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The map label for this gene is agaG

Identifier: 158424664

GI number: 158424664

Start: 3472750

End: 3474402

Strand: Direct

Name: agaG

Synonym: AZC_3040

Alternate gene names: NA

Gene position: 3472750-3474402 (Clockwise)

Preceding gene: 158424663

Following gene: 158424665

Centisome position: 64.67

GC content: 68.97

Gene sequence:

>1653_bases
ATGACCCTGAAGCCCTCTCTCGCGCCCTCCGCCGCGCCGGCCCTTGATCCGATCACCGTCGAGGTGATCGGGGCCGCCCT
CTCCTCCATCGTGGAAGAGACCGGCGAGGCGCTGATCCGCGCCTCCTATTCCACCAACATCAAGGAGCGGCGTGACTGCT
CCACGGCGCTGTTCGACGCCGCCGGCAACACGCTCTGCCAGGCCGAGCACATCCCCATCCATCTCGGCAGCTTCATCGGC
ATCGTGCCGCATATCCTGAAGCTGCACCCCACCTCCGAGATGAAGCCGGGCGACGTGTTCGTGGGCAACGACGCCTATGC
GGGCGGCGGCACCCATCTGCCGGACATCGTGCTGGCCGAGCCCATCTTCATCGACGGTGCCATCGCCGCCTGGACGGTGA
ACCTCGCCCACCATTCGGACTTCGCTGACCGCGGCCACGCCCACATCTATCAGGAAGGCCTGCGCATCCCGCCCGTACGG
CTCTATCGCGCCGGCGAGCTGAACAAGGATGTGCAGGATCTCATCCTGCTCAACTGCCAGGTGCCGCATGAGCGCCTGTC
GGACCTGCGCGCCCAGATGGCGGCCAACCGCGTCGGCGTGCAGCGCTTCCAGGCACTCTGCGCCAAGTATGGCACGCAGG
TGGTTCTGGCGGCGGGCGATGCCCTCATGGACTATGCCGAACGCCGCATGCGCGCCGGCATAGCCGCCATTCCGGACGGC
ACCTACCGCTTCGCGGACGTGTTCGACAATCCGGAGATTGACACCACCCTGCCGCTCAGCGTCGAGGTCACCGTCAAGGG
CGATGCCATGACGCTGCATTTCGATGCGCCGGACCAGCTCCGCGCCGGCATCAACATGACCCACACCGCACTGCAGGCGA
CCGCCTATTATGTGGTGAAGTCGGTGGTGGACCCCACGATCCTGCCCAACGCAGGCCTCGCCCGCCCGCTCACCGTCACC
GCCCGGCAGGGCAGCGTGGTGAGCTGCGTGCATCCGGCCGCGGTGAACGGCCGCGTGCAGACCTGCCAGCGCGTGTCCGA
CCTCATCCTCGGCGCCCTCGCCCAGGCGGTGCCGCACAAGGTGACGGCCTGCTCCAACAGTGCCTGCACGGTGGCCACCT
TCATCGGCCAGCGCAAGGACACCGGCGCCATCTGGGTCTACCTCGAGACCATGGGCGGCGGCGGCGGCGCGCGCCATGAC
AAGGACGGCCTCGACGGCGTGCATGTCCATGTCACCAACACCTCCAATCTGCCCATCGAGGCGCTGGAGACCGAATATCC
GCTCACCCTGCTGCGCTACGAGCTGGTGGACGGCTCCGGCGGCGTCGGGCGCTATCGCGGCGGCCTCGGCCTCCGGCGCG
TCTATCGGGCAGACGAGGACTGCCGCGTGCGCGTGGACGTCTCGCGCATTCATTCCCGCAGCTGGGGGCTCGATGGCGGC
GGCGCGGGCGGCAATGGCGGCGTGGAATGCGGCCCCGGCGTGCTGCGCGAGCGCGATGCCCTGACCCTCAAGGCCGGCCA
GTGGTTCGCGGTGGTGAGCCCCGGCGCCGGCGGCTATGGCGCCCCCGCCGAGCGCGACCGCGCGGCAGTGACCCGCGACA
TCGAGGAAGGCGTCATCGACCTTCCGACGGCCCGCGAGATCTACGGCTACTGA

Upstream 100 bases:

>100_bases
GCCCCGCCATCGTCGAGCAGATGGACACCACCACCGTCGTCCCGCCGGGCATGACCGCCCGCGTGGACGCCTATCACAAC
CTCATCCTCGAGGTGGCGGC

Downstream 100 bases:

>100_bases
GCCTCTCCGGCGGCCTCTTTGCCGGCCGCCGCTTCCTTCAAGGCCGCGCGCCCGAGAGGCGCGCCGCATCTTCACGCCGA
CGCCTTTTCTGTCCCATTCC

Product: AgaG protein

Products: ADP; phosphate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTLKPSLAPSAAPALDPITVEVIGAALSSIVEETGEALIRASYSTNIKERRDCSTALFDAAGNTLCQAEHIPIHLGSFIG
IVPHILKLHPTSEMKPGDVFVGNDAYAGGGTHLPDIVLAEPIFIDGAIAAWTVNLAHHSDFADRGHAHIYQEGLRIPPVR
LYRAGELNKDVQDLILLNCQVPHERLSDLRAQMAANRVGVQRFQALCAKYGTQVVLAAGDALMDYAERRMRAGIAAIPDG
TYRFADVFDNPEIDTTLPLSVEVTVKGDAMTLHFDAPDQLRAGINMTHTALQATAYYVVKSVVDPTILPNAGLARPLTVT
ARQGSVVSCVHPAAVNGRVQTCQRVSDLILGALAQAVPHKVTACSNSACTVATFIGQRKDTGAIWVYLETMGGGGGARHD
KDGLDGVHVHVTNTSNLPIEALETEYPLTLLRYELVDGSGGVGRYRGGLGLRRVYRADEDCRVRVDVSRIHSRSWGLDGG
GAGGNGGVECGPGVLRERDALTLKAGQWFAVVSPGAGGYGAPAERDRAAVTRDIEEGVIDLPTAREIYGY

Sequences:

>Translated_550_residues
MTLKPSLAPSAAPALDPITVEVIGAALSSIVEETGEALIRASYSTNIKERRDCSTALFDAAGNTLCQAEHIPIHLGSFIG
IVPHILKLHPTSEMKPGDVFVGNDAYAGGGTHLPDIVLAEPIFIDGAIAAWTVNLAHHSDFADRGHAHIYQEGLRIPPVR
LYRAGELNKDVQDLILLNCQVPHERLSDLRAQMAANRVGVQRFQALCAKYGTQVVLAAGDALMDYAERRMRAGIAAIPDG
TYRFADVFDNPEIDTTLPLSVEVTVKGDAMTLHFDAPDQLRAGINMTHTALQATAYYVVKSVVDPTILPNAGLARPLTVT
ARQGSVVSCVHPAAVNGRVQTCQRVSDLILGALAQAVPHKVTACSNSACTVATFIGQRKDTGAIWVYLETMGGGGGARHD
KDGLDGVHVHVTNTSNLPIEALETEYPLTLLRYELVDGSGGVGRYRGGLGLRRVYRADEDCRVRVDVSRIHSRSWGLDGG
GAGGNGGVECGPGVLRERDALTLKAGQWFAVVSPGAGGYGAPAERDRAAVTRDIEEGVIDLPTAREIYGY
>Mature_549_residues
TLKPSLAPSAAPALDPITVEVIGAALSSIVEETGEALIRASYSTNIKERRDCSTALFDAAGNTLCQAEHIPIHLGSFIGI
VPHILKLHPTSEMKPGDVFVGNDAYAGGGTHLPDIVLAEPIFIDGAIAAWTVNLAHHSDFADRGHAHIYQEGLRIPPVRL
YRAGELNKDVQDLILLNCQVPHERLSDLRAQMAANRVGVQRFQALCAKYGTQVVLAAGDALMDYAERRMRAGIAAIPDGT
YRFADVFDNPEIDTTLPLSVEVTVKGDAMTLHFDAPDQLRAGINMTHTALQATAYYVVKSVVDPTILPNAGLARPLTVTA
RQGSVVSCVHPAAVNGRVQTCQRVSDLILGALAQAVPHKVTACSNSACTVATFIGQRKDTGAIWVYLETMGGGGGARHDK
DGLDGVHVHVTNTSNLPIEALETEYPLTLLRYELVDGSGGVGRYRGGLGLRRVYRADEDCRVRVDVSRIHSRSWGLDGGG
AGGNGGVECGPGVLRERDALTLKAGQWFAVVSPGAGGYGAPAERDRAAVTRDIEEGVIDLPTAREIYGY

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=548, Percent_Identity=32.8467153284672, Blast_Score=189, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI133901900, Length=542, Percent_Identity=28.5977859778598, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI133901902, Length=510, Percent_Identity=27.843137254902, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322634, Length=554, Percent_Identity=29.9638989169675, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI45550492, Length=545, Percent_Identity=29.7247706422018, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.9

Molecular weight: Translated: 58598; Mature: 58467

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKPSLAPSAAPALDPITVEVIGAALSSIVEETGEALIRASYSTNIKERRDCSTALFDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
AGNTLCQAEHIPIHLGSFIGIVPHILKLHPTSEMKPGDVFVGNDAYAGGGTHLPDIVLAE
CCCCEEECCCCCEEHHHHHHHHHHHHEECCCCCCCCCCEEECCCCCCCCCCCCCHHHCCC
PIFIDGAIAAWTVNLAHHSDFADRGHAHIYQEGLRIPPVRLYRAGELNKDVQDLILLNCQ
CEEECCCEEEEEEEEHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCHHHHEEEECC
VPHERLSDLRAQMAANRVGVQRFQALCAKYGTQVVLAAGDALMDYAERRMRAGIAAIPDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCEEECCCC
TYRFADVFDNPEIDTTLPLSVEVTVKGDAMTLHFDAPDQLRAGINMTHTALQATAYYVVK
CEEEEECCCCCCCCEEECEEEEEEEECCEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
SVVDPTILPNAGLARPLTVTARQGSVVSCVHPAAVNGRVQTCQRVSDLILGALAQAVPHK
HHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHCCCH
VTACSNSACTVATFIGQRKDTGAIWVYLETMGGGGGARHDKDGLDGVHVHVTNTSNLPIE
HEECCCCCEEHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHH
ALETEYPLTLLRYELVDGSGGVGRYRGGLGLRRVYRADEDCRVRVDVSRIHSRSWGLDGG
HHCCCCCEEEEEEEEECCCCCCCHHCCCCHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCC
GAGGNGGVECGPGVLRERDALTLKAGQWFAVVSPGAGGYGAPAERDRAAVTRDIEEGVID
CCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
LPTAREIYGY
CCCCHHHCCC
>Mature Secondary Structure 
TLKPSLAPSAAPALDPITVEVIGAALSSIVEETGEALIRASYSTNIKERRDCSTALFDA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
AGNTLCQAEHIPIHLGSFIGIVPHILKLHPTSEMKPGDVFVGNDAYAGGGTHLPDIVLAE
CCCCEEECCCCCEEHHHHHHHHHHHHEECCCCCCCCCCEEECCCCCCCCCCCCCHHHCCC
PIFIDGAIAAWTVNLAHHSDFADRGHAHIYQEGLRIPPVRLYRAGELNKDVQDLILLNCQ
CEEECCCEEEEEEEEHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCHHHHEEEECC
VPHERLSDLRAQMAANRVGVQRFQALCAKYGTQVVLAAGDALMDYAERRMRAGIAAIPDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCEEECCCC
TYRFADVFDNPEIDTTLPLSVEVTVKGDAMTLHFDAPDQLRAGINMTHTALQATAYYVVK
CEEEEECCCCCCCCEEECEEEEEEEECCEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
SVVDPTILPNAGLARPLTVTARQGSVVSCVHPAAVNGRVQTCQRVSDLILGALAQAVPHK
HHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHCCCH
VTACSNSACTVATFIGQRKDTGAIWVYLETMGGGGGARHDKDGLDGVHVHVTNTSNLPIE
HEECCCCCEEHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHH
ALETEYPLTLLRYELVDGSGGVGRYRGGLGLRRVYRADEDCRVRVDVSRIHSRSWGLDGG
HHCCCCCEEEEEEEEECCCCCCCHHCCCCHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCC
GAGGNGGVECGPGVLRERDALTLKAGQWFAVVSPGAGGYGAPAERDRAAVTRDIEEGVID
CCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
LPTAREIYGY
CCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-oxo-L-proline; H2O

Specific reaction: ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]