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The map label for this gene is fadA [H]

Identifier: 158424499

GI number: 158424499

Start: 3274845

End: 3276032

Strand: Direct

Name: fadA [H]

Synonym: AZC_2875

Alternate gene names: 158424499

Gene position: 3274845-3276032 (Clockwise)

Preceding gene: 158424498

Following gene: 158424500

Centisome position: 60.99

GC content: 69.19

Gene sequence:

>1188_bases
ATGGTCGATGCCGTCATCGTCTCCACCGCCCGCACGCCCATCGGCAAGGCGCAGCGCGGCGCGTTCAACATCACCAAGGG
CGCAGACATGGCCGCCCACGCCATCCGCCACGCGCTTGCAAAGGGCAAGGTGGACCCGGCCAGCGTGGAAGAGGTGGTTC
TCGGCTGCGGTTATCCCGAAGGCGCCACGGGCGGAAACGTCGCCCGCCATGCCTCGCTGATCGCGGGCATACCGGTTACC
GCCTCCGGCGCCACCGTGAGCCGCTTCTGTGCCTCCGGCCTTGAGGCCATTGCGGGCGCGGCCCGGCGCATCGTCATGGA
TGGCGTGCCGGTGGCGGTCGCCGGCGGCGTGGAAAGCATCTCGCTGGTGCAACCCGTCAATCGCGAGCTGTTCCGCAACG
ACTGGCTGATGGCCAACAGGCCGGACATCTACGTCACCATGATCGAGACCGGCGACACGGTGGCCCGCCGCTACGGCGTC
TCGCGCGAGGCGCAGGACCAGTTCGCCCTTGCCAGCCAGCAGCGCACCGCCCGTGCGCAGGCTGGCGGCCTGTTCGATGA
CGAGATCGTCCCCATGACGGTCGAGAAGGAAATCACCGACAAGGCGAGCGGCGAGACGCGCCGCGAGAGCGTGACCCTCG
CCAAGGATGAAGGCAACCGCGCGGACACGACGCTGGAGGGCCTCGCCAAGCTGAAGCCGGTGCGCGGCGACGACTGGTAT
GTCACCGCCGGCAATGCGAGCCAGCTCTCGGACGGCGCCTCGGCCACCGTTCTCATGTCCTCCACCGAGGCCGCGCGGCA
GAATGCGCCCGTATTGGGCATCTTCCGCGGCTATGCCACCGCCGGCTGCGAGCCGGACGAGATGGGCATCGGCCCGGTGT
TCGCCGTGCCGCGTCTGCTGGAACGGCAGGGCCTGAAGGTCTCCGACATCGGCCTGTGGGAGCTGAACGAGGCCTTTGCG
TCCCAGTCCCTTTACTGCCGGGACACGCTCGGCCTCGACCCCGAGATCGTGAACGTGAACGGCGGCGCCATTTCCGTCGG
CCATCCCTTCGGCATGAGCGGCGCCCGGCTCGTCGGCCATGCGGTGCTGGAAGGCCGGCGCCGGGGCGTGCGCTACGCGG
TCGTCACCATGTGCATCGCCGGCGGCATGGGCGCAGCCGGCCTCATCGAAATCAACCCGGACGCCTAA

Upstream 100 bases:

>100_bases
GTCGGCAAGCTGCTGCCCAAGGTGCTGATCGAGGAAGTGGCCGCGCAGGCGCCCGCCCCGGCGGCGGGCCACCCATAAGA
TACCGGAGGATACACCCCAG

Downstream 100 bases:

>100_bases
TCCATGGACCTGCGCTTCACCCCCGAAGAGATCGCCTTCCGCGAGGAGGTGCGCACCTTCTTCCGCACCGAGATTCCGGC
GGAAATCCGCGCCAAGGTTT

Product: thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVDAVIVSTARTPIGKAQRGAFNITKGADMAAHAIRHALAKGKVDPASVEEVVLGCGYPEGATGGNVARHASLIAGIPVT
ASGATVSRFCASGLEAIAGAARRIVMDGVPVAVAGGVESISLVQPVNRELFRNDWLMANRPDIYVTMIETGDTVARRYGV
SREAQDQFALASQQRTARAQAGGLFDDEIVPMTVEKEITDKASGETRRESVTLAKDEGNRADTTLEGLAKLKPVRGDDWY
VTAGNASQLSDGASATVLMSSTEAARQNAPVLGIFRGYATAGCEPDEMGIGPVFAVPRLLERQGLKVSDIGLWELNEAFA
SQSLYCRDTLGLDPEIVNVNGGAISVGHPFGMSGARLVGHAVLEGRRRGVRYAVVTMCIAGGMGAAGLIEINPDA

Sequences:

>Translated_395_residues
MVDAVIVSTARTPIGKAQRGAFNITKGADMAAHAIRHALAKGKVDPASVEEVVLGCGYPEGATGGNVARHASLIAGIPVT
ASGATVSRFCASGLEAIAGAARRIVMDGVPVAVAGGVESISLVQPVNRELFRNDWLMANRPDIYVTMIETGDTVARRYGV
SREAQDQFALASQQRTARAQAGGLFDDEIVPMTVEKEITDKASGETRRESVTLAKDEGNRADTTLEGLAKLKPVRGDDWY
VTAGNASQLSDGASATVLMSSTEAARQNAPVLGIFRGYATAGCEPDEMGIGPVFAVPRLLERQGLKVSDIGLWELNEAFA
SQSLYCRDTLGLDPEIVNVNGGAISVGHPFGMSGARLVGHAVLEGRRRGVRYAVVTMCIAGGMGAAGLIEINPDA
>Mature_395_residues
MVDAVIVSTARTPIGKAQRGAFNITKGADMAAHAIRHALAKGKVDPASVEEVVLGCGYPEGATGGNVARHASLIAGIPVT
ASGATVSRFCASGLEAIAGAARRIVMDGVPVAVAGGVESISLVQPVNRELFRNDWLMANRPDIYVTMIETGDTVARRYGV
SREAQDQFALASQQRTARAQAGGLFDDEIVPMTVEKEITDKASGETRRESVTLAKDEGNRADTTLEGLAKLKPVRGDDWY
VTAGNASQLSDGASATVLMSSTEAARQNAPVLGIFRGYATAGCEPDEMGIGPVFAVPRLLERQGLKVSDIGLWELNEAFA
SQSLYCRDTLGLDPEIVNVNGGAISVGHPFGMSGARLVGHAVLEGRRRGVRYAVVTMCIAGGMGAAGLIEINPDA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=43.5897435897436, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=37.5314861460957, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=35.8395989974937, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=35.2357320099256, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4504327, Length=438, Percent_Identity=31.5068493150685, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194353979, Length=242, Percent_Identity=36.3636363636364, Blast_Score=121, Evalue=9e-28,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=42.4757281553398, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082165, Length=404, Percent_Identity=37.8712871287129, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=37.7777777777778, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI48994986, Length=395, Percent_Identity=36.9620253164557, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788683, Length=420, Percent_Identity=30.952380952381, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=36.7758186397985, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=33.6683417085427, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=35.75, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=439, Percent_Identity=29.8405466970387, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=401, Percent_Identity=28.6783042394015, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322031, Length=398, Percent_Identity=35.929648241206, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6325229, Length=413, Percent_Identity=32.4455205811138, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655093, Length=403, Percent_Identity=39.2059553349876, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17648125, Length=355, Percent_Identity=36.6197183098592, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=32.8395061728395, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137578, Length=442, Percent_Identity=31.447963800905, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41291; Mature: 41291

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDAVIVSTARTPIGKAQRGAFNITKGADMAAHAIRHALAKGKVDPASVEEVVLGCGYPE
CCCEEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCC
GATGGNVARHASLIAGIPVTASGATVSRFCASGLEAIAGAARRIVMDGVPVAVAGGVESI
CCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
SLVQPVNRELFRNDWLMANRPDIYVTMIETGDTVARRYGVSREAQDQFALASQQRTARAQ
HHHHHHHHHHHHCCCEEECCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AGGLFDDEIVPMTVEKEITDKASGETRRESVTLAKDEGNRADTTLEGLAKLKPVRGDDWY
CCCCCCCCEEEEEECHHHHCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCEE
VTAGNASQLSDGASATVLMSSTEAARQNAPVLGIFRGYATAGCEPDEMGIGPVFAVPRLL
EECCCHHHCCCCCCEEEEEECCHHHHHCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHH
ERQGLKVSDIGLWELNEAFASQSLYCRDTLGLDPEIVNVNGGAISVGHPFGMSGARLVGH
HHCCCEEECCCHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEECCCCCCCCHHHHHH
AVLEGRRRGVRYAVVTMCIAGGMGAAGLIEINPDA
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MVDAVIVSTARTPIGKAQRGAFNITKGADMAAHAIRHALAKGKVDPASVEEVVLGCGYPE
CCCEEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCC
GATGGNVARHASLIAGIPVTASGATVSRFCASGLEAIAGAARRIVMDGVPVAVAGGVESI
CCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
SLVQPVNRELFRNDWLMANRPDIYVTMIETGDTVARRYGVSREAQDQFALASQQRTARAQ
HHHHHHHHHHHHCCCEEECCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AGGLFDDEIVPMTVEKEITDKASGETRRESVTLAKDEGNRADTTLEGLAKLKPVRGDDWY
CCCCCCCCEEEEEECHHHHCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHHCCCCCCCEE
VTAGNASQLSDGASATVLMSSTEAARQNAPVLGIFRGYATAGCEPDEMGIGPVFAVPRLL
EECCCHHHCCCCCCEEEEEECCHHHHHCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHH
ERQGLKVSDIGLWELNEAFASQSLYCRDTLGLDPEIVNVNGGAISVGHPFGMSGARLVGH
HHCCCEEECCCHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEECCCCCCCCHHHHHH
AVLEGRRRGVRYAVVTMCIAGGMGAAGLIEINPDA
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]