| Definition | Azorhizobium caulinodans ORS 571, complete genome. |
|---|---|
| Accession | NC_009937 |
| Length | 5,369,772 |
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The map label for this gene is mrcA [H]
Identifier: 158425249
GI number: 158425249
Start: 4157132
End: 4159582
Strand: Reverse
Name: mrcA [H]
Synonym: AZC_3625
Alternate gene names: 158425249
Gene position: 4159582-4157132 (Counterclockwise)
Preceding gene: 158425250
Following gene: 158425248
Centisome position: 77.46
GC content: 66.63
Gene sequence:
>2451_bases ATGCGGCTGGTGTTGAGGTTCTTCGGCTTCCTGTTTGCTCTCGGCACGCTGGCGCTCCTCGTCGGTGCGGCCGGTGCCAC CTATTTCGTGTGGAAATATTCCCAGGACCTGCCGGACTACACCCAGCTTCAGAATTACGAGCCCCCGGTGATGACCCGCG TCCATGCGGGCGACGGTTCGCTGGTGGCGGAATACGCCCGACAGAAGCGTCTCTACGTCCCCATCCAGTCCGTGCCCAAG CTCGTGGTCGAGGCCTTCCTCTCGGCCGAGGACAAGAATTTCTACGAGCACGGCGGCATCGACCCCACCGGCATCTTCCG CGCCGGCATGTTCTATCTGCAGAACCTCGGCACCAGCCGCCGTCCGCAGGGCGCCTCCACCATCACCCAGCAGGTGGCCA AGAACTTCCTCCTGACCAACGAGGTCTCCATCGACCGCAAGATCAAGGAAGCCCTGCTGGCGCTGCGCATCGAGCGCACC TATTCCAAGGACCGCATCCTCGAGCTGTACCTGAACGAGATCTATCTCGGCTCCGGCTCCTACGGCATCGCCGCCGCCGC GCTGACCTATTTCGACAAGTCGGTCGAGGATCTGAACATCCAGCAGGCGGCCTATCTCGCGGCCCTGCCCAAGGCGCCGT CCTCCTATAATCCCTATCGCAACACCGACCGTGCCATCGAACGCCGCAACTGGGTGATCGATCGCATGGCCGAGAACGGC TACATCACCGCCGCCCAGGCGGAGACGGCCAAGAAGAGCCCGCTCGAGGTCACCCCGCGCCCGACCGGCGCCCACATCTT CGCTGCCGAATATTATGCCGAGGAAGTGCGTCGCCAGATCTACGAGCGCTATGGCGAGACCAAGCTCTATGAGGGCGGCC TCTCGGTGCGGACCGCGCTCGACCCGCGCCTCCAGTCCATCGCCAAGAAAACGCTGATGGACGGCCTCATCCGCTTCGAC GAGCAGCGCGGCTGGCGCGGCCCGGTGACCAAGATCGAGTTCGCCGGCGACTGGGGTCCCAAGCTCGCCGAGGCCAAGAC CTTCTACGACATCCCCTGGAAGCTCGCGGTGGTGCTGGACGCCGGCAAGGACACCGCGCGCATCGGCCTCCAGCCGCCCA AGGACCGCTCCGGCGGCATCTCGAGCGCCCGCGACGTGGGCCTCATCAATCTGGACGGCGTGAAGTGGGCGCGCTGGGTG AAGGGACCCGATGCCGGCCGTGCCGTCTCGTCCGTCTCTCAGGTTCTGAAGACCGGCGACGTCATCTATGTGGAGGCCAT TCCCGGCAAGGACGGGCAGTATCGCCTGCACCAGATCCCGGAGATCGAGGGCGCCATCACGGTGATGGAGCCCCGCACCG GCCGCGTGCTCGCCATGGTGGGCGGCTTCTCCTATGATGAGAGCCAATTCAACCGCGCCACGCAGGCGCTGCGCCAGCCC GGCTCCTCCTTCAAGCCCATCGTCTATTCCGCCGCGCTGGACAATGGCTACACGCCCTCCACCGTGGTGCTGGACGCGCC CATCGAGATCGACCAGGGGCCCGGCCTCGGCGTGTGGGCGCCGGAGAATTACGCCAAGAAGTTCTACGGCCCGCAGACGC TGCGCTTCGGCCTCGAACAGTCGCGTAACGTGATGACCGTGCGCCTCGCGCAGGACATCGGCATGCCGCTGATCGCCGAT TACGCCAAGCGTTTCGGCGTCTATGACGACATGCTGCCGGTGCTCTCCATGTCGCTGGGCGCGGGCGAGACCACGCTTAT GCGCATGGTCGGCGCCTATGCGATCATCGCCAATGGCGGGCGCAAGGTGACGCCCTCGCTCATTGACCGCGTGCAGGACC GCTACGGCCACACCATCTTCCGGCATGACGAGCGCGAGTGCCGCGGCTGCGACGCCACCTCCTGGCAGAACCAGCCCGAG CCGACCCTGCTCGACCGCCGCCCCGTGGTGCTCGACGCCATGAGCACCTATCAGATCACCGCCATGATGGAGGGCGTGAT CCAGCGCGGCACCGGTACCGCGCTCAAGGATCTCGGCCGCCCCGTCGCCGGCAAGTCCGGCACGACCAATGACGAGAAGG ACGCCTGGTTCATCGCCTTCACCCCCGACATGGTGGTGGGCATGTATCTGGGCTACGACCGCCCCCGCGCCATGGGCAAG GGTTCGACCGGCGGCGTGCTCACCGCGCCCATCGTCCGCGACTTCATGAAGAGCGCGCTGGGCGACAAGCCGGCCGTTCC CTTCCGCGTGCCGCCGGGCATCAAGCTGGTGCGCATCAACCCGAAGACCGGCCTGCGCGCGGGTCCGGGTGAGGCGTCCA TCCTCGAAGGCTTCAAGCCCGGCACCGCCCCGCCCGACAGCTATTCCGTCATCGGCACCACGGACGCCTCCATCGGCGTC ACGCCGGAGGCGGATCGGGCGGTGCGCTCGGGCACGGGCGGCCTCTACTGA
Upstream 100 bases:
>100_bases CGGCGCCGCCTGGAACAGGCGTGCCCGTGGGTATCTTGTCGCCATTGGTCTGTTCGGCCGCCTCCCACAGGGGCCGGCTG TCGGGGAGGGAGCCGGCGAA
Downstream 100 bases:
>100_bases CCTGCCCCTTTGACGTTTCGACCGGGAGGTCCGCCGTGTGCGGGCCTCCTTGTGCTTTATCCGGCGCGCCACGGTGCCGG GCGTTTACACAGCGCCCCGT
Product: penicillin-binding protein 1A precursor
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 816; Mature: 816
Protein sequence:
>816_residues MRLVLRFFGFLFALGTLALLVGAAGATYFVWKYSQDLPDYTQLQNYEPPVMTRVHAGDGSLVAEYARQKRLYVPIQSVPK LVVEAFLSAEDKNFYEHGGIDPTGIFRAGMFYLQNLGTSRRPQGASTITQQVAKNFLLTNEVSIDRKIKEALLALRIERT YSKDRILELYLNEIYLGSGSYGIAAAALTYFDKSVEDLNIQQAAYLAALPKAPSSYNPYRNTDRAIERRNWVIDRMAENG YITAAQAETAKKSPLEVTPRPTGAHIFAAEYYAEEVRRQIYERYGETKLYEGGLSVRTALDPRLQSIAKKTLMDGLIRFD EQRGWRGPVTKIEFAGDWGPKLAEAKTFYDIPWKLAVVLDAGKDTARIGLQPPKDRSGGISSARDVGLINLDGVKWARWV KGPDAGRAVSSVSQVLKTGDVIYVEAIPGKDGQYRLHQIPEIEGAITVMEPRTGRVLAMVGGFSYDESQFNRATQALRQP GSSFKPIVYSAALDNGYTPSTVVLDAPIEIDQGPGLGVWAPENYAKKFYGPQTLRFGLEQSRNVMTVRLAQDIGMPLIAD YAKRFGVYDDMLPVLSMSLGAGETTLMRMVGAYAIIANGGRKVTPSLIDRVQDRYGHTIFRHDERECRGCDATSWQNQPE PTLLDRRPVVLDAMSTYQITAMMEGVIQRGTGTALKDLGRPVAGKSGTTNDEKDAWFIAFTPDMVVGMYLGYDRPRAMGK GSTGGVLTAPIVRDFMKSALGDKPAVPFRVPPGIKLVRINPKTGLRAGPGEASILEGFKPGTAPPDSYSVIGTTDASIGV TPEADRAVRSGTGGLY
Sequences:
>Translated_816_residues MRLVLRFFGFLFALGTLALLVGAAGATYFVWKYSQDLPDYTQLQNYEPPVMTRVHAGDGSLVAEYARQKRLYVPIQSVPK LVVEAFLSAEDKNFYEHGGIDPTGIFRAGMFYLQNLGTSRRPQGASTITQQVAKNFLLTNEVSIDRKIKEALLALRIERT YSKDRILELYLNEIYLGSGSYGIAAAALTYFDKSVEDLNIQQAAYLAALPKAPSSYNPYRNTDRAIERRNWVIDRMAENG YITAAQAETAKKSPLEVTPRPTGAHIFAAEYYAEEVRRQIYERYGETKLYEGGLSVRTALDPRLQSIAKKTLMDGLIRFD EQRGWRGPVTKIEFAGDWGPKLAEAKTFYDIPWKLAVVLDAGKDTARIGLQPPKDRSGGISSARDVGLINLDGVKWARWV KGPDAGRAVSSVSQVLKTGDVIYVEAIPGKDGQYRLHQIPEIEGAITVMEPRTGRVLAMVGGFSYDESQFNRATQALRQP GSSFKPIVYSAALDNGYTPSTVVLDAPIEIDQGPGLGVWAPENYAKKFYGPQTLRFGLEQSRNVMTVRLAQDIGMPLIAD YAKRFGVYDDMLPVLSMSLGAGETTLMRMVGAYAIIANGGRKVTPSLIDRVQDRYGHTIFRHDERECRGCDATSWQNQPE PTLLDRRPVVLDAMSTYQITAMMEGVIQRGTGTALKDLGRPVAGKSGTTNDEKDAWFIAFTPDMVVGMYLGYDRPRAMGK GSTGGVLTAPIVRDFMKSALGDKPAVPFRVPPGIKLVRINPKTGLRAGPGEASILEGFKPGTAPPDSYSVIGTTDASIGV TPEADRAVRSGTGGLY >Mature_816_residues MRLVLRFFGFLFALGTLALLVGAAGATYFVWKYSQDLPDYTQLQNYEPPVMTRVHAGDGSLVAEYARQKRLYVPIQSVPK LVVEAFLSAEDKNFYEHGGIDPTGIFRAGMFYLQNLGTSRRPQGASTITQQVAKNFLLTNEVSIDRKIKEALLALRIERT YSKDRILELYLNEIYLGSGSYGIAAAALTYFDKSVEDLNIQQAAYLAALPKAPSSYNPYRNTDRAIERRNWVIDRMAENG YITAAQAETAKKSPLEVTPRPTGAHIFAAEYYAEEVRRQIYERYGETKLYEGGLSVRTALDPRLQSIAKKTLMDGLIRFD EQRGWRGPVTKIEFAGDWGPKLAEAKTFYDIPWKLAVVLDAGKDTARIGLQPPKDRSGGISSARDVGLINLDGVKWARWV KGPDAGRAVSSVSQVLKTGDVIYVEAIPGKDGQYRLHQIPEIEGAITVMEPRTGRVLAMVGGFSYDESQFNRATQALRQP GSSFKPIVYSAALDNGYTPSTVVLDAPIEIDQGPGLGVWAPENYAKKFYGPQTLRFGLEQSRNVMTVRLAQDIGMPLIAD YAKRFGVYDDMLPVLSMSLGAGETTLMRMVGAYAIIANGGRKVTPSLIDRVQDRYGHTIFRHDERECRGCDATSWQNQPE PTLLDRRPVVLDAMSTYQITAMMEGVIQRGTGTALKDLGRPVAGKSGTTNDEKDAWFIAFTPDMVVGMYLGYDRPRAMGK GSTGGVLTAPIVRDFMKSALGDKPAVPFRVPPGIKLVRINPKTGLRAGPGEASILEGFKPGTAPPDSYSVIGTTDASIGV TPEADRAVRSGTGGLY
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=859, Percent_Identity=34.6915017462165, Blast_Score=445, Evalue=1e-126, Organism=Escherichia coli, GI1786343, Length=281, Percent_Identity=37.0106761565836, Blast_Score=162, Evalue=1e-40, Organism=Escherichia coli, GI1788867, Length=220, Percent_Identity=35.4545454545455, Blast_Score=117, Evalue=4e-27, Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=36.6863905325444, Blast_Score=95, Evalue=2e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 89642; Mature: 89642
Theoretical pI: Translated: 9.29; Mature: 9.29
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLVLRFFGFLFALGTLALLVGAAGATYFVWKYSQDLPDYTQLQNYEPPVMTRVHAGDGS CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCH LVAEYARQKRLYVPIQSVPKLVVEAFLSAEDKNFYEHGGIDPTGIFRAGMFYLQNLGTSR HHHHHHHCCEEEECHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCC RPQGASTITQQVAKNFLLTNEVSIDRKIKEALLALRIERTYSKDRILELYLNEIYLGSGS CCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHEEECCCC YGIAAAALTYFDKSVEDLNIQQAAYLAALPKAPSSYNPYRNTDRAIERRNWVIDRMAENG CHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC YITAAQAETAKKSPLEVTPRPTGAHIFAAEYYAEEVRRQIYERYGETKLYEGGLSVRTAL EEEEECCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECC DPRLQSIAKKTLMDGLIRFDEQRGWRGPVTKIEFAGDWGPKLAEAKTFYDIPWKLAVVLD CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHCCCEEECCCEEEEEEEE AGKDTARIGLQPPKDRSGGISSARDVGLINLDGVKWARWVKGPDAGRAVSSVSQVLKTGD CCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCC VIYVEAIPGKDGQYRLHQIPEIEGAITVMEPRTGRVLAMVGGFSYDESQFNRATQALRQP EEEEEEECCCCCCCHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCC GSSFKPIVYSAALDNGYTPSTVVLDAPIEIDQGPGLGVWAPENYAKKFYGPQTLRFGLEQ CCCCCCEEEEEECCCCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHC SRNVMTVRLAQDIGMPLIADYAKRFGVYDDMLPVLSMSLGAGETTLMRMVGAYAIIANGG CCCEEEEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCC RKVTPSLIDRVQDRYGHTIFRHDERECRGCDATSWQNQPEPTLLDRRPVVLDAMSTYQIT CEECHHHHHHHHHHHCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHH AMMEGVIQRGTGTALKDLGRPVAGKSGTTNDEKDAWFIAFTPDMVVGMYLGYDRPRAMGK HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCC GSTGGVLTAPIVRDFMKSALGDKPAVPFRVPPGIKLVRINPKTGLRAGPGEASILEGFKP CCCCCEEEHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHCCCCC GTAPPDSYSVIGTTDASIGVTPEADRAVRSGTGGLY CCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCC >Mature Secondary Structure MRLVLRFFGFLFALGTLALLVGAAGATYFVWKYSQDLPDYTQLQNYEPPVMTRVHAGDGS CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCH LVAEYARQKRLYVPIQSVPKLVVEAFLSAEDKNFYEHGGIDPTGIFRAGMFYLQNLGTSR HHHHHHHCCEEEECHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCC RPQGASTITQQVAKNFLLTNEVSIDRKIKEALLALRIERTYSKDRILELYLNEIYLGSGS CCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHEEECCCC YGIAAAALTYFDKSVEDLNIQQAAYLAALPKAPSSYNPYRNTDRAIERRNWVIDRMAENG CHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC YITAAQAETAKKSPLEVTPRPTGAHIFAAEYYAEEVRRQIYERYGETKLYEGGLSVRTAL EEEEECCCCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECC DPRLQSIAKKTLMDGLIRFDEQRGWRGPVTKIEFAGDWGPKLAEAKTFYDIPWKLAVVLD CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHCCCEEECCCEEEEEEEE AGKDTARIGLQPPKDRSGGISSARDVGLINLDGVKWARWVKGPDAGRAVSSVSQVLKTGD CCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCC VIYVEAIPGKDGQYRLHQIPEIEGAITVMEPRTGRVLAMVGGFSYDESQFNRATQALRQP EEEEEEECCCCCCCHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCC GSSFKPIVYSAALDNGYTPSTVVLDAPIEIDQGPGLGVWAPENYAKKFYGPQTLRFGLEQ CCCCCCEEEEEECCCCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHC SRNVMTVRLAQDIGMPLIADYAKRFGVYDDMLPVLSMSLGAGETTLMRMVGAYAIIANGG CCCEEEEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCC RKVTPSLIDRVQDRYGHTIFRHDERECRGCDATSWQNQPEPTLLDRRPVVLDAMSTYQIT CEECHHHHHHHHHHHCCEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHH AMMEGVIQRGTGTALKDLGRPVAGKSGTTNDEKDAWFIAFTPDMVVGMYLGYDRPRAMGK HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCC GSTGGVLTAPIVRDFMKSALGDKPAVPFRVPPGIKLVRINPKTGLRAGPGEASILEGFKP CCCCCEEEHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHCCCCC GTAPPDSYSVIGTTDASIGVTPEADRAVRSGTGGLY CCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11557893 [H]