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The map label for this gene is nifV [H]

Identifier: 158425013

GI number: 158425013

Start: 3920412

End: 3921632

Strand: Reverse

Name: nifV [H]

Synonym: AZC_3389

Alternate gene names: 158425013

Gene position: 3921632-3920412 (Counterclockwise)

Preceding gene: 158425014

Following gene: 158425012

Centisome position: 73.03

GC content: 69.53

Gene sequence:

>1221_bases
GTGTTCCGTGGGAGGCCTGCCATGCTCGCCAAGACACCCGCAAACCCCGCGCCGCTTCAGCGGACGGCGTTCCTGAACGA
CACCACGCTGCGCGACGGCGAGCAGGCGCCGGGTGTCGCCTTCACCCGCAAGGAGAAGATCGAGATCGCCGCCGCCCTTG
CCGCCGCCGGTGTCCCGGAGATCGAGGCGGGAACGCCCGCCATGGGCGACGAAGAGGTGGAAACCATCCGCTCCATCGTC
TCGCTGAACCTCCCGACGCGCGTCATGGCCTGGTGCCGCATGAGCGAGGACGACCTGATGGCCGCCGTCGCGGCGGGCGT
GAAGATCGTCAATGTCTCCATTCCCACCTCCGACCGGCAACTGGCCGGCAAGCTCGGCAAGGATCGCGCCTGGGCGCTCG
GCCGTGTGGCGGAGGTGGTGACACTGGCGCGTCGGCTCGGCTTTGAGGTGGCGGTAGGGGGCGAGGATTCCTCGCGGGCC
GATCCCGATTTTCTCTGCCGTCTCGCGGAGACGGCGAAGGCGGCGGGCGCCTTTCGCCTGCGGCTGGCCGACACGCTTGG
CGTGCTTGACCCCTTCGGCACCTATGCATTGGTGCGCCGGGTGGCCGCCACCACCGACATCGAGCTTGAGTTCCACGCCC
ATGACGATCTCGGCCTTGCCACCGCCAATACGCTGGCGGCGGTGATGGGCGGAGCGCGTCACGCCAGCGTCACCGTCGCC
GGGCTCGGCGAGCGCGCGGGCAATGCCGCGCTGGAGGAAGTGGCCATCGCCCTGCGCCAGACGGCGCGGGCGGAGACCGG
CATCGCTCCGGCCGCGCTGAAGCCGCTGGCCGAACTAGTGTGCGGCGCCGCCGCCCGTCCGGTGCCGCGCGGCAAGGCCA
TCGTCGGCGCGGATGTGTTCACCCACGAGTCGGGCATCCATGTCTCCGGCCTGCTCAAGGACCGGGCCACCTATGAAGCT
CTGAATCCGGAACTGTTCGGGCGTGGCCACACGGTGGTGCTCGGAAAGCATTCCGGTCTTGCGGCGGTGGAGAAGGCGCT
GGCCGACGAGGGCATCACCGTGGATGCGGTGCGCGGGCGCGCCATTCTCGACCGGGTGCGGGCTTTTGCTGTCCGCACCA
AGGAGAATGTTTCCCGCGAGACGCTGCTGCGCTTCTATCAGGACAGCTTCACCGAGTCCGCGCTGCGTCTGCGGCGGGCC
GCCGTGGAAGGCGCAATCTGA

Upstream 100 bases:

>100_bases
GGCGTCCCCGCTCCCGGCCAGTACGGATGCTGATAAGCGGATTTAAAACAATGACTTAAGTGTTTGGCACAGCCGTTGCT
ACATCCCACGTGACCTCATT

Downstream 100 bases:

>100_bases
CGCGCATCCGGGTTCGGGATCGATCATCAAGCCGGCGGAACAGCCGGCGGACACTAGGCCGGCCCTGCCGGCCGGACCGG
TTGCGGAGAGGACGAGATGA

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFRGRPAMLAKTPANPAPLQRTAFLNDTTLRDGEQAPGVAFTRKEKIEIAAALAAAGVPEIEAGTPAMGDEEVETIRSIV
SLNLPTRVMAWCRMSEDDLMAAVAAGVKIVNVSIPTSDRQLAGKLGKDRAWALGRVAEVVTLARRLGFEVAVGGEDSSRA
DPDFLCRLAETAKAAGAFRLRLADTLGVLDPFGTYALVRRVAATTDIELEFHAHDDLGLATANTLAAVMGGARHASVTVA
GLGERAGNAALEEVAIALRQTARAETGIAPAALKPLAELVCGAAARPVPRGKAIVGADVFTHESGIHVSGLLKDRATYEA
LNPELFGRGHTVVLGKHSGLAAVEKALADEGITVDAVRGRAILDRVRAFAVRTKENVSRETLLRFYQDSFTESALRLRRA
AVEGAI

Sequences:

>Translated_406_residues
MFRGRPAMLAKTPANPAPLQRTAFLNDTTLRDGEQAPGVAFTRKEKIEIAAALAAAGVPEIEAGTPAMGDEEVETIRSIV
SLNLPTRVMAWCRMSEDDLMAAVAAGVKIVNVSIPTSDRQLAGKLGKDRAWALGRVAEVVTLARRLGFEVAVGGEDSSRA
DPDFLCRLAETAKAAGAFRLRLADTLGVLDPFGTYALVRRVAATTDIELEFHAHDDLGLATANTLAAVMGGARHASVTVA
GLGERAGNAALEEVAIALRQTARAETGIAPAALKPLAELVCGAAARPVPRGKAIVGADVFTHESGIHVSGLLKDRATYEA
LNPELFGRGHTVVLGKHSGLAAVEKALADEGITVDAVRGRAILDRVRAFAVRTKENVSRETLLRFYQDSFTESALRLRRA
AVEGAI
>Mature_406_residues
MFRGRPAMLAKTPANPAPLQRTAFLNDTTLRDGEQAPGVAFTRKEKIEIAAALAAAGVPEIEAGTPAMGDEEVETIRSIV
SLNLPTRVMAWCRMSEDDLMAAVAAGVKIVNVSIPTSDRQLAGKLGKDRAWALGRVAEVVTLARRLGFEVAVGGEDSSRA
DPDFLCRLAETAKAAGAFRLRLADTLGVLDPFGTYALVRRVAATTDIELEFHAHDDLGLATANTLAAVMGGARHASVTVA
GLGERAGNAALEEVAIALRQTARAETGIAPAALKPLAELVCGAAARPVPRGKAIVGADVFTHESGIHVSGLLKDRATYEA
LNPELFGRGHTVVLGKHSGLAAVEKALADEGITVDAVRGRAILDRVRAFAVRTKENVSRETLLRFYQDSFTESALRLRRA
AVEGAI

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=346, Percent_Identity=35.5491329479769, Blast_Score=175, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320071, Length=320, Percent_Identity=32.8125, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=331, Percent_Identity=32.02416918429, Blast_Score=163, Evalue=4e-41,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 42821; Mature: 42821

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGRPAMLAKTPANPAPLQRTAFLNDTTLRDGEQAPGVAFTRKEKIEIAAALAAAGVPE
CCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
IEAGTPAMGDEEVETIRSIVSLNLPTRVMAWCRMSEDDLMAAVAAGVKIVNVSIPTSDRQ
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCHH
LAGKLGKDRAWALGRVAEVVTLARRLGFEVAVGGEDSSRADPDFLCRLAETAKAAGAFRL
HHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
RLADTLGVLDPFGTYALVRRVAATTDIELEFHAHDDLGLATANTLAAVMGGARHASVTVA
EEHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEE
GLGERAGNAALEEVAIALRQTARAETGIAPAALKPLAELVCGAAARPVPRGKAIVGADVF
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECHHE
THESGIHVSGLLKDRATYEALNPELFGRGHTVVLGKHSGLAAVEKALADEGITVDAVRGR
ECCCCCEEEHHHHHHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEHHCCH
AILDRVRAFAVRTKENVSRETLLRFYQDSFTESALRLRRAAVEGAI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFRGRPAMLAKTPANPAPLQRTAFLNDTTLRDGEQAPGVAFTRKEKIEIAAALAAAGVPE
CCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCC
IEAGTPAMGDEEVETIRSIVSLNLPTRVMAWCRMSEDDLMAAVAAGVKIVNVSIPTSDRQ
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCHH
LAGKLGKDRAWALGRVAEVVTLARRLGFEVAVGGEDSSRADPDFLCRLAETAKAAGAFRL
HHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
RLADTLGVLDPFGTYALVRRVAATTDIELEFHAHDDLGLATANTLAAVMGGARHASVTVA
EEHHHHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEE
GLGERAGNAALEEVAIALRQTARAETGIAPAALKPLAELVCGAAARPVPRGKAIVGADVF
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECHHE
THESGIHVSGLLKDRATYEALNPELFGRGHTVVLGKHSGLAAVEKALADEGITVDAVRGR
ECCCCCEEEHHHHHHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEHHCCH
AILDRVRAFAVRTKENVSRETLLRFYQDSFTESALRLRRAAVEGAI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]