Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 158424468

GI number: 158424468

Start: 3237602

End: 3239083

Strand: Direct

Name: gatA

Synonym: AZC_2844

Alternate gene names: 158424468

Gene position: 3237602-3239083 (Clockwise)

Preceding gene: 158424467

Following gene: 158424469

Centisome position: 60.29

GC content: 68.76

Gene sequence:

>1482_bases
ATGACCGCGCTCAACCAGCTCACCCTCACCGAAGCGCGCGAGGGCCTCGCCACCGGCGAGTTCACGGCGGTCGAACTGAC
GCAGTCCTATCTCGACGCCATGGCCGAGGCCCGCACCCTCAACGCCTATGTGCTGGAGACCGCCGACAAGGCGCTCGCCA
TGGCCGCCGAAAGCGACAAGCGCATTGCCTCGGGAGATGCCGGCAAGCTGGAAGGCCTGCCCATCGGCGTGAAGGACATG
TTCTGCACCGACGGCGTGCGCACCACGGCCGGCTCCAAGATCCTCGGCGATTTCGTGCCGCCCTATGAATCCACCGTCAC
CAGCCAGCTCTGGCGCGACGGCGCGGTGATGCTGGGCAAGCTCAACAATGACGAGTTCGCCATGGGCTCGTCCAACGAGA
CATCGGCGCAGGGTCCGGTGGTGTCGCCCTGGCGGCGCGAGGGCGATTCCGCCCCGCTGGTGCCCGGCGGCTCGTCCGGC
GGCTCGGCGGCGGCGGTGGCCGCCTTCCTGTGCGCGGGCGCGACCGGCACCGACACCGGCGGCTCCATCCGCCAGCCCGC
CGCCTTCACCGGCACGGTGGGCATCAAGCCCACCTACGGCCGTTGCTCGCGCTTCGGCATCATCGCCTATGCCTCGTCGC
TGGATCAGGCCGGGCCCTTTGCCCGCACGGTGAATGACGCCGCCGTGCTGCTGCGCTCCATGGCCGGCTATGACCCGAAG
GATTCCACCAGCGTGAACCGGTCCGTGCCGGATTACGAGGATGCGGTGGGTGCCTCCATCAAGGGCAAGCGCATCGGCAT
CCCGCGCGAATATCGCGTGGAGGGTCTCGATCCCGAGATCGCCGGCATGTGGGAAGAAGGCGCCCAGTGGCTGCGCGATG
CGGGCGCGGAGGTGGTGGACATCTCGCTGCCCCACACGAAGTACGCCCTGCCCACCTATTACATCGTGGCGCCGGCCGAG
GCCTCCTCGAACCTCGCCCGCTATGACGGCGTGCGCTACGGCACCCGCGTCCCCGGCCGCGACATCATCGACATGTACGA
GCGCACCCGCGCCGCCGGCTTCGGGCCGGAGGTGCGTCGCCGCATCATGATCGGCACCTATGTGCTCTCGGCCGGCTATT
ACGACGCCTATTACGTGAAGGCCCAGAAGGTCCGCACCCTCATCAAGCGCGACTTCGACGACGTGTTCGCGCAGGGCGTG
GATGCGGTGCTCACCCCCGCCACCCCCTCGCCCGCCTTCGGCATCGGCGAGAAGGGCAGCGCGGACCCGGTGGAGATGTA
TCTCCAGGACATCTTCACCGTCACCGTGAACCTTGCCGGCCTGCCGGGCATCTCCGTGCCCGCCGGCCTCTCCTGCAAGA
ACCTGCCGCTCGGCCTCCAGCTCATCGGCCGCCCGTTCGAGGAAGAGACCCTCTTCTCCCTCGCGCAGGTGGTGGAAGAG
GCCGCCGGCCGCTTCCCCGTCGACGACCTGTGGTGGAAGTGA

Upstream 100 bases:

>100_bases
TGCGCGAGGACGTGGTCACCGACGGCCACTATCCCGAGAAGATCCTCGCCAATGCCCCGGAAGCCGAAGGCGGCTTCTTC
ACTGTTCCGAAGGTGGTGGA

Downstream 100 bases:

>100_bases
TCTCCGAACAGGAGGCGGCCGCGCGCCGCCTCCTGAAGCACCTGCGGGGCGAGATCGACGCCATCGACGACCGGCTTGTC
GATCTGCTCACCACCCGCCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTALNQLTLTEAREGLATGEFTAVELTQSYLDAMAEARTLNAYVLETADKALAMAAESDKRIASGDAGKLEGLPIGVKDM
FCTDGVRTTAGSKILGDFVPPYESTVTSQLWRDGAVMLGKLNNDEFAMGSSNETSAQGPVVSPWRREGDSAPLVPGGSSG
GSAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIIAYASSLDQAGPFARTVNDAAVLLRSMAGYDPK
DSTSVNRSVPDYEDAVGASIKGKRIGIPREYRVEGLDPEIAGMWEEGAQWLRDAGAEVVDISLPHTKYALPTYYIVAPAE
ASSNLARYDGVRYGTRVPGRDIIDMYERTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYVKAQKVRTLIKRDFDDVFAQGV
DAVLTPATPSPAFGIGEKGSADPVEMYLQDIFTVTVNLAGLPGISVPAGLSCKNLPLGLQLIGRPFEEETLFSLAQVVEE
AAGRFPVDDLWWK

Sequences:

>Translated_493_residues
MTALNQLTLTEAREGLATGEFTAVELTQSYLDAMAEARTLNAYVLETADKALAMAAESDKRIASGDAGKLEGLPIGVKDM
FCTDGVRTTAGSKILGDFVPPYESTVTSQLWRDGAVMLGKLNNDEFAMGSSNETSAQGPVVSPWRREGDSAPLVPGGSSG
GSAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIIAYASSLDQAGPFARTVNDAAVLLRSMAGYDPK
DSTSVNRSVPDYEDAVGASIKGKRIGIPREYRVEGLDPEIAGMWEEGAQWLRDAGAEVVDISLPHTKYALPTYYIVAPAE
ASSNLARYDGVRYGTRVPGRDIIDMYERTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYVKAQKVRTLIKRDFDDVFAQGV
DAVLTPATPSPAFGIGEKGSADPVEMYLQDIFTVTVNLAGLPGISVPAGLSCKNLPLGLQLIGRPFEEETLFSLAQVVEE
AAGRFPVDDLWWK
>Mature_492_residues
TALNQLTLTEAREGLATGEFTAVELTQSYLDAMAEARTLNAYVLETADKALAMAAESDKRIASGDAGKLEGLPIGVKDMF
CTDGVRTTAGSKILGDFVPPYESTVTSQLWRDGAVMLGKLNNDEFAMGSSNETSAQGPVVSPWRREGDSAPLVPGGSSGG
SAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIIAYASSLDQAGPFARTVNDAAVLLRSMAGYDPKD
STSVNRSVPDYEDAVGASIKGKRIGIPREYRVEGLDPEIAGMWEEGAQWLRDAGAEVVDISLPHTKYALPTYYIVAPAEA
SSNLARYDGVRYGTRVPGRDIIDMYERTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYVKAQKVRTLIKRDFDDVFAQGVD
AVLTPATPSPAFGIGEKGSADPVEMYLQDIFTVTVNLAGLPGISVPAGLSCKNLPLGLQLIGRPFEEETLFSLAQVVEEA
AGRFPVDDLWWK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=43.0830039525692, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=24.9488752556237, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=36.4835164835165, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=25.3493013972056, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=237, Percent_Identity=29.957805907173, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=523, Percent_Identity=22.5621414913958, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=35.2668213457077, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=458, Percent_Identity=27.5109170305677, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648113, Length=485, Percent_Identity=38.9690721649485, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=25.5060728744939, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=25.5060728744939, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=25.5060728744939, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=25.5060728744939, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=25.6972111553785, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=24.4047619047619, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_AZOC5 (A8I9X6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525760.1
- ProteinModelPortal:   A8I9X6
- SMR:   A8I9X6
- GeneID:   5689398
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2844
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   ACAU438753:AZC_2844-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52504; Mature: 52372

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNQLTLTEAREGLATGEFTAVELTQSYLDAMAEARTLNAYVLETADKALAMAAESDK
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCC
RIASGDAGKLEGLPIGVKDMFCTDGVRTTAGSKILGDFVPPYESTVTSQLWRDGAVMLGK
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSAQGPVVSPWRREGDSAPLVPGGSSGGSAAAVAAFLCAGATGTDTG
CCCCCEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRFGIIAYASSLDQAGPFARTVNDAAVLLRSMAGYDPK
CCCCCCCCEECCCCCCCCCCCHHHCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
DSTSVNRSVPDYEDAVGASIKGKRIGIPREYRVEGLDPEIAGMWEEGAQWLRDAGAEVVD
CCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
ISLPHTKYALPTYYIVAPAEASSNLARYDGVRYGTRVPGRDIIDMYERTRAAGFGPEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
RIMIGTYVLSAGYYDAYYVKAQKVRTLIKRDFDDVFAQGVDAVLTPATPSPAFGIGEKGS
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCHHHHHHCCCCEEECCCCCCCCCCCCCCCC
ADPVEMYLQDIFTVTVNLAGLPGISVPAGLSCKNLPLGLQLIGRPFEEETLFSLAQVVEE
CCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
AAGRFPVDDLWWK
HHCCCCCHHHCCC
>Mature Secondary Structure 
TALNQLTLTEAREGLATGEFTAVELTQSYLDAMAEARTLNAYVLETADKALAMAAESDK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCC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HHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA