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The map label for this gene is appF [H]

Identifier: 158424278

GI number: 158424278

Start: 3006762

End: 3007622

Strand: Direct

Name: appF [H]

Synonym: AZC_2654

Alternate gene names: 158424278

Gene position: 3006762-3007622 (Clockwise)

Preceding gene: 158424277

Following gene: 158424279

Centisome position: 55.99

GC content: 70.27

Gene sequence:

>861_bases
ATGGGCCTCGCCCCGGCCACCTCCGGCACGGTGCGGCTCGATGGCGAGGTGATCTCGGGTCTGCCCTATGCGCAGCTGCG
CCCGCTGCGGCGGCGCATGCAGATGGTGTTTCAGGACCCCTACGCCTCGCTCAATCCGCGCAAGCGCGTGGCGGACATCA
TCGCGGAACCGCTGCAGGTGCACGGCATCGGGACCGCGCAGGACCGGCGGGCCAAGGTGCAGGAGCTGATGGCGCGCGTG
GGCCTGCCGGTGGATGCGGGCGGACGCTTCCCGCACGAATTCTCCGGCGGCCAGCGCCAGCGCATCGGCATCGCTCGCGC
GCTCGCCCTTAATCCCGCGCTCATCGTTGCCGACGAGCCGGTCTCCGCGCTCGATGTGTCGGTGCAGGCGCAGGTCATCA
ACCTGATGGTGCGCCTCCAGAAGGAGATGGGGCTCTCCTATCTGTTCATCTCCCACGATCTGGCGGTGGTGCATTACATC
GCCGACCGCATCCTGGTGATGTATCTGGGCAAGATCGTCGAGGTGGCGGACCGGGCGCGGGTGTGGAAGCAGCCGCTCCA
TCCCTATACGGCGGGCCTTCTCGCCGCCCATCCGGCGCCAGACCCCGCCGGCGCCCACCGGCCGCGCGTGCGCATCACCG
GCGACATGCCGAGCCCCACCAACCCGCCCTCGGGCTGCCGCTTCCACACCCGCTGCCCGTTCGTGGAAAACCGCTGCACC
GCCGAGGCGCCCGCGCTTCGCGCCCTCCCCGATGGCCGTCAGGTGGCCTGCCACCGCGTAGAGGTGACCGGTGCAGGCGA
GGCCCGTTCCCCGTCCGAGATTCTCTCCCTTGAACCCATCACGGCGCCTGCGCCCGTCTGA

Upstream 100 bases:

>100_bases
GCGCGCCGTGGATGGCGTCAGCTTCTCCATCGCTGCTGGCGAGACGGTCGGCCTCGTGGGCGAATCCGGCTGCGGCAAGT
CCACGCTCGGCCGCACCCTC

Downstream 100 bases:

>100_bases
ACGAGACCCGTCATGACCTCAGAGCTTGATCGCCTGCCCTTCGGTCCCATCTCCCGCCGCCTGTTCCTTGGCGGCGCGGG
CGCTGCCGCCGCAACTTTGT

Product: oligopeptide ABC transporter ATP-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MGLAPATSGTVRLDGEVISGLPYAQLRPLRRRMQMVFQDPYASLNPRKRVADIIAEPLQVHGIGTAQDRRAKVQELMARV
GLPVDAGGRFPHEFSGGQRQRIGIARALALNPALIVADEPVSALDVSVQAQVINLMVRLQKEMGLSYLFISHDLAVVHYI
ADRILVMYLGKIVEVADRARVWKQPLHPYTAGLLAAHPAPDPAGAHRPRVRITGDMPSPTNPPSGCRFHTRCPFVENRCT
AEAPALRALPDGRQVACHRVEVTGAGEARSPSEILSLEPITAPAPV

Sequences:

>Translated_286_residues
MGLAPATSGTVRLDGEVISGLPYAQLRPLRRRMQMVFQDPYASLNPRKRVADIIAEPLQVHGIGTAQDRRAKVQELMARV
GLPVDAGGRFPHEFSGGQRQRIGIARALALNPALIVADEPVSALDVSVQAQVINLMVRLQKEMGLSYLFISHDLAVVHYI
ADRILVMYLGKIVEVADRARVWKQPLHPYTAGLLAAHPAPDPAGAHRPRVRITGDMPSPTNPPSGCRFHTRCPFVENRCT
AEAPALRALPDGRQVACHRVEVTGAGEARSPSEILSLEPITAPAPV
>Mature_285_residues
GLAPATSGTVRLDGEVISGLPYAQLRPLRRRMQMVFQDPYASLNPRKRVADIIAEPLQVHGIGTAQDRRAKVQELMARVG
LPVDAGGRFPHEFSGGQRQRIGIARALALNPALIVADEPVSALDVSVQAQVINLMVRLQKEMGLSYLFISHDLAVVHYIA
DRILVMYLGKIVEVADRARVWKQPLHPYTAGLLAAHPAPDPAGAHRPRVRITGDMPSPTNPPSGCRFHTRCPFVENRCTA
EAPALRALPDGRQVACHRVEVTGAGEARSPSEILSLEPITAPAPV

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG4608

COG function: function code E; ABC-type oligopeptide transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1787500, Length=263, Percent_Identity=50.9505703422053, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789962, Length=256, Percent_Identity=49.21875, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI145693107, Length=216, Percent_Identity=52.7777777777778, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1787758, Length=231, Percent_Identity=48.9177489177489, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1789963, Length=227, Percent_Identity=43.1718061674009, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI48994883, Length=266, Percent_Identity=39.8496240601504, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788506, Length=190, Percent_Identity=47.8947368421053, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787759, Length=253, Percent_Identity=39.1304347826087, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787547, Length=196, Percent_Identity=39.2857142857143, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787548, Length=213, Percent_Identity=35.6807511737089, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789891, Length=174, Percent_Identity=40.2298850574713, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789032, Length=185, Percent_Identity=37.8378378378378, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI48994997, Length=170, Percent_Identity=38.2352941176471, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787712, Length=202, Percent_Identity=34.6534653465347, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786398, Length=205, Percent_Identity=31.219512195122, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786872, Length=198, Percent_Identity=34.3434343434343, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788644, Length=164, Percent_Identity=32.9268292682927, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787370, Length=194, Percent_Identity=33.5051546391753, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=30.8755760368664, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788450, Length=188, Percent_Identity=33.5106382978723, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786563, Length=169, Percent_Identity=36.6863905325444, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787029, Length=184, Percent_Identity=33.1521739130435, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788761, Length=187, Percent_Identity=33.6898395721925, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788225, Length=191, Percent_Identity=34.0314136125654, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081791, Length=227, Percent_Identity=28.6343612334802, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789890, Length=205, Percent_Identity=32.6829268292683, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789873, Length=171, Percent_Identity=30.9941520467836, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787089, Length=161, Percent_Identity=34.7826086956522, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787164, Length=159, Percent_Identity=36.4779874213836, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082267, Length=197, Percent_Identity=28.4263959390863, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790467, Length=197, Percent_Identity=29.9492385786802, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081834, Length=173, Percent_Identity=33.5260115606936, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789672, Length=107, Percent_Identity=35.5140186915888, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790162, Length=187, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787105, Length=174, Percent_Identity=33.9080459770115, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48995001, Length=175, Percent_Identity=30.8571428571429, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786703, Length=179, Percent_Identity=29.0502793296089, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789586, Length=165, Percent_Identity=33.9393939393939, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786253, Length=172, Percent_Identity=27.3255813953488, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786345, Length=140, Percent_Identity=32.1428571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786981, Length=184, Percent_Identity=29.8913043478261, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790534, Length=134, Percent_Identity=29.8507462686567, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=173, Percent_Identity=30.635838150289, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=174, Percent_Identity=31.6091954022989, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 31040; Mature: 30909

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAPATSGTVRLDGEVISGLPYAQLRPLRRRMQMVFQDPYASLNPRKRVADIIAEPLQV
CCCCCCCCCEEEECCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHE
HGIGTAQDRRAKVQELMARVGLPVDAGGRFPHEFSGGQRQRIGIARALALNPALIVADEP
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
VSALDVSVQAQVINLMVRLQKEMGLSYLFISHDLAVVHYIADRILVMYLGKIVEVADRAR
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWKQPLHPYTAGLLAAHPAPDPAGAHRPRVRITGDMPSPTNPPSGCRFHTRCPFVENRCT
HHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCHHCCCC
AEAPALRALPDGRQVACHRVEVTGAGEARSPSEILSLEPITAPAPV
CCCCCHHCCCCCCCEEEEEEEEECCCCCCCCHHHEEECCCCCCCCC
>Mature Secondary Structure 
GLAPATSGTVRLDGEVISGLPYAQLRPLRRRMQMVFQDPYASLNPRKRVADIIAEPLQV
CCCCCCCCEEEECCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHE
HGIGTAQDRRAKVQELMARVGLPVDAGGRFPHEFSGGQRQRIGIARALALNPALIVADEP
ECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
VSALDVSVQAQVINLMVRLQKEMGLSYLFISHDLAVVHYIADRILVMYLGKIVEVADRAR
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWKQPLHPYTAGLLAAHPAPDPAGAHRPRVRITGDMPSPTNPPSGCRFHTRCPFVENRCT
HHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCHHCCCC
AEAPALRALPDGRQVACHRVEVTGAGEARSPSEILSLEPITAPAPV
CCCCCHHCCCCCCCEEEEEEEEECCCCCCCCHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]