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The map label for this gene is fadE25 [H]

Identifier: 158424811

GI number: 158424811

Start: 3644683

End: 3645864

Strand: Reverse

Name: fadE25 [H]

Synonym: AZC_3187

Alternate gene names: 158424811

Gene position: 3645864-3644683 (Counterclockwise)

Preceding gene: 158424812

Following gene: 158424810

Centisome position: 67.9

GC content: 70.47

Gene sequence:

>1182_bases
ATGAGCACCCCCGCAGCCGCAGGCCCGACTCGTGAACCGCTGGACTGGCCCTTCTTCGGCCCGGAGCATCGCGCCTATGC
CGCGCGCCTCGATGGGTTCGTCGCCGGGGGCGGGCTGGACGGGCTCGATCATGCGGACGTGGACGGCACCTGCCGCACTC
TGGTGGCGCGCCTCGGTGCCACCGGTCTGCTGGAAGCGGCGGTACCAACGCCCGAGGCCGACGGCACCTCCATCCGCTCG
CTGGAACTCTGCCTCGCCCGCGAGACGCTGGCTTGGCACGACGGGTTGGCGGATTTCGCCTTCGCCATGCAGGGGCTGGG
CACGGGGGCCATCGCGCTCGCCGGCTCGGATGCCTTGAAGCGCGAGGTGCTGCCGAAGGTGCGCCGGGGTGAATGGCTGG
CCGCCTTCGCGCTCTCCGAGGCGGATGCCGGCTCCGATGTGGCCGCCATGAGCTGCGCCGCCCGCGCCGATGGCGACCAT
TATGTGCTGGATGGCGAGAAGACCTGGATTTCCAACGGCGGCATTGCTGACGTCTACACGGTTTTCGTGCGCACGGGGGA
GGCACCGGGCACGCGTGGCATCTCGGCTTTCGTCGTCTTCGCGGATGATCCCGGCTTCTCCATCGTGGAACGGATCGACG
TGATCGCGCCGCACCCGCTGGCGACGATCCGCTTCGATGGCTGCCGCATTCCGGCCTCCCGCCGGCTCGGCGCGGCCGGA
GAGGGCTTCAAGATTGCCATGCGGACGCTGGATATCTTCCGCGCCTCCGTTGCGGCAGCAGCGCTCGGCTTCGCGCGGCG
GGCGTTCGACGAGGCGCTCCACCACGTCCGAAGCAGGCGGATGCTGGGCGGCACCCTCGCCGACCTGCAACTCACGCAGG
CCGCCCTCGGCGATATGGCGGCGGAGCTGGATGGGGCGGCGCTGCTGACATGGCGCGCCGCCTGGCGCCGGGATGTGCAG
GGCCTGCCGACCACCCGCGAGGCCGCGATGGCCAAGATGACCGCCACCGAAATGGCCCAGCGGGTGATCGACCGCGCCGT
CCAGCTTCATGGCGGCCGGGGCGTGCGGTCCGGCGAAACTGTCGAGGCGCTCTATCGCGAGATCCGCGCGCTGCGGATCT
ATGAGGGCGCGACGGAGGTGCAGAAGCTCATCATCGCCCGCGAACTCCTGAAGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGCAGGCGCAGGCCATCTGCATGGCGACGCAGGACTTCCGCCGCGCCTTCGAGGCTTTTGCCGCCAAGGCGAAACCCGCT
TTCGAGGGGAACTGAGCCCG

Downstream 100 bases:

>100_bases
GGCGGCGACATGGCTTTCGAGACCTCCCGCCTTCTGGGCTTCGGCGATTGTGATCCGGCCGGCATCGCCTTCTTTCCCGG
CTACATGCGTCTGCTCGTAA

Product: acyl-CoA dehydrogenase

Products: 2-butenoyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSTPAAAGPTREPLDWPFFGPEHRAYAARLDGFVAGGGLDGLDHADVDGTCRTLVARLGATGLLEAAVPTPEADGTSIRS
LELCLARETLAWHDGLADFAFAMQGLGTGAIALAGSDALKREVLPKVRRGEWLAAFALSEADAGSDVAAMSCAARADGDH
YVLDGEKTWISNGGIADVYTVFVRTGEAPGTRGISAFVVFADDPGFSIVERIDVIAPHPLATIRFDGCRIPASRRLGAAG
EGFKIAMRTLDIFRASVAAAALGFARRAFDEALHHVRSRRMLGGTLADLQLTQAALGDMAAELDGAALLTWRAAWRRDVQ
GLPTTREAAMAKMTATEMAQRVIDRAVQLHGGRGVRSGETVEALYREIRALRIYEGATEVQKLIIARELLKAG

Sequences:

>Translated_393_residues
MSTPAAAGPTREPLDWPFFGPEHRAYAARLDGFVAGGGLDGLDHADVDGTCRTLVARLGATGLLEAAVPTPEADGTSIRS
LELCLARETLAWHDGLADFAFAMQGLGTGAIALAGSDALKREVLPKVRRGEWLAAFALSEADAGSDVAAMSCAARADGDH
YVLDGEKTWISNGGIADVYTVFVRTGEAPGTRGISAFVVFADDPGFSIVERIDVIAPHPLATIRFDGCRIPASRRLGAAG
EGFKIAMRTLDIFRASVAAAALGFARRAFDEALHHVRSRRMLGGTLADLQLTQAALGDMAAELDGAALLTWRAAWRRDVQ
GLPTTREAAMAKMTATEMAQRVIDRAVQLHGGRGVRSGETVEALYREIRALRIYEGATEVQKLIIARELLKAG
>Mature_392_residues
STPAAAGPTREPLDWPFFGPEHRAYAARLDGFVAGGGLDGLDHADVDGTCRTLVARLGATGLLEAAVPTPEADGTSIRSL
ELCLARETLAWHDGLADFAFAMQGLGTGAIALAGSDALKREVLPKVRRGEWLAAFALSEADAGSDVAAMSCAARADGDHY
VLDGEKTWISNGGIADVYTVFVRTGEAPGTRGISAFVVFADDPGFSIVERIDVIAPHPLATIRFDGCRIPASRRLGAAGE
GFKIAMRTLDIFRASVAAAALGFARRAFDEALHHVRSRRMLGGTLADLQLTQAALGDMAAELDGAALLTWRAAWRRDVQG
LPTTREAAMAKMTATEMAQRVIDRAVQLHGGRGVRSGETVEALYREIRALRIYEGATEVQKLIIARELLKAG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=343, Percent_Identity=39.6501457725948, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI187960098, Length=340, Percent_Identity=33.8235294117647, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI4557231, Length=340, Percent_Identity=33.8235294117647, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI226958412, Length=351, Percent_Identity=30.4843304843305, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI226958414, Length=336, Percent_Identity=31.25, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI7656849, Length=284, Percent_Identity=34.1549295774648, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4501859, Length=341, Percent_Identity=32.8445747800587, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI76496475, Length=345, Percent_Identity=33.6231884057971, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4557235, Length=353, Percent_Identity=33.4277620396601, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21361497, Length=295, Percent_Identity=33.2203389830508, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4501857, Length=290, Percent_Identity=30.6896551724138, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI211971008, Length=288, Percent_Identity=31.9444444444444, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI48976061, Length=288, Percent_Identity=31.9444444444444, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI38505218, Length=303, Percent_Identity=27.0627062706271, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4503943, Length=352, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI7669494, Length=268, Percent_Identity=26.865671641791, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786223, Length=264, Percent_Identity=28.030303030303, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081958, Length=284, Percent_Identity=28.5211267605634, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082384, Length=287, Percent_Identity=26.1324041811847, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17570075, Length=343, Percent_Identity=32.6530612244898, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17569725, Length=344, Percent_Identity=31.6860465116279, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71985184, Length=344, Percent_Identity=35.4651162790698, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17533517, Length=279, Percent_Identity=34.7670250896057, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17534899, Length=345, Percent_Identity=31.5942028985507, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17508101, Length=279, Percent_Identity=35.4838709677419, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17538396, Length=343, Percent_Identity=32.0699708454811, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17506239, Length=275, Percent_Identity=33.0909090909091, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71990804, Length=224, Percent_Identity=35.2678571428571, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71985192, Length=290, Percent_Identity=33.7931034482759, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71982178, Length=222, Percent_Identity=31.981981981982, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17551932, Length=285, Percent_Identity=30.5263157894737, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17505929, Length=327, Percent_Identity=30.2752293577982, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32563615, Length=179, Percent_Identity=32.4022346368715, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531909, Length=240, Percent_Identity=27.5, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17534353, Length=339, Percent_Identity=26.8436578171091, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562446, Length=309, Percent_Identity=28.8025889967638, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI86563383, Length=305, Percent_Identity=25.9016393442623, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI86563381, Length=305, Percent_Identity=25.9016393442623, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646207, Length=339, Percent_Identity=37.7581120943953, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21356377, Length=342, Percent_Identity=37.1345029239766, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24660351, Length=345, Percent_Identity=34.2028985507246, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24666513, Length=279, Percent_Identity=35.4838709677419, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281363737, Length=353, Percent_Identity=32.0113314447592, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21355753, Length=353, Percent_Identity=29.1784702549575, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19920834, Length=344, Percent_Identity=26.453488372093, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.2

Molecular weight: Translated: 41721; Mature: 41590

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPAAAGPTREPLDWPFFGPEHRAYAARLDGFVAGGGLDGLDHADVDGTCRTLVARLGA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHCC
TGLLEAAVPTPEADGTSIRSLELCLARETLAWHDGLADFAFAMQGLGTGAIALAGSDALK
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHH
REVLPKVRRGEWLAAFALSEADAGSDVAAMSCAARADGDHYVLDGEKTWISNGGIADVYT
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCEEEECCCCHHHHHH
VFVRTGEAPGTRGISAFVVFADDPGFSIVERIDVIAPHPLATIRFDGCRIPASRRLGAAG
EEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCEECCHHCCCCCCC
EGFKIAMRTLDIFRASVAAAALGFARRAFDEALHHVRSRRMLGGTLADLQLTQAALGDMA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELDGAALLTWRAAWRRDVQGLPTTREAAMAKMTATEMAQRVIDRAVQLHGGRGVRSGET
HHCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VEALYREIRALRIYEGATEVQKLIIARELLKAG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STPAAAGPTREPLDWPFFGPEHRAYAARLDGFVAGGGLDGLDHADVDGTCRTLVARLGA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHCC
TGLLEAAVPTPEADGTSIRSLELCLARETLAWHDGLADFAFAMQGLGTGAIALAGSDALK
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHHHH
REVLPKVRRGEWLAAFALSEADAGSDVAAMSCAARADGDHYVLDGEKTWISNGGIADVYT
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCEEEECCCCHHHHHH
VFVRTGEAPGTRGISAFVVFADDPGFSIVERIDVIAPHPLATIRFDGCRIPASRRLGAAG
EEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCEECCHHCCCCCCC
EGFKIAMRTLDIFRASVAAAALGFARRAFDEALHHVRSRRMLGGTLADLQLTQAALGDMA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AELDGAALLTWRAAWRRDVQGLPTTREAAMAKMTATEMAQRVIDRAVQLHGGRGVRSGET
HHCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VEALYREIRALRIYEGATEVQKLIIARELLKAG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: butanoyl-CoA; acceptor

Specific reaction: butanoyl-CoA + acceptor = 2-butenoyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]