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The map label for this gene is gatA [H]

Identifier: 158424257

GI number: 158424257

Start: 2969652

End: 2970980

Strand: Direct

Name: gatA [H]

Synonym: AZC_2633

Alternate gene names: 158424257

Gene position: 2969652-2970980 (Clockwise)

Preceding gene: 158424256

Following gene: 158424258

Centisome position: 55.3

GC content: 68.92

Gene sequence:

>1329_bases
ATGAGCACGACCGCCCCCTGCTTCCTCACCGCCGCGCAGGCCGCCGCCCGGATCAAGGCCGGCACGCTGACCAGCGAGGC
TTTGATCCGCTCCTGCCTCGAGCGCATCGGGGCGCGCGACCCTGCCGTAAAGGCATGGCTCCATGTGGACCCGGACCTGG
CCATCCGGCAGGCGCGCGAGCTGGACAAGCGCCCGCCCGCGGGGCCGCTCCACGGCATCCCCTTCGGCGTGAAGGACATC
ATGGACACGGCGGATATGCCGACCACGTACAATTCCCAGGAATGGCAGGACCACCGGCCGACGCGGGATGCGGCCTGCGT
AAACATTGTCCGCCAGGCCGGCGCCGTGATCCTCGGCAAGACCGACACGGTGGAATTCGCCTTCAACTCCCGCAAGGCCG
CGAGCCGCAACCCCTACAATCTCGCCCACACGCCCGGCGGCTCCTCCTCCGGCTCCGGAGCGGCGGTGGGCGACTATCAG
GTGCAGTGCGCCTTCGGCACCCAGACCGCCGGCTCCCACATTCGCCCCGCCTCCTTCAACGGCATCTATGCCATCAAGCC
CAGTTGGGGCACGGTGAGCCGCGAAGGCGTGCACATGATATCCGCCATCCTCGACACGGTGGGCTGGTATGGCCGCAGCG
TGGACGATCTGATCCTCGGCGCGCAGGCCTTCCGCCTGCCGGGCATGGAGAGCCTCGCCGCCCGGGGCGTGAAGGGACTG
AAGATCGGCTATTGCGAGACGCCCTATTGGTCGCGCGCCGAGACCGGCGCCCGCAAAGCCATGGCCGTGGCCGTCGAGCG
GCTGCAGCGGGCGGGCGTCATCGTCGAGGAAATCGCGCTCCCGGCGCATTTCGCGGACATAGCCGAAGCCCACACGATGA
TCATGTTCGGAGACGGGCGCGTCGCGCTCTATGACGAATATCTGCGACAGGGCGGTCGTCTCCACCCCGAACTGAAGCTC
ACCGTCGAGAACGGGCGCGGCGTGACGCCCGAGACGCTCGTCGCGGCCTATGACCTCGCCGACACCTGCCGCATGGAATT
CGATGCCATCGCAGCGGGTTATGACGCAGTGCTCGCGCCGGCTGCCACGGGCGAGGCCCCGAAGGGTCTCCACACGGTGG
GCGACTGGATCTTCAACGGCCTGTGGACCCTGCTGCACACGCCCTGCGTGGCCATCCCCTCAGTGCTGGGCGACATCGGC
CTGCCCGTGGGCGTCCAGTTGGTCGGTGGCCGTCTCTCCGACGCCCGCCTCCTGGCCGTCGCGCAGTCTCTTCAGCCGGT
GCTGGACGATTTCGCCGACACCCGCGCCACCCTGCTCGCCGCCGCCTGA

Upstream 100 bases:

>100_bases
CACAGACCGTGCGCGCGGCGGGCCTCGCCCTGCCGCGCCAGCCCTCGAAGGAAGACGAACCCGCCCATCTCTTCACCATG
ACGGCCCCGCGAGGCACCAA

Downstream 100 bases:

>100_bases
ACATTCGCGCACATCGGAAAGGGAATACCCATGGAACCCTGTTTCCTGACCGCCGCCGAGGCGGCCTCTGCCATACGGGC
CGGCACACTCACCAGCGAAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSTTAPCFLTAAQAAARIKAGTLTSEALIRSCLERIGARDPAVKAWLHVDPDLAIRQARELDKRPPAGPLHGIPFGVKDI
MDTADMPTTYNSQEWQDHRPTRDAACVNIVRQAGAVILGKTDTVEFAFNSRKAASRNPYNLAHTPGGSSSGSGAAVGDYQ
VQCAFGTQTAGSHIRPASFNGIYAIKPSWGTVSREGVHMISAILDTVGWYGRSVDDLILGAQAFRLPGMESLAARGVKGL
KIGYCETPYWSRAETGARKAMAVAVERLQRAGVIVEEIALPAHFADIAEAHTMIMFGDGRVALYDEYLRQGGRLHPELKL
TVENGRGVTPETLVAAYDLADTCRMEFDAIAAGYDAVLAPAATGEAPKGLHTVGDWIFNGLWTLLHTPCVAIPSVLGDIG
LPVGVQLVGGRLSDARLLAVAQSLQPVLDDFADTRATLLAAA

Sequences:

