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The map label for this gene is gsiB [H]

Identifier: 158424279

GI number: 158424279

Start: 3007635

End: 3009269

Strand: Direct

Name: gsiB [H]

Synonym: AZC_2655

Alternate gene names: 158424279

Gene position: 3007635-3009269 (Clockwise)

Preceding gene: 158424278

Following gene: 158424280

Centisome position: 56.01

GC content: 65.69

Gene sequence:

>1635_bases
ATGACCTCAGAGCTTGATCGCCTGCCCTTCGGTCCCATCTCCCGCCGCCTGTTCCTTGGCGGCGCGGGCGCTGCCGCCGC
AACTTTGTTCATCGGCCAGAACGGCAAGGCCTTTGCCGCCGAGACCGGGCAGAAGGGCGGCACGCTCACCATGGCCGTGT
GGCCGGACCCGACCGCGCTGGTGTGCGCCTTCACGTCTTCCGATCAGGTGGTGCTCTCCTCCGCCAAGATGACCGAGGGG
CTCGTGGCCTATGGCTACGACTTCCTGCCCCGCCCGCGCCTTGCCACCGCGTGGGAGCCGTCCGCCGACGGTCTGTCCAT
CAAGTTCACGCTCCGCCAGGGCGTGAAGTGGCATGACGGGACGGACTTCACCTCCGCCGACGTCGCCTTTTCCTTCCTCA
ACCTGCTGAAGCCGAACCATCCGCGCGGGCGTGGCACCTTCGCCAACCTCACCGCCGTGGACACGCCGGACGCCCACACC
GCCATCCTGCGCTTCTCCAAGCCCTCGCCGGTGGTCATGAACGCGCTGTCGGGCATGGAAAGCCCCATCCTGCCCAAGCA
CATCTATGAGAATGGGGACCCGCTGCGGAACCCGGCCAACAACGCGCCCATCGGCACCGGCCCGTTCAAATTCGTGGAAT
GGAAGCGCGGCAGCCACATCATCCTGGAGCGCTTCGACGGCTATTGGGCGCAGGAGCGGCCCTATCTCGACCGCATCGTG
ATGCGCATCATCAACGATGCCGGTGCCCGCGCAGCAGCGCTGGAGACGGGAGAGATCCTGCTCGCCGGCCCCAATCCCGT
GCCCTACAGCGAGTTGGCGCGCTTCCGTGCCAATCCGAAGTTCGACGTCGAGACACGCGGTGAGGAGCTTCTCCAGCACG
CGCAGGCCATCGAGGTGAACCTGCGCAACCCCATCCTCGCCAAGGTGGAGGTGCGCCAGGCCATCCTTCAGGCGGTCAAC
CGCGAGGCCATGGCCCGCGCGGTCTGGTATCAGAGCGGCCGTCCCGCCACGAGCCCGATCCCCTATCAGGCCGGCGACCT
CAGGCTCGCCAATCCGCCCGCCTATCCCTTCGACGTCAAGAAGGCAGAAGCCCTGCTCGATGCCGCCGGCTATCCGCGCG
GCGCGGACAAGATGCGCTTCAAACTGCGCCTCGACTGGCTGCCGCTCGGCGAAGCCAACCTGCGGGCGGCGGAATTCATC
AAGCAGTCGCTCCAGCGGGTGGGCATCGACATCACCATCCGCTCCTCCGACCTGCCGACTTACCTCAAGACGATCTATGG
CGACTATGATTACGACCTGAACGTTTTCCTCTACGCACCCATCTTCGACCCCTCCATGGGCCTCCAGCGCTTCTACTGGT
CGAAGGCGGCCAAGCCGGGCTCGCCCTTCGTGATCGCCAGCGGCTATTCCAGCCCGGAGATGGACAAGATTCTCGAAAGC
GCCTCCACGGAGATGGATCCGGCCAAGCGCAAGGCGCTGTTCCACGACTTCCAGCGCCTCGCCATGACCGACCTCCCCGT
GCTGCCGCTGCTCGATCTCGATTACACCGCGATCATCAGCAAGCGCGTGCATGGCGTCATGGACATGCCGGAGGGCATCC
GCGGCAGCTTCGAGAACGTCTGGCTCTCAGCCTGA

Upstream 100 bases:

>100_bases
CCGCGTAGAGGTGACCGGTGCAGGCGAGGCCCGTTCCCCGTCCGAGATTCTCTCCCTTGAACCCATCACGGCGCCTGCGC
CCGTCTGAACGAGACCCGTC

Downstream 100 bases:

>100_bases
GGACGTTTCCCGGACGGGATGCGCCAGCGCGTATCCCGTCTGGCTGCCTAAGAGCCCGTTTGGAAATTCGTGATCCGGGG
ATTCCTGTTTGTCCGTGCTT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTSELDRLPFGPISRRLFLGGAGAAAATLFIGQNGKAFAAETGQKGGTLTMAVWPDPTALVCAFTSSDQVVLSSAKMTEG
LVAYGYDFLPRPRLATAWEPSADGLSIKFTLRQGVKWHDGTDFTSADVAFSFLNLLKPNHPRGRGTFANLTAVDTPDAHT
AILRFSKPSPVVMNALSGMESPILPKHIYENGDPLRNPANNAPIGTGPFKFVEWKRGSHIILERFDGYWAQERPYLDRIV
MRIINDAGARAAALETGEILLAGPNPVPYSELARFRANPKFDVETRGEELLQHAQAIEVNLRNPILAKVEVRQAILQAVN
REAMARAVWYQSGRPATSPIPYQAGDLRLANPPAYPFDVKKAEALLDAAGYPRGADKMRFKLRLDWLPLGEANLRAAEFI
KQSLQRVGIDITIRSSDLPTYLKTIYGDYDYDLNVFLYAPIFDPSMGLQRFYWSKAAKPGSPFVIASGYSSPEMDKILES
ASTEMDPAKRKALFHDFQRLAMTDLPVLPLLDLDYTAIISKRVHGVMDMPEGIRGSFENVWLSA

Sequences:

>Translated_544_residues
MTSELDRLPFGPISRRLFLGGAGAAAATLFIGQNGKAFAAETGQKGGTLTMAVWPDPTALVCAFTSSDQVVLSSAKMTEG
LVAYGYDFLPRPRLATAWEPSADGLSIKFTLRQGVKWHDGTDFTSADVAFSFLNLLKPNHPRGRGTFANLTAVDTPDAHT
AILRFSKPSPVVMNALSGMESPILPKHIYENGDPLRNPANNAPIGTGPFKFVEWKRGSHIILERFDGYWAQERPYLDRIV
MRIINDAGARAAALETGEILLAGPNPVPYSELARFRANPKFDVETRGEELLQHAQAIEVNLRNPILAKVEVRQAILQAVN
REAMARAVWYQSGRPATSPIPYQAGDLRLANPPAYPFDVKKAEALLDAAGYPRGADKMRFKLRLDWLPLGEANLRAAEFI
KQSLQRVGIDITIRSSDLPTYLKTIYGDYDYDLNVFLYAPIFDPSMGLQRFYWSKAAKPGSPFVIASGYSSPEMDKILES
ASTEMDPAKRKALFHDFQRLAMTDLPVLPLLDLDYTAIISKRVHGVMDMPEGIRGSFENVWLSA
>Mature_543_residues
TSELDRLPFGPISRRLFLGGAGAAAATLFIGQNGKAFAAETGQKGGTLTMAVWPDPTALVCAFTSSDQVVLSSAKMTEGL
VAYGYDFLPRPRLATAWEPSADGLSIKFTLRQGVKWHDGTDFTSADVAFSFLNLLKPNHPRGRGTFANLTAVDTPDAHTA
ILRFSKPSPVVMNALSGMESPILPKHIYENGDPLRNPANNAPIGTGPFKFVEWKRGSHIILERFDGYWAQERPYLDRIVM
RIINDAGARAAALETGEILLAGPNPVPYSELARFRANPKFDVETRGEELLQHAQAIEVNLRNPILAKVEVRQAILQAVNR
EAMARAVWYQSGRPATSPIPYQAGDLRLANPPAYPFDVKKAEALLDAAGYPRGADKMRFKLRLDWLPLGEANLRAAEFIK
QSLQRVGIDITIRSSDLPTYLKTIYGDYDYDLNVFLYAPIFDPSMGLQRFYWSKAAKPGSPFVIASGYSSPEMDKILESA
STEMDPAKRKALFHDFQRLAMTDLPVLPLLDLDYTAIISKRVHGVMDMPEGIRGSFENVWLSA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=26.0869565217391, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789966, Length=477, Percent_Identity=27.6729559748428, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787762, Length=449, Percent_Identity=25.6124721603563, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=494, Percent_Identity=25.1012145748988, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787551, Length=454, Percent_Identity=23.3480176211454, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787495, Length=473, Percent_Identity=22.6215644820296, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081878, Length=485, Percent_Identity=23.2989690721649, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87082063, Length=430, Percent_Identity=24.1860465116279, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60036; Mature: 59905

