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The map label for this gene is gcl [H]

Identifier: 158425112

GI number: 158425112

Start: 4004328

End: 4006103

Strand: Direct

Name: gcl [H]

Synonym: AZC_3488

Alternate gene names: 158425112

Gene position: 4004328-4006103 (Clockwise)

Preceding gene: 158425111

Following gene: 158425113

Centisome position: 74.57

GC content: 66.84

Gene sequence:

>1776_bases
ATGGCCCACATGCGTGCCGTCGATGCTGCCGTTCGCGTCCTGGAGAAGGAGGGCATCAGCTGCGCCTTCGGTGTTCCGGG
CGCCGCGATCAACCCGTTCTATTCCGCGCTGAAGGCGCGTGGTTCCATCCGCCACGTGCTCGCCCGGCACGTGGAAGGCG
CCTCCCACATGGCCGAGGGCTACACCCGGGCCGTCGCCGGCAACATCGGCGTGTGCATCGGCACCTCCGGTCCCGCCGGC
ACGGACATGATCACCGGCCTCTATTCGGCCTCGGCGGATTCCATCCCGATCCTGTGCATCACCGGCCAGGCCCCCCGCGC
CCGCCTCTATAAGGAAGACTTCCAGGCGGTCGATATCGAGAGCATCGCCAAGCCCGTCACCAAGTGGGCGGTCACGGTGC
GCGAGCCGGCGCTCGTTCCCATGGTGTTCCAGCAGGCGTTCCACGTCATGCGCTCGGGCCGCCCCGGCCCGGTGCTCATC
GACCTGCCGATCGACGTGCAGATGGCCGAGATCGAGTTCGACGAGGACACCTACGCCCCGCTCCCCGTTTACAAGCCCGC
CGCCACCCGCGCGCAGGCGGAAAAGGCCATCGCCATGCTGGCGAAGGCTGATCGCCCGCTCATCGTTTCCGGCGGCGGCA
TCATCAATGCGGATGCCTCCGACCTGCTGGTGGAGTTCGCGGAGCTCACCGGCGTGCCGGTCATCCCCACCCTCATGGGC
TGGGGCACCATTCCCGACGACCACCCGCTGATGGCCGGCATGTGCGGCCTCCAGACCTCGCACCGTTACGGCAATGCGAC
GATGCTGGCCTCCGATTTCGTGTTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCTCGGTGGAGGTCTACACCA
AGGGCCGCACCTTCATCCACGTGGACATCGAGCCGACCCAGATCGGCCGCGTCTTCGGCCCGGATTTCGGCATCGTCTCC
GATGCCGGGGCGGCGCTGAAGCTGTTCATCGAGGTGGCGAAGGAATGGAAGGCCGCCGGCAAGCTGCCGGACTGGTCCGC
CTGGGCCAAGGAATGCTTCGGCCGCAAGGAGACGATGCTGCGCAAGTCGCACTATGACAACGTGCCGCTGAAGCCGCAGC
GCGTCTATGAGGAGATGAACGAGGCGTTCGGCCGTGACGTGACCTATGTCACCACCATCGGCCTCTCGCAGATCGCCGGC
GCGCAGTTCCTGCATGTCCACCATCCGCGCCACTGGATCAACTGCGGGCAGGCCGGCCCGCTGGGCTGGACGCTGCCGGC
GGCTCTCGGCGTGCGCGCCGCGGACCCGGACCGGAAGATCGTGGCGCTCTCGGGCGACTATGACTTCCAGTTCCTCATCG
AGGAGCTGGCGGTGGGCGCCCAGCACAAGCTGCCCTACCTGCACGTGGTGGTGAACAACTCCTATCTCGGCCTCATCCGG
CAGGCCCAGCGCGGCTTCGAGATGGACTTCGAGGTGAGCCTCGCCTTCGAGAACATCAATTCGGAGGAAGCCGGCGCCTA
TGGCGTGGACCATGTGGCGGTGGCCGAGGGCCTCGGCTGCAAGGCCATCCGGGTGCGTACGCCGAATGAGTTCAAGGATG
CCTTCTCGCAGGCCGAGGCCCTGATGGAAGAGCATCAGGTGCCGGTGGTGATCGAGTTCATCCTGGAGCGGGTGACGAAC
ATCGCCATGGGCGTCGAGATCGACAAGGTCAACGAGTTCGAGGAGATCCTCGACCTGCCCCGCGCCACCGCGCGCGTGGT
GGAGCCGGCCGAGTAA

Upstream 100 bases:

>100_bases
GCATCAATGTGATGCCGTTTCTTGCCATAGCGAGGGCCAAGTTCGACCGATATACAATATACGAAAGTCGAACAAACCGG
TCCCAAGGGGAGGAAACGCC

Downstream 100 bases:

>100_bases
GCCCCGGTCTCCCATGTCTTACCTGCCGTGCCCTCCCGCGAGGCGCACGGCAGGCGTGCCGCGCGGGTGTCCGGTGCGGC
ATGTCCCGAGGCGCGGCGGC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAHMRAVDAAVRVLEKEGISCAFGVPGAAINPFYSALKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLI
DLPIDVQMAEIEFDEDTYAPLPVYKPAATRAQAEKAIAMLAKADRPLIVSGGGIINADASDLLVEFAELTGVPVIPTLMG
WGTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSVEVYTKGRTFIHVDIEPTQIGRVFGPDFGIVS
DAGAALKLFIEVAKEWKAAGKLPDWSAWAKECFGRKETMLRKSHYDNVPLKPQRVYEEMNEAFGRDVTYVTTIGLSQIAG
AQFLHVHHPRHWINCGQAGPLGWTLPAALGVRAADPDRKIVALSGDYDFQFLIEELAVGAQHKLPYLHVVVNNSYLGLIR
QAQRGFEMDFEVSLAFENINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFSQAEALMEEHQVPVVIEFILERVTN
IAMGVEIDKVNEFEEILDLPRATARVVEPAE

Sequences:

>Translated_591_residues
MAHMRAVDAAVRVLEKEGISCAFGVPGAAINPFYSALKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAG
TDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLI
DLPIDVQMAEIEFDEDTYAPLPVYKPAATRAQAEKAIAMLAKADRPLIVSGGGIINADASDLLVEFAELTGVPVIPTLMG
WGTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSVEVYTKGRTFIHVDIEPTQIGRVFGPDFGIVS
DAGAALKLFIEVAKEWKAAGKLPDWSAWAKECFGRKETMLRKSHYDNVPLKPQRVYEEMNEAFGRDVTYVTTIGLSQIAG
AQFLHVHHPRHWINCGQAGPLGWTLPAALGVRAADPDRKIVALSGDYDFQFLIEELAVGAQHKLPYLHVVVNNSYLGLIR
QAQRGFEMDFEVSLAFENINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFSQAEALMEEHQVPVVIEFILERVTN
IAMGVEIDKVNEFEEILDLPRATARVVEPAE
>Mature_590_residues
AHMRAVDAAVRVLEKEGISCAFGVPGAAINPFYSALKARGSIRHVLARHVEGASHMAEGYTRAVAGNIGVCIGTSGPAGT
DMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLID
LPIDVQMAEIEFDEDTYAPLPVYKPAATRAQAEKAIAMLAKADRPLIVSGGGIINADASDLLVEFAELTGVPVIPTLMGW
GTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSVEVYTKGRTFIHVDIEPTQIGRVFGPDFGIVSD
AGAALKLFIEVAKEWKAAGKLPDWSAWAKECFGRKETMLRKSHYDNVPLKPQRVYEEMNEAFGRDVTYVTTIGLSQIAGA
QFLHVHHPRHWINCGQAGPLGWTLPAALGVRAADPDRKIVALSGDYDFQFLIEELAVGAQHKLPYLHVVVNNSYLGLIRQ
AQRGFEMDFEVSLAFENINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFSQAEALMEEHQVPVVIEFILERVTNI
AMGVEIDKVNEFEEILDLPRATARVVEPAE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=24.1197183098592, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=24.496644295302, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI1786717, Length=578, Percent_Identity=76.4705882352941, Blast_Score=929, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=542, Percent_Identity=33.0258302583026, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=34.2436974789916, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=24.7755834829443, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=27.2916666666667, Blast_Score=139, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=24.4288224956063, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=24.4288224956063, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=31.1711711711712, Blast_Score=247, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6320816, Length=562, Percent_Identity=24.9110320284698, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922626, Length=517, Percent_Identity=27.0793036750484, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64337; Mature: 64206

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHMRAVDAAVRVLEKEGISCAFGVPGAAINPFYSALKARGSIRHVLARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
YTRAVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLIDLPIDVQMAEIEFDEDTYAP
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRAQAEKAIAMLAKADRPLIVSGGGIINADASDLLVEFAELTGVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSVEVYTKGRTFIH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDFGIVSDAGAALKLFIEVAKEWKAAGKLPDWSAWAKECFGRKETML
EECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
RKSHYDNVPLKPQRVYEEMNEAFGRDVTYVTTIGLSQIAGAQFLHVHHPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEEECCHHHHCCCCCCC
LGWTLPAALGVRAADPDRKIVALSGDYDFQFLIEELAVGAQHKLPYLHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
QAQRGFEMDFEVSLAFENINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFSQAEA
HHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LMEEHQVPVVIEFILERVTNIAMGVEIDKVNEFEEILDLPRATARVVEPAE
HHHHCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
AHMRAVDAAVRVLEKEGISCAFGVPGAAINPFYSALKARGSIRHVLARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHH
YTRAVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLYKEDFQAVDIE
HHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPMVFQQAFHVMRSGRPGPVLIDLPIDVQMAEIEFDEDTYAP
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAATRAQAEKAIAMLAKADRPLIVSGGGIINADASDLLVEFAELTGVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHCCCCCHHHHHC
WGTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSVEVYTKGRTFIH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDFGIVSDAGAALKLFIEVAKEWKAAGKLPDWSAWAKECFGRKETML
EECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHH
RKSHYDNVPLKPQRVYEEMNEAFGRDVTYVTTIGLSQIAGAQFLHVHHPRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEEECCHHHHCCCCCCC
LGWTLPAALGVRAADPDRKIVALSGDYDFQFLIEELAVGAQHKLPYLHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHH
QAQRGFEMDFEVSLAFENINSEEAGAYGVDHVAVAEGLGCKAIRVRTPNEFKDAFSQAEA
HHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LMEEHQVPVVIEFILERVTNIAMGVEIDKVNEFEEILDLPRATARVVEPAE
HHHHCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]