>Translated_442_residues
MSTTAPCFLTAAQAAARIKAGTLTSEALIRSCLERIGARDPAVKAWLHVDPDLAIRQARELDKRPPAGPLHGIPFGVKDI
MDTADMPTTYNSQEWQDHRPTRDAACVNIVRQAGAVILGKTDTVEFAFNSRKAASRNPYNLAHTPGGSSSGSGAAVGDYQ
VQCAFGTQTAGSHIRPASFNGIYAIKPSWGTVSREGVHMISAILDTVGWYGRSVDDLILGAQAFRLPGMESLAARGVKGL
KIGYCETPYWSRAETGARKAMAVAVERLQRAGVIVEEIALPAHFADIAEAHTMIMFGDGRVALYDEYLRQGGRLHPELKL
TVENGRGVTPETLVAAYDLADTCRMEFDAIAAGYDAVLAPAATGEAPKGLHTVGDWIFNGLWTLLHTPCVAIPSVLGDIG
LPVGVQLVGGRLSDARLLAVAQSLQPVLDDFADTRATLLAAA
>Mature_441_residues
STTAPCFLTAAQAAARIKAGTLTSEALIRSCLERIGARDPAVKAWLHVDPDLAIRQARELDKRPPAGPLHGIPFGVKDIM
DTADMPTTYNSQEWQDHRPTRDAACVNIVRQAGAVILGKTDTVEFAFNSRKAASRNPYNLAHTPGGSSSGSGAAVGDYQV
QCAFGTQTAGSHIRPASFNGIYAIKPSWGTVSREGVHMISAILDTVGWYGRSVDDLILGAQAFRLPGMESLAARGVKGLK
IGYCETPYWSRAETGARKAMAVAVERLQRAGVIVEEIALPAHFADIAEAHTMIMFGDGRVALYDEYLRQGGRLHPELKLT
VENGRGVTPETLVAAYDLADTCRMEFDAIAAGYDAVLAPAATGEAPKGLHTVGDWIFNGLWTLLHTPCVAIPSVLGDIGL
PVGVQLVGGRLSDARLLAVAQSLQPVLDDFADTRATLLAAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=516, Percent_Identity=25, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI195972892, Length=482, Percent_Identity=23.4439834024896, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=24.7464503042596, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=26.1574074074074, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=479, Percent_Identity=24.8434237995825, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=275, Percent_Identity=26.9090909090909, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356731, Length=464, Percent_Identity=24.3534482758621, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=24.5398773006135, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652985, Length=482, Percent_Identity=24.896265560166, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922090, Length=482, Percent_Identity=24.896265560166, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652981, Length=482, Percent_Identity=24.896265560166, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652983, Length=482, Percent_Identity=24.896265560166, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550774, Length=206, Percent_Identity=27.6699029126214, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=27.6699029126214, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=27.6699029126214, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47010; Mature: 46879

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTAPCFLTAAQAAARIKAGTLTSEALIRSCLERIGARDPAVKAWLHVDPDLAIRQARE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
LDKRPPAGPLHGIPFGVKDIMDTADMPTTYNSQEWQDHRPTRDAACVNIVRQAGAVILGK
HHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCEEEECC
TDTVEFAFNSRKAASRNPYNLAHTPGGSSSGSGAAVGDYQVQCAFGTQTAGSHIRPASFN
CCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCC
GIYAIKPSWGTVSREGVHMISAILDTVGWYGRSVDDLILGAQAFRLPGMESLAARGVKGL
CEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCC
KIGYCETPYWSRAETGARKAMAVAVERLQRAGVIVEEIALPAHFADIAEAHTMIMFGDGR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCEEEEECCCC
VALYDEYLRQGGRLHPELKLTVENGRGVTPETLVAAYDLADTCRMEFDAIAAGYDAVLAP
EEHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
AATGEAPKGLHTVGDWIFNGLWTLLHTPCVAIPSVLGDIGLPVGVQLVGGRLSDARLLAV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
AQSLQPVLDDFADTRATLLAAA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTAPCFLTAAQAAARIKAGTLTSEALIRSCLERIGARDPAVKAWLHVDPDLAIRQARE
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
LDKRPPAGPLHGIPFGVKDIMDTADMPTTYNSQEWQDHRPTRDAACVNIVRQAGAVILGK
HHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCEEEECC
TDTVEFAFNSRKAASRNPYNLAHTPGGSSSGSGAAVGDYQVQCAFGTQTAGSHIRPASFN
CCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCC
GIYAIKPSWGTVSREGVHMISAILDTVGWYGRSVDDLILGAQAFRLPGMESLAARGVKGL
CEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCC
KIGYCETPYWSRAETGARKAMAVAVERLQRAGVIVEEIALPAHFADIAEAHTMIMFGDGR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCEEEEECCCC
VALYDEYLRQGGRLHPELKLTVENGRGVTPETLVAAYDLADTCRMEFDAIAAGYDAVLAP
EEHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
AATGEAPKGLHTVGDWIFNGLWTLLHTPCVAIPSVLGDIGLPVGVQLVGGRLSDARLLAV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
AQSLQPVLDDFADTRATLLAAA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA