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The map label for this gene is ghrA [H]

Identifier: 158425089

GI number: 158425089

Start: 3980799

End: 3981740

Strand: Reverse

Name: ghrA [H]

Synonym: AZC_3465

Alternate gene names: 158425089

Gene position: 3981740-3980799 (Counterclockwise)

Preceding gene: 158425091

Following gene: 158425088

Centisome position: 74.15

GC content: 67.41

Gene sequence:

>942_bases
TTGCGTGAGTCTCTTGTTTTCTACAGTCCCGCCGACGTGGCCGCCGATTGGCTGGCGGCCATCGGCGAGCATCTCCCGGG
CCTCGACATGCACATCGCCACCCGCGAGGGCGCGCCGTGTCCGGCGGAGGATGTGCACTACGCGGTGGTGTGGAAGCCGC
CGCAGGACTTTTTCAAGCCCTTCACGAACCTGAAGCTGGTGGTGAACCTCGGGGCCGGCGTCGATGCACTCGTCTCGCGT
ACCGACCTGCCGGACGTGCCGATCACCCGCCTGTCCGACCCCGAAATGTCGAAGATGATGGCCGGCTTCGTGCTCATGTG
CGTGCTGCGCCATGCCCGTGACATCGTGGCCTTCGAGCGCATCCAGAAGGAAGGCCGCTGGCGCTACATCCATCCCACCA
CGCCGGACCGCATCCGCGTGGGTGTGATGGGCCTTGGCGAACTGGGTGCCACCGCCGCGCACGAAATCGCCCGTTTCGGC
TATCCGGTGAAGGGCTGGTCCCGCACGCCCAAGGCACTGCCCGGCATCGAATGCCACCACGGCCTCGAGGCTCTGCCGCA
GTTTCTCGCGCAGACCGATATCCTCGTGGTGATGCTGCCCCTCACTCCGGAGACCCGGCACATCGTCAATGCCGAGCGTC
TCGCGATGCTGCCGAAGGGGGCGAAATTCATCAATGTCGCCCGCGGCCCCATCGTGGACGAGCCGGCGCTGGTGGCGGCG
CTCCAGTCCGGCCATATCTCGGAAGCCTCCCTCGACGTGTTCGAGGAGGAGCCGCTGCCGGAGGCGAGCCCGCTCTGGGG
CATGGACAACGTGCTCATCACGCCGCACCTCGCCTCCATTGCCCTGCCGAGGTCGGCGGCGGCGCAGATCGCGGAGAACA
TCCGCCGCATCCGCGCGGGCCAGCCGGTGCTGAACCGCGTCGATCCCTCCCGCGGCTACTGA

Upstream 100 bases:

>100_bases
TGTGGGCGCCGAATCGTGGCGGCGCGGGGGGCGCTCGGCTATGAACGGACCATCACCGGCGCAGGAAGCGGCGGAAGAAC
GGATCAGAGGGTGAACGGCG

Downstream 100 bases:

>100_bases
CCGGCGCGGGGCGGCGACGGTCGCCCCGCTGACGCCGCCGTGATCGGAAAAAGCGTCTCAACCCCCTGACAAACGCAGCT
CGGTCTTCTTATATGAGCCT

Product: dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRESLVFYSPADVAADWLAAIGEHLPGLDMHIATREGAPCPAEDVHYAVVWKPPQDFFKPFTNLKLVVNLGAGVDALVSR
TDLPDVPITRLSDPEMSKMMAGFVLMCVLRHARDIVAFERIQKEGRWRYIHPTTPDRIRVGVMGLGELGATAAHEIARFG
YPVKGWSRTPKALPGIECHHGLEALPQFLAQTDILVVMLPLTPETRHIVNAERLAMLPKGAKFINVARGPIVDEPALVAA
LQSGHISEASLDVFEEEPLPEASPLWGMDNVLITPHLASIALPRSAAAQIAENIRRIRAGQPVLNRVDPSRGY

Sequences:

>Translated_313_residues
MRESLVFYSPADVAADWLAAIGEHLPGLDMHIATREGAPCPAEDVHYAVVWKPPQDFFKPFTNLKLVVNLGAGVDALVSR
TDLPDVPITRLSDPEMSKMMAGFVLMCVLRHARDIVAFERIQKEGRWRYIHPTTPDRIRVGVMGLGELGATAAHEIARFG
YPVKGWSRTPKALPGIECHHGLEALPQFLAQTDILVVMLPLTPETRHIVNAERLAMLPKGAKFINVARGPIVDEPALVAA
LQSGHISEASLDVFEEEPLPEASPLWGMDNVLITPHLASIALPRSAAAQIAENIRRIRAGQPVLNRVDPSRGY
>Mature_313_residues
MRESLVFYSPADVAADWLAAIGEHLPGLDMHIATREGAPCPAEDVHYAVVWKPPQDFFKPFTNLKLVVNLGAGVDALVSR
TDLPDVPITRLSDPEMSKMMAGFVLMCVLRHARDIVAFERIQKEGRWRYIHPTTPDRIRVGVMGLGELGATAAHEIARFG
YPVKGWSRTPKALPGIECHHGLEALPQFLAQTDILVVMLPLTPETRHIVNAERLAMLPKGAKFINVARGPIVDEPALVAA
LQSGHISEASLDVFEEEPLPEASPLWGMDNVLITPHLASIALPRSAAAQIAENIRRIRAGQPVLNRVDPSRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI6912396, Length=225, Percent_Identity=30.2222222222222, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI145580578, Length=274, Percent_Identity=27.007299270073, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4557499, Length=274, Percent_Identity=27.007299270073, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=27.4074074074074, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=27.4074074074074, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI145580575, Length=260, Percent_Identity=27.3076923076923, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87081824, Length=315, Percent_Identity=35.8730158730159, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87082289, Length=260, Percent_Identity=34.6153846153846, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789279, Length=144, Percent_Identity=31.9444444444444, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787645, Length=182, Percent_Identity=24.7252747252747, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=32.4, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=144, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324055, Length=251, Percent_Identity=29.4820717131474, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325144, Length=183, Percent_Identity=27.3224043715847, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322116, Length=151, Percent_Identity=26.4900662251656, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=151, Percent_Identity=26.4900662251656, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921140, Length=228, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28571528, Length=177, Percent_Identity=35.0282485875706, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574286, Length=172, Percent_Identity=33.1395348837209, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585516, Length=173, Percent_Identity=32.9479768786127, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=26.5625, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=26.5625, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=26.5625, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=26.5625, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=26.5625, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24646446, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646448, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646452, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646450, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472511, Length=279, Percent_Identity=25.4480286738351, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34317; Mature: 34317

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRESLVFYSPADVAADWLAAIGEHLPGLDMHIATREGAPCPAEDVHYAVVWKPPQDFFKP
CCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHH
FTNLKLVVNLGAGVDALVSRTDLPDVPITRLSDPEMSKMMAGFVLMCVLRHARDIVAFER
HCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQKEGRWRYIHPTTPDRIRVGVMGLGELGATAAHEIARFGYPVKGWSRTPKALPGIECHH
HHHCCCEEEECCCCCCCEEEEEEECCHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
GLEALPQFLAQTDILVVMLPLTPETRHIVNAERLAMLPKGAKFINVARGPIVDEPALVAA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHCHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LQSGHISEASLDVFEEEPLPEASPLWGMDNVLITPHLASIALPRSAAAQIAENIRRIRAG
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHCC
QPVLNRVDPSRGY
CHHHHHCCCCCCC
>Mature Secondary Structure
MRESLVFYSPADVAADWLAAIGEHLPGLDMHIATREGAPCPAEDVHYAVVWKPPQDFFKP
CCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHH
FTNLKLVVNLGAGVDALVSRTDLPDVPITRLSDPEMSKMMAGFVLMCVLRHARDIVAFER
HCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQKEGRWRYIHPTTPDRIRVGVMGLGELGATAAHEIARFGYPVKGWSRTPKALPGIECHH
HHHCCCEEEECCCCCCCEEEEEEECCHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
GLEALPQFLAQTDILVVMLPLTPETRHIVNAERLAMLPKGAKFINVARGPIVDEPALVAA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHCHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LQSGHISEASLDVFEEEPLPEASPLWGMDNVLITPHLASIALPRSAAAQIAENIRRIRAG
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHCC
QPVLNRVDPSRGY
CHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA