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The map label for this gene is braC [H]

Identifier: 158424624

GI number: 158424624

Start: 3430445

End: 3431665

Strand: Direct

Name: braC [H]

Synonym: AZC_3000

Alternate gene names: 158424624

Gene position: 3430445-3431665 (Clockwise)

Preceding gene: 158424623

Following gene: 158424625

Centisome position: 63.88

GC content: 66.99

Gene sequence:

>1221_bases
GTGACCCTCTCCCGCCGCCAGACGCTCGCCGGCCTTGCCGGAGCCCTGCTTCTGCCCGCGCTTTCCCGCGAGGCCCTCGC
CCAGACGGCGACCTTCCGCATCGGCGCGCTGAACCCGATCACCGGTGCCGGCGCGCCTTACGGCACCGGCATGCAGAAGA
TGATCCTCGCCGCCGCCGAGATGGTGAATGCGGATGGCGGCGCCGGGGGCCGCAAGCTCGAGGTCTCCGCCGAGGACACC
CAGACCCAGCCGCAGGCGGCGGTTCTGGCCGCCAAGAAGCTCATCGAGGTCAACAAGGTGCAGGCCATCCTCGGCACCTG
GGCCTCGGGCGTTTCGCTGGCCGTCGCCCCGCTCACCAATGACGCCGGCATCCTGCTGATGCACACCTCCGGCGCGCCCG
CCCTCTCCGTGCCGCCGGCCAATGCCAAGGGCCTGCTCTACCGCTTCCAGGCAACCAACGACCGCTTCGGCCGCGCCTTC
GCGGAGATCGCGCAGAAGGAAGGCTTCAAGCGCCCCGCCACCATGGCCTTCAACAACGCCTCGGGCATCGGCAACACCGA
GGGCTTCCGCAAGGCGTGGGAGGCCAAGGGCGGCAAGGTGGTCGAGAACGTCGTCTATGAGCCCAACCAGCCGAGCTATC
GCGCGGAGCTGCAGAAGGTGCTGGCGGCGAAGCCCGACGTGATCGTGATGGGCTCCTATCTCGCCGACACCACCATCATC
CTGCGCGAGTGGTTCCAGACCGGCGAGACCACCAAATTCATCATTCCCGCCTGGGCCGCCAATCCGGACCTCGTGAAGGC
CCTCGGCCCCGAGGTGACGGAAGGCATCATCTCGGTGGACAGCATCTCCAACGAGGGCTCGGACGCCTTCAAGTCGTTCG
ATGCGGCCTACCAGAAGGCCATGGGCCAGTCCGGCCTGAACAATGTCTATGCGGCCATGACCTGGGACATGGTGGTGGTG
CTGGCCCTCGCCATGGAGGCCGCCGGCCCCAATGCGGACGTTGCCGCCATCTCCGCCAAGCTGCGCGAAGTCGCGGGTCC
CGGTGGCAAGACGGTCTCCACCTTCGCCGAAGGCAAGGCGGCGCTGAAGTCCGGCAAGATCGACTACCAGGGCGCCTCCT
CCATCCTGGACTTCGACCAGTATGGCGACGTGACCCCGGACTTCGCGGTGTCCTTCATCAACAAGGGCAAGTTCGACCGC
AAGTATATCGTGAAGCTCTGA

Upstream 100 bases:

>100_bases
GCCAGCACATGCCGCTCATCATGAAGGGCGGCGTCTGCGTGAAGCGTACCGCGCTCAACTGAAAAACAAGCACAACCTCC
AGACCGAAGGGATCGAAGCC

Downstream 100 bases:

>100_bases
CCACGATCCCGAACGTCGCCGGTCCGTTCTGCCGCCGCTCCTCTCCCTCTCCCCGGGCGGCGGCAGGACCGGGCCGGTGA
CGTCCCGCCCGGTCTCCCCG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTLSRRQTLAGLAGALLLPALSREALAQTATFRIGALNPITGAGAPYGTGMQKMILAAAEMVNADGGAGGRKLEVSAEDT
QTQPQAAVLAAKKLIEVNKVQAILGTWASGVSLAVAPLTNDAGILLMHTSGAPALSVPPANAKGLLYRFQATNDRFGRAF
AEIAQKEGFKRPATMAFNNASGIGNTEGFRKAWEAKGGKVVENVVYEPNQPSYRAELQKVLAAKPDVIVMGSYLADTTII
LREWFQTGETTKFIIPAWAANPDLVKALGPEVTEGIISVDSISNEGSDAFKSFDAAYQKAMGQSGLNNVYAAMTWDMVVV
LALAMEAAGPNADVAAISAKLREVAGPGGKTVSTFAEGKAALKSGKIDYQGASSILDFDQYGDVTPDFAVSFINKGKFDR
KYIVKL

Sequences:

>Translated_406_residues
MTLSRRQTLAGLAGALLLPALSREALAQTATFRIGALNPITGAGAPYGTGMQKMILAAAEMVNADGGAGGRKLEVSAEDT
QTQPQAAVLAAKKLIEVNKVQAILGTWASGVSLAVAPLTNDAGILLMHTSGAPALSVPPANAKGLLYRFQATNDRFGRAF
AEIAQKEGFKRPATMAFNNASGIGNTEGFRKAWEAKGGKVVENVVYEPNQPSYRAELQKVLAAKPDVIVMGSYLADTTII
LREWFQTGETTKFIIPAWAANPDLVKALGPEVTEGIISVDSISNEGSDAFKSFDAAYQKAMGQSGLNNVYAAMTWDMVVV
LALAMEAAGPNADVAAISAKLREVAGPGGKTVSTFAEGKAALKSGKIDYQGASSILDFDQYGDVTPDFAVSFINKGKFDR
KYIVKL
>Mature_405_residues
TLSRRQTLAGLAGALLLPALSREALAQTATFRIGALNPITGAGAPYGTGMQKMILAAAEMVNADGGAGGRKLEVSAEDTQ
TQPQAAVLAAKKLIEVNKVQAILGTWASGVSLAVAPLTNDAGILLMHTSGAPALSVPPANAKGLLYRFQATNDRFGRAFA
EIAQKEGFKRPATMAFNNASGIGNTEGFRKAWEAKGGKVVENVVYEPNQPSYRAELQKVLAAKPDVIVMGSYLADTTIIL
REWFQTGETTKFIIPAWAANPDLVKALGPEVTEGIISVDSISNEGSDAFKSFDAAYQKAMGQSGLNNVYAAMTWDMVVVL
ALAMEAAGPNADVAAISAKLREVAGPGGKTVSTFAEGKAALKSGKIDYQGASSILDFDQYGDVTPDFAVSFINKGKFDRK
YIVKL

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42685; Mature: 42554

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSRRQTLAGLAGALLLPALSREALAQTATFRIGALNPITGAGAPYGTGMQKMILAAAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHH
MVNADGGAGGRKLEVSAEDTQTQPQAAVLAAKKLIEVNKVQAILGTWASGVSLAVAPLTN
HHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAGILLMHTSGAPALSVPPANAKGLLYRFQATNDRFGRAFAEIAQKEGFKRPATMAFNNA
CCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCC
SGIGNTEGFRKAWEAKGGKVVENVVYEPNQPSYRAELQKVLAAKPDVIVMGSYLADTTII
CCCCCCHHHHHHHHCCCCCEEHHHEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
LREWFQTGETTKFIIPAWAANPDLVKALGPEVTEGIISVDSISNEGSDAFKSFDAAYQKA
HHHHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
MGQSGLNNVYAAMTWDMVVVLALAMEAAGPNADVAAISAKLREVAGPGGKTVSTFAEGKA
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
ALKSGKIDYQGASSILDFDQYGDVTPDFAVSFINKGKFDRKYIVKL
HHHCCCCCCCCCHHHHCHHHCCCCCHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
TLSRRQTLAGLAGALLLPALSREALAQTATFRIGALNPITGAGAPYGTGMQKMILAAAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHH
MVNADGGAGGRKLEVSAEDTQTQPQAAVLAAKKLIEVNKVQAILGTWASGVSLAVAPLTN
HHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DAGILLMHTSGAPALSVPPANAKGLLYRFQATNDRFGRAFAEIAQKEGFKRPATMAFNNA
CCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCC
SGIGNTEGFRKAWEAKGGKVVENVVYEPNQPSYRAELQKVLAAKPDVIVMGSYLADTTII
CCCCCCHHHHHHHHCCCCCEEHHHEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
LREWFQTGETTKFIIPAWAANPDLVKALGPEVTEGIISVDSISNEGSDAFKSFDAAYQKA
HHHHHHCCCCEEEEEEECCCCHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
MGQSGLNNVYAAMTWDMVVVLALAMEAAGPNADVAAISAKLREVAGPGGKTVSTFAEGKA
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
ALKSGKIDYQGASSILDFDQYGDVTPDFAVSFINKGKFDRKYIVKL
HHHCCCCCCCCCHHHHCHHHCCCCCHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]