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The map label for this gene is ooxB [H]

Identifier: 158424672

GI number: 158424672

Start: 3483402

End: 3484520

Strand: Direct

Name: ooxB [H]

Synonym: AZC_3048

Alternate gene names: 158424672

Gene position: 3483402-3484520 (Clockwise)

Preceding gene: 158424671

Following gene: 158424674

Centisome position: 64.87

GC content: 69.17

Gene sequence:

>1119_bases
ATGAGCACCCAAGAGACCGAGGTTCTCATCATCGGCGCGGGCGGGGCGGGAACGTCGGCGGCATTGCATCTGGCGCAGCG
GGGCGTGGCCTGCGTGCTGCTGGAGCGGGGACTGGTGGGCGGGCAGGCCTCGGGTGTCAATTATGGCGGGGTGCGCCAGC
AGGGGCGGCATCCCGCGGAACTGCCCATCGCCCGGCGCTCCATGGCGCTCTGGCACAAGCTCAAGGACATCACCGGAACC
GACGCCGAATTCACCGTCAGCGGCCATCTGAAGCTCGCCCGGACGCCGGAAGAAGAAGCCGACCTCGTCGCCTATCTGGA
GGTCGCCCGCGACAACGACCTGCCGCTGCGCTTCGTCGGCCGCAACGCCCTGCATGAGGATTATCCCTGGCTGGGTCCGA
AGGTGATCGCCGGTTCACTGGCGCCCGAGGACGGCGCCGCCAACCCCCGCCTCATGGTCCCCGCCCTCGCCCATGCGGCA
CGGGCGGCGGGCGCCGTCATCCACGAGAACACCAAGGCCACGCGCTATGCCTTGGACGGCGAGTGTTTCGAGGTCACGAC
CGGCACGCACACCTTCCGCGCCCGCTGGCTGCTGAACACCGCCGGATTCTGGGGCGGCGCGGTGGCCGAAGCGTTCGGGG
AGAAGACGCCGGTCTCCGCCCTCAATCCCAACATGATGGTGACGGAGCCCATCCGCCATTTCATCGAACCGAACCTCGGC
GTGGTGGGCGGCAATGTCTATCTGCGGCAGATCCCGCGCGGCAATGTCATCATCGGCGGCGGCTATGGGGTGAGCGACCC
CGCCGTGCCATGGTCGCGCCCGAAGGCCGAGGAGACGCGCGCGGCCATGGCGCTGGCGGTGGACCTGGTGCCGGAACTGA
AGGGCATCTCGATCATCCGCTCCTGGACCGGCATCGATGGCGAGATGCCCGACCATATTCCGGTGATCGGCCCGAGCCGC
ACCACGCCCCGCCTCCTCCATGCCTTCGGCTTTTCCGGGCACGGCTTTCAGCTCGGTCCTGCGGTGGGCGCGATCCTCAG
CGAACTGGTGCTCGACGGGCGCACCGACGTGCCGCTTGAGCCCTTCGACATCGGCCGCTGGACGGCGCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
TGCCCTCCCGCGCAAGGGGGGAGGGTTCGGCCGGGCAACCGCCAGGGGCGGCCAGCAGGCGCGCGAGGTTGTTGCGCCAT
GATCCGTCCGGGAGCCTGCC

Downstream 100 bases:

>100_bases
CGCGAGGTCGGAAACAGGCCCGGCGCGGCTCAGTCGTAGATGCGTGGCAGCGGCTCGCGCGCGCCCTTGCGGCCCGCCGG
CACGGCGGCCTTGCGGAAGG

Product: FAD dependent oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSTQETEVLIIGAGGAGTSAALHLAQRGVACVLLERGLVGGQASGVNYGGVRQQGRHPAELPIARRSMALWHKLKDITGT
DAEFTVSGHLKLARTPEEEADLVAYLEVARDNDLPLRFVGRNALHEDYPWLGPKVIAGSLAPEDGAANPRLMVPALAHAA
RAAGAVIHENTKATRYALDGECFEVTTGTHTFRARWLLNTAGFWGGAVAEAFGEKTPVSALNPNMMVTEPIRHFIEPNLG
VVGGNVYLRQIPRGNVIIGGGYGVSDPAVPWSRPKAEETRAAMALAVDLVPELKGISIIRSWTGIDGEMPDHIPVIGPSR
TTPRLLHAFGFSGHGFQLGPAVGAILSELVLDGRTDVPLEPFDIGRWTARAA

Sequences:

>Translated_372_residues
MSTQETEVLIIGAGGAGTSAALHLAQRGVACVLLERGLVGGQASGVNYGGVRQQGRHPAELPIARRSMALWHKLKDITGT
DAEFTVSGHLKLARTPEEEADLVAYLEVARDNDLPLRFVGRNALHEDYPWLGPKVIAGSLAPEDGAANPRLMVPALAHAA
RAAGAVIHENTKATRYALDGECFEVTTGTHTFRARWLLNTAGFWGGAVAEAFGEKTPVSALNPNMMVTEPIRHFIEPNLG
VVGGNVYLRQIPRGNVIIGGGYGVSDPAVPWSRPKAEETRAAMALAVDLVPELKGISIIRSWTGIDGEMPDHIPVIGPSR
TTPRLLHAFGFSGHGFQLGPAVGAILSELVLDGRTDVPLEPFDIGRWTARAA
>Mature_371_residues
STQETEVLIIGAGGAGTSAALHLAQRGVACVLLERGLVGGQASGVNYGGVRQQGRHPAELPIARRSMALWHKLKDITGTD
AEFTVSGHLKLARTPEEEADLVAYLEVARDNDLPLRFVGRNALHEDYPWLGPKVIAGSLAPEDGAANPRLMVPALAHAAR
AAGAVIHENTKATRYALDGECFEVTTGTHTFRARWLLNTAGFWGGAVAEAFGEKTPVSALNPNMMVTEPIRHFIEPNLGV
VGGNVYLRQIPRGNVIIGGGYGVSDPAVPWSRPKAEETRAAMALAVDLVPELKGISIIRSWTGIDGEMPDHIPVIGPSRT
TPRLLHAFGFSGHGFQLGPAVGAILSELVLDGRTDVPLEPFDIGRWTARAA

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=397, Percent_Identity=27.9596977329975, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI197927446, Length=385, Percent_Identity=25.1948051948052, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI21361378, Length=385, Percent_Identity=25.1948051948052, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI8923708, Length=391, Percent_Identity=23.7851662404092, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71994045, Length=403, Percent_Identity=25.0620347394541, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=410, Percent_Identity=24.8780487804878, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI20130091, Length=381, Percent_Identity=24.6719160104987, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 39575; Mature: 39443

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQETEVLIIGAGGAGTSAALHLAQRGVACVLLERGLVGGQASGVNYGGVRQQGRHPAE
CCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCC
LPIARRSMALWHKLKDITGTDAEFTVSGHLKLARTPEEEADLVAYLEVARDNDLPLRFVG
CCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHHEEEEEHHCCCCCCEEEEC
RNALHEDYPWLGPKVIAGSLAPEDGAANPRLMVPALAHAARAAGAVIHENTKATRYALDG
CCCCCCCCCCCCCHHEECCCCCCCCCCCCEEEHHHHHHHHHHHCCEEECCCCCEEEEECC
ECFEVTTGTHTFRARWLLNTAGFWGGAVAEAFGEKTPVSALNPNMMVTEPIRHFIEPNLG
CEEEEECCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCE
VVGGNVYLRQIPRGNVIIGGGYGVSDPAVPWSRPKAEETRAAMALAVDLVPELKGISIIR
EEECCEEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SWTGIDGEMPDHIPVIGPSRTTPRLLHAFGFSGHGFQLGPAVGAILSELVLDGRTDVPLE
ECCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCC
PFDIGRWTARAA
CCCCCCEEECCC
>Mature Secondary Structure 
STQETEVLIIGAGGAGTSAALHLAQRGVACVLLERGLVGGQASGVNYGGVRQQGRHPAE
CCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCC
LPIARRSMALWHKLKDITGTDAEFTVSGHLKLARTPEEEADLVAYLEVARDNDLPLRFVG
CCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHHEEEEEHHCCCCCCEEEEC
RNALHEDYPWLGPKVIAGSLAPEDGAANPRLMVPALAHAARAAGAVIHENTKATRYALDG
CCCCCCCCCCCCCHHEECCCCCCCCCCCCEEEHHHHHHHHHHHCCEEECCCCCEEEEECC
ECFEVTTGTHTFRARWLLNTAGFWGGAVAEAFGEKTPVSALNPNMMVTEPIRHFIEPNLG
CEEEEECCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCCCE
VVGGNVYLRQIPRGNVIIGGGYGVSDPAVPWSRPKAEETRAAMALAVDLVPELKGISIIR
EEECCEEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SWTGIDGEMPDHIPVIGPSRTTPRLLHAFGFSGHGFQLGPAVGAILSELVLDGRTDVPLE
ECCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCC
PFDIGRWTARAA
CCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]