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The map label for this gene is fixA

Identifier: 158425071

GI number: 158425071

Start: 3962594

End: 3963439

Strand: Direct

Name: fixA

Synonym: AZC_3447

Alternate gene names: 158425071

Gene position: 3962594-3963439 (Clockwise)

Preceding gene: 158425070

Following gene: 158425072

Centisome position: 73.79

GC content: 68.91

Gene sequence:

>846_bases
ATGCACATCGTCGTCTGCATCAAGCAGGTTCCTGACTCCGCGCAGATCCGCGTGCACCCCGTGACGAACACCATCATGCG
TCAGGGTGTGCCCACGATCATCAACCCTTACGACCTCTTCGCGCTGGAGGCCGCGCTCGCGCTGCGCGACCAGCATGGCG
GCGAGGTCACGGTCCTCACCATGGGCCCGCCCTCGGCCGAGGACAGCCTGCGCAAGGCACTCACCTTCGGCGCCGACCGG
GCCGTGCTGCTCACGGACCGCTTCTTCGCCGGCTCCGACACGCTCGCCACCACCTATGCGCTGGCCACCGCCGTGCGGAA
GATCGGCGAGACCTTCGGCGCGCCGGACATCGTGTTCACCGGCAAACAGACCATCGACGGTGACACGGCGCAGGTCGGCC
CCGGCATCGCCAAGCGGCTCGGCCTGCTCCAGCTCACCTATGTGGCGAAGATCGCCAATGTCGATCTTGCGGGCCGCAGC
ATCCAGGTGGAGCGGCGGTCCGAGGGCGGCGTGCAGGTCCTTCAGACCCGCCTGCCCTGCCTCATCACCATGCTGGAGGC
CACCAACGAGATCCGCCGCGGCGCCATGGCCGATGCGCTGCGCGCCGCCCGCGCCGAGGTCGTGATCTGGAGCGCCAAGG
ACGCCGGCGTCGAGGACATCTCCAAATGCGGCCTGCGCGGTTCGCCCACCATCGTGAAGCGCGTGTTTGCGCCCTCCGCC
CGCGCGGAGAAGGCGACGATGGTGGACTTCAGCGAAACCGCGCCGGCGGACGCCCTGATCGCGGAAATCTTCAAGCGCCA
GCCCAAGCTCGAGGACGACCTCGCGGCCCTGGCGCGCGGCTACTGA

Upstream 100 bases:

>100_bases
ACAGCCCTTTGATTTCATGCGCGTTTTCGGGCTGAAAGACAGTTGGTACGACACTTGCTCATTCCTCCCCAAGAGCCCAA
CCGTTCCGGGAGCGAACGCA

Downstream 100 bases:

>100_bases
CGGACAGACGAGGGAGCACGTCATGAGCGAACCCACCAAAGCACCCGCCGCGTCCGCCGGAAGTCGCGCCGCGACGAAGA
AGGAACTGCCCGAGCACTTC

Product: FixA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALALRDQHGGEVTVLTMGPPSAEDSLRKALTFGADR
AVLLTDRFFAGSDTLATTYALATAVRKIGETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKIANVDLAGRS
IQVERRSEGGVQVLQTRLPCLITMLEATNEIRRGAMADALRAARAEVVIWSAKDAGVEDISKCGLRGSPTIVKRVFAPSA
RAEKATMVDFSETAPADALIAEIFKRQPKLEDDLAALARGY

Sequences:

>Translated_281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALALRDQHGGEVTVLTMGPPSAEDSLRKALTFGADR
AVLLTDRFFAGSDTLATTYALATAVRKIGETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKIANVDLAGRS
IQVERRSEGGVQVLQTRLPCLITMLEATNEIRRGAMADALRAARAEVVIWSAKDAGVEDISKCGLRGSPTIVKRVFAPSA
RAEKATMVDFSETAPADALIAEIFKRQPKLEDDLAALARGY
>Mature_281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALALRDQHGGEVTVLTMGPPSAEDSLRKALTFGADR
AVLLTDRFFAGSDTLATTYALATAVRKIGETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKIANVDLAGRS
IQVERRSEGGVQVLQTRLPCLITMLEATNEIRRGAMADALRAARAEVVIWSAKDAGVEDISKCGLRGSPTIVKRVFAPSA
RAEKATMVDFSETAPADALIAEIFKRQPKLEDDLAALARGY

Specific function: May play a role in a redox process involved in nitrogen fixation

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family

Homologues:

Organism=Homo sapiens, GI4503609, Length=224, Percent_Identity=29.9107142857143, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI62420877, Length=211, Percent_Identity=29.8578199052133, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI87081682, Length=219, Percent_Identity=28.310502283105, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367123, Length=218, Percent_Identity=30.2752293577982, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25141345, Length=224, Percent_Identity=32.5892857142857, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321646, Length=187, Percent_Identity=31.0160427807487, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651147, Length=264, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651145, Length=264, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXA_AZOC5 (P26482)

Other databases:

- EMBL:   X55450
- EMBL:   AP009384
- EMBL:   M35122
- PIR:   S14070
- RefSeq:   YP_001526363.1
- ProteinModelPortal:   P26482
- SMR:   P26482
- GeneID:   5688085
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3447
- HOGENOM:   HBG716515
- OMA:   YDLFSLE
- ProtClustDB:   CLSK2324433
- BioCyc:   ACAU438753:AZC_3447-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR21294
- PIRSF:   PIRSF000090
- SMART:   SM00893

Pfam domain/function: PF01012 ETF

EC number: NA

Molecular weight: Translated: 30111; Mature: 30111

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALALRDQHGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEE
MGPPSAEDSLRKALTFGADRAVLLTDRFFAGSDTLATTYALATAVRKIGETFGAPDIVFT
ECCCCHHHHHHHHHHCCCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GKQTIDGDTAQVGPGIAKRLGLLQLTYVAKIANVDLAGRSIQVERRSEGGVQVLQTRLPC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHH
LITMLEATNEIRRGAMADALRAARAEVVIWSAKDAGVEDISKCGLRGSPTIVKRVFAPSA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHCCCC
RAEKATMVDFSETAPADALIAEIFKRQPKLEDDLAALARGY
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALALRDQHGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEE
MGPPSAEDSLRKALTFGADRAVLLTDRFFAGSDTLATTYALATAVRKIGETFGAPDIVFT
ECCCCHHHHHHHHHHCCCCCEEEEEECEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GKQTIDGDTAQVGPGIAKRLGLLQLTYVAKIANVDLAGRSIQVERRSEGGVQVLQTRLPC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHH
LITMLEATNEIRRGAMADALRAARAEVVIWSAKDAGVEDISKCGLRGSPTIVKRVFAPSA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHCCCC
RAEKATMVDFSETAPADALIAEIFKRQPKLEDDLAALARGY
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088; 3216855