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The map label for this gene is ntrC

Identifier: 158424710

GI number: 158424710

Start: 3531682

End: 3533124

Strand: Reverse

Name: ntrC

Synonym: AZC_3086

Alternate gene names: 158424710

Gene position: 3533124-3531682 (Counterclockwise)

Preceding gene: 158424711

Following gene: 158424709

Centisome position: 65.8

GC content: 67.78

Gene sequence:

>1443_bases
ATGCCTACCGGAAGCATCCTGGTCGCCGACGACGATGCCGCGATCCGCACCGTGCTCAATCAGGCCCTGTCGCGCGCCGG
CTATGAGGTGCGCTCCTGCGGGAACGCCGCCACGCTCTGGCGCTGGGTGAGCCAGGGCGACGGCGATCTCGTCATCACCG
ACGTGGTGATGCCCGACGAGAACGCCTTCGACCTGCTGCCGCGCATCAAGAAGGCGCGGCCGGACCTGCCGGTCATCGTC
ATGAGCGCGCAGAACACCTTCATGACCGCCATCCGCGCCTCCGAGCGCGGGGCCTATGAATATCTGCCCAAGCCCTTCGA
CCTGAAGGAGCTGATCTCCATCGTCGGCCGCGCGCTCGCCGAGCCGAAGAAGGCGGAGAAGCTCGCGCTCGGCTCCGACG
AGCAGGAGAATATCCCGCTGGTCGGCCGCTCGGCGGCCATGCAGGAGATCTACCGCCTCCTCGCCCGGCTGATGCAGACC
GACCTCACCGTGATGATCGCGGGCGAGAGCGGCACCGGCAAGGAACTGGTGGCCCGCGCGCTGCACGACTATGGCAAGCG
CCGCAACGGTCCCTTCGTCGCCATCAACATGGCGGCCATCCCGCGCGACCTCATCGAATCGGAGCTGTTCGGCCACGAGA
AGGGCTCGTTCACCGGCGCCACCACCCGCAACGCCGGCCGCTTCGAGCAGGCCGAGGGCGGCACGCTCTTCCTCGACGAG
ATCGGCGACATGCCCATGGAGGCGCAGACCCGCCTTCTGCGCGTGCTCCAGCAGGGCGAATATACGACGGTGGGCGGGCG
CACGCCCATCAAGACCGACGTGCGCATCATCGCCGCCACCAACAAGGACCTGCGGACGCTGATCCAGCAGGGCCTGTTCC
GCGAAGACCTGTTCTTCCGCCTGAACGTGGTGCCGCTGCGCCTGCCGCCGCTGCGCGAGCGCTCCGAGGATGTGCCGGAT
CTGGTGCGCCACTTCTTCCTTCAGGCCGAGCGCGAGGGCCTGCCCGCCAAGCAGGTGGACTCTGCCGCCCTCGACCGGCT
GAAGCGCTACCGCTGGCCGGGCAACGTGCGTGAGCTGGAAAACCTCGTGCGCCGCCTCGCCGCGCTTTATCCGCAGGAGA
TCATCACCCCCTCGATCATCGAGGCGGAACTGTCCGAGCCGGCGCCGAGCGCTTTGTCCGAGGACGGGCCCGTGGACGCG
ACGCTCGGAGCTTCCGTGGAGCGCCATCTCAACGGGTATTTCTCCGGCTTTGGCGAGGATCTGCCGCCTCCGGGCCTCTA
TCACCGCATCCTGCGGGACGTGGAATATCCGCTGCTCTCGGCCGCCCTCGCAGCCACGCGGGGCAACCAGATCAAGGCAG
CGGAGCTGCTCGGTCTGAACCGCAACACCCTGCGCAAGAAGATTCGGGACCTCGACATCCAGGTGATCCGCACCAGCCGC
TGA

Upstream 100 bases:

>100_bases
GGTATCGTCGAATGCGACAGCCAGCCCCGCCGCACCACGTTCCGCGTCCTGCTGCCCATGTACCGCAGCAGCCGGTCTTC
AGAGGACAACTGAGGTGAGC

Downstream 100 bases:

>100_bases
ACGGGACATCTCCTTGATGGCGTACGGGATGTCTGTCACCCTCCGGTTGTAAGGGGCCTGCATCGACGGCATCGCGGGTG
ATGCGGGCATTCAGGATGTG

Product: nitrogen assimilation regulatory protein ntrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPVIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKAEKLALGSDEQENIPLVGRSAAMQEIYRLLARLMQT
DLTVMIAGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNAGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDVPD
LVRHFFLQAEREGLPAKQVDSAALDRLKRYRWPGNVRELENLVRRLAALYPQEIITPSIIEAELSEPAPSALSEDGPVDA
TLGASVERHLNGYFSGFGEDLPPPGLYHRILRDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTSR

Sequences:

>Translated_480_residues
MPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPVIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKAEKLALGSDEQENIPLVGRSAAMQEIYRLLARLMQT
DLTVMIAGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNAGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDVPD
LVRHFFLQAEREGLPAKQVDSAALDRLKRYRWPGNVRELENLVRRLAALYPQEIITPSIIEAELSEPAPSALSEDGPVDA
TLGASVERHLNGYFSGFGEDLPPPGLYHRILRDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTSR
>Mature_479_residues
PTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDENAFDLLPRIKKARPDLPVIVM
SAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALAEPKKAEKLALGSDEQENIPLVGRSAAMQEIYRLLARLMQTD
LTVMIAGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNAGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFRLNVVPLRLPPLRERSEDVPDL
VRHFFLQAEREGLPAKQVDSAALDRLKRYRWPGNVRELENLVRRLAALYPQEIITPSIIEAELSEPAPSALSEDGPVDAT
LGASVERHLNGYFSGFGEDLPPPGLYHRILRDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTSR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1790299, Length=482, Percent_Identity=45.2282157676349, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=38.1052631578947, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=41.4163090128755, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1788905, Length=385, Percent_Identity=41.2987012987013, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1789087, Length=282, Percent_Identity=45.3900709219858, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=51.9480519480519, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=393, Percent_Identity=39.1857506361323, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=47.6190476190476, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081872, Length=339, Percent_Identity=38.3480825958702, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786524, Length=287, Percent_Identity=43.205574912892, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1787583, Length=245, Percent_Identity=40.8163265306122, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789828, Length=268, Percent_Identity=35.0746268656716, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081858, Length=209, Percent_Identity=34.9282296650718, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=28.7769784172662, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=33.6448598130841, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTRC_AZOC5 (Q04848)

Other databases:

- EMBL:   AP009384
- EMBL:   X63841
- PIR:   S18622
- RefSeq:   YP_001526002.1
- ProteinModelPortal:   Q04848
- SMR:   Q04848
- GeneID:   5692246
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3086
- HOGENOM:   HBG740884
- OMA:   GAQTRST
- ProtClustDB:   CLSK2298515
- BioCyc:   ACAU438753:AZC_3086-MONOMER
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00448
- TIGRFAMs:   TIGR01818

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 53254; Mature: 53123

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDE
CCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKARPDLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALA
CHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKKAEKLALGSDEQENIPLVGRSAAMQEIYRLLARLMQTDLTVMIAGESGTGKELVARA
CCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
LHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNAGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHEEE
LNVVPLRLPPLRERSEDVPDLVRHFFLQAEREGLPAKQVDSAALDRLKRYRWPGNVRELE
EEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLVRRLAALYPQEIITPSIIEAELSEPAPSALSEDGPVDATLGASVERHLNGYFSGFGED
HHHHHHHHHCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LPPPGLYHRILRDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTSR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHCCEEEEECCC
>Mature Secondary Structure 
PTGSILVADDDAAIRTVLNQALSRAGYEVRSCGNAATLWRWVSQGDGDLVITDVVMPDE
CCCCEEEECCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKARPDLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELISIVGRALA
CHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKKAEKLALGSDEQENIPLVGRSAAMQEIYRLLARLMQTDLTVMIAGESGTGKELVARA
CCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
LHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGSFTGATTRNAGRFEQAEGGTLFLDE
HHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIIAATNKDLRTLIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHEEE
LNVVPLRLPPLRERSEDVPDLVRHFFLQAEREGLPAKQVDSAALDRLKRYRWPGNVRELE
EEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLVRRLAALYPQEIITPSIIEAELSEPAPSALSEDGPVDATLGASVERHLNGYFSGFGED
HHHHHHHHHCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LPPPGLYHRILRDVEYPLLSAALAATRGNQIKAAELLGLNRNTLRKKIRDLDIQVIRTSR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370