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The map label for this gene is gatA [H]

Identifier: 158424256

GI number: 158424256

Start: 2967991

End: 2969430

Strand: Direct

Name: gatA [H]

Synonym: AZC_2632

Alternate gene names: 158424256

Gene position: 2967991-2969430 (Clockwise)

Preceding gene: 158424255

Following gene: 158424257

Centisome position: 55.27

GC content: 68.12

Gene sequence:

>1440_bases
ATGAACGGGCCGAGCACCACGGCAGCCTTCAGCGAAGCCGCCACACCGCTGCATTTCCTAAGCATCACCGAGGCCTCGCG
GCTCATCGGTTCCGGCCGGCTGTCGCCGGTCACGCTCGTGGAGGCCTTCCTCGCCCGCATCGAGGCCATCGATGCGCGGC
TCAAGAGCTACATCACGATCACCCGAGCTCGCGCGCTGGCAGCTGCAAAAATCGCCGAGGCAGAGATTGCCGCCGGCCGC
TGGAAGGGGCCGCTGCACGGCATCCCCTTCGCGGTGAAGGACAATTACCACGTGGCCGGCCTGCCCACCACTGGCGGCTC
GCGCCTCATGCTCCATCACGTGGCCGATGCAACCTCCACGGCCGTGGCGCGGCTGGAAGCGGCGGGCGCGATCCTGCTCG
GCAAGCTCAACACCTGGGAATATGGCACCGGCAATGGCGGCGTCTACCATGACCTGCCCTTCGAGGTGGCACGCAATCCC
TGGGATCTCGCCTGCTTCACCGGCGGCTCCTCCACCGGTGCGGGTGCTGCCGTTGCGGCAGGCACCGCCATGTTCGCCCT
CGGCTCGGATACCGGCGGCTCCATCCGCCTGCCAGCCGCAGCCTGCGGCCTCCAGGGCTTCAAGGCCACCTTCGGTCGCG
TCAGCCGCGCCCACTGCCTGCCCAATTGCTGGACGCTCGACCACACCGGCCCGCTCACCTGGACGGTGGAGGACAATGCC
ATCGTCATGCAGGCCATCTCCGGCTTCGATCCGGGGGATCCCTCCGCCGCCGACGTGCCCGTGCCGGATTATCGCAAGAA
CCTGCATGCGGGCGTGAAGGGGCTGGTGATCGGCTTCGTCCGCGACCTCGGCCCGGAAGGGGCAGCTCTTGATGCCGCCA
ATGCGGCAGCGCTGCAGGAGGCGGCCCGCGTACTGGAGGCACAGGGCGCCATCATCCGCGAGGTCACCCTGCCGGCCCCT
CTGGCGCAGTACCGGGCGGTGACAAAGGTGATCAACTGGTCGGAATCCTATTCCATCCATGAGCAGGATTTTCTGGAGCG
TAGCGCCCTCATGGGGCAGGCCCTGCGTGAGAAGATGATGTCGGGCTTCACGGTCCGCGCCGCCGATTATCTCGCCGCCA
CCCGCCTGCGCCGCTCCCTCGTGGACACCACCGACGCGCTGGTGCGCTCCTGCGACGCCCTTCTGATCGCAGGCGCCTTC
CATGTGGCCCCGCGCTTTGATGCGCCGGACACCATCACCCCCTTCACCGCGGACACGGCCACCACGGTGTTCAACGTGAC
CGGCCATCCAGCCCTGTCGATCTGCACCGGCTTTGACGCCAGTGGCCTGCCTCTGAATGCCCAGATCGTCGGCCGCTTCT
TCGACGAAGAGACCGTGCTGCGCGTGGCCCAGTCCTACGAGGCCGCCACCCCGTGGCGCGCCCGTCGGCCGACGCTGTGA

Upstream 100 bases:

>100_bases
TCGGCGCCCTACCTGTTCCGCCTGCTCCGGGCGACGGGCGTAATGCCGATGCCCCCCACGCACGCCGCCGCGCGCCTGCC
GGCCGAATGAGAGGTGTTTC

Downstream 100 bases:

>100_bases
ACCCGGGAGCGACCCATGTCAGACCTCCAGACGCCGACCCTTGAAGGAATCCGCCTCTATGCCGCGCGACACGGGCTGAA
GGATCTGCCGGATGACCAGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNGPSTTAAFSEAATPLHFLSITEASRLIGSGRLSPVTLVEAFLARIEAIDARLKSYITITRARALAAAKIAEAEIAAGR
WKGPLHGIPFAVKDNYHVAGLPTTGGSRLMLHHVADATSTAVARLEAAGAILLGKLNTWEYGTGNGGVYHDLPFEVARNP
WDLACFTGGSSTGAGAAVAAGTAMFALGSDTGGSIRLPAAACGLQGFKATFGRVSRAHCLPNCWTLDHTGPLTWTVEDNA
IVMQAISGFDPGDPSAADVPVPDYRKNLHAGVKGLVIGFVRDLGPEGAALDAANAAALQEAARVLEAQGAIIREVTLPAP
LAQYRAVTKVINWSESYSIHEQDFLERSALMGQALREKMMSGFTVRAADYLAATRLRRSLVDTTDALVRSCDALLIAGAF
HVAPRFDAPDTITPFTADTATTVFNVTGHPALSICTGFDASGLPLNAQIVGRFFDEETVLRVAQSYEAATPWRARRPTL

Sequences:

>Translated_479_residues
MNGPSTTAAFSEAATPLHFLSITEASRLIGSGRLSPVTLVEAFLARIEAIDARLKSYITITRARALAAAKIAEAEIAAGR
WKGPLHGIPFAVKDNYHVAGLPTTGGSRLMLHHVADATSTAVARLEAAGAILLGKLNTWEYGTGNGGVYHDLPFEVARNP
WDLACFTGGSSTGAGAAVAAGTAMFALGSDTGGSIRLPAAACGLQGFKATFGRVSRAHCLPNCWTLDHTGPLTWTVEDNA
IVMQAISGFDPGDPSAADVPVPDYRKNLHAGVKGLVIGFVRDLGPEGAALDAANAAALQEAARVLEAQGAIIREVTLPAP
LAQYRAVTKVINWSESYSIHEQDFLERSALMGQALREKMMSGFTVRAADYLAATRLRRSLVDTTDALVRSCDALLIAGAF
HVAPRFDAPDTITPFTADTATTVFNVTGHPALSICTGFDASGLPLNAQIVGRFFDEETVLRVAQSYEAATPWRARRPTL
>Mature_479_residues
MNGPSTTAAFSEAATPLHFLSITEASRLIGSGRLSPVTLVEAFLARIEAIDARLKSYITITRARALAAAKIAEAEIAAGR
WKGPLHGIPFAVKDNYHVAGLPTTGGSRLMLHHVADATSTAVARLEAAGAILLGKLNTWEYGTGNGGVYHDLPFEVARNP
WDLACFTGGSSTGAGAAVAAGTAMFALGSDTGGSIRLPAAACGLQGFKATFGRVSRAHCLPNCWTLDHTGPLTWTVEDNA
IVMQAISGFDPGDPSAADVPVPDYRKNLHAGVKGLVIGFVRDLGPEGAALDAANAAALQEAARVLEAQGAIIREVTLPAP
LAQYRAVTKVINWSESYSIHEQDFLERSALMGQALREKMMSGFTVRAADYLAATRLRRSLVDTTDALVRSCDALLIAGAF
HVAPRFDAPDTITPFTADTATTVFNVTGHPALSICTGFDASGLPLNAQIVGRFFDEETVLRVAQSYEAATPWRARRPTL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=32.2772277227723, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI195972892, Length=510, Percent_Identity=25.4901960784314, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=27.5779376498801, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=490, Percent_Identity=25.5102040816327, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=28.2051282051282, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=311, Percent_Identity=27.0096463022508, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=240, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=305, Percent_Identity=27.2131147540984, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=305, Percent_Identity=27.2131147540984, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=25.9345794392523, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319685, Length=391, Percent_Identity=27.3657289002558, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=28.0487804878049, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=26.2, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=479, Percent_Identity=25.2609603340292, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=24.2603550295858, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=23.6734693877551, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=23.6734693877551, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550774, Length=490, Percent_Identity=23.6734693877551, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=24.1545893719807, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=24.1545893719807, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652985, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922090, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652981, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652983, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50494; Mature: 50494

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPSTTAAFSEAATPLHFLSITEASRLIGSGRLSPVTLVEAFLARIEAIDARLKSYITI
CCCCCCHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRARALAAAKIAEAEIAAGRWKGPLHGIPFAVKDNYHVAGLPTTGGSRLMLHHVADATST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEEEHHHHHHH
AVARLEAAGAILLGKLNTWEYGTGNGGVYHDLPFEVARNPWDLACFTGGSSTGAGAAVAA
HHHHHHHCCEEEEECCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEECCCCCCCCHHHHH
GTAMFALGSDTGGSIRLPAAACGLQGFKATFGRVSRAHCLPNCWTLDHTGPLTWTVEDNA
CEEEEEECCCCCCCEECCHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCE
IVMQAISGFDPGDPSAADVPVPDYRKNLHAGVKGLVIGFVRDLGPEGAALDAANAAALQE
EEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AARVLEAQGAIIREVTLPAPLAQYRAVTKVINWSESYSIHEQDFLERSALMGQALREKMM
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGFTVRAADYLAATRLRRSLVDTTDALVRSCDALLIAGAFHVAPRFDAPDTITPFTADTA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCCCCCCCC
TTVFNVTGHPALSICTGFDASGLPLNAQIVGRFFDEETVLRVAQSYEAATPWRARRPTL
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGPSTTAAFSEAATPLHFLSITEASRLIGSGRLSPVTLVEAFLARIEAIDARLKSYITI
CCCCCCHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRARALAAAKIAEAEIAAGRWKGPLHGIPFAVKDNYHVAGLPTTGGSRLMLHHVADATST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEEEHHHHHHH
AVARLEAAGAILLGKLNTWEYGTGNGGVYHDLPFEVARNPWDLACFTGGSSTGAGAAVAA
HHHHHHHCCEEEEECCCCCCCCCCCCCEEECCCHHHCCCCCEEEEEECCCCCCCCHHHHH
GTAMFALGSDTGGSIRLPAAACGLQGFKATFGRVSRAHCLPNCWTLDHTGPLTWTVEDNA
CEEEEEECCCCCCCEECCHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCE
IVMQAISGFDPGDPSAADVPVPDYRKNLHAGVKGLVIGFVRDLGPEGAALDAANAAALQE
EEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AARVLEAQGAIIREVTLPAPLAQYRAVTKVINWSESYSIHEQDFLERSALMGQALREKMM
HHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGFTVRAADYLAATRLRRSLVDTTDALVRSCDALLIAGAFHVAPRFDAPDTITPFTADTA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCCCCCCCC
TTVFNVTGHPALSICTGFDASGLPLNAQIVGRFFDEETVLRVAQSYEAATPWRARRPTL
EEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA