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The map label for this gene is gatA [H]

Identifier: 158424248

GI number: 158424248

Start: 2957879

End: 2959306

Strand: Direct

Name: gatA [H]

Synonym: AZC_2624

Alternate gene names: 158424248

Gene position: 2957879-2959306 (Clockwise)

Preceding gene: 158424247

Following gene: 158424249

Centisome position: 55.08

GC content: 67.86

Gene sequence:

>1428_bases
ATGAGCGCGCTCCATGAAATGACCATCGCCGAGGCCGGCCGGCAGTTTCGAGCCGGCACGCTGTCGCCGGTGGACCTGAC
CGAAGCCTTTCTGGCGCGCATGGATGCGCTCGATGGCGCCCTCAACAGTTTCATCACCATCACGGCGGAGCGGGCCCGCA
CGGAGGCAAAGGACGCCGAAACGCTGTTTGCACGGGGCGTCGACAAGGGCCCCATGCAAGGCGCTCCCTACGGTTTGAAG
GACATTTACGACACGGCCGGCGTTCGCACCACCGCCCATTCAAAGCTGCTGGAGCACCACGTCCCGACGGAGGACTGCAC
GGTACAGGCGAAGCTGCGGGCCGGCGGCGGCGTGTTGCTGGGCAAGCAGGCCACTTGGGAATTCGCCATGGGCGGCCCCT
CGTTCGACCTGCCTTGGCCGCCAGCGCGCAATCCGTGGGACCTCGCTCGCTCGCCCCTGGGTTCATCCAGCGGCGCGGGT
GCCTCCATCGCGGCAGGCCTCTGTCCGGCTGCAACAGGCAGGGATACCGGCGGCTCGATCCGTATGCCCGCGGCTGCCTG
CGGGATCGCCGGTCTCAAGCCAACCTATGGGCTGGTCAGCCGTCGGGGCGTGCAGCCCAACTCCTATTCTTTTGACCATT
GCGGCCCGATGGCCTGGACGGTGGAAGACTGCGCTCTGATGCTCGGCGTCATGGCCGGGCACGATCCGCTTGACCCCGCA
TCCATCGCCTTTCCCGCCGAGGACTATGCCGCCTGGCTCTTCACGCCCGTTGCCGGGCTGCGGATCGGCGTGGTGCGCCA
CTGGTACGAGGACGAGCTGGAAGCGGCACCGGCGGTGGTCGCCGCCATGGATGCAAGCATCGCGCTGTTCCACCGCCTCG
GTTGCCACATCAGCGAGGTGCGCCTTCCCAGCCTGCGCACCTTCACGGATTCCAAGATGCCGCTCACGGCGGGCGAGATT
TTCTCCCTGCATGAGATGGATCTGCGCACGCGGCCTCAGGATTTCGGCCGCTCCATGCGCTATCGCATCCTGCCCGGCGC
CCTGCTGCGGGCGGAAGACTATGTTCAGGCCATGCGGGCACGCGCAGAGCTCACCGCGCAGGTGTTCGAGGTGATGAGGG
AGGTCGATATCCTCCTCACGGCCACCAGCGCCCAGCCCGCCCCTCTCCTGCTGCCCGATGAGCCGCCCACCTTCTTCGGT
GCGAAGGTGCCCTCGCTCACGGCCCAGTTCAACCTCACCGGCCAGCCGGCCCTGTCGCTTTGCAACGGCTTCGACGTCGC
AGGCCTGCCGCTCGGCCTGCAGCTCGCTGGGCGACCGGGCGAAGACGCGCTTGTCCTGCGCCTCGGGGCTGCGTTCGAGC
GGGAAACCGATTTCCGCCGCCACCGGCCGTCGCTGCACCAGCCGGAGGAGGCCATGGCCGCGCAATGA

Upstream 100 bases:

>100_bases
CGAGCCCATGCTGATGCGCCTGCGGCGGGGCCGAGACCGGGCTGACGAGCCGGCGCATGTATTTGATCCGCGCAAGTTCA
TGCCGCAGACGGCCTGAGCC

Downstream 100 bases:

>100_bases
TGATGAGTGGCCGCATAAGGCCGCCGCCGATCGGGGAACGCACGACGAGCCACCGGACAAGAGCAGGGAAGATTCCGTCA
ATGAACAGACGTTCGTTTAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSALHEMTIAEAGRQFRAGTLSPVDLTEAFLARMDALDGALNSFITITAERARTEAKDAETLFARGVDKGPMQGAPYGLK
DIYDTAGVRTTAHSKLLEHHVPTEDCTVQAKLRAGGGVLLGKQATWEFAMGGPSFDLPWPPARNPWDLARSPLGSSSGAG
ASIAAGLCPAATGRDTGGSIRMPAAACGIAGLKPTYGLVSRRGVQPNSYSFDHCGPMAWTVEDCALMLGVMAGHDPLDPA
SIAFPAEDYAAWLFTPVAGLRIGVVRHWYEDELEAAPAVVAAMDASIALFHRLGCHISEVRLPSLRTFTDSKMPLTAGEI
FSLHEMDLRTRPQDFGRSMRYRILPGALLRAEDYVQAMRARAELTAQVFEVMREVDILLTATSAQPAPLLLPDEPPTFFG
AKVPSLTAQFNLTGQPALSLCNGFDVAGLPLGLQLAGRPGEDALVLRLGAAFERETDFRRHRPSLHQPEEAMAAQ

Sequences:

>Translated_475_residues
MSALHEMTIAEAGRQFRAGTLSPVDLTEAFLARMDALDGALNSFITITAERARTEAKDAETLFARGVDKGPMQGAPYGLK
DIYDTAGVRTTAHSKLLEHHVPTEDCTVQAKLRAGGGVLLGKQATWEFAMGGPSFDLPWPPARNPWDLARSPLGSSSGAG
ASIAAGLCPAATGRDTGGSIRMPAAACGIAGLKPTYGLVSRRGVQPNSYSFDHCGPMAWTVEDCALMLGVMAGHDPLDPA
SIAFPAEDYAAWLFTPVAGLRIGVVRHWYEDELEAAPAVVAAMDASIALFHRLGCHISEVRLPSLRTFTDSKMPLTAGEI
FSLHEMDLRTRPQDFGRSMRYRILPGALLRAEDYVQAMRARAELTAQVFEVMREVDILLTATSAQPAPLLLPDEPPTFFG
AKVPSLTAQFNLTGQPALSLCNGFDVAGLPLGLQLAGRPGEDALVLRLGAAFERETDFRRHRPSLHQPