Click here to switch to the map view.

The map label for this gene is echA12 [H]

Identifier: 158424812

GI number: 158424812

Start: 3645870

End: 3646721

Strand: Reverse

Name: echA12 [H]

Synonym: AZC_3188

Alternate gene names: 158424812

Gene position: 3646721-3645870 (Counterclockwise)

Preceding gene: 158424813

Following gene: 158424811

Centisome position: 67.91

GC content: 68.43

Gene sequence:

>852_bases
ATGAGCGAGACCCGATCCACGATGGCGGCCATGAAGCGTCCGTTCAAGGACCACCGCGCCACCCATTTCAGCTTCGAGAC
GAGCGCCGATGGCCGTGTCGCGACCGTCCGCCTCAACCGGCCGGAGCGCAAGAACCCGCTGACCTTCGAGAGCTATGCCG
AGTTGCGCGACCTGTTCCGCGATCTCGTCTATGCCTCGGATGTGCGGGCCATCGTGCTCGCCGGGGCCGGGGGCAATTTC
TCCTCGGGCGGCGATGTGTTCGAGATCATCGAGCCGCTCACCCGCATGGCCATGCCCGATCTCCTTGCCTTCACGCGGAT
GACCGGCGATCTCGTGAAGGCCATGCGGCGCTGCCCGCAGCCCATCGTTGCCGCCGTCGACGGCGTCTGCGCCGGCGCCG
GCGCGATCCTCGCCATGGCCTCCGACCTGCGGCTGGCGACGCCCGAGGCCAAGACGGCCTTTCTCTTTGCCCGCGTGGGC
CTTGCCGGCGCTGACATGGGGGCGTGCGGGATCCTGCCGCGCGTCATCGGGCAGGGGCGGGCGGCTGAGCTGCTGTTCAC
CGGCCGCGCCATGACCGCCGCCGAGGGGGCGGCGTGGGGCTTCTACAACGCCCTCCATCCGGCCGCGGACATGGAGGCGA
AGGCGCAGGAACTCGCCCGCTCGCTGGCGGACGGCCCATGGTTCGCCCATGGCATGACCAAGACCATGCTAAATCAGGAA
TGGGCCATGGGCCTTGAAGAACTGATCGAGGCGGAAGCGCAGGCGCAGGCCATCTGCATGGCGACGCAGGACTTCCGCCG
CGCCTTCGAGGCTTTTGCCGCCAAGGCGAAACCCGCTTTCGAGGGGAACTGA

Upstream 100 bases:

>100_bases
CGGACGAGATGGCGGAAACGGTGGCCTGGGTGGTGTCGCCCGGAGCCGCGTCGATCAACGGACAGGCGATCGCGGTCGCC
GGTGGCGAGGTGATGGTGGG

Downstream 100 bases:

>100_bases
GCCCGATGAGCACCCCCGCAGCCGCAGGCCCGACTCGTGAACCGCTGGACTGGCCCTTCTTCGGCCCGGAGCATCGCGCC
TATGCCGCGCGCCTCGATGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSETRSTMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFRDLVYASDVRAIVLAGAGGNF
SSGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQPIVAAVDGVCAGAGAILAMASDLRLATPEAKTAFLFARVG
LAGADMGACGILPRVIGQGRAAELLFTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQE
WAMGLEELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN

Sequences:

>Translated_283_residues
MSETRSTMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFRDLVYASDVRAIVLAGAGGNF
SSGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQPIVAAVDGVCAGAGAILAMASDLRLATPEAKTAFLFARVG
LAGADMGACGILPRVIGQGRAAELLFTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQE
WAMGLEELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN
>Mature_282_residues
SETRSTMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFRDLVYASDVRAIVLAGAGGNFS
SGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQPIVAAVDGVCAGAGAILAMASDLRLATPEAKTAFLFARVGL
AGADMGACGILPRVIGQGRAAELLFTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQEW
AMGLEELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=25.5905511811024, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=197, Percent_Identity=29.9492385786802, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=33.7301587301587, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=28.030303030303, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=25.3787878787879, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=26.7441860465116, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=24.6212121212121, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=28.0788177339901, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=207, Percent_Identity=29.951690821256, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=28.755364806867, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=29.0909090909091, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.1338582677165, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=25.8687258687259, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=25.9842519685039, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=23.015873015873, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=195, Percent_Identity=28.2051282051282, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19920382, Length=256, Percent_Identity=30.078125, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357171, Length=197, Percent_Identity=30.9644670050761, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653139, Length=276, Percent_Identity=26.8115942028986, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=27.3764258555133, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=27.3764258555133, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=23.4375, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=22.4, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24650670, Length=198, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30461; Mature: 30330

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETRSTMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFR
CCHHHHHHHHHHCCHHHHCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
DLVYASDVRAIVLAGAGGNFSSGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQ
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIVAAVDGVCAGAGAILAMASDLRLATPEAKTAFLFARVGLAGADMGACGILPRVIGQGR
HHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
AAELLFTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQE
CEEEEEECCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
WAMGLEELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SETRSTMAAMKRPFKDHRATHFSFETSADGRVATVRLNRPERKNPLTFESYAELRDLFR
CHHHHHHHHHHCCHHHHCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
DLVYASDVRAIVLAGAGGNFSSGGDVFEIIEPLTRMAMPDLLAFTRMTGDLVKAMRRCPQ
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIVAAVDGVCAGAGAILAMASDLRLATPEAKTAFLFARVGLAGADMGACGILPRVIGQGR
HHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
AAELLFTGRAMTAAEGAAWGFYNALHPAADMEAKAQELARSLADGPWFAHGMTKTMLNQE
CEEEEEECCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
WAMGLEELIEAEAQAQAICMATQDFRRAFEAFAAKAKPAFEGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]