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The map label for this gene is mdeA [H]

Identifier: 158424867

GI number: 158424867

Start: 3739503

End: 3740672

Strand: Reverse

Name: mdeA [H]

Synonym: AZC_3243

Alternate gene names: 158424867

Gene position: 3740672-3739503 (Counterclockwise)

Preceding gene: 158424868

Following gene: 158424861

Centisome position: 69.66

GC content: 67.01

Gene sequence:

>1170_bases
ATGAACAAGCCGCTCGATCACGAGGCCCTGCCGGCTCAGGGGGGCTGGAGCCGCGAGGTCAATCTGCTGGCGGCGGCGGT
GCCGCCGATCTTCCAGACCTCGCTGTTCACCTTCGACACCTATGCCGACATGGCCGCGGTCTATGCCGGGCGCTCGCATC
AGCTGATCTATTCGCGGGGCGACAATCCGACGGTGATGGCGTTCGAGCGGGCGGTGGCCGAGCTTGAGGGCGCGGAAGCT
GGACGGGCCTTCTCCAGCGGCATGGGGGCCATCAGCGCCACCGTGCTCGCGCTTGTTTCGGCCGGTGACCGCATCGTCAC
CGTGCGCAACGTCTACAGCGACGCCTATCGTCTGTTCGAGCTGATGCTCTCGCGCTTCGGCGTCGTGGTGGACTATGTGG
ACGGCACGGATGCGGAGGCGGTCGAGGCCGTGCTGCCCGGTGCCAAGCTGCTCTATCTCGAGAGCCCAACCTCCCTGGCG
TTCGAGCTTCAGGACATCGCGCGCCTCGCGCGGGCGGCCCGAACGCACGGTGTCGTGAGCGTGATCGACAACAGCTGGGC
GACACCGCTCTTCCAGCGCCCCATCGCCCATGGTGTCGATCTGGTGGTCCATGCGGCCTCCAAATATCTCGGAGGTCATA
GCGACACGGTGGCCGGTGTGGTCGTGGGCTCGGCCGAACTGGTCGGCCGGATCAACGCATCAAGCTATGCCTATCTTGGA
GCCAAGCTCTCGCCCTTCGAGGCATGGTTGCTGCTGCGCGGCCTGCACACCCTGCCCATGCGCATGGCGCGTCACATGTC
GAGTGGCCTTGCCCTTGCCGAACGTCTGCGGGCGCATCCCGACGTGGAACTGGTCCGGCATCCGGCGTTCAGCGATCACC
CCGGCCGCGCGAGTCTCAGCGGTTTCGCAGGCGTGTTCTCGTTCGAGGTTGGCGAGGCCATCGATGTCCCGACCTTTGTC
GACGCCTTGCGGCTGGTGCGCATCGGCGTCAGCTGGGGCGGGCCGGAAAGCCTCGTCGTGCCGGCCCTCGCCCCGCTTCA
GCTGGCCAAGGCCAATTGCTTCGCGCGGTTCGGCATCAGCCCGCGCCTGATCCGGCTCAGCGCCGGCATGGAGGATGAGA
CGCTGATCTGGGCCGATATCGAGCAGGCGCTCCATGGCGCGCGTCGCTGA

Upstream 100 bases:

>100_bases
TGTCCGCCGCCCTTCTCCTGCGCGAAGTGCGGCGCCTCCCGACCGCCCGGTCGCGAAAGTCATCCCCATCACTGGCCGCT
GAAGGCGCTGGAGGTTCCGC

Downstream 100 bases:

>100_bases
TCGGCCCACGGCTGGCGGCGCTCCCGCCCTCAGGCGGCCGTCCGCCGGTCGATCTTGAGAGAGAGACGGACCGGGGCGGG
CTTTGCCCCGTTCCTGCCGT

Product: hypothetical protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNKPLDHEALPAQGGWSREVNLLAAAVPPIFQTSLFTFDTYADMAAVYAGRSHQLIYSRGDNPTVMAFERAVAELEGAEA
GRAFSSGMGAISATVLALVSAGDRIVTVRNVYSDAYRLFELMLSRFGVVVDYVDGTDAEAVEAVLPGAKLLYLESPTSLA
FELQDIARLARAARTHGVVSVIDNSWATPLFQRPIAHGVDLVVHAASKYLGGHSDTVAGVVVGSAELVGRINASSYAYLG
AKLSPFEAWLLLRGLHTLPMRMARHMSSGLALAERLRAHPDVELVRHPAFSDHPGRASLSGFAGVFSFEVGEAIDVPTFV
DALRLVRIGVSWGGPESLVVPALAPLQLAKANCFARFGISPRLIRLSAGMEDETLIWADIEQALHGARR

Sequences:

>Translated_389_residues
MNKPLDHEALPAQGGWSREVNLLAAAVPPIFQTSLFTFDTYADMAAVYAGRSHQLIYSRGDNPTVMAFERAVAELEGAEA
GRAFSSGMGAISATVLALVSAGDRIVTVRNVYSDAYRLFELMLSRFGVVVDYVDGTDAEAVEAVLPGAKLLYLESPTSLA
FELQDIARLARAARTHGVVSVIDNSWATPLFQRPIAHGVDLVVHAASKYLGGHSDTVAGVVVGSAELVGRINASSYAYLG
AKLSPFEAWLLLRGLHTLPMRMARHMSSGLALAERLRAHPDVELVRHPAFSDHPGRASLSGFAGVFSFEVGEAIDVPTFV
DALRLVRIGVSWGGPESLVVPALAPLQLAKANCFARFGISPRLIRLSAGMEDETLIWADIEQALHGARR
>Mature_389_residues
MNKPLDHEALPAQGGWSREVNLLAAAVPPIFQTSLFTFDTYADMAAVYAGRSHQLIYSRGDNPTVMAFERAVAELEGAEA
GRAFSSGMGAISATVLALVSAGDRIVTVRNVYSDAYRLFELMLSRFGVVVDYVDGTDAEAVEAVLPGAKLLYLESPTSLA
FELQDIARLARAARTHGVVSVIDNSWATPLFQRPIAHGVDLVVHAASKYLGGHSDTVAGVVVGSAELVGRINASSYAYLG
AKLSPFEAWLLLRGLHTLPMRMARHMSSGLALAERLRAHPDVELVRHPAFSDHPGRASLSGFAGVFSFEVGEAIDVPTFV
DALRLVRIGVSWGGPESLVVPALAPLQLAKANCFARFGISPRLIRLSAGMEDETLIWADIEQALHGARR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=373, Percent_Identity=36.7292225201072, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI299473759, Length=373, Percent_Identity=33.2439678284182, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=32.8840970350404, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1790375, Length=372, Percent_Identity=34.9462365591398, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=30.1020408163265, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=369, Percent_Identity=35.5013550135501, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=32.3450134770889, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=372, Percent_Identity=34.9462365591398, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=315, Percent_Identity=34.2857142857143, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=32.9015544041451, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=415, Percent_Identity=27.9518072289157, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=27.1393643031785, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=377, Percent_Identity=24.6684350132626, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=34.9206349206349, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 41715; Mature: 41715

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPLDHEALPAQGGWSREVNLLAAAVPPIFQTSLFTFDTYADMAAVYAGRSHQLIYSRG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DNPTVMAFERAVAELEGAEAGRAFSSGMGAISATVLALVSAGDRIVTVRNVYSDAYRLFE
CCCEEHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
LMLSRFGVVVDYVDGTDAEAVEAVLPGAKLLYLESPTSLAFELQDIARLARAARTHGVVS
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VIDNSWATPLFQRPIAHGVDLVVHAASKYLGGHSDTVAGVVVGSAELVGRINASSYAYLG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHEECCCCCEEEEC
AKLSPFEAWLLLRGLHTLPMRMARHMSSGLALAERLRAHPDVELVRHPAFSDHPGRASLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
GFAGVFSFEVGEAIDVPTFVDALRLVRIGVSWGGPESLVVPALAPLQLAKANCFARFGIS
HHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHEECCCC
PRLIRLSAGMEDETLIWADIEQALHGARR
CEEEEEECCCCCCEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MNKPLDHEALPAQGGWSREVNLLAAAVPPIFQTSLFTFDTYADMAAVYAGRSHQLIYSRG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DNPTVMAFERAVAELEGAEAGRAFSSGMGAISATVLALVSAGDRIVTVRNVYSDAYRLFE
CCCEEHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
LMLSRFGVVVDYVDGTDAEAVEAVLPGAKLLYLESPTSLAFELQDIARLARAARTHGVVS
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VIDNSWATPLFQRPIAHGVDLVVHAASKYLGGHSDTVAGVVVGSAELVGRINASSYAYLG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHEECCCCCEEEEC
AKLSPFEAWLLLRGLHTLPMRMARHMSSGLALAERLRAHPDVELVRHPAFSDHPGRASLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCC
GFAGVFSFEVGEAIDVPTFVDALRLVRIGVSWGGPESLVVPALAPLQLAKANCFARFGIS
HHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHEECCCC
PRLIRLSAGMEDETLIWADIEQALHGARR
CEEEEEECCCCCCEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]