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The map label for this gene is dppA [H]

Identifier: 158425403

GI number: 158425403

Start: 4333538

End: 4335037

Strand: Direct

Name: dppA [H]

Synonym: AZC_3779

Alternate gene names: 158425403

Gene position: 4333538-4335037 (Clockwise)

Preceding gene: 158425402

Following gene: 158425404

Centisome position: 80.7

GC content: 65.8

Gene sequence:

>1500_bases
ATGAAGCGCTTTACGCCCGCCGCCTTCGCCGCTGCCCTTCTCGCCACCGTGACCCTGCCGGGGCTCGCAAAGGCCGAGAC
CGCGACCATCGCGCTCGCGGTGAACATCAACACGCTGGACCCGCACATGTCGGCGTCCTTCGGCTCCGACCTCAGCCTTC
TGAGCCACGTTTATCCGGCGCTGATCCTGCGCGGGCCGGACCTCAAGCTCCAGCCGGCGCTGGCCAAGAGCTGGACACCG
GTGAACGACACCACCTGGCGCTTCGAGCTGGTGCCGGGCGCTAAATTCGCCAATGGCGAAGCGCTGGACGCCAAGGCGGT
GCGGTGGAACCTCGACCGCGTGCGCGACCCCAAGGTGAATGCCCGCATCAAGTCGTGGTTCGATCTCGTGAAAGACGTGC
GGGTGATCGACCCGACCACGCTGGAAGTGGAGACGAGCGCGCCCTTCCCCGCCTTCGCCGACCAGCTGTCCATGTTCTTC
CTGCTGCCGCCGGAATGGGCCGCGACCCATAATCCGGCCACCGAGACCATGTCCGGCGGGCCCTACCAGATCACGGAGAA
CGTGCCCGGCGATCACATAACGCTGAAAGCCAACCCCACCTATTTCGGCGAGAAGCCGGCCTTCGACACCGCCATCTTCC
GCACCATTCCGGAGCCCTCGAGCCGCATCGCCGCGCTGCTGGCCGGCGAGGTGGACCTCATCACCGGCGTGCCGCCCTCC
GAGCTGAAGCGCATCAAGGACAGCGGCAAGGCCAATTCCGGCGCCACGCCCAGCACCCGCAGCGTCTTCATCAAGTTCAA
CACCCAGAAGGCGCCGCTCGACAACAAGCTGGTGCGCCAGGCGATGAACTATGCGGTCGACAAGACCGCCATCGGCGATT
CCATCTTCGAGGGCCTTGCCTACATCTCGCCCTGCGAGATCCTGACGCCCGCCTATTTCGGCTTCAATCCGGACCTGAAG
CCCTATGCCTATGACCCCGCCAAGGCCAAGGCGCTGCTGAAGCAGTCCGGCGCAAACCTCTCCCAGCCCATCGAGATGGA
CGTGCCGGTGGCGACCTATCTGCAGGGGCAGGAAGTGGCGCAAGTGGTGGCGGCGCAGCTGGGCGAAGTGGGCCTCAACG
TGAAAATCACGGAGATGGACTTCGGCGCCTACATGAACAAGTACCTGAAGTCCCGCACGCTGGCGCAGACCTCGCTGCTG
ACCCACGGCTGGCCCACCATCGACGCCGATGGCCAGCTCACCCTGCTCGCCCCCGGCAACCAGTATGCCTATTGGGACAA
TGCCGCCTTCGGCAAGGCGGTGGAGGCCGGGCGCTCCACCACCGACAAGGCCGCGCGCCTTGCGGCGTACAAGACCGCCA
CCTCCATCATGTGCGAGGAGGCGCCGGTGCTGTTCCTTTATGCCCAGCCCAACACCTATGGCACCTCCAAGCGCGTGGTG
TGGCAGCCCCGTGCCGACGACTGGGTGCGCGCCTACGACCTGAAGCCGGCGGCCAAGTGA

Upstream 100 bases:

>100_bases
ATTACGAGCGCGAGAAGCCCGCTCCCGACGCCACCTTGCAGGAGGTTGCGCCGGACCATTTCGTCCTCATCTCTCCCTGA
CCGCCCGACGGAGACGCCAG

Downstream 100 bases:

>100_bases
GCCGTCGTGACCGCTGACGAGCGGCTGCACCTCTTCGGCAGCCACTTCGGCACCTATGAGGTGGAGGCGGGCCCCGACGG
CCGCCCCCGCCTTCTGCCCT

Product: extracellular substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKRFTPAAFAAALLATVTLPGLAKAETATIALAVNINTLDPHMSASFGSDLSLLSHVYPALILRGPDLKLQPALAKSWTP
VNDTTWRFELVPGAKFANGEALDAKAVRWNLDRVRDPKVNARIKSWFDLVKDVRVIDPTTLEVETSAPFPAFADQLSMFF
LLPPEWAATHNPATETMSGGPYQITENVPGDHITLKANPTYFGEKPAFDTAIFRTIPEPSSRIAALLAGEVDLITGVPPS
ELKRIKDSGKANSGATPSTRSVFIKFNTQKAPLDNKLVRQAMNYAVDKTAIGDSIFEGLAYISPCEILTPAYFGFNPDLK
PYAYDPAKAKALLKQSGANLSQPIEMDVPVATYLQGQEVAQVVAAQLGEVGLNVKITEMDFGAYMNKYLKSRTLAQTSLL
THGWPTIDADGQLTLLAPGNQYAYWDNAAFGKAVEAGRSTTDKAARLAAYKTATSIMCEEAPVLFLYAQPNTYGTSKRVV
WQPRADDWVRAYDLKPAAK

Sequences:

>Translated_499_residues
MKRFTPAAFAAALLATVTLPGLAKAETATIALAVNINTLDPHMSASFGSDLSLLSHVYPALILRGPDLKLQPALAKSWTP
VNDTTWRFELVPGAKFANGEALDAKAVRWNLDRVRDPKVNARIKSWFDLVKDVRVIDPTTLEVETSAPFPAFADQLSMFF
LLPPEWAATHNPATETMSGGPYQITENVPGDHITLKANPTYFGEKPAFDTAIFRTIPEPSSRIAALLAGEVDLITGVPPS
ELKRIKDSGKANSGATPSTRSVFIKFNTQKAPLDNKLVRQAMNYAVDKTAIGDSIFEGLAYISPCEILTPAYFGFNPDLK
PYAYDPAKAKALLKQSGANLSQPIEMDVPVATYLQGQEVAQVVAAQLGEVGLNVKITEMDFGAYMNKYLKSRTLAQTSLL
THGWPTIDADGQLTLLAPGNQYAYWDNAAFGKAVEAGRSTTDKAARLAAYKTATSIMCEEAPVLFLYAQPNTYGTSKRVV
WQPRADDWVRAYDLKPAAK
>Mature_499_residues
MKRFTPAAFAAALLATVTLPGLAKAETATIALAVNINTLDPHMSASFGSDLSLLSHVYPALILRGPDLKLQPALAKSWTP
VNDTTWRFELVPGAKFANGEALDAKAVRWNLDRVRDPKVNARIKSWFDLVKDVRVIDPTTLEVETSAPFPAFADQLSMFF
LLPPEWAATHNPATETMSGGPYQITENVPGDHITLKANPTYFGEKPAFDTAIFRTIPEPSSRIAALLAGEVDLITGVPPS
ELKRIKDSGKANSGATPSTRSVFIKFNTQKAPLDNKLVRQAMNYAVDKTAIGDSIFEGLAYISPCEILTPAYFGFNPDLK
PYAYDPAKAKALLKQSGANLSQPIEMDVPVATYLQGQEVAQVVAAQLGEVGLNVKITEMDFGAYMNKYLKSRTLAQTSLL
THGWPTIDADGQLTLLAPGNQYAYWDNAAFGKAVEAGRSTTDKAARLAAYKTATSIMCEEAPVLFLYAQPNTYGTSKRVV
WQPRADDWVRAYDLKPAAK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=433, Percent_Identity=28.4064665127021, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789887, Length=395, Percent_Identity=29.873417721519, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787052, Length=492, Percent_Identity=27.0325203252033, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1787551, Length=467, Percent_Identity=25.4817987152034, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1787762, Length=522, Percent_Identity=24.1379310344828, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789397, Length=469, Percent_Identity=24.3070362473348, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081878, Length=516, Percent_Identity=22.093023255814, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787495, Length=506, Percent_Identity=21.9367588932806, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54262; Mature: 54262

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFTPAAFAAALLATVTLPGLAKAETATIALAVNINTLDPHMSASFGSDLSLLSHVYPA
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LILRGPDLKLQPALAKSWTPVNDTTWRFELVPGAKFANGEALDAKAVRWNLDRVRDPKVN
HEECCCCCEECCHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHEEEEHHHCCCCCCC
ARIKSWFDLVKDVRVIDPTTLEVETSAPFPAFADQLSMFFLLPPEWAATHNPATETMSGG
HHHHHHHHHHHCCEEECCCEEEEECCCCCHHHHHCCEEEEEECCCCCCCCCCCHHCCCCC
PYQITENVPGDHITLKANPTYFGEKPAFDTAIFRTIPEPSSRIAALLAGEVDLITGVPPS
CEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEECCCCHH
ELKRIKDSGKANSGATPSTRSVFIKFNTQKAPLDNKLVRQAMNYAVDKTAIGDSIFEGLA
HHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YISPCEILTPAYFGFNPDLKPYAYDPAKAKALLKQSGANLSQPIEMDVPVATYLQGQEVA
HCCCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHH
QVVAAQLGEVGLNVKITEMDFGAYMNKYLKSRTLAQTSLLTHGWPTIDADGQLTLLAPGN
HHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
QYAYWDNAAFGKAVEAGRSTTDKAARLAAYKTATSIMCEEAPVLFLYAQPNTYGTSKRVV
CEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCEEE
WQPRADDWVRAYDLKPAAK
ECCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKRFTPAAFAAALLATVTLPGLAKAETATIALAVNINTLDPHMSASFGSDLSLLSHVYPA
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
LILRGPDLKLQPALAKSWTPVNDTTWRFELVPGAKFANGEALDAKAVRWNLDRVRDPKVN
HEECCCCCEECCHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHEEEEHHHCCCCCCC
ARIKSWFDLVKDVRVIDPTTLEVETSAPFPAFADQLSMFFLLPPEWAATHNPATETMSGG
HHHHHHHHHHHCCEEECCCEEEEECCCCCHHHHHCCEEEEEECCCCCCCCCCCHHCCCCC
PYQITENVPGDHITLKANPTYFGEKPAFDTAIFRTIPEPSSRIAALLAGEVDLITGVPPS
CEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCEEEECCCCHH
ELKRIKDSGKANSGATPSTRSVFIKFNTQKAPLDNKLVRQAMNYAVDKTAIGDSIFEGLA
HHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YISPCEILTPAYFGFNPDLKPYAYDPAKAKALLKQSGANLSQPIEMDVPVATYLQGQEVA
HCCCHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHH
QVVAAQLGEVGLNVKITEMDFGAYMNKYLKSRTLAQTSLLTHGWPTIDADGQLTLLAPGN
HHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
QYAYWDNAAFGKAVEAGRSTTDKAARLAAYKTATSIMCEEAPVLFLYAQPNTYGTSKRVV
CEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCEEE
WQPRADDWVRAYDLKPAAK
ECCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]