACAGTGCCGC
CCGCGTACGCGCCCTTGAGGCTCTGCTCATCAAGAAGGGCATCATCACCGATCGATCGGTGGACACGGTGCTCGACTATT
TCGAAACGAAGATGGGGCCGTTCAACGGCGCCAAGATCGTAGCCCGCGCGTGGGTCGATTCCGCCTTCAAGGAACGTCTC
GTGGCCGACACGCCCAGCGCCATTGCTGAGTTGGCGCTGCCGGAGGGCATGGCGGGTGCCGAGGGCGAGCATATGCGGGC
GGTCGCCAATGCGCCGGGCGTCCACAACCTCATCATCTGTACCCTCTGCTCCTGCTATCCTTGGCCAGTGCTGGGTCTGC
CGCCCTACTGGTACAAAGACCCCACCTTCCGCAGCCGCGCGGCGCGCGAACCGAGGGCGGTTCTGAAAGAGTTCGGCCTC
GCCGTGCCCGAAAGCACGCGGGTGAAGGTGTGGGATTCCAGCGCCCAAATCCGCTGGTTCGTGGTGCCAGAGCGCCCTGC
CGGCACGGAGGGCTTGAGCGAGGCGGAGTTGGAAGTCCTCGTCACGCCAGAGGCGATGATCGGCGTTGCTGTCGCCCGGG
CGGCATAG

Upstream 100 bases:

>100_bases
GACGATCTGGCCCGGTAATGCCGAGCCGAACTTTGCCATCTATGCCGACCTCTACGCCCACTACATCGCCGCGCCTGCCG
CCGCGCAGCAGGCCGCGTGA

Downstream 100 bases:

>100_bases
GCGAGGGTCCGATGCTCACTAGCTTCGAGCAGTTTGCCGTTACCGAGATGATGGGGAAGACGGATACCCCGCCGCGCGCC
AATGGCACGCTGTGCTTCAG

Product: nitrile hydratase subunit alpha

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MACTQRGAPSMADQFNYREDREAYSAARVRALEALLIKKGIITDRSVDTVLDYFETKMGPFNGAKIVARAWVDSAFKERL
VADTPSAIAELALPEGMAGAEGEHMRAVANAPGVHNLIICTLCSCYPWPVLGLPPYWYKDPTFRSRAAREPRAVLKEFGL
AVPESTRVKVWDSSAQIRWFVVPERPAGTEGLSEAELEVLVTPEAMIGVAVARAA

Sequences:

>Translated_215_residues
MACTQRGAPSMADQFNYREDREAYSAARVRALEALLIKKGIITDRSVDTVLDYFETKMGPFNGAKIVARAWVDSAFKERL
VADTPSAIAELALPEGMAGAEGEHMRAVANAPGVHNLIICTLCSCYPWPVLGLPPYWYKDPTFRSRAAREPRAVLKEFGL
AVPESTRVKVWDSSAQIRWFVVPERPAGTEGLSEAELEVLVTPEAMIGVAVARAA
>Mature_214_residues
ACTQRGAPSMADQFNYREDREAYSAARVRALEALLIKKGIITDRSVDTVLDYFETKMGPFNGAKIVARAWVDSAFKERLV
ADTPSAIAELALPEGMAGAEGEHMRAVANAPGVHNLIICTLCSCYPWPVLGLPPYWYKDPTFRSRAAREPRAVLKEFGLA
VPESTRVKVWDSSAQIRWFVVPERPAGTEGLSEAELEVLVTPEAMIGVAVARAA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23539; Mature: 23408

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACTQRGAPSMADQFNYREDREAYSAARVRALEALLIKKGIITDRSVDTVLDYFETKMGP
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
FNGAKIVARAWVDSAFKERLVADTPSAIAELALPEGMAGAEGEHMRAVANAPGVHNLIIC
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHH
TLCSCYPWPVLGLPPYWYKDPTFRSRAAREPRAVLKEFGLAVPESTRVKVWDSSAQIRWF
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
VVPERPAGTEGLSEAELEVLVTPEAMIGVAVARAA
EEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
ACTQRGAPSMADQFNYREDREAYSAARVRALEALLIKKGIITDRSVDTVLDYFETKMGP
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
FNGAKIVARAWVDSAFKERLVADTPSAIAELALPEGMAGAEGEHMRAVANAPGVHNLIIC
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHH
TLCSCYPWPVLGLPPYWYKDPTFRSRAAREPRAVLKEFGLAVPESTRVKVWDSSAQIRWF
HHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEE
VVPERPAGTEGLSEAELEVLVTPEAMIGVAVARAA
EEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11700034 [H]