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The map label for this gene is ntrX

Identifier: 158424707

GI number: 158424707

Start: 3525461

End: 3526825

Strand: Reverse

Name: ntrX

Synonym: AZC_3083

Alternate gene names: 158424707

Gene position: 3526825-3525461 (Counterclockwise)

Preceding gene: 158424708

Following gene: 158424706

Centisome position: 65.68

GC content: 68.21

Gene sequence:

>1365_bases
ATGGCCCATGACATCCTGATCGTCGATGACGAGCCCGACATCAGCGGCCTCGTCGCCGGCATCCTGGAGGACGAGGGCTA
TTCCGCCCGCACCGCCCGCGACGCCGATGGCGCGCTGGCCGAGATCGCCGCGCGGCGGCCGAACCTGATCTTCCTCGACA
TCTGGCTGCAGGGCAGCCGCCTCGACGGCCTCGAATTGCTGGACATCATCAAGCGCGAGCACCCCGAGGTGCCGGTGGTG
ATGATCTCCGGCCACGGCAACATCGAGACGGCGGTGGCCGCCATCAAGCGCGGCGCCTACGACTTCATCGAGAAGCCCTT
CAATGCCGACCGGCTGGTGGTCATCACCGAGCGGGCGCTGGAGACGCTGCGGCTGCGCCGCGAGGTACGGGAGCTGAAGC
AGCTCACCCAGCCGCACACCATGGTGGGCCGCTCCAGCGTCATCCAGCAGTTGCGCGCCACCGTGGACCGAGTCGGCCCC
ACCAACAGCCGCATCCTCATCGTCGGCCCCTCGGGCTCCGGCAAGGAGCTGACCGCGCGCATGATCCATGCGGCCTCCGC
CCGCGCGCAGGGGCCGTTCGTGGTCATCAACGCCGCCGCCATCACCCCCGAGCGCCTCGAATATGAGCTGTTCGGCGTGG
AGGAGGGGGAAGGGCGCGAGCGCCATCGCGGCGCGCTGGAAGAGGCGCACGGCGGCACTCTGTTCCTCGACGAGATCGCC
GACATGCCGCGCGAGACCCAGAACCGGGTGCTGCGGGTGCTGGTGGAGCAGACCTTCAGCCGCATCGGCAGCAGCGAGAA
GGTGCGCGTGGACGTGCGCATCATCTCCTCCACCGGGCGTCACCTGGAGGAAGAGATCGCCGCCGGGCGTTTCCGCGAGG
ATCTCTACCACCGCCTTTCGGTGGTGCCGATCCGCGTGCCGCCGCTGGCCGAGCGGCGGGAGGACATCCCCGATCTCGTG
GATTTCTTCATCGACCTCATCTCTCAGACGACGGGCCTTCAGCGCCGCAAGGTGGGCGAGGATGCCATGGCCGTGCTCCA
GTCCCATGACTGGCCGGGCAACGTGCGGCAGCTGCGCAACAATGTGGAGCGCCTGCTGATTCTCGCCGGCGGCGATCCGG
ATGCGGAGGTGACCGCCTCCATGCTGCCGCCGGACGTGGGTGCGCTGGTGCCCACCCTGCCCAACGGCAATGGCGGCGAG
CATCTGATGGGCCTGCCGCTGCGTGAGGCCCGCGAAGTGTTCGAGCGCGAATATCTCGCAGCGCAGATTAACCGCTTTGG
CGGCAATATCTCGCGTACGGCGGAATTCGTCGGCATGGAACGCTCGGCCCTGCATCGCAAGCTGAAGGCGCTCGGCGTAG
GCTGA

Upstream 100 bases:

>100_bases
GACCGGGGCTGCCACGCCCGCGGCGCCCGCCGCTTCCGCCATGGCCCGCGATGCGGCTGCCGATTCCGCCGCGCGCGGCA
AGAACGAGAGGACCTGATCC

Downstream 100 bases:

>100_bases
CGGAGCGGGACGGGATAAGGTTCGCGGCGGCGCGGTGTTGCAGGGGCGGCAGCCGCGCCGGGAAAACGGGATCGGCATTC
GGGCCAAGGGCTTGCGGGTG

Product: nitrogen assimilation regulatory protein ntrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAHDILIVDDEPDISGLVAGILEDEGYSARTARDADGALAEIAARRPNLIFLDIWLQGSRLDGLELLDIIKREHPEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFNADRLVVITERALETLRLRREVRELKQLTQPHTMVGRSSVIQQLRATVDRVGP
TNSRILIVGPSGSGKELTARMIHAASARAQGPFVVINAAAITPERLEYELFGVEEGEGRERHRGALEEAHGGTLFLDEIA
DMPRETQNRVLRVLVEQTFSRIGSSEKVRVDVRIISSTGRHLEEEIAAGRFREDLYHRLSVVPIRVPPLAERREDIPDLV
DFFIDLISQTTGLQRRKVGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDPDAEVTASMLPPDVGALVPTLPNGNGGE
HLMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG

Sequences:

>Translated_454_residues
MAHDILIVDDEPDISGLVAGILEDEGYSARTARDADGALAEIAARRPNLIFLDIWLQGSRLDGLELLDIIKREHPEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFNADRLVVITERALETLRLRREVRELKQLTQPHTMVGRSSVIQQLRATVDRVGP
TNSRILIVGPSGSGKELTARMIHAASARAQGPFVVINAAAITPERLEYELFGVEEGEGRERHRGALEEAHGGTLFLDEIA
DMPRETQNRVLRVLVEQTFSRIGSSEKVRVDVRIISSTGRHLEEEIAAGRFREDLYHRLSVVPIRVPPLAERREDIPDLV
DFFIDLISQTTGLQRRKVGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDPDAEVTASMLPPDVGALVPTLPNGNGGE
HLMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG
>Mature_453_residues
AHDILIVDDEPDISGLVAGILEDEGYSARTARDADGALAEIAARRPNLIFLDIWLQGSRLDGLELLDIIKREHPEVPVVM
ISGHGNIETAVAAIKRGAYDFIEKPFNADRLVVITERALETLRLRREVRELKQLTQPHTMVGRSSVIQQLRATVDRVGPT
NSRILIVGPSGSGKELTARMIHAASARAQGPFVVINAAAITPERLEYELFGVEEGEGRERHRGALEEAHGGTLFLDEIAD
MPRETQNRVLRVLVEQTFSRIGSSEKVRVDVRIISSTGRHLEEEIAAGRFREDLYHRLSVVPIRVPPLAERREDIPDLVD
FFIDLISQTTGLQRRKVGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDPDAEVTASMLPPDVGALVPTLPNGNGGEH
LMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=36.1702127659575, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=37.280701754386, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=463, Percent_Identity=34.7732181425486, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1788905, Length=455, Percent_Identity=36.043956043956, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=37.4251497005988, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=36.890243902439, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI87082152, Length=327, Percent_Identity=37.6146788990826, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=42.5531914893617, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=37.2670807453416, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=33.1288343558282, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=30.4498269896194, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789828, Length=216, Percent_Identity=35.6481481481481, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786911, Length=186, Percent_Identity=31.7204301075269, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTRX_AZOC5 (Q04849)

Other databases:

- EMBL:   X63841
- EMBL:   AP009384
- PIR:   S18625
- RefSeq:   YP_001525999.1
- ProteinModelPortal:   Q04849
- SMR:   Q04849
- GeneID:   5692245
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3083
- HOGENOM:   HBG740884
- OMA:   KRQIGQD
- ProtClustDB:   CLSK862979
- BioCyc:   ACAU438753:AZC_3083-MONOMER
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 50221; Mature: 50090

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDILIVDDEPDISGLVAGILEDEGYSARTARDADGALAEIAARRPNLIFLDIWLQGSR
CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCC
LDGLELLDIIKREHPEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFNADRLVVITERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHH
ETLRLRREVRELKQLTQPHTMVGRSSVIQQLRATVDRVGPTNSRILIVGPSGSGKELTAR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
MIHAASARAQGPFVVINAAAITPERLEYELFGVEEGEGRERHRGALEEAHGGTLFLDEIA
HHHHHHCCCCCCEEEEEECCCCHHHCEEEEECCCCCCCHHHHHCHHHHCCCCEEEHHHHH
DMPRETQNRVLRVLVEQTFSRIGSSEKVRVDVRIISSTGRHLEEEIAAGRFREDLYHRLS
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHE
VVPIRVPPLAERREDIPDLVDFFIDLISQTTGLQRRKVGEDAMAVLQSHDWPGNVRQLRN
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHHHHHHH
NVERLLILAGGDPDAEVTASMLPPDVGALVPTLPNGNGGEHLMGLPLREAREVFEREYLA
HHHEEEEEECCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AHDILIVDDEPDISGLVAGILEDEGYSARTARDADGALAEIAARRPNLIFLDIWLQGSR
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEECCC
LDGLELLDIIKREHPEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFNADRLVVITERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHH
ETLRLRREVRELKQLTQPHTMVGRSSVIQQLRATVDRVGPTNSRILIVGPSGSGKELTAR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
MIHAASARAQGPFVVINAAAITPERLEYELFGVEEGEGRERHRGALEEAHGGTLFLDEIA
HHHHHHCCCCCCEEEEEECCCCHHHCEEEEECCCCCCCHHHHHCHHHHCCCCEEEHHHHH
DMPRETQNRVLRVLVEQTFSRIGSSEKVRVDVRIISSTGRHLEEEIAAGRFREDLYHRLS
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHE
VVPIRVPPLAERREDIPDLVDFFIDLISQTTGLQRRKVGEDAMAVLQSHDWPGNVRQLRN
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHHHHHHH
NVERLLILAGGDPDAEVTASMLPPDVGALVPTLPNGNGGEHLMGLPLREAREVFEREYLA
HHHEEEEEECCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370