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The map label for this gene is yfcG [H]

Identifier: 158424551

GI number: 158424551

Start: 3348103

End: 3348807

Strand: Reverse

Name: yfcG [H]

Synonym: AZC_2927

Alternate gene names: 158424551

Gene position: 3348807-3348103 (Counterclockwise)

Preceding gene: 158424552

Following gene: 158424550

Centisome position: 62.36

GC content: 63.97

Gene sequence:

>705_bases
ATGGCGGACGCTCAGCAACAGCCGATCGAGCTGCTGTATTGGCCGACACCGAACGGATGGAAAGTCTCCATCATGCTTGA
GGAGTGCGGGCTGCCGTACGAGATCAAGCTCGTGAACATCGGCAAGGGCGACCAGTTCGCGCCCGATTTCCTCGCCGTGT
CGCCGAACAACAAGATCCCCGCCATCATCGATCCGAACGGGCCGGGCGGCACGCCCATCTCGGTCTTCGAGAGCGGCGCC
ATCCTGCAGTATCTCGGCCGCAAGACCGGCAAGTTCTATCCCACCGACGAGCGCAAGCGCGTCGAAGTGGAGGAATGGCT
GTACTGGCAGGTGAGCGGCCTCGGCCCCATGGCTGGCCAGGCGCATCACTTCCGCCTCTACGCGCCCGAGAAGATTCCCT
ATGCGGTCTCGCGCTACACGAACGAGGTGCACCGCCTCTATGGCGTGATGAACAAGCGCCTCGAAGATCGTGAATTTCTC
GCCGGCGACTTCTCCATTGCCGACATCGCCTGCATCGGCTGGGCGAAGCTCTGGGAGCGGCAGGGACAGGACATTGCCGA
ATTCCCTCATCTCGGCCGCTGGATCGAGACCGTGAAGGCCCGCCCCGGCGTGCAGCGCGGCCTTGCGGTGAATGCCGAGC
AGAAGACGGACCTCTCCAAGGACAAGGCGGCCCAGTCGGTGCTGTTCGGCCAGCGGGCCCGCTGA

Upstream 100 bases:

>100_bases
TCCGTTAACGAACTGTTAACAGTCCCGCTTGCCCCCGGCGCGCGCTCGGCCTACCTGTAGCGGCATTCGCCGGCGGGCCG
GTCGAGGATGGAGCACTGGA

Downstream 100 bases:

>100_bases
TCGCAGGAGAGGCCGGGTGGCAAGCGGGTCGGAGAAGCCGCCCCTCTGGCTTCGGGCGATGGTTTCTGGCATGTGCGACG
AAACGGGGCGCCGGTGACGG

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MADAQQQPIELLYWPTPNGWKVSIMLEECGLPYEIKLVNIGKGDQFAPDFLAVSPNNKIPAIIDPNGPGGTPISVFESGA
ILQYLGRKTGKFYPTDERKRVEVEEWLYWQVSGLGPMAGQAHHFRLYAPEKIPYAVSRYTNEVHRLYGVMNKRLEDREFL
AGDFSIADIACIGWAKLWERQGQDIAEFPHLGRWIETVKARPGVQRGLAVNAEQKTDLSKDKAAQSVLFGQRAR

Sequences:

>Translated_234_residues
MADAQQQPIELLYWPTPNGWKVSIMLEECGLPYEIKLVNIGKGDQFAPDFLAVSPNNKIPAIIDPNGPGGTPISVFESGA
ILQYLGRKTGKFYPTDERKRVEVEEWLYWQVSGLGPMAGQAHHFRLYAPEKIPYAVSRYTNEVHRLYGVMNKRLEDREFL
AGDFSIADIACIGWAKLWERQGQDIAEFPHLGRWIETVKARPGVQRGLAVNAEQKTDLSKDKAAQSVLFGQRAR
>Mature_233_residues
ADAQQQPIELLYWPTPNGWKVSIMLEECGLPYEIKLVNIGKGDQFAPDFLAVSPNNKIPAIIDPNGPGGTPISVFESGAI
LQYLGRKTGKFYPTDERKRVEVEEWLYWQVSGLGPMAGQAHHFRLYAPEKIPYAVSRYTNEVHRLYGVMNKRLEDREFLA
GDFSIADIACIGWAKLWERQGQDIAEFPHLGRWIETVKARPGVQRGLAVNAEQKTDLSKDKAAQSVLFGQRAR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=202, Percent_Identity=51.980198019802, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI87082195, Length=221, Percent_Identity=44.3438914027149, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324100, Length=248, Percent_Identity=34.2741935483871, Blast_Score=135, Evalue=4e-33,
Organism=Drosophila melanogaster, GI28571670, Length=175, Percent_Identity=29.7142857142857, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI17933730, Length=224, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17864598, Length=203, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17864592, Length=203, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45549270, Length=201, Percent_Identity=28.3582089552239, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654975, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI85725204, Length=201, Percent_Identity=27.8606965174129, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737923, Length=201, Percent_Identity=27.8606965174129, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17864594, Length=198, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221378983, Length=225, Percent_Identity=27.5555555555556, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646259, Length=225, Percent_Identity=27.5555555555556, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646249, Length=197, Percent_Identity=25.3807106598985, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864596, Length=199, Percent_Identity=25.1256281407035, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646251, Length=174, Percent_Identity=24.7126436781609, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26386; Mature: 26255

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAQQQPIELLYWPTPNGWKVSIMLEECGLPYEIKLVNIGKGDQFAPDFLAVSPNNKIP
CCCCCCCCEEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCC
AIIDPNGPGGTPISVFESGAILQYLGRKTGKFYPTDERKRVEVEEWLYWQVSGLGPMAGQ
EEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHEEEEEECCCCCCCCC
AHHFRLYAPEKIPYAVSRYTNEVHRLYGVMNKRLEDREFLAGDFSIADIACIGWAKLWER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHH
QGQDIAEFPHLGRWIETVKARPGVQRGLAVNAEQKTDLSKDKAAQSVLFGQRAR
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADAQQQPIELLYWPTPNGWKVSIMLEECGLPYEIKLVNIGKGDQFAPDFLAVSPNNKIP
CCCCCCCEEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCC
AIIDPNGPGGTPISVFESGAILQYLGRKTGKFYPTDERKRVEVEEWLYWQVSGLGPMAGQ
EEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHEEEEEECCCCCCCCC
AHHFRLYAPEKIPYAVSRYTNEVHRLYGVMNKRLEDREFLAGDFSIADIACIGWAKLWER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHH
QGQDIAEFPHLGRWIETVKARPGVQRGLAVNAEQKTDLSKDKAAQSVLFGQRAR
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]