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The map label for this gene is glpF [H]

Identifier: 158424335

GI number: 158424335

Start: 3077733

End: 3078458

Strand: Direct

Name: glpF [H]

Synonym: AZC_2711

Alternate gene names: 158424335

Gene position: 3077733-3078458 (Clockwise)

Preceding gene: 158424334

Following gene: 158424336

Centisome position: 57.32

GC content: 67.63

Gene sequence:

>726_bases
ATGCACACCCCCTTTACCGGCGAGCTGCTCGGCACGCTCGTTCTCATCCTGCTCGGCGATGGCGTCGTGGCGGGCGTATT
GCTCAACCACTCCAAGGCCCAGAATTCGGGCTGGATCGTCATCACCGCCGGCTGGGCCTTCGCAGTGATGGCCGGCGTTT
TCACCTCGGCCGCCACCGGCAGCCCGGATGCGCACCTCAACCCCGCCGTGACGCTCGGCTTTGCCATCGTGTCCGGCGAT
TATTCGAAGCTCGCGGTCTATATCCCCGCCCAGCTCATCGGCGCCTTCCTCGGGGCCACGCTGGTGTGGCTCCAGTATCT
GCCGCACTGGTCGAAGACACCCGATCAGGGCCTGAAGCTCGCCATTTTCTGCACCGGCCCGGCCATCCGCAACGCGCCTG
CCAACCTCATCAGCGAGATCATCGGCACGGTGGTCCTGCTGTTCGTCATCGCCGCCATCTTCTCGAAGCCGGTGATGGGC
GCGGGCATTCCCTCGACGCTTGGCCCCTACATGGTCGGCATCCTCGTGTGGGCCATCGGCCTCTCGCTCGGCGGCACCAC
CGGCTACGCCATCAACCCGGCCCGCGACCTCGGCCCGCGCATCGCCCATGCGGTGCTCCCTGTGGCCGGCAAGGGCGGCT
CGGACTGGGGCTACTCCTGGATCCCCGTGGTCGGCCCGCTCGCCGGCGCCGTGGTGGCCGGCCTCCTCATCAAGGCGTTC
ATCTGA

Upstream 100 bases:

>100_bases
CTCTTGATGGCAGAGACCGCATGGCCGATGGGTCCGCGACGCGCGCGCATCGGCTCATGCTCTTGAACAATCAATCAAGA
ATGGGTTCTGGAGACGTCCC

Downstream 100 bases:

>100_bases
CGCCATCCGCCGCGTTTTGAAGTTACAGCAGCCCCCGGCCCAAAAGCCGGGGGTTTTCTGTTTCGGCCTCCACCTCACCT
TGCGGCAGGGCACCGAGGAC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MHTPFTGELLGTLVLILLGDGVVAGVLLNHSKAQNSGWIVITAGWAFAVMAGVFTSAATGSPDAHLNPAVTLGFAIVSGD
YSKLAVYIPAQLIGAFLGATLVWLQYLPHWSKTPDQGLKLAIFCTGPAIRNAPANLISEIIGTVVLLFVIAAIFSKPVMG
AGIPSTLGPYMVGILVWAIGLSLGGTTGYAINPARDLGPRIAHAVLPVAGKGGSDWGYSWIPVVGPLAGAVVAGLLIKAF
I

Sequences:

>Translated_241_residues
MHTPFTGELLGTLVLILLGDGVVAGVLLNHSKAQNSGWIVITAGWAFAVMAGVFTSAATGSPDAHLNPAVTLGFAIVSGD
YSKLAVYIPAQLIGAFLGATLVWLQYLPHWSKTPDQGLKLAIFCTGPAIRNAPANLISEIIGTVVLLFVIAAIFSKPVMG
AGIPSTLGPYMVGILVWAIGLSLGGTTGYAINPARDLGPRIAHAVLPVAGKGGSDWGYSWIPVVGPLAGAVVAGLLIKAF
I
>Mature_241_residues
MHTPFTGELLGTLVLILLGDGVVAGVLLNHSKAQNSGWIVITAGWAFAVMAGVFTSAATGSPDAHLNPAVTLGFAIVSGD
YSKLAVYIPAQLIGAFLGATLVWLQYLPHWSKTPDQGLKLAIFCTGPAIRNAPANLISEIIGTVVLLFVIAAIFSKPVMG
AGIPSTLGPYMVGILVWAIGLSLGGTTGYAINPARDLGPRIAHAVLPVAGKGGSDWGYSWIPVVGPLAGAVVAGLLIKAF
I

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=242, Percent_Identity=37.603305785124, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI22538420, Length=237, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI4502187, Length=263, Percent_Identity=32.319391634981, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1790362, Length=253, Percent_Identity=32.8063241106719, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994009, Length=240, Percent_Identity=31.6666666666667, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=252, Percent_Identity=34.5238095238095, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=235, Percent_Identity=32.7659574468085, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531429, Length=235, Percent_Identity=32.7659574468085, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=31.6666666666667, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=31.6666666666667, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=30.6201550387597, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17558372, Length=247, Percent_Identity=27.9352226720648, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=33.175355450237, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322985, Length=288, Percent_Identity=28.8194444444444, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652747, Length=237, Percent_Identity=31.6455696202532, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45551084, Length=237, Percent_Identity=31.6455696202532, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24703; Mature: 24703

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPFTGELLGTLVLILLGDGVVAGVLLNHSKAQNSGWIVITAGWAFAVMAGVFTSAATG
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCC
SPDAHLNPAVTLGFAIVSGDYSKLAVYIPAQLIGAFLGATLVWLQYLPHWSKTPDQGLKL
CCCCCCCHHHHHHHHEEECCCHHEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AIFCTGPAIRNAPANLISEIIGTVVLLFVIAAIFSKPVMGAGIPSTLGPYMVGILVWAIG
EEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGGTTGYAINPARDLGPRIAHAVLPVAGKGGSDWGYSWIPVVGPLAGAVVAGLLIKAF
HHCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
I
C
>Mature Secondary Structure
MHTPFTGELLGTLVLILLGDGVVAGVLLNHSKAQNSGWIVITAGWAFAVMAGVFTSAATG
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCC
SPDAHLNPAVTLGFAIVSGDYSKLAVYIPAQLIGAFLGATLVWLQYLPHWSKTPDQGLKL
CCCCCCCHHHHHHHHEEECCCHHEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AIFCTGPAIRNAPANLISEIIGTVVLLFVIAAIFSKPVMGAGIPSTLGPYMVGILVWAIG
EEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGGTTGYAINPARDLGPRIAHAVLPVAGKGGSDWGYSWIPVVGPLAGAVVAGLLIKAF
HHCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]