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The map label for this gene is glpK

Identifier: 158424334

GI number: 158424334

Start: 3076038

End: 3077534

Strand: Direct

Name: glpK

Synonym: AZC_2710

Alternate gene names: 158424334

Gene position: 3076038-3077534 (Clockwise)

Preceding gene: 158424333

Following gene: 158424335

Centisome position: 57.28

GC content: 66.53

Gene sequence:

>1497_bases
ATGACCAAATACGTGGGCGCCATCGATCAGGGCACGACGAGCACGCGCTTCATCGTGTTCGACCGCAAGGGCCAGATCTT
CAGCGTGGCCCAGCGCGAGCATGAGCAGATCTATCCCCGGCCGGGCTGGGTGGAGCACAATGCGGTCGAGATCTGGCTCA
ACACCCGCACGGTGATCCTGGAGGCCCTCGAGAAGAAGGGTCTCTCCACGTCCGACCTCGCCGCCGTCGGCGTGACCAAC
CAGCGCGAAACCGCGCTCCTGTGGGACCGCAAAACCGGCGAACCGCTCTACAACGCCCTCGTCTGGCAGGACACCCGCAC
CGACCAGCTCGTCGCCCGCTACGCGAAGGAGGGTGGCCAGGACCGACTGCGTGCCAAGACCGGCCTGCCGCTCGCCACCT
ATTTCTCCGGCCTGAAGCTGCACTGGATCCTCGACAACGTGCCCGGCGCCCGCGCCAAGGCCGAGGCCGGCGACGCGCTG
TTCGGCAATATCGATACCTGGCTGCTCTGGAACCTCACCGGCGGTCCGGACGGCGGCATCCACATCACCGACGTCACCAA
CGCCTCGCGCACCCAGCTCATGGACCTCGAGAAGCTGGCGTGGGACGAGGACATCCTGAAGCTGTTCAACATCCCGGCTG
CCTGCCTGCCGCGCATCGTCTCCTCCTCGGAGCGCTACGGCACCGGCAAGGGGCCGCTGGAAGGCGTGACGCTCTCCGGC
ATCCTCGGCGACCAGCAGGCGGCCCTGTTCGGCCAGGCCTGCCTGCATCCGGGCGAAGCCAAGAACACCTACGGCACCGG
CAACTTCATGCTGATGAACACCGGTGAGAAGCCCTTCCCCTCCACCTGCGGTCTCATCACGACGGTGGGCTACAAGCTGG
GCGATGCGAAAGCGGTCTATGCGCTGGAAGGTTCCATCGCCATCACCGGCGCTCTGGTGCAGTGGCTGCGCGACAACCTC
GGCATCATCAAGAACTCGGCCGACATCGAGCCGCTCGCCCGCACGGTCAGCGATAACGGCGACGTCTATTTCGTCCCCGC
CTTCTCCGGCCTTTATGCGCCGCGGTGGGATGACAGCGCGCGCGGCGTCGTCTGCGGTCTCACCCGCTTCGCCAACAAGG
GCCACATCGCCCGCGCCGCGCTGGAGGCGACCGCCTACCAGACGCGCGAGGTGCTGGAAGCCATGGTGAAGGATTCCAAG
GTCGCCATTCGCGAGCTGCGCACCGACGGCGGCATGGTGGTCAATGAACTGCTCATGCAGTTCCAGGCCGATATGGTGAA
CGTGCCGGTCGTCCGCCCCAAGGTCATCGAGACCACGGCGCTGGGCGCCGCTTATGCGGCGGGCCTTGCCGTCGGCTACT
GGGCGTCCACCGACGACATCACCCAGAACTGGGGTGTCGACCGGCGCTGGCACCCGCACATGGCTGCGGAGCAGCGCGAA
CACCTGTATGGCTCGTGGAACAAGGCCGTTGAGCGCTCCCTCGGTTGGGCGCGCTGA

Upstream 100 bases:

>100_bases
AGAGGCGGCTGCCGAAATCGACGCATTCCTCAACAGCATCTGACGCCGGCCGCACCCAAGACGCGGACGGATGCCGGCAA
GGTTAAAACGGGAGGAGACC

Downstream 100 bases:

>100_bases
CGGTGTAAGGTGTCCCGTTCCGGCGGCTGGCAAAGCAGCCTCCGGCACGGTCTGAGGCGTGATCCGAAGGGAGTATTCAC
CTCGTCGGCAGTCGCGCCCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTKYVGAIDQGTTSTRFIVFDRKGQIFSVAQREHEQIYPRPGWVEHNAVEIWLNTRTVILEALEKKGLSTSDLAAVGVTN
QRETALLWDRKTGEPLYNALVWQDTRTDQLVARYAKEGGQDRLRAKTGLPLATYFSGLKLHWILDNVPGARAKAEAGDAL
FGNIDTWLLWNLTGGPDGGIHITDVTNASRTQLMDLEKLAWDEDILKLFNIPAACLPRIVSSSERYGTGKGPLEGVTLSG
ILGDQQAALFGQACLHPGEAKNTYGTGNFMLMNTGEKPFPSTCGLITTVGYKLGDAKAVYALEGSIAITGALVQWLRDNL
GIIKNSADIEPLARTVSDNGDVYFVPAFSGLYAPRWDDSARGVVCGLTRFANKGHIARAALEATAYQTREVLEAMVKDSK
VAIRELRTDGGMVVNELLMQFQADMVNVPVVRPKVIETTALGAAYAAGLAVGYWASTDDITQNWGVDRRWHPHMAAEQRE
HLYGSWNKAVERSLGWAR

Sequences:

>Translated_498_residues
MTKYVGAIDQGTTSTRFIVFDRKGQIFSVAQREHEQIYPRPGWVEHNAVEIWLNTRTVILEALEKKGLSTSDLAAVGVTN
QRETALLWDRKTGEPLYNALVWQDTRTDQLVARYAKEGGQDRLRAKTGLPLATYFSGLKLHWILDNVPGARAKAEAGDAL
FGNIDTWLLWNLTGGPDGGIHITDVTNASRTQLMDLEKLAWDEDILKLFNIPAACLPRIVSSSERYGTGKGPLEGVTLSG
ILGDQQAALFGQACLHPGEAKNTYGTGNFMLMNTGEKPFPSTCGLITTVGYKLGDAKAVYALEGSIAITGALVQWLRDNL
GIIKNSADIEPLARTVSDNGDVYFVPAFSGLYAPRWDDSARGVVCGLTRFANKGHIARAALEATAYQTREVLEAMVKDSK
VAIRELRTDGGMVVNELLMQFQADMVNVPVVRPKVIETTALGAAYAAGLAVGYWASTDDITQNWGVDRRWHPHMAAEQRE
HLYGSWNKAVERSLGWAR
>Mature_497_residues
TKYVGAIDQGTTSTRFIVFDRKGQIFSVAQREHEQIYPRPGWVEHNAVEIWLNTRTVILEALEKKGLSTSDLAAVGVTNQ
RETALLWDRKTGEPLYNALVWQDTRTDQLVARYAKEGGQDRLRAKTGLPLATYFSGLKLHWILDNVPGARAKAEAGDALF
GNIDTWLLWNLTGGPDGGIHITDVTNASRTQLMDLEKLAWDEDILKLFNIPAACLPRIVSSSERYGTGKGPLEGVTLSGI
LGDQQAALFGQACLHPGEAKNTYGTGNFMLMNTGEKPFPSTCGLITTVGYKLGDAKAVYALEGSIAITGALVQWLRDNLG
IIKNSADIEPLARTVSDNGDVYFVPAFSGLYAPRWDDSARGVVCGLTRFANKGHIARAALEATAYQTREVLEAMVKDSKV
AIRELRTDGGMVVNELLMQFQADMVNVPVVRPKVIETTALGAAYAAGLAVGYWASTDDITQNWGVDRRWHPHMAAEQREH
LYGSWNKAVERSLGWAR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=52.3809523809524, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=52.3809523809524, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=51.7647058823529, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI41393575, Length=505, Percent_Identity=52.0792079207921, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=32.2896281800391, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=50.1002004008016, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1790005, Length=475, Percent_Identity=24.2105263157895, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789168, Length=480, Percent_Identity=24.7916666666667, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=45.4545454545455, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71986140, Length=528, Percent_Identity=26.7045454545455, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71986147, Length=306, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321755, Length=553, Percent_Identity=37.7938517179023, Blast_Score=329, Evalue=6e-91,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=48.5148514851485, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24655555, Length=507, Percent_Identity=44.9704142011834, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655560, Length=507, Percent_Identity=44.9704142011834, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655570, Length=507, Percent_Identity=44.9704142011834, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655574, Length=507, Percent_Identity=44.9704142011834, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655565, Length=507, Percent_Identity=44.9704142011834, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24654573, Length=437, Percent_Identity=49.8855835240275, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=34.6516007532957, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=35.6415478615071, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24656367, Length=511, Percent_Identity=29.3542074363992, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=28.7619047619048, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=30.3724928366762, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=28.2758620689655, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_AZOC5 (A8I8V7)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525626.1
- ProteinModelPortal:   A8I8V7
- SMR:   A8I8V7
- GeneID:   5691223
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2710
- HOGENOM:   HBG511469
- OMA:   KILPNVR
- ProtClustDB:   CLSK978155
- BioCyc:   ACAU438753:AZC_2710-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54640; Mature: 54508

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVGAIDQGTTSTRFIVFDRKGQIFSVAQREHEQIYPRPGWVEHNAVEIWLNTRTVIL
CCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
EALEKKGLSTSDLAAVGVTNQRETALLWDRKTGEPLYNALVWQDTRTDQLVARYAKEGGQ
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHEEECCCCHHHHHHHHHHCCCH
DRLRAKTGLPLATYFSGLKLHWILDNVPGARAKAEAGDALFGNIDTWLLWNLTGGPDGGI
HHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCE
HITDVTNASRTQLMDLEKLAWDEDILKLFNIPAACLPRIVSSSERYGTGKGPLEGVTLSG
EEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEE
ILGDQQAALFGQACLHPGEAKNTYGTGNFMLMNTGEKPFPSTCGLITTVGYKLGDAKAVY
ECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCEECCCCEEEE
ALEGSIAITGALVQWLRDNLGIIKNSADIEPLARTVSDNGDVYFVPAFSGLYAPRWDDSA
EECCCEEHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
RGVVCGLTRFANKGHIARAALEATAYQTREVLEAMVKDSKVAIRELRTDGGMVVNELLMQ
CCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
FQADMVNVPVVRPKVIETTALGAAYAAGLAVGYWASTDDITQNWGVDRRWHPHMAAEQRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCCCCCCHHHHHHH
HLYGSWNKAVERSLGWAR
HHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TKYVGAIDQGTTSTRFIVFDRKGQIFSVAQREHEQIYPRPGWVEHNAVEIWLNTRTVIL
CCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
EALEKKGLSTSDLAAVGVTNQRETALLWDRKTGEPLYNALVWQDTRTDQLVARYAKEGGQ
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHEEECCCCHHHHHHHHHHCCCH
DRLRAKTGLPLATYFSGLKLHWILDNVPGARAKAEAGDALFGNIDTWLLWNLTGGPDGGI
HHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCE
HITDVTNASRTQLMDLEKLAWDEDILKLFNIPAACLPRIVSSSERYGTGKGPLEGVTLSG
EEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEE
ILGDQQAALFGQACLHPGEAKNTYGTGNFMLMNTGEKPFPSTCGLITTVGYKLGDAKAVY
ECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCEECCCCEEEE
ALEGSIAITGALVQWLRDNLGIIKNSADIEPLARTVSDNGDVYFVPAFSGLYAPRWDDSA
EECCCEEHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
RGVVCGLTRFANKGHIARAALEATAYQTREVLEAMVKDSKVAIRELRTDGGMVVNELLMQ
CCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
FQADMVNVPVVRPKVIETTALGAAYAAGLAVGYWASTDDITQNWGVDRRWHPHMAAEQRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCCCCCCHHHHHHH
HLYGSWNKAVERSLGWAR
HHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA