Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 158425248

GI number: 158425248

Start: 4155859

End: 4156989

Strand: Reverse

Name: prfB [H]

Synonym: AZC_3624

Alternate gene names: 158425248

Gene position: 4156989-4155859 (Counterclockwise)

Preceding gene: 158425249

Following gene: 158425245

Centisome position: 77.41

GC content: 66.49

Gene sequence:

>1131_bases
ATGCGTGCGGAGCTCGCCGCGAAAGTCGACGAGATCAATGCTTCCGTTGATCTGCTGCGTCAGCATCTCAACGTGGACCG
CTCGCGCCGGCGCCTCGCGGAGCTCAACGCCATCGCCGAGGACCCCACCCTGTGGGACGATCCGGAGCGGGCGCAGAAGA
TCATGCAGGAGCGCACCTCCCTGGAGGATGCGCTGAACGCCATCGACCGCATCACCCGCGATCTCACCGACAATATCGAG
CTGATCGAGCTCGGTGAGGCGGAAGGCGACGAAAGCGTGATCACGGAAGCCGCCGGGGCGCTCGATGCCCTCAAGGCGGA
ACTGGCCCGCCGCGAGCTCGACGCCCTGCTCTCGGGCGAAGCTGATTCCAACGACACCTATCTGGAAGTCCATGCGGGTG
CCGGCGGTACCGACAGCCAGGACTGGGCCGAGATGCTGCTGCGCATGTACCGGCGCTGGTGTGAGCGGCACGGCTTCAAG
GTGGAACTGGTGGACGAGTCCGCCGGCGAACAGGCGGGCCTCAAGTCCGCGACCATCATCGTGAAAGGCCACAACGCCTA
TGGCTGGCTGAAGACCGAGGCCGGCGTGCACCGTCTGGTGCGCATCTCTCCCTTCGATTCCAATGCCCGCCGCCAGACCT
CCTTTGCCTCCATCGACGTCTATCCGGTCATCGACGACCGCATCGTGGTGGAGATCAATGAGAGTGACGTGCGCGTGGAC
ACCATGCGCTCCGGCGGCGCCGGCGGCCAGCACGTGAACAAGACCGAATCGGCGGTTCGACTGACCCACATTCCCACTGG
CATCGCCGTGGTCTCGCAGGGCGACCGGTCCCAGCACAAGAACCGCGCCACCGCGTGGAACATGCTGCGCGCCAAGCTGT
TCGAGATGGAGCTGAAGAAGCGCGAGGAGGCCGCCGCCGCCGAGCAGGCCGCGCGCACCGACATCGGCTGGGGCCACCAG
ATCCGCTCCTACGTGCTCCAGCCCTATCAGCTGGTGAAGGACCTGCGCACCGGTGTCACCTCGGGCACCCCGCAGGAGGT
GCTCGACGGCGATCTCGATCCCTTCATGGAAGCGGCCCTCGCCCAGCGCGCCTATGGCGGCGGGCCGGGCCAGGTCGAGG
ACGTGGAGTAA

Upstream 100 bases:

>100_bases
GGCCTCCTTGTGCTTTATCCGGCGCGCCACGGTGCCGGGCGTTTACACAGCGCCCCGTCGCCGCTACGTTCCGCCGCCCA
AATCCGGAGTTAGGTAAGCC

Downstream 100 bases:

>100_bases
GTCGTCCCGAGCGGACGGCGCCCCATCGAGAGCGCCGTCCGGTGAGGGGCGCGCAGTGCCTGAGCCCTTACCGCTTGCCG
GTCACGCCGGCAGGGGCGCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRAELAAKVDEINASVDLLRQHLNVDRSRRRLAELNAIAEDPTLWDDPERAQKIMQERTSLEDALNAIDRITRDLTDNIE
LIELGEAEGDESVITEAAGALDALKAELARRELDALLSGEADSNDTYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFK
VELVDESAGEQAGLKSATIIVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASIDVYPVIDDRIVVEINESDVRVD
TMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSQGDRSQHKNRATAWNMLRAKLFEMELKKREEAAAAEQAARTDIGWGHQ
IRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMEAALAQRAYGGGPGQVEDVE

Sequences:

>Translated_376_residues
MRAELAAKVDEINASVDLLRQHLNVDRSRRRLAELNAIAEDPTLWDDPERAQKIMQERTSLEDALNAIDRITRDLTDNIE
LIELGEAEGDESVITEAAGALDALKAELARRELDALLSGEADSNDTYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFK
VELVDESAGEQAGLKSATIIVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASIDVYPVIDDRIVVEINESDVRVD
TMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSQGDRSQHKNRATAWNMLRAKLFEMELKKREEAAAAEQAARTDIGWGHQ
IRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMEAALAQRAYGGGPGQVEDVE
>Mature_376_residues
MRAELAAKVDEINASVDLLRQHLNVDRSRRRLAELNAIAEDPTLWDDPERAQKIMQERTSLEDALNAIDRITRDLTDNIE
LIELGEAEGDESVITEAAGALDALKAELARRELDALLSGEADSNDTYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFK
VELVDESAGEQAGLKSATIIVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASIDVYPVIDDRIVVEINESDVRVD
TMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSQGDRSQHKNRATAWNMLRAKLFEMELKKREEAAAAEQAARTDIGWGHQ
IRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMEAALAQRAYGGGPGQVEDVE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=264, Percent_Identity=35.2272727272727, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI34577120, Length=263, Percent_Identity=35.361216730038, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI166795305, Length=176, Percent_Identity=35.7954545454545, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI2367172, Length=353, Percent_Identity=52.4079320113314, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=40.495867768595, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=207, Percent_Identity=33.8164251207729, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=241, Percent_Identity=36.9294605809129, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=40.8163265306122, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41592; Mature: 41592

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAELAAKVDEINASVDLLRQHLNVDRSRRRLAELNAIAEDPTLWDDPERAQKIMQERTS
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LEDALNAIDRITRDLTDNIELIELGEAEGDESVITEAAGALDALKAELARRELDALLSGE
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFKVELVDESAGEQAGLKSATII
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEE
VKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASIDVYPVIDDRIVVEINESDVRVD
EECCCCCEEEECHHHHEEEEEECCCCCCCHHCCCEEEEEEEEEECCEEEEEECCCCEEEE
TMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSQGDRSQHKNRATAWNMLRAKLFEMELKK
ECCCCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
REEAAAAEQAARTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMEAAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHH
AQRAYGGGPGQVEDVE
HHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRAELAAKVDEINASVDLLRQHLNVDRSRRRLAELNAIAEDPTLWDDPERAQKIMQERTS
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LEDALNAIDRITRDLTDNIELIELGEAEGDESVITEAAGALDALKAELARRELDALLSGE
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFKVELVDESAGEQAGLKSATII
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEE
VKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASIDVYPVIDDRIVVEINESDVRVD
EECCCCCEEEECHHHHEEEEEECCCCCCCHHCCCEEEEEEEEEECCEEEEEECCCCEEEE
TMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSQGDRSQHKNRATAWNMLRAKLFEMELKK
ECCCCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
REEAAAAEQAARTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMEAAL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHH
AQRAYGGGPGQVEDVE
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA