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The map label for this gene is appA [H]

Identifier: 158424283

GI number: 158424283

Start: 3012633

End: 3014249

Strand: Direct

Name: appA [H]

Synonym: AZC_2659

Alternate gene names: 158424283

Gene position: 3012633-3014249 (Clockwise)

Preceding gene: 158424282

Following gene: 158424284

Centisome position: 56.1

GC content: 63.76

Gene sequence:

>1617_bases
ATGTCGGGTCCCTTTGTCGACCGCCGTTCTTTCTCCCTCGGCCTTGCCGCTTCCCTTGGCCTCGCACCCCTCGCTGGCCG
TGCCGCGTTCGCGGCCGGCGCGGATGCAGGCGGCACGCCGGTGCGCGGCGGGGTGCTGCGTTTTCTCGTGGAGCAGGAAC
CGACGACGCTCGTCACCATCGCCCACACCGCCGGCCCCTCCACCCGCGTCTCGCCCAAGGTGACGGAGGGCCTGCTGCAA
TATGGCTTCGACTATGAGCCGAAACCCCAGCTCGCAACCGAGTGGAGCGAGAGCCCGGACGGCCTCACCTATGTCTTCAA
GCTGCGTCCCAACGTGAAATGGCATGACGGCAAGGATTTCACCTCCGCCGACGTCGCCTATTCCATCAAGCTGCTCAAGG
AAAACCACCCGCGCGGGCGCGCCACCTTCTCGTCTGTCACCGTGGTGGAGACGCCCGATCCGCTCACCGCCATCATCCGC
CTGTCGAAGCCGGCGCCCTATCTCTTGGTGGCGCTCGATGCCTCCGAATCCCCCATCGTGCCCAAGCACCTCTATGAGGG
CACCGATCCGCTGACCAACAAGAACGCCAGCGCCCCCGTGGGCACCGGGCCGTTCGTGTTCCGTGAATGGGTGAAGGGCA
GCCACATCATCCTCGACCGCAACCCCAATTATTGGGACCCCGGCAAGCCTTATCTCGACCGCGTCGTGGTCCGCTTCATC
GCCGACCAGAGCGCCCGTTCGGCCGCCTTCGAGACCGGCGAACTGGATCTCGGCGGCGGGCCGCCGGTGCCGCGTTCCGA
TCTCGCCCGCATTCAGGCCCTGCCGAACATCGGCTCGGACACCCGGGGCTATGAATACAACGGCAACATGACCCAGCTCT
TCTTCAACTTCGATACGCCGGTGCTGAAGGACAAGAAGGTGCGCCTCGCCATCGCCAAGGCCATCGACCACAAGAAACTG
CTGGACGTCGTGTATTTCGGCTACGGCACCATCGCGCCCTCGCCGCTCGGCGTCGATGTGAAGAAGTTCATCGACCCGAA
CATCAAGACCTATGGCTTCGATCTGTCTGCCGCCAACAAGCTGCTGGACGAGGCCGGCTATCCGCGCAAGGCCGATGGCA
CGCGCTTTCCGCTGCGCCTCTATGCCAATCCGTACAACTCCCAGGCCTCCGGGGATTTCGTGAAGCAGGCGCTGGCGCGC
ATCGGCATTCCGGTGGATTTCCAGTTCTTCGATTTCTCCACCTATCTCCAGAAGGCCTACACGGTCCGCGACTTCGACCT
GACGCTGGAATCCCTCTCCACCACGTTCGACCCGACGCCCGGCGTGCAGCGCGTCTACTGGTCCAAGAACTTCAAGATCG
GCCTGCCCTTCTCCAACGCCTCCCATTACGACAATCCGGAGGTGGATGCCTTGCTGGAAGCCGCCGCTGTGGAGGCGGAC
GAGGAGAAGCGCATCGCCATCTGGCGCAAGTTCCAGCAGGTGACCCATGACGACGTGGCGGCCGTGAACCTCATCTCGCC
GGCGGGCGTCACCCTCTTCAACAAGAAGGTGAAGAACCACACGCTGGGCGTGACGGGCGTGAACGCCAGCTTCGCCGACG
TTTATTTCGAGAAGTGA

Upstream 100 bases:

>100_bases
GTGCTCACCGGCGAACCGCCGGCCCCCAGCACGTTCCAATAGGCCGCAGTTTCGGCCCCGCTCCGCTCTCCCCCAGCCTT
ATCCGAGGACATTCACCGCC

Downstream 100 bases:

>100_bases
TCCGGCTTTCAGGAGACACATCCATGCCCGGACCGCTGCGCGTCGCAAAGGCGCTCCGTCGCACCGCCCTCCAGGCCGTG
CCGACGGTCCTCATCATCGT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSGPFVDRRSFSLGLAASLGLAPLAGRAAFAAGADAGGTPVRGGVLRFLVEQEPTTLVTIAHTAGPSTRVSPKVTEGLLQ
YGFDYEPKPQLATEWSESPDGLTYVFKLRPNVKWHDGKDFTSADVAYSIKLLKENHPRGRATFSSVTVVETPDPLTAIIR
LSKPAPYLLVALDASESPIVPKHLYEGTDPLTNKNASAPVGTGPFVFREWVKGSHIILDRNPNYWDPGKPYLDRVVVRFI
ADQSARSAAFETGELDLGGGPPVPRSDLARIQALPNIGSDTRGYEYNGNMTQLFFNFDTPVLKDKKVRLAIAKAIDHKKL
LDVVYFGYGTIAPSPLGVDVKKFIDPNIKTYGFDLSAANKLLDEAGYPRKADGTRFPLRLYANPYNSQASGDFVKQALAR
IGIPVDFQFFDFSTYLQKAYTVRDFDLTLESLSTTFDPTPGVQRVYWSKNFKIGLPFSNASHYDNPEVDALLEAAAVEAD
EEKRIAIWRKFQQVTHDDVAAVNLISPAGVTLFNKKVKNHTLGVTGVNASFADVYFEK

Sequences:

>Translated_538_residues
MSGPFVDRRSFSLGLAASLGLAPLAGRAAFAAGADAGGTPVRGGVLRFLVEQEPTTLVTIAHTAGPSTRVSPKVTEGLLQ
YGFDYEPKPQLATEWSESPDGLTYVFKLRPNVKWHDGKDFTSADVAYSIKLLKENHPRGRATFSSVTVVETPDPLTAIIR
LSKPAPYLLVALDASESPIVPKHLYEGTDPLTNKNASAPVGTGPFVFREWVKGSHIILDRNPNYWDPGKPYLDRVVVRFI
ADQSARSAAFETGELDLGGGPPVPRSDLARIQALPNIGSDTRGYEYNGNMTQLFFNFDTPVLKDKKVRLAIAKAIDHKKL
LDVVYFGYGTIAPSPLGVDVKKFIDPNIKTYGFDLSAANKLLDEAGYPRKADGTRFPLRLYANPYNSQASGDFVKQALAR
IGIPVDFQFFDFSTYLQKAYTVRDFDLTLESLSTTFDPTPGVQRVYWSKNFKIGLPFSNASHYDNPEVDALLEAAAVEAD
EEKRIAIWRKFQQVTHDDVAAVNLISPAGVTLFNKKVKNHTLGVTGVNASFADVYFEK
>Mature_537_residues
SGPFVDRRSFSLGLAASLGLAPLAGRAAFAAGADAGGTPVRGGVLRFLVEQEPTTLVTIAHTAGPSTRVSPKVTEGLLQY
GFDYEPKPQLATEWSESPDGLTYVFKLRPNVKWHDGKDFTSADVAYSIKLLKENHPRGRATFSSVTVVETPDPLTAIIRL
SKPAPYLLVALDASESPIVPKHLYEGTDPLTNKNASAPVGTGPFVFREWVKGSHIILDRNPNYWDPGKPYLDRVVVRFIA
DQSARSAAFETGELDLGGGPPVPRSDLARIQALPNIGSDTRGYEYNGNMTQLFFNFDTPVLKDKKVRLAIAKAIDHKKLL
DVVYFGYGTIAPSPLGVDVKKFIDPNIKTYGFDLSAANKLLDEAGYPRKADGTRFPLRLYANPYNSQASGDFVKQALARI
GIPVDFQFFDFSTYLQKAYTVRDFDLTLESLSTTFDPTPGVQRVYWSKNFKIGLPFSNASHYDNPEVDALLEAAAVEADE
EKRIAIWRKFQQVTHDDVAAVNLISPAGVTLFNKKVKNHTLGVTGVNASFADVYFEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=495, Percent_Identity=27.0707070707071, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787052, Length=482, Percent_Identity=27.3858921161826, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789966, Length=477, Percent_Identity=25.5765199161426, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=23.8532110091743, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789887, Length=448, Percent_Identity=23.8839285714286, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787551, Length=484, Percent_Identity=23.5537190082645, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789397, Length=506, Percent_Identity=23.9130434782609, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87081878, Length=497, Percent_Identity=24.748490945674, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87082063, Length=447, Percent_Identity=24.8322147651007, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59033; Mature: 58902

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPFVDRRSFSLGLAASLGLAPLAGRAAFAAGADAGGTPVRGGVLRFLVEQEPTTLVTI
CCCCCCCCCCCCCCHHHHCCCCCCCCCHHEECCCCCCCCCCCCHHHEEEECCCCCEEEEE
AHTAGPSTRVSPKVTEGLLQYGFDYEPKPQLATEWSESPDGLTYVFKLRPNVKWHDGKDF
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCEECCCCCC
TSADVAYSIKLLKENHPRGRATFSSVTVVETPDPLTAIIRLSKPAPYLLVALDASESPIV
CCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCC
PKHLYEGTDPLTNKNASAPVGTGPFVFREWVKGSHIILDRNPNYWDPGKPYLDRVVVRFI
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
ADQSARSAAFETGELDLGGGPPVPRSDLARIQALPNIGSDTRGYEYNGNMTQLFFNFDTP
HCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCC
VLKDKKVRLAIAKAIDHKKLLDVVYFGYGTIAPSPLGVDVKKFIDPNIKTYGFDLSAANK
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEECCHHHHHH
LLDEAGYPRKADGTRFPLRLYANPYNSQASGDFVKQALARIGIPVDFQFFDFSTYLQKAY
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
TVRDFDLTLESLSTTFDPTPGVQRVYWSKNFKIGLPFSNASHYDNPEVDALLEAAAVEAD
EEEEHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCC
EEKRIAIWRKFQQVTHDDVAAVNLISPAGVTLFNKKVKNHTLGVTGVNASFADVYFEK
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHCCCEEEEEECCCCHHEEEECC
>Mature Secondary Structure 
SGPFVDRRSFSLGLAASLGLAPLAGRAAFAAGADAGGTPVRGGVLRFLVEQEPTTLVTI
CCCCCCCCCCCCCHHHHCCCCCCCCCHHEECCCCCCCCCCCCHHHEEEECCCCCEEEEE
AHTAGPSTRVSPKVTEGLLQYGFDYEPKPQLATEWSESPDGLTYVFKLRPNVKWHDGKDF
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCEECCCCCC
TSADVAYSIKLLKENHPRGRATFSSVTVVETPDPLTAIIRLSKPAPYLLVALDASESPIV
CCCCCEEEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCC
PKHLYEGTDPLTNKNASAPVGTGPFVFREWVKGSHIILDRNPNYWDPGKPYLDRVVVRFI
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
ADQSARSAAFETGELDLGGGPPVPRSDLARIQALPNIGSDTRGYEYNGNMTQLFFNFDTP
HCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCC
VLKDKKVRLAIAKAIDHKKLLDVVYFGYGTIAPSPLGVDVKKFIDPNIKTYGFDLSAANK
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEECCHHHHHH
LLDEAGYPRKADGTRFPLRLYANPYNSQASGDFVKQALARIGIPVDFQFFDFSTYLQKAY
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
TVRDFDLTLESLSTTFDPTPGVQRVYWSKNFKIGLPFSNASHYDNPEVDALLEAAAVEAD
EEEEHHHHHHHHHCCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCC
EEKRIAIWRKFQQVTHDDVAAVNLISPAGVTLFNKKVKNHTLGVTGVNASFADVYFEK
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHCCCEEEEEECCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]