Click here to switch to the map view.

The map label for this gene is eno

Identifier: 158424685

GI number: 158424685

Start: 3499701

End: 3500984

Strand: Reverse

Name: eno

Synonym: AZC_3061

Alternate gene names: 158424685

Gene position: 3500984-3499701 (Counterclockwise)

Preceding gene: 158424688

Following gene: 158424683

Centisome position: 65.2

GC content: 66.59

Gene sequence:

>1284_bases
ATGACCGCCATTGTCGATATCGTCGGCCGGGAAATCCTGGACAGCCGCGGCAACCCCACGGTGGAAGTCGATGTGCTGCT
GGAAGACGGCGCGCTCGGTCGCGCGGCGGTTCCCTCCGGCGCGTCCACGGGCGCCCATGAGGCGGTCGAACTGCGCGACG
GCGACACGGGCCGCTATCTCGGCAAGGGCGTCGAGAAGGCGGTCGAGGCGGTGAACGGCGAGATCTTCGACGCCATCGGC
GGCCTCGATGCCGAGGAGCAGGCGCAGATCGACGCCGTCATGATCGAACTCGACGGCACGCCCAACAAGGCCCGCCTCGG
CGCCAACGCCATCCTCGGCGTGTCGCTGGCGGTGGCCAAGGCGGCCGCCATCTCCAACAACCTGCCGCTCTATCGTTATG
TGGGCGGCGTGAACGCCCGCACCCTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGCGCATGCGGACAATCCCATC
GACTTCCAGGAATTCATGATCCTGCCCGCCGGCGCGCCGACCTTCGCGGAAGGCCTGCGCTGGGGCGCCGAGATCTTCCA
CACCCTGAAGAAGGGCCTGAAGGACGCCGGCCATAACACCAACGTGGGCGACGAGGGCGGCTTCGCCCCGAACCTTGCGT
CTGCCGAGGCGGCGCTCGACTTCGTGCTGAAGGCCATCGAGAAGGCCGGCTTCAAGCCGGGCGAGGACGTGTTCCTCGGC
CTCGACTGCGCCGCCACCGAATTCTTCAAGGACGGCGCCTATCACTATGAGGGCGAGGGCAAGGTCCGCGACATCGACGC
CCAGGTGAAGTATCTGGCGCAGCTCGTCGGCAACTACCCGATCGTCACCATCGAGGACGGCATGTCCGAGGACGACTGGG
CGGGCTGGAAGCAGCTCACCGACGCCATCGGCGCCAAGTGCCAGCTTGTGGGCGACGACCTGTTCGTGACCAATGTCGAG
CGCCTCGGCCGTGGCATCAAGGAAGCCACCGGCAACGCCATCCTCGTGAAGGTGAACCAGATCGGCTCGCTGACCGAGAC
GCTGGAGACGGTGGAACTCGCCCACAAGTCCGGCTATCGCGCCGTGATGTCCCATCGTTCGGGCGAGACCGAGGACTCCA
CCATCGCCGATCTCGCGGTGGCCACGAACTGCGGTCAGATCAAGACCGGCTCGCTCGCCCGCTCCGACCGCACCGCCAAG
TACAACCAGCTCCTGCGCATCGAGCAGGAACTGGGCGCACAGGCGAAATATGCCGGCAAGGCGGCGCTCAAGGCCTTCGC
CTGA

Upstream 100 bases:

>100_bases
CTTACCAGCGGCGGCGTGACAGGCTAGAAGCGCAGCGCTCCGTTTCGCATCCGCGCGCCGCGCGGGTCACGCTTTGGGCA
GAGGAAGGGAAGGACGCTCC

Downstream 100 bases:

>100_bases
GCCTTCAGGTCGATCGTTGAATGACGAGGCCTCCGCGCTCCGGCGCGGGGGCCTTTTTCTTTGGCGGATGGGACAGGGTT
CAGAGTGCGTGGCGCAGGCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAIVDIVGREILDSRGNPTVEVDVLLEDGALGRAAVPSGASTGAHEAVELRDGDTGRYLGKGVEKAVEAVNGEIFDAIG
GLDAEEQAQIDAVMIELDGTPNKARLGANAILGVSLAVAKAAAISNNLPLYRYVGGVNARTLPVPMMNIINGGAHADNPI
DFQEFMILPAGAPTFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALDFVLKAIEKAGFKPGEDVFLG
LDCAATEFFKDGAYHYEGEGKVRDIDAQVKYLAQLVGNYPIVTIEDGMSEDDWAGWKQLTDAIGAKCQLVGDDLFVTNVE
RLGRGIKEATGNAILVKVNQIGSLTETLETVELAHKSGYRAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELGAQAKYAGKAALKAFA

Sequences:

>Translated_427_residues
MTAIVDIVGREILDSRGNPTVEVDVLLEDGALGRAAVPSGASTGAHEAVELRDGDTGRYLGKGVEKAVEAVNGEIFDAIG
GLDAEEQAQIDAVMIELDGTPNKARLGANAILGVSLAVAKAAAISNNLPLYRYVGGVNARTLPVPMMNIINGGAHADNPI
DFQEFMILPAGAPTFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALDFVLKAIEKAGFKPGEDVFLG
LDCAATEFFKDGAYHYEGEGKVRDIDAQVKYLAQLVGNYPIVTIEDGMSEDDWAGWKQLTDAIGAKCQLVGDDLFVTNVE
RLGRGIKEATGNAILVKVNQIGSLTETLETVELAHKSGYRAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELGAQAKYAGKAALKAFA
>Mature_426_residues
TAIVDIVGREILDSRGNPTVEVDVLLEDGALGRAAVPSGASTGAHEAVELRDGDTGRYLGKGVEKAVEAVNGEIFDAIGG
LDAEEQAQIDAVMIELDGTPNKARLGANAILGVSLAVAKAAAISNNLPLYRYVGGVNARTLPVPMMNIINGGAHADNPID
FQEFMILPAGAPTFAEGLRWGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALDFVLKAIEKAGFKPGEDVFLGL
DCAATEFFKDGAYHYEGEGKVRDIDAQVKYLAQLVGNYPIVTIEDGMSEDDWAGWKQLTDAIGAKCQLVGDDLFVTNVER
LGRGIKEATGNAILVKVNQIGSLTETLETVELAHKSGYRAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLLRIEQELGAQAKYAGKAALKAFA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=53.6130536130536, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=50.9216589861751, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=50.8083140877598, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=50.8083140877598, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=46.6357308584687, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=58.5648148148148, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.3148148148148, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.3148148148148, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=49.2063492063492, Blast_Score=177, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=48.6111111111111, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=47.7064220183486, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=48.3796296296296, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=48.3796296296296, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=47.4770642201835, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=49.7695852534562, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=49.7695852534562, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=49.7695852534562, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=49.7695852534562, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=49.7695852534562, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=49.7695852534562, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_AZOC5 (A8IBF3)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525977.1
- ProteinModelPortal:   A8IBF3
- SMR:   A8IBF3
- GeneID:   5691701
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_3061
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   ACAU438753:AZC_3061-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45072; Mature: 44941

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIVGREILDSRGNPTVEVDVLLEDGALGRAAVPSGASTGAHEAVELRDGDTGRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVEKAVEAVNGEIFDAIGGLDAEEQAQIDAVMIELDGTPNKARLGANAILGVSLAVAK
HHHHHHHHHHHCCHHHHHHCCCCCHHHCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAAISNNLPLYRYVGGVNARTLPVPMMNIINGGAHADNPIDFQEFMILPAGAPTFAEGLR
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
WGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALDFVLKAIEKAGFKPGEDVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAATEFFKDGAYHYEGEGKVRDIDAQVKYLAQLVGNYPIVTIEDGMSEDDWAGWKQLT
CHHHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
DAIGAKCQLVGDDLFVTNVERLGRGIKEATGNAILVKVNQIGSLTETLETVELAHKSGYR
HHHCCEEEEECCCHHEECHHHHCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELGAQAKYAGK
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AALKAFA
HHHHHCC
>Mature Secondary Structure 
TAIVDIVGREILDSRGNPTVEVDVLLEDGALGRAAVPSGASTGAHEAVELRDGDTGRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVEKAVEAVNGEIFDAIGGLDAEEQAQIDAVMIELDGTPNKARLGANAILGVSLAVAK
HHHHHHHHHHHCCHHHHHHCCCCCHHHCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAAISNNLPLYRYVGGVNARTLPVPMMNIINGGAHADNPIDFQEFMILPAGAPTFAEGLR
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
WGAEIFHTLKKGLKDAGHNTNVGDEGGFAPNLASAEAALDFVLKAIEKAGFKPGEDVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAATEFFKDGAYHYEGEGKVRDIDAQVKYLAQLVGNYPIVTIEDGMSEDDWAGWKQLT
CHHHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
DAIGAKCQLVGDDLFVTNVERLGRGIKEATGNAILVKVNQIGSLTETLETVELAHKSGYR
HHHCCEEEEECCCHHEECHHHHCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELGAQAKYAGK
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AALKAFA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA