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The map label for this gene is gsiB [H]

Identifier: 158424665

GI number: 158424665

Start: 3474516

End: 3476078

Strand: Direct

Name: gsiB [H]

Synonym: AZC_3041

Alternate gene names: 158424665

Gene position: 3474516-3476078 (Clockwise)

Preceding gene: 158424664

Following gene: 158424666

Centisome position: 64.71

GC content: 65.9

Gene sequence:

>1563_bases
TTGCCGCCCCCGCCCGCTTTCGCGGAGACGATGGACATGGCCGACCTGACCCGACGCTCAGTTCTCACCGGCGCCGCAGC
GGCGGCCATGGTGCCGCTGCTGCGCATTCCCGGCGCCAAGGCGCGCACGCCCGGCATTCTGACCTTCGGCATCTCCTCCT
TCCCGCCCAGCGTCCAGCCCTGGGCCAATGCCGGCACCGCTTCCGCCACGGTGAAGCTCGCACTCTATCGCGGCCTCACC
TCCTATGGGCCGGACGGCAAGCTGCGTGGCGAGCTGGCGGAAAGCTGGGCGCCGGAAGGCAAGAATGTCTGGGTGTTCAA
GCTGCGCGACGCCACCTTCTCCAACGGCGACAAGGTCACGTCGGCGGATGTGAAGTGGACCATCGAGCAGGTGGCCGGCG
AGAAATCCACCGCCTTCCTGCGCAACGAGATGCAGGGCGTGGAGAAGATCGAGACGCCCGACGACAAGACCGTCCGCCTC
ACCATGAAGCAGCCCTCGGCGACGCTGCCGCTGCTGATGGCGAGCTACCACATGCCCATCATGTCGAAGGCTTCCACCGC
CTCGCAGCAGATCGGCGCCGGCCCGTTCATCCTCAAGGATGCCGAGCGCGGCGTCTCCATGGATCTGGAAGCCAATCCCA
AATACTACCGGCCCGGCCTGCCCAAGCTGAAAGGCATCCGCGTCATGGTCTATGCGGATGAGAACCTGCGCGTCGCAGCG
CTCCAGAGCGGCGACGTGGACCTCATCGAATATGTGCCGTGGCAGGCCATGACCAGCATCGAGCAGAATGCGCAGCTGAA
GCTCGATGCCGTGGACGGCCCGTTCATGTCGCTGATCTTCAATGGCTCGCGCCCGCCCTTCAACGATCCGAAGGTGCGCA
AGGCGGTGGCCCACGCCATCCGCCGCGAGGAGATCGTGAAGAGCGCCTTCTTCGGCCGCGGCGCGCCGCTGGCGCATCTG
CCCATCCCGAAGGCGAGCCCCTTCTTCAACGCCGATCTGGCGGAAGGCTGGGCCTATAATCCGGACCTCTCCAAGAAGCT
GCTGGCGGAGGCCGGGCATGGCGGCGGCCTCTCCTGCAAGCTGCTCTCCACGGCGCAGTACGGCATGCACAAGGACACGG
CGGAAGTGGTGCAGCAGCATCTCGCCGCCGTGGGCATCAATGTGGAGCTGAACCTGCCGGACTGGGCGACCCGCGTGGCC
CTGGGCAATCGCGGCCAATATGATCTGGCGGTGATGGGCACGTCTGCGGACAGCAACGACCCGGACGGCCTTTCGGCCTA
TGTGGACGGCAGCCTCTCGCCATCTTACGTGCGCAGCTTCAACCTCTCCATTCCGAAGCTCACCGAGCTGTTCCAGGCCG
GCCGCGAGGAATTCGACGAGGCCAAGCGCAAGGCCATCTATCACGAGCTGGAACAGGTGGCGATCGACCAGGTGCCCATG
GTGGGCCTCGCCCTGCGCAGCCAGGGCTACGCCATGCGCAAGGACGTGACCGGCTTCACCAACATGCCCGGCGCGCTCAC
CTTCTATTCCGGCGAGACCTTCGAAACGACCTCGGTGGGCTGA

Upstream 100 bases:

>100_bases
CCTCTTTGCCGGCCGCCGCTTCCTTCAAGGCCGCGCGCCCGAGAGGCGCGCCGCATCTTCACGCCGACGCCTTTTCTGTC
CCATTCCGGGCCGGGCGCCG

Downstream 100 bases:

>100_bases
TGAACGCGCGCGCGGACATCGGGATGGATGGGCGGGCCAGTCCCCTCCCCCTTTTCGGGGGCGGGTTCGGGAGGGGAGCC
GGACGCTCTCTCCATGCCCG

Product: twin-arginine translocation pathway signal precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MPPPPAFAETMDMADLTRRSVLTGAAAAAMVPLLRIPGAKARTPGILTFGISSFPPSVQPWANAGTASATVKLALYRGLT
SYGPDGKLRGELAESWAPEGKNVWVFKLRDATFSNGDKVTSADVKWTIEQVAGEKSTAFLRNEMQGVEKIETPDDKTVRL
TMKQPSATLPLLMASYHMPIMSKASTASQQIGAGPFILKDAERGVSMDLEANPKYYRPGLPKLKGIRVMVYADENLRVAA
LQSGDVDLIEYVPWQAMTSIEQNAQLKLDAVDGPFMSLIFNGSRPPFNDPKVRKAVAHAIRREEIVKSAFFGRGAPLAHL
PIPKASPFFNADLAEGWAYNPDLSKKLLAEAGHGGGLSCKLLSTAQYGMHKDTAEVVQQHLAAVGINVELNLPDWATRVA
LGNRGQYDLAVMGTSADSNDPDGLSAYVDGSLSPSYVRSFNLSIPKLTELFQAGREEFDEAKRKAIYHELEQVAIDQVPM
VGLALRSQGYAMRKDVTGFTNMPGALTFYSGETFETTSVG

Sequences:

>Translated_520_residues
MPPPPAFAETMDMADLTRRSVLTGAAAAAMVPLLRIPGAKARTPGILTFGISSFPPSVQPWANAGTASATVKLALYRGLT
SYGPDGKLRGELAESWAPEGKNVWVFKLRDATFSNGDKVTSADVKWTIEQVAGEKSTAFLRNEMQGVEKIETPDDKTVRL
TMKQPSATLPLLMASYHMPIMSKASTASQQIGAGPFILKDAERGVSMDLEANPKYYRPGLPKLKGIRVMVYADENLRVAA
LQSGDVDLIEYVPWQAMTSIEQNAQLKLDAVDGPFMSLIFNGSRPPFNDPKVRKAVAHAIRREEIVKSAFFGRGAPLAHL
PIPKASPFFNADLAEGWAYNPDLSKKLLAEAGHGGGLSCKLLSTAQYGMHKDTAEVVQQHLAAVGINVELNLPDWATRVA
LGNRGQYDLAVMGTSADSNDPDGLSAYVDGSLSPSYVRSFNLSIPKLTELFQAGREEFDEAKRKAIYHELEQVAIDQVPM
VGLALRSQGYAMRKDVTGFTNMPGALTFYSGETFETTSVG
>Mature_519_residues
PPPPAFAETMDMADLTRRSVLTGAAAAAMVPLLRIPGAKARTPGILTFGISSFPPSVQPWANAGTASATVKLALYRGLTS
YGPDGKLRGELAESWAPEGKNVWVFKLRDATFSNGDKVTSADVKWTIEQVAGEKSTAFLRNEMQGVEKIETPDDKTVRLT
MKQPSATLPLLMASYHMPIMSKASTASQQIGAGPFILKDAERGVSMDLEANPKYYRPGLPKLKGIRVMVYADENLRVAAL
QSGDVDLIEYVPWQAMTSIEQNAQLKLDAVDGPFMSLIFNGSRPPFNDPKVRKAVAHAIRREEIVKSAFFGRGAPLAHLP
IPKASPFFNADLAEGWAYNPDLSKKLLAEAGHGGGLSCKLLSTAQYGMHKDTAEVVQQHLAAVGINVELNLPDWATRVAL
GNRGQYDLAVMGTSADSNDPDGLSAYVDGSLSPSYVRSFNLSIPKLTELFQAGREEFDEAKRKAIYHELEQVAIDQVPMV
GLALRSQGYAMRKDVTGFTNMPGALTFYSGETFETTSVG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=476, Percent_Identity=26.890756302521, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787762, Length=518, Percent_Identity=26.4478764478764, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789966, Length=375, Percent_Identity=24, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=511, Percent_Identity=24.0704500978474, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787551, Length=521, Percent_Identity=25.3358925143954, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081878, Length=490, Percent_Identity=22.0408163265306, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=22.1995926680244, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56275; Mature: 56144

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPPAFAETMDMADLTRRSVLTGAAAAAMVPLLRIPGAKARTPGILTFGISSFPPSVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCC
WANAGTASATVKLALYRGLTSYGPDGKLRGELAESWAPEGKNVWVFKLRDATFSNGDKVT
CCCCCCCHHEEEEEEHHCCHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEE
SADVKWTIEQVAGEKSTAFLRNEMQGVEKIETPDDKTVRLTMKQPSATLPLLMASYHMPI
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCH
MSKASTASQQIGAGPFILKDAERGVSMDLEANPKYYRPGLPKLKGIRVMVYADENLRVAA
HHCCCHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEE
LQSGDVDLIEYVPWQAMTSIEQNAQLKLDAVDGPFMSLIFNGSRPPFNDPKVRKAVAHAI
ECCCCEEHHHHCCHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHH
RREEIVKSAFFGRGAPLAHLPIPKASPFFNADLAEGWAYNPDLSKKLLAEAGHGGGLSCK
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEE
LLSTAQYGMHKDTAEVVQQHLAAVGINVELNLPDWATRVALGNRGQYDLAVMGTSADSND
EEEHHHCCCCCCHHHHHHHHHHHEECEEEEECCCHHHHHEECCCCCEEEEEEECCCCCCC
PDGLSAYVDGSLSPSYVRSFNLSIPKLTELFQAGREEFDEAKRKAIYHELEQVAIDQVPM
CCCEEEEECCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VGLALRSQGYAMRKDVTGFTNMPGALTFYSGETFETTSVG
HHHHHHCCCCEEHHCCCCCCCCCCEEEEECCCEEECCCCC
>Mature Secondary Structure 
PPPPAFAETMDMADLTRRSVLTGAAAAAMVPLLRIPGAKARTPGILTFGISSFPPSVQP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCC
WANAGTASATVKLALYRGLTSYGPDGKLRGELAESWAPEGKNVWVFKLRDATFSNGDKVT
CCCCCCCHHEEEEEEHHCCHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEE
SADVKWTIEQVAGEKSTAFLRNEMQGVEKIETPDDKTVRLTMKQPSATLPLLMASYHMPI
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCH
MSKASTASQQIGAGPFILKDAERGVSMDLEANPKYYRPGLPKLKGIRVMVYADENLRVAA
HHCCCHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEE
LQSGDVDLIEYVPWQAMTSIEQNAQLKLDAVDGPFMSLIFNGSRPPFNDPKVRKAVAHAI
ECCCCEEHHHHCCHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHH
RREEIVKSAFFGRGAPLAHLPIPKASPFFNADLAEGWAYNPDLSKKLLAEAGHGGGLSCK
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEE
LLSTAQYGMHKDTAEVVQQHLAAVGINVELNLPDWATRVALGNRGQYDLAVMGTSADSND
EEEHHHCCCCCCHHHHHHHHHHHEECEEEEECCCHHHHHEECCCCCEEEEEEECCCCCCC
PDGLSAYVDGSLSPSYVRSFNLSIPKLTELFQAGREEFDEAKRKAIYHELEQVAIDQVPM
CCCEEEEECCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VGLALRSQGYAMRKDVTGFTNMPGALTFYSGETFETTSVG
HHHHHHCCCCEEHHCCCCCCCCCCEEEEECCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA