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The map label for this gene is ntrB [H]

Identifier: 158424711

GI number: 158424711

Start: 3533132

End: 3534256

Strand: Reverse

Name: ntrB [H]

Synonym: AZC_3087

Alternate gene names: 158424711

Gene position: 3534256-3533132 (Counterclockwise)

Preceding gene: 158424712

Following gene: 158424710

Centisome position: 65.82

GC content: 66.58

Gene sequence:

>1125_bases
ATGAAGGCGGAACTGAAGCCTCGCACCTCGGACGGCGAGGCCCTGACCCGCGCCATCCTGGATGCGGTGCCGCATCCGGT
GGTGACGCTGGCGGAAGACGGCCGCATTCTCGATGCCAATGTGGCCGCCGAGACCTTCTTTCAGGTCAGCGTGCCGCTGC
TGCGCCGCCGGCCTTTCGCGGAGTTCGTGCCCTTCGGCACGCCGCTGCTGAGCCTCATCGAGCAGGTGCGCGAGCGCGGT
TCCTCGGTGAACGAATATCGCGTCGATCTCGGCACGCCGCGCAATGGCGGTGAGCGCATCGTGGACATCCATGTGGCGCC
CATGGCCGAGCGGCCGGGCCATGTGGTGGTGATGTTCCAGGAGCGCACCATCGCCGAGAAGATGGACCGCCAGCTCACCC
ATCGCGGCGCTGCCCGTTCCGTGAGCGCGCTCGCTTCCATGCTGGCGCACGAGATCAAGAACCCGCTCTCCGGCATCCGC
GGCGCCGCCCAGCTTCTGGAGCAGTCGGCGGGCGACGAGGACCGGGCGCTCACACGCCTCATCTGCGACGAGGCGGACCG
CATCGTGAAGCTGGTGGACCGCATGGAGGTGTTCTCCGACGAGCGCCCCGTGGAGCGCGAGCCGGTCAATATCCATGCGG
TGCTTGAGCATGTGCGCACGCTCGCGTCGTCGGGCTTCGCGCGCCACATCCGCTTCATCGAGGATTACGATCCCTCTCTG
CCGCCGGTCTATGCCAACCGCGACCAGCTCATCCAGGTCTTCCTCAATCTGGTGAAGAATGCGGCAGAAGCCATCGACAG
CGAGACCGATGGCGAAATCCACCTCACCACCGCCTTCCGTCCCGGCGTGCGCCTCACCGTGCCCGGCGCCTCCTCCAAGG
TCAGCCTGCCGCTGGAGTTCTGCGTGCGGGACAATGGCAAGGGCGTGCCGGAGGATCTCGTCCAGCACCTCTTCGATCCC
TTCGTGACCACCAAGCCCACCGGCAGTGGCCTCGGCCTTGCATTGGTGGCGAAGATCATCGGCGACCATGGCGGTATCGT
CGAATGCGACAGCCAGCCCCGCCGCACCACGTTCCGCGTCCTGCTGCCCATGTACCGCAGCAGCCGGTCTTCAGAGGACA
ACTGA

Upstream 100 bases:

>100_bases
CCAAGGATGCGCGTGGCCGCATCCTCACTCTGGAGGCCCCCGACGCTGTGATCGCGGGCATCCGCACCTTTTACGACCAA
CTCGCCTGGAGGGCGGCCGC

Downstream 100 bases:

>100_bases
GGTGAGCATGCCTACCGGAAGCATCCTGGTCGCCGACGACGATGCCGCGATCCGCACCGTGCTCAATCAGGCCCTGTCGC
GCGCCGGCTATGAGGTGCGC

Product: nitrogen regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKAELKPRTSDGEALTRAILDAVPHPVVTLAEDGRILDANVAAETFFQVSVPLLRRRPFAEFVPFGTPLLSLIEQVRERG
SSVNEYRVDLGTPRNGGERIVDIHVAPMAERPGHVVVMFQERTIAEKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIR
GAAQLLEQSAGDEDRALTRLICDEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLASSGFARHIRFIEDYDPSL
PPVYANRDQLIQVFLNLVKNAAEAIDSETDGEIHLTTAFRPGVRLTVPGASSKVSLPLEFCVRDNGKGVPEDLVQHLFDP
FVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRSSRSSEDN

Sequences:

>Translated_374_residues
MKAELKPRTSDGEALTRAILDAVPHPVVTLAEDGRILDANVAAETFFQVSVPLLRRRPFAEFVPFGTPLLSLIEQVRERG
SSVNEYRVDLGTPRNGGERIVDIHVAPMAERPGHVVVMFQERTIAEKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIR
GAAQLLEQSAGDEDRALTRLICDEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLASSGFARHIRFIEDYDPSL
PPVYANRDQLIQVFLNLVKNAAEAIDSETDGEIHLTTAFRPGVRLTVPGASSKVSLPLEFCVRDNGKGVPEDLVQHLFDP
FVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRSSRSSEDN
>Mature_374_residues
MKAELKPRTSDGEALTRAILDAVPHPVVTLAEDGRILDANVAAETFFQVSVPLLRRRPFAEFVPFGTPLLSLIEQVRERG
SSVNEYRVDLGTPRNGGERIVDIHVAPMAERPGHVVVMFQERTIAEKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIR
GAAQLLEQSAGDEDRALTRLICDEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLASSGFARHIRFIEDYDPSL
PPVYANRDQLIQVFLNLVKNAAEAIDSETDGEIHLTTAFRPGVRLTVPGASSKVSLPLEFCVRDNGKGVPEDLVQHLFDP
FVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRSSRSSEDN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=355, Percent_Identity=34.3661971830986, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788549, Length=361, Percent_Identity=27.9778393351801, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=34.2222222222222, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI48994928, Length=388, Percent_Identity=23.7113402061856, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.1186440677966, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=26.5217391304348, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790567, Length=123, Percent_Identity=35.7723577235772, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41255; Mature: 41255

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAELKPRTSDGEALTRAILDAVPHPVVTLAEDGRILDANVAAETFFQVSVPLLRRRPFA
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHCCCHH
EFVPFGTPLLSLIEQVRERGSSVNEYRVDLGTPRNGGERIVDIHVAPMAERPGHVVVMFQ
HHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEEC
ERTIAEKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIRGAAQLLEQSAGDEDRALTRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ICDEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLASSGFARHIRFIEDYDPSL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PPVYANRDQLIQVFLNLVKNAAEAIDSETDGEIHLTTAFRPGVRLTVPGASSKVSLPLEF
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCEECCHHE
CVRDNGKGVPEDLVQHLFDPFVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRV
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHH
LLPMYRSSRSSEDN
HHHHHHCCCCCCCC
>Mature Secondary Structure
MKAELKPRTSDGEALTRAILDAVPHPVVTLAEDGRILDANVAAETFFQVSVPLLRRRPFA
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHCCCHH
EFVPFGTPLLSLIEQVRERGSSVNEYRVDLGTPRNGGERIVDIHVAPMAERPGHVVVMFQ
HHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEEC
ERTIAEKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIRGAAQLLEQSAGDEDRALTRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ICDEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLASSGFARHIRFIEDYDPSL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PPVYANRDQLIQVFLNLVKNAAEAIDSETDGEIHLTTAFRPGVRLTVPGASSKVSLPLEF
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCEECCHHE
CVRDNGKGVPEDLVQHLFDPFVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRV
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHH
LLPMYRSSRSSEDN
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]