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The map label for this gene is fixB

Identifier: 158425072

GI number: 158425072

Start: 3963462

End: 3964571

Strand: Direct

Name: fixB

Synonym: AZC_3448

Alternate gene names: 158425072

Gene position: 3963462-3964571 (Clockwise)

Preceding gene: 158425071

Following gene: 158425073

Centisome position: 73.81

GC content: 66.4

Gene sequence:

>1110_bases
ATGAGCGAACCCACCAAAGCACCCGCCGCGTCCGCCGGAAGTCGCGCCGCGACGAAGAAGGAACTGCCCGAGCACTTCAA
GGCCTATAAGCACGTCTGGGTGTTCATCGAGCAGGAACGCGGACAGGTCCATCCGGTCTCGTGGGAGCTGATGGGCGCCG
GCCGCAAGCTCGCCGACAAGCTCAAGGTGGAGCTGGCGGCCATCGTGCTGGGGCCGGACGACGAGGCGACGCAGCACGCC
GCCGCCGAAGCCTTCAGCTATGGCGCCGACCTCACTTATGTGGTGGCCGATCCCCTGCTCACCGACTATCGCAACGAGGC
CTATACCAAGGCCCTCACCGATGTTGTGAACACTTACAAGCCAGAGATCCTGCTGCTGGGTGCGACCACGCTCGGCCGTG
ACCTTGCCGGCTCGGTCGCCACCACGCTGCTCACCGGCCTCACCGCTGACTGCACGGAGCTCGACGTGGATGCGGACGGC
TCGCTCGCCGCCACCCGCCCCACCTTCGGTGGCTCCCTGCTTTGCACCATCTACACGCTAAACTACCGGCCGCAGATGGC
GACGGTGCGTCCGCGCGTGATGCCCATGCCTGAACGCGAGGAAAAGCCCATCGGCCGCATCATCACCCACCCGCTGGGGA
TGGTGGAGGACGATATCGTCACCAAGATCCTGTCCTTCCTGCCGGATCGCGATTCCGCCAAGTCCAACCTCGCCTATGCG
GACGTGGTGGTGGCGGGCGGCCTCGGCCTCGGCTCGCCGGAGAATTTCCAGCTCGTGCGCCAGCTCGCCGGCGTGCTGGG
GGCGGAATATGGCTGCTCCCGCCCGCTGGTGCAGAAGGGCTGGGTCACGTCTGACCGGCAGATCGGCCAGACGGGCAAGA
CGATCCGGCCCAAGCTCTATATCGCCGCCGGCATTTCGGGCGCCATCCAGCACCGTGTGGGCGTCGAGGGCGCGGACATG
ATCGTCGCCATCAACACCGACAAGAACGCCCCCATCTTCGATTTCGCCCATGTGGGCATTGTCACCGATGCGATCCGGCT
GCTGCCGGCGCTGACCGAAGCCTTCCGCGCCCGCCTCTCCCCCCATTCGCGCGACCGGATCGCCAGCTGA

Upstream 100 bases:

>100_bases
GACGCCCTGATCGCGGAAATCTTCAAGCGCCAGCCCAAGCTCGAGGACGACCTCGCGGCCCTGGCGCGCGGCTACTGACG
GACAGACGAGGGAGCACGTC

Downstream 100 bases:

>100_bases
AGGAGCGTGCCATGATTGATGAGAAGTTCGACGCCATCGTCGTCGGCGCGGGCATGGCGGGCAACGCCGCCACGCTCACC
CTCGCCCGGCGCGGCCTGAA

Product: FixB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSEPTKAPAASAGSRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVELAAIVLGPDDEATQHA
AAEAFSYGADLTYVVADPLLTDYRNEAYTKALTDVVNTYKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADG
SLAATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPEREEKPIGRIITHPLGMVEDDIVTKILSFLPDRDSAKSNLAYA
DVVVAGGLGLGSPENFQLVRQLAGVLGAEYGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADM
IVAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFRARLSPHSRDRIAS

Sequences:

>Translated_369_residues
MSEPTKAPAASAGSRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVELAAIVLGPDDEATQHA
AAEAFSYGADLTYVVADPLLTDYRNEAYTKALTDVVNTYKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADG
SLAATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPEREEKPIGRIITHPLGMVEDDIVTKILSFLPDRDSAKSNLAYA
DVVVAGGLGLGSPENFQLVRQLAGVLGAEYGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADM
IVAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFRARLSPHSRDRIAS
>Mature_368_residues
SEPTKAPAASAGSRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVELAAIVLGPDDEATQHAA
AEAFSYGADLTYVVADPLLTDYRNEAYTKALTDVVNTYKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGS
LAATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPEREEKPIGRIITHPLGMVEDDIVTKILSFLPDRDSAKSNLAYAD
VVVAGGLGLGSPENFQLVRQLAGVLGAEYGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADMI
VAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFRARLSPHSRDRIAS

Specific function: May play a role in a redox process involved in nitrogen fixation

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family

Homologues:

Organism=Homo sapiens, GI4503607, Length=127, Percent_Identity=50.3937007874016, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI189181759, Length=127, Percent_Identity=50.3937007874016, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1787990, Length=328, Percent_Identity=33.2317073170732, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786226, Length=338, Percent_Identity=30.1775147928994, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI87082157, Length=260, Percent_Identity=28.8461538461538, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17506929, Length=331, Percent_Identity=32.3262839879154, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325261, Length=333, Percent_Identity=30.03003003003, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17136898, Length=325, Percent_Identity=32, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24652801, Length=325, Percent_Identity=32, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXB_AZOC5 (P26483)

Other databases:

- EMBL:   X55450
- EMBL:   AP009384
- PIR:   S14071
- RefSeq:   YP_001526364.1
- ProteinModelPortal:   P26483
- SMR:   P26483
- GeneID:   5690797
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3448
- HOGENOM:   HBG615986
- OMA:   EGVMKKE
- ProtClustDB:   CLSK864406
- BioCyc:   ACAU438753:AZC_3448-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF000089
- SMART:   SM00893

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha

EC number: NA

Molecular weight: Translated: 39704; Mature: 39573

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPTKAPAASAGSRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCHHHHHH
LKVELAAIVLGPDDEATQHAAAEAFSYGADLTYVVADPLLTDYRNEAYTKALTDVVNTYK
HHEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGSLAATRPTFGGSLLCTIYTL
CCEEEEECHHHCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEEE
NYRPQMATVRPRVMPMPEREEKPIGRIITHPLGMVEDDIVTKILSFLPDRDSAKSNLAYA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
DVVVAGGLGLGSPENFQLVRQLAGVLGAEYGCSRPLVQKGWVTSDRQIGQTGKTIRPKLY
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCEEE
IAAGISGAIQHRVGVEGADMIVAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFRARLS
EEECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
PHSRDRIAS
CCCCCCCCC
>Mature Secondary Structure 
SEPTKAPAASAGSRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCHHHHHH
LKVELAAIVLGPDDEATQHAAAEAFSYGADLTYVVADPLLTDYRNEAYTKALTDVVNTYK
HHEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGSLAATRPTFGGSLLCTIYTL
CCEEEEECHHHCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEEE
NYRPQMATVRPRVMPMPEREEKPIGRIITHPLGMVEDDIVTKILSFLPDRDSAKSNLAYA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
DVVVAGGLGLGSPENFQLVRQLAGVLGAEYGCSRPLVQKGWVTSDRQIGQTGKTIRPKLY
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCEEE
IAAGISGAIQHRVGVEGADMIVAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFRARLS
EEECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
PHSRDRIAS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088