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The map label for this gene is gatA [H]

Identifier: 158424936

GI number: 158424936

Start: 3833594

End: 3835090

Strand: Reverse

Name: gatA [H]

Synonym: AZC_3312

Alternate gene names: 158424936

Gene position: 3835090-3833594 (Counterclockwise)

Preceding gene: 158424937

Following gene: 158424935

Centisome position: 71.42

GC content: 66.6

Gene sequence:

>1497_bases
ATGATTGATCGCAGAACGTTCATCAAGGCGGCGGGAACGGCCTTCGTCGCGTCAAGGCTCGCTACGACGGCCGCGCGGGC
CGAGGCGAACGATCCGCTCAGCTACGCCTCGGTTGACGAACTCGTCGCCCTGTTCCGGGCCCGGAAGGCTTCTCCGGTCG
AACTGCTGAAGGCGCAGATCAAGCGCATCGAGGCGTTCAACTCCAAGATAAATTGCATTACCGACGAACATTTCGACCAG
GCGCTGAAGGAAGCCCGGCAGTCCGAGGACCGGTATGGGCGCGGCGATGCAAGACCTCTCGAGGGCATCACGGTCGCGAT
CAAGGACGAGTTCAGCCGCCCTGGCTGGCGGGTAACGCAGGGCTCGCTCGTTTACAAAGAATCGCCGGCGATGACCGAAA
ACGACGCGATCATCGACGCGCTCGAAGCGGCCGGGGCCGTCATGCCGTTCCAGACGACGGTGCCGGAGTTCTATCTTTTT
ATCGGCGCCTCGACCCGCGCCTGGGGCACGACCCACAATCCGTGGAACCTCGCCTACTCTCCGGGCGGGTCGTCGGCGGG
CTCGGGGGCGGCGCTGGCTGCGGGCTTCGCAACGCTGGCGATGGGGTCGGACATGGGCGGCTCGATCCGCATCCCCGCCT
CGCAGAATGGCCTCTATGGGTTCAGGCCGCCGTTCGGGCGTGTCGCGAGCGGGGAAGTCCCCTTCTCGACCTCGGGCCCG
CTCGCCCGCCGCTGCGACGATCTCCTCCACCTCCAGAACGCGATCGTCGGCCCTTCCGACAGGGTCATGGCCGCCATCCG
GCCTCGCCTCGACTATCCCGCCCAGTATCCCGACCTCTCGGGATGGCGGATCGCCGTGGACTGGGGCGCGGCCGTCGCCG
AGGTCATTCCATCGGTGAAGGCGGCGATGCAGGACGGCGTGGACACGCTCCGCAAGGCGGGCTGCGTCGTGGACGAGGTC
GACTGCGGCTTCTCGAAGGATCAGAAACTCGTCTTCATTCGCGGCCTGATGGCGACGTCCCTGGGCACCCTGGTCGATGT
CGCGAATGCCAATCGCGGTCTGCTCTCGCCCTATATGGCGGAGGTGATCGACCTCGTCGGAGCCGTCGGACCGCGTCAGG
CGGAGGAAGCCGAGGCACTGGCCGAGCGCCTGCACCGGCAGGTTCAGCAGCGGGTCTTCGGCAAGGGGTACCGTGTCCTG
CTGATGCCCACCATGGCGACGCCGCTCGTCCGGGCGGATATGTTCAAGAGCAAGGCGGACAACGTCGACCTGTGGACGGG
CACGGGGTTCGGGGCGGCACTGACCTGGCCGTGGAACCTCCTCAACCGCTACCCGGTGGTGGACGTTCCGCTCGGCCTGG
TGGATGAGGGAATGCCCTCGGGCATGCAGGTGATCGGACAGACCTTCGCCGATCTCGACACCTTCCAGTTCGCGTCGACA
TGGGCGCGCCTGCGGCCGGCCTTGTTCGCCGATGGGCGGTTCCCGACCTTCGTCTGA

Upstream 100 bases:

>100_bases
ATCTACCTTTGACAAAGATGGTTGGTATTGGCCTTTGCGTCTTCGTGGCCGCCGCCCCGGTCTGCCTCGTGTTCGGACCC
TTTGACGAAAGGTTGCCCAC

Downstream 100 bases:

>100_bases
CGCGAGGGCGAGGACATGGCCCCGCGGGGGCCGATCTCCGCGCGCTCGACCTTCGGCGCCGCTGGAGCCTTCCGCCGGAT
GTTCTTCCCGCGGAAGGCGA

Product: twin-arginine translocation pathway signal precursor

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MIDRRTFIKAAGTAFVASRLATTAARAEANDPLSYASVDELVALFRARKASPVELLKAQIKRIEAFNSKINCITDEHFDQ
ALKEARQSEDRYGRGDARPLEGITVAIKDEFSRPGWRVTQGSLVYKESPAMTENDAIIDALEAAGAVMPFQTTVPEFYLF
IGASTRAWGTTHNPWNLAYSPGGSSAGSGAALAAGFATLAMGSDMGGSIRIPASQNGLYGFRPPFGRVASGEVPFSTSGP
LARRCDDLLHLQNAIVGPSDRVMAAIRPRLDYPAQYPDLSGWRIAVDWGAAVAEVIPSVKAAMQDGVDTLRKAGCVVDEV
DCGFSKDQKLVFIRGLMATSLGTLVDVANANRGLLSPYMAEVIDLVGAVGPRQAEEAEALAERLHRQVQQRVFGKGYRVL
LMPTMATPLVRADMFKSKADNVDLWTGTGFGAALTWPWNLLNRYPVVDVPLGLVDEGMPSGMQVIGQTFADLDTFQFAST
WARLRPALFADGRFPTFV

Sequences:

>Translated_498_residues
MIDRRTFIKAAGTAFVASRLATTAARAEANDPLSYASVDELVALFRARKASPVELLKAQIKRIEAFNSKINCITDEHFDQ
ALKEARQSEDRYGRGDARPLEGITVAIKDEFSRPGWRVTQGSLVYKESPAMTENDAIIDALEAAGAVMPFQTTVPEFYLF
IGASTRAWGTTHNPWNLAYSPGGSSAGSGAALAAGFATLAMGSDMGGSIRIPASQNGLYGFRPPFGRVASGEVPFSTSGP
LARRCDDLLHLQNAIVGPSDRVMAAIRPRLDYPAQYPDLSGWRIAVDWGAAVAEVIPSVKAAMQDGVDTLRKAGCVVDEV
DCGFSKDQKLVFIRGLMATSLGTLVDVANANRGLLSPYMAEVIDLVGAVGPRQAEEAEALAERLHRQVQQRVFGKGYRVL
LMPTMATPLVRADMFKSKADNVDLWTGTGFGAALTWPWNLLNRYPVVDVPLGLVDEGMPSGMQVIGQTFADLDTFQFAST
WARLRPALFADGRFPTFV
>Mature_498_residues
MIDRRTFIKAAGTAFVASRLATTAARAEANDPLSYASVDELVALFRARKASPVELLKAQIKRIEAFNSKINCITDEHFDQ
ALKEARQSEDRYGRGDARPLEGITVAIKDEFSRPGWRVTQGSLVYKESPAMTENDAIIDALEAAGAVMPFQTTVPEFYLF
IGASTRAWGTTHNPWNLAYSPGGSSAGSGAALAAGFATLAMGSDMGGSIRIPASQNGLYGFRPPFGRVASGEVPFSTSGP
LARRCDDLLHLQNAIVGPSDRVMAAIRPRLDYPAQYPDLSGWRIAVDWGAAVAEVIPSVKAAMQDGVDTLRKAGCVVDEV
DCGFSKDQKLVFIRGLMATSLGTLVDVANANRGLLSPYMAEVIDLVGAVGPRQAEEAEALAERLHRQVQQRVFGKGYRVL
LMPTMATPLVRADMFKSKADNVDLWTGTGFGAALTWPWNLLNRYPVVDVPLGLVDEGMPSGMQVIGQTFADLDTFQFAST
WARLRPALFADGRFPTFV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=516, Percent_Identity=25.3875968992248, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=25.9183673469388, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=27.3267326732673, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=292, Percent_Identity=30.1369863013699, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=225, Percent_Identity=32, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=298, Percent_Identity=30.2013422818792, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=194, Percent_Identity=36.0824742268041, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=132, Percent_Identity=34.0909090909091, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=169, Percent_Identity=31.3609467455621, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=158, Percent_Identity=32.9113924050633, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=527, Percent_Identity=24.2884250474383, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=464, Percent_Identity=23.0603448275862, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644968, Length=495, Percent_Identity=25.6565656565657, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356731, Length=474, Percent_Identity=27.4261603375527, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=26.9461077844311, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=31.0483870967742, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=31.0483870967742, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=31.0483870967742, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=304, Percent_Identity=27.9605263157895, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922090, Length=304, Percent_Identity=27.9605263157895, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652981, Length=304, Percent_Identity=27.9605263157895, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652983, Length=304, Percent_Identity=27.9605263157895, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648441, Length=181, Percent_Identity=31.4917127071823, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=181, Percent_Identity=31.4917127071823, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=225, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53804; Mature: 53804

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRRTFIKAAGTAFVASRLATTAARAEANDPLSYASVDELVALFRARKASPVELLKAQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KRIEAFNSKINCITDEHFDQALKEARQSEDRYGRGDARPLEGITVAIKDEFSRPGWRVTQ
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEC
GSLVYKESPAMTENDAIIDALEAAGAVMPFQTTVPEFYLFIGASTRAWGTTHNPWNLAYS
CCEEEECCCCCCCCHHHHHHHHHCCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCEEEEC
PGGSSAGSGAALAAGFATLAMGSDMGGSIRIPASQNGLYGFRPPFGRVASGEVPFSTSGP
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LARRCDDLLHLQNAIVGPSDRVMAAIRPRLDYPAQYPDLSGWRIAVDWGAAVAEVIPSVK
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AAMQDGVDTLRKAGCVVDEVDCGFSKDQKLVFIRGLMATSLGTLVDVANANRGLLSPYMA
HHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHH
EVIDLVGAVGPRQAEEAEALAERLHRQVQQRVFGKGYRVLLMPTMATPLVRADMFKSKAD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCC
NVDLWTGTGFGAALTWPWNLLNRYPVVDVPLGLVDEGMPSGMQVIGQTFADLDTFQFAST
CCEEEECCCCCCEEECCHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
WARLRPALFADGRFPTFV
HHHHCHHHEECCCCCCCC
>Mature Secondary Structure
MIDRRTFIKAAGTAFVASRLATTAARAEANDPLSYASVDELVALFRARKASPVELLKAQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KRIEAFNSKINCITDEHFDQALKEARQSEDRYGRGDARPLEGITVAIKDEFSRPGWRVTQ
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEC
GSLVYKESPAMTENDAIIDALEAAGAVMPFQTTVPEFYLFIGASTRAWGTTHNPWNLAYS
CCEEEECCCCCCCCHHHHHHHHHCCCCCCCHHCCCEEEEEEECCCCCCCCCCCCCEEEEC
PGGSSAGSGAALAAGFATLAMGSDMGGSIRIPASQNGLYGFRPPFGRVASGEVPFSTSGP
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LARRCDDLLHLQNAIVGPSDRVMAAIRPRLDYPAQYPDLSGWRIAVDWGAAVAEVIPSVK
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AAMQDGVDTLRKAGCVVDEVDCGFSKDQKLVFIRGLMATSLGTLVDVANANRGLLSPYMA
HHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHH
EVIDLVGAVGPRQAEEAEALAERLHRQVQQRVFGKGYRVLLMPTMATPLVRADMFKSKAD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCC
NVDLWTGTGFGAALTWPWNLLNRYPVVDVPLGLVDEGMPSGMQVIGQTFADLDTFQFAST
CCEEEECCCCCCEEECCHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
WARLRPALFADGRFPTFV
HHHHCHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA