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The map label for this gene is braE [H]

Identifier: 158424567

GI number: 158424567

Start: 3365007

End: 3366449

Strand: Direct

Name: braE [H]

Synonym: AZC_2943

Alternate gene names: 158424567

Gene position: 3365007-3366449 (Clockwise)

Preceding gene: 158424566

Following gene: 158424568

Centisome position: 62.67

GC content: 67.84

Gene sequence:

>1443_bases
ATGGCCGCCGCTCCGACCAAGGGCGGGGCCGCCTCTGCGCCCCCCGCCCCGTTTGCCGCCGCGCTGAAGGACGCGGTGGT
GAGCGGTGTCCTCACCTTCCTCCTGCTCCTGCCGCTCGTCGGCTTCCGCGCCACCGAAAGCGGCAGCGGGCCGCTGACGC
TGACCTACCGCTTCGGCCTCGTCACCATCTTCGCCCTGCTGGTGGCGGGCCTGCGGCTGGTGCTCAACCTCACGCTCTGG
CGCGAGGGCCGCGTGAAGGCCACGAAGTCCGGCCCGTCGGCGCTGGCCGTCGTCGGCAACCAGATGGCACCCTTCGCGAA
GAGTGCCTTCTTCGCCATCGCCATCCTCTATCCGCTGCTGGCGGTGCTGCTCTCGGGCGGCCTCACCCCCAGCCGCTACT
GGATCGACCTCGGCATCTATATCGGCTCCTACGTGATGCTGGGCTGGGGCCTCAACATCGTGGTCGGCCTCGCCGGCCTG
CTCGATCTCGGCTACGTGGCCTTCTATGCGGTGGGCGCCTATGCCTTCGCGCTCCTGTCCACGTCCGTACCGATCAATGA
CTGGATGGCCGGCCACCTCGGCGAGAACTTCTGGGCGCTGTGGTCTTTCTGGGTCTGCCTGCCGGTGTGCGGCCTGCTGG
CGGCGCTCTGGGGCGTCATCCTCGGCTTCCCGGTGCTGCGCCTCAGGGGCGACTACCTCGCCATCGTGACGCTGGCCTTC
GGAGAGATCATCCGCCTCGTCCTCATCAACTGGACGGATTTCACCAACGGCGCGGCCGGCATCGCCTCCATCCCGCCCAT
CTCCTTCTTCGGCATTCCCTTTTCGTCCGGAGACGACGGGTTCGCAGCGCTGTTCGGGCTCACCTTCGACCCGATGCACC
GCATCATCTTCCTCTATTTCGTCATCGTGGCGCTCTCGGCCCTCACGGCCCTCGCCACGCTGCGGCTGCGGCGGATGCCG
GTCGGCCGGGCATGGGAGGCGCTGCGCGAGGACGAGATCGCCTGCCGCTCGCTCGGCATCAACACCACGCTCACCAAGCT
CACGGCCTTCGCCACCGGCGCCATGTTCGGCGGCTTTGCGGGCGCCTTCTTCGCGGTGCGCGTGCGGTTCGTTTCGCCCG
AGAGCTTCACCTTCATGGAATCGGCGATGATCCTCGCCATCGTCGTGCTCGGCGGCATGGGCTCGCAGATGGGCGTGGCG
GCGGCGGCCGTCTTCATGATCGGCGGCATGGAGATGCTGCGGAACCTCACCTTCCTGAAGAGCGACTGGCTGCTCGGCCC
CGACTTCGATCCCACCCTCTACCGCATGCTCATCTTCGGCTTCGCCATGGTGGCGGTGATGGTCTGGCGTCCGCGCGGCA
TCGTGTCGGGCCGCCTCGCCACCATCTTCCTGAAGGAGCGCAAGACGGTGTCCGGCGATCTGGTGAAGGAGGGCCACGGA
TGA

Upstream 100 bases:

>100_bases
CCATCGAATACAAGGATGTGGCGGCGTTCTCCATCCTTGCCATCACGCTCATCTTCATGCCTCAGGGGCTCCTCGGCCGT
CCGGAAGTGGAGAAGGTCTG

Downstream 100 bases:

>100_bases
GACGCTGGGAAACCGATCCCATCCTGACCGTGGACCACCTCACCATGCGGTTCGGTGGTCTGGTGGCGGTGAACAACGTG
TCCTTCACCGTGGGACGCGG

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAAAPTKGGAASAPPAPFAAALKDAVVSGVLTFLLLLPLVGFRATESGSGPLTLTYRFGLVTIFALLVAGLRLVLNLTLW
REGRVKATKSGPSALAVVGNQMAPFAKSAFFAIAILYPLLAVLLSGGLTPSRYWIDLGIYIGSYVMLGWGLNIVVGLAGL
LDLGYVAFYAVGAYAFALLSTSVPINDWMAGHLGENFWALWSFWVCLPVCGLLAALWGVILGFPVLRLRGDYLAIVTLAF
GEIIRLVLINWTDFTNGAAGIASIPPISFFGIPFSSGDDGFAALFGLTFDPMHRIIFLYFVIVALSALTALATLRLRRMP
VGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPESFTFMESAMILAIVVLGGMGSQMGVA
AAAVFMIGGMEMLRNLTFLKSDWLLGPDFDPTLYRMLIFGFAMVAVMVWRPRGIVSGRLATIFLKERKTVSGDLVKEGHG

Sequences:

>Translated_480_residues
MAAAPTKGGAASAPPAPFAAALKDAVVSGVLTFLLLLPLVGFRATESGSGPLTLTYRFGLVTIFALLVAGLRLVLNLTLW
REGRVKATKSGPSALAVVGNQMAPFAKSAFFAIAILYPLLAVLLSGGLTPSRYWIDLGIYIGSYVMLGWGLNIVVGLAGL
LDLGYVAFYAVGAYAFALLSTSVPINDWMAGHLGENFWALWSFWVCLPVCGLLAALWGVILGFPVLRLRGDYLAIVTLAF
GEIIRLVLINWTDFTNGAAGIASIPPISFFGIPFSSGDDGFAALFGLTFDPMHRIIFLYFVIVALSALTALATLRLRRMP
VGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPESFTFMESAMILAIVVLGGMGSQMGVA
AAAVFMIGGMEMLRNLTFLKSDWLLGPDFDPTLYRMLIFGFAMVAVMVWRPRGIVSGRLATIFLKERKTVSGDLVKEGHG
>Mature_479_residues
AAAPTKGGAASAPPAPFAAALKDAVVSGVLTFLLLLPLVGFRATESGSGPLTLTYRFGLVTIFALLVAGLRLVLNLTLWR
EGRVKATKSGPSALAVVGNQMAPFAKSAFFAIAILYPLLAVLLSGGLTPSRYWIDLGIYIGSYVMLGWGLNIVVGLAGLL
DLGYVAFYAVGAYAFALLSTSVPINDWMAGHLGENFWALWSFWVCLPVCGLLAALWGVILGFPVLRLRGDYLAIVTLAFG
EIIRLVLINWTDFTNGAAGIASIPPISFFGIPFSSGDDGFAALFGLTFDPMHRIIFLYFVIVALSALTALATLRLRRMPV
GRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFAGAFFAVRVRFVSPESFTFMESAMILAIVVLGGMGSQMGVAA
AAVFMIGGMEMLRNLTFLKSDWLLGPDFDPTLYRMLIFGFAMVAVMVWRPRGIVSGRLATIFLKERKTVSGDLVKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=341, Percent_Identity=50.733137829912, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 51335; Mature: 51203

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAPTKGGAASAPPAPFAAALKDAVVSGVLTFLLLLPLVGFRATESGSGPLTLTYRFGL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHH
VTIFALLVAGLRLVLNLTLWREGRVKATKSGPSALAVVGNQMAPFAKSAFFAIAILYPLL
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
AVLLSGGLTPSRYWIDLGIYIGSYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYAFALLS
HHHHHCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSVPINDWMAGHLGENFWALWSFWVCLPVCGLLAALWGVILGFPVLRLRGDYLAIVTLAF
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
GEIIRLVLINWTDFTNGAAGIASIPPISFFGIPFSSGDDGFAALFGLTFDPMHRIIFLYF
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHH
VIVALSALTALATLRLRRMPVGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GAFFAVRVRFVSPESFTFMESAMILAIVVLGGMGSQMGVAAAAVFMIGGMEMLRNLTFLK
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDWLLGPDFDPTLYRMLIFGFAMVAVMVWRPRGIVSGRLATIFLKERKTVSGDLVKEGHG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAAPTKGGAASAPPAPFAAALKDAVVSGVLTFLLLLPLVGFRATESGSGPLTLTYRFGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHH
VTIFALLVAGLRLVLNLTLWREGRVKATKSGPSALAVVGNQMAPFAKSAFFAIAILYPLL
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
AVLLSGGLTPSRYWIDLGIYIGSYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYAFALLS
HHHHHCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSVPINDWMAGHLGENFWALWSFWVCLPVCGLLAALWGVILGFPVLRLRGDYLAIVTLAF
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
GEIIRLVLINWTDFTNGAAGIASIPPISFFGIPFSSGDDGFAALFGLTFDPMHRIIFLYF
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHH
VIVALSALTALATLRLRRMPVGRAWEALREDEIACRSLGINTTLTKLTAFATGAMFGGFA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GAFFAVRVRFVSPESFTFMESAMILAIVVLGGMGSQMGVAAAAVFMIGGMEMLRNLTFLK
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDWLLGPDFDPTLYRMLIFGFAMVAVMVWRPRGIVSGRLATIFLKERKTVSGDLVKEGHG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]