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSELDRLPFGPISRRLFLGGAGAAAATLFIGQNGKAFAAETGQKGGTLTMAVWPDPTAL
CCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCEE
VCAFTSSDQVVLSSAKMTEGLVAYGYDFLPRPRLATAWEPSADGLSIKFTLRQGVKWHDG
EEEECCCCCEEEECCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEECCC
TDFTSADVAFSFLNLLKPNHPRGRGTFANLTAVDTPDAHTAILRFSKPSPVVMNALSGME
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCC
SPILPKHIYENGDPLRNPANNAPIGTGPFKFVEWKRGSHIILERFDGYWAQERPYLDRIV
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
MRIINDAGARAAALETGEILLAGPNPVPYSELARFRANPKFDVETRGEELLQHAQAIEVN
HHHHHCCCCCEEEEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEE
LRNPILAKVEVRQAILQAVNREAMARAVWYQSGRPATSPIPYQAGDLRLANPPAYPFDVK
CCCCEEEHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHH
KAEALLDAAGYPRGADKMRFKLRLDWLPLGEANLRAAEFIKQSLQRVGIDITIRSSDLPT
HHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCH
YLKTIYGDYDYDLNVFLYAPIFDPSMGLQRFYWSKAAKPGSPFVIASGYSSPEMDKILES
HHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
ASTEMDPAKRKALFHDFQRLAMTDLPVLPLLDLDYTAIISKRVHGVMDMPEGIRGSFENV
HCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WLSA
EECC
>Mature Secondary Structure 
TSELDRLPFGPISRRLFLGGAGAAAATLFIGQNGKAFAAETGQKGGTLTMAVWPDPTAL
CCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCEE
VCAFTSSDQVVLSSAKMTEGLVAYGYDFLPRPRLATAWEPSADGLSIKFTLRQGVKWHDG
EEEECCCCCEEEECCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEECCC
TDFTSADVAFSFLNLLKPNHPRGRGTFANLTAVDTPDAHTAILRFSKPSPVVMNALSGME
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCC
SPILPKHIYENGDPLRNPANNAPIGTGPFKFVEWKRGSHIILERFDGYWAQERPYLDRIV
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
MRIINDAGARAAALETGEILLAGPNPVPYSELARFRANPKFDVETRGEELLQHAQAIEVN
HHHHHCCCCCEEEEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEE
LRNPILAKVEVRQAILQAVNREAMARAVWYQSGRPATSPIPYQAGDLRLANPPAYPFDVK
CCCCEEEHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHH
KAEALLDAAGYPRGADKMRFKLRLDWLPLGEANLRAAEFIKQSLQRVGIDITIRSSDLPT
HHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCH
YLKTIYGDYDYDLNVFLYAPIFDPSMGLQRFYWSKAAKPGSPFVIASGYSSPEMDKILES
HHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
ASTEMDPAKRKALFHDFQRLAMTDLPVLPLLDLDYTAIISKRVHGVMDMPEGIRGSFENV
HCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WLSA
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA