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The map label for this gene is braF [H]

Identifier: 158424568

GI number: 158424568

Start: 3366446

End: 3367357

Strand: Direct

Name: braF [H]

Synonym: AZC_2944

Alternate gene names: 158424568

Gene position: 3366446-3367357 (Clockwise)

Preceding gene: 158424567

Following gene: 158424569

Centisome position: 62.69

GC content: 67.32

Gene sequence:

>912_bases
ATGAGACGCTGGGAAACCGATCCCATCCTGACCGTGGACCACCTCACCATGCGGTTCGGTGGTCTGGTGGCGGTGAACAA
CGTGTCCTTCACCGTGGGACGCGGCGACGTGACCGCCCTCATCGGGCCGAACGGCGCGGGCAAGACCACCGCCTTCAACT
GCATCACCGGCTTCTACAAGCCGACGCTGGGGCGGCTCGCCCTCGCCCACGAGGCGCCGCCGAGCCCGGCGGAGCTCCAG
GCGCTGACCGCCAACGGCCAGGCCGGGGCGAAGACCGAGAACGGTGCGCTCTATCTCCTGGAGCGCCTGCCGGACCACAA
GGTCTCGGCTCAGGCGCGGGTCGCCCGCACCTTCCAGAACATCCGCCTGTTCTCAGGCATGACGGTGCTGGAAAACCTCA
TGGTGGCGCAGCACCAGAAGCTGATCCGCTCCGGCATGATGGCGCCCTCCGCCCTCTTCAACCTGCCCTCCTGGCGCCGC
CAGGAGAAGGAAGGCGTGGAGCGGGCGCTGCACTGGCTCGAAAAGGTGGACCTGATGGCCCGCGCCGACGATCCGGCCGG
CGACCTGCCCTATGGCGACCAGCGGCGGCTGGAGATCGCCCGTGCCATGTGCACCGAGCCCGTCCTGCTCTGCCTCGACG
AGCCCGCCGCCGGCCTCAATCCGAAGGAATCAGCCGCGCTCAACACGCTGCTCCTGTCCATCCGCGCCGATTACGGCACC
TCGGTGCTGCTGATCGAGCATGACATGTCGGTGGTGATGGAAATCTCGGACCATGTGATCGTGCTGGAATACGGCAGCAA
GATCTCCGACGGCACACCGGCGGAGGTACGCTCCGATCCCGCCGTCATCGCCGCTTATCTCGGCGTGGACGAGGATGCGG
TCGAGGCCGTGGAAGCGGAGGTGGGCCTATGA

Upstream 100 bases:

>100_bases
TGGTCTGGCGTCCGCGCGGCATCGTGTCGGGCCGCCTCGCCACCATCTTCCTGAAGGAGCGCAAGACGGTGTCCGGCGAT
CTGGTGAAGGAGGGCCACGG

Downstream 100 bases:

>100_bases
CGGCGCTGCTCTCCGTGAAGGGGGTCAAGACCTATTACGGGAACATCGTCGCGCTGAAGGGCGTGGACGTGGAAGTGAAC
GAGGGCGAGATCGTCACCCT

Product: ABC transporter ATPase

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRRWETDPILTVDHLTMRFGGLVAVNNVSFTVGRGDVTALIGPNGAGKTTAFNCITGFYKPTLGRLALAHEAPPSPAELQ
ALTANGQAGAKTENGALYLLERLPDHKVSAQARVARTFQNIRLFSGMTVLENLMVAQHQKLIRSGMMAPSALFNLPSWRR
QEKEGVERALHWLEKVDLMARADDPAGDLPYGDQRRLEIARAMCTEPVLLCLDEPAAGLNPKESAALNTLLLSIRADYGT
SVLLIEHDMSVVMEISDHVIVLEYGSKISDGTPAEVRSDPAVIAAYLGVDEDAVEAVEAEVGL

Sequences:

>Translated_303_residues
MRRWETDPILTVDHLTMRFGGLVAVNNVSFTVGRGDVTALIGPNGAGKTTAFNCITGFYKPTLGRLALAHEAPPSPAELQ
ALTANGQAGAKTENGALYLLERLPDHKVSAQARVARTFQNIRLFSGMTVLENLMVAQHQKLIRSGMMAPSALFNLPSWRR
QEKEGVERALHWLEKVDLMARADDPAGDLPYGDQRRLEIARAMCTEPVLLCLDEPAAGLNPKESAALNTLLLSIRADYGT
SVLLIEHDMSVVMEISDHVIVLEYGSKISDGTPAEVRSDPAVIAAYLGVDEDAVEAVEAEVGL
>Mature_303_residues
MRRWETDPILTVDHLTMRFGGLVAVNNVSFTVGRGDVTALIGPNGAGKTTAFNCITGFYKPTLGRLALAHEAPPSPAELQ
ALTANGQAGAKTENGALYLLERLPDHKVSAQARVARTFQNIRLFSGMTVLENLMVAQHQKLIRSGMMAPSALFNLPSWRR
QEKEGVERALHWLEKVDLMARADDPAGDLPYGDQRRLEIARAMCTEPVLLCLDEPAAGLNPKESAALNTLLLSIRADYGT
SVLLIEHDMSVVMEISDHVIVLEYGSKISDGTPAEVRSDPAVIAAYLGVDEDAVEAVEAEVGL

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1789864, Length=283, Percent_Identity=48.0565371024735, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1789672, Length=276, Percent_Identity=25, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790525, Length=275, Percent_Identity=26.9090909090909, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789593, Length=283, Percent_Identity=28.6219081272085, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786398, Length=285, Percent_Identity=28.7719298245614, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48995001, Length=258, Percent_Identity=28.2945736434109, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=282, Percent_Identity=29.4326241134752, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788225, Length=273, Percent_Identity=26.3736263736264, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081709, Length=277, Percent_Identity=24.187725631769, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787370, Length=284, Percent_Identity=25.3521126760563, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786872, Length=274, Percent_Identity=24.0875912408759, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI48994997, Length=276, Percent_Identity=26.4492753623188, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081782, Length=276, Percent_Identity=27.536231884058, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789991, Length=274, Percent_Identity=25.9124087591241, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082268, Length=284, Percent_Identity=26.056338028169, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787029, Length=272, Percent_Identity=23.5294117647059, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788210, Length=286, Percent_Identity=25.1748251748252, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788761, Length=273, Percent_Identity=24.5421245421245, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081791, Length=282, Percent_Identity=23.758865248227, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789032, Length=284, Percent_Identity=24.6478873239437, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790544, Length=273, Percent_Identity=24.1758241758242, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790190, Length=277, Percent_Identity=24.5487364620939, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788450, Length=289, Percent_Identity=23.1833910034602, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790162, Length=287, Percent_Identity=25.4355400696864, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788897, Length=286, Percent_Identity=26.5734265734266, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786253, Length=290, Percent_Identity=26.8965517241379, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081834, Length=252, Percent_Identity=26.1904761904762, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787712, Length=278, Percent_Identity=23.3812949640288, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI48994883, Length=294, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789962, Length=258, Percent_Identity=24.8062015503876, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787500, Length=267, Percent_Identity=26.9662921348315, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787089, Length=255, Percent_Identity=23.5294117647059, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789586, Length=261, Percent_Identity=25.6704980842912, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786345, Length=278, Percent_Identity=25.8992805755396, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788506, Length=262, Percent_Identity=27.8625954198473, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789891, Length=256, Percent_Identity=25.78125, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790467, Length=281, Percent_Identity=20.9964412811388, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788053, Length=244, Percent_Identity=26.6393442622951, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787758, Length=176, Percent_Identity=28.4090909090909, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789873, Length=258, Percent_Identity=24.4186046511628, Blast_Score=61, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24643674, Length=277, Percent_Identity=24.5487364620939, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32811; Mature: 32811

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRWETDPILTVDHLTMRFGGLVAVNNVSFTVGRGDVTALIGPNGAGKTTAFNCITGFYK
CCCCCCCCEEEHHHHHHHHCCEEEECCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
PTLGRLALAHEAPPSPAELQALTANGQAGAKTENGALYLLERLPDHKVSAQARVARTFQN
HHHHHEEEECCCCCCCHHEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IRLFSGMTVLENLMVAQHQKLIRSGMMAPSALFNLPSWRRQEKEGVERALHWLEKVDLMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RADDPAGDLPYGDQRRLEIARAMCTEPVLLCLDEPAAGLNPKESAALNTLLLSIRADYGT
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCC
SVLLIEHDMSVVMEISDHVIVLEYGSKISDGTPAEVRSDPAVIAAYLGVDEDAVEAVEAE
EEEEEECCCEEEEEECCCEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHH
VGL
CCC
>Mature Secondary Structure
MRRWETDPILTVDHLTMRFGGLVAVNNVSFTVGRGDVTALIGPNGAGKTTAFNCITGFYK
CCCCCCCCEEEHHHHHHHHCCEEEECCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
PTLGRLALAHEAPPSPAELQALTANGQAGAKTENGALYLLERLPDHKVSAQARVARTFQN
HHHHHEEEECCCCCCCHHEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IRLFSGMTVLENLMVAQHQKLIRSGMMAPSALFNLPSWRRQEKEGVERALHWLEKVDLMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RADDPAGDLPYGDQRRLEIARAMCTEPVLLCLDEPAAGLNPKESAALNTLLLSIRADYGT
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHEEECCCCC
SVLLIEHDMSVVMEISDHVIVLEYGSKISDGTPAEVRSDPAVIAAYLGVDEDAVEAVEAE
EEEEEECCCEEEEEECCCEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHH
VGL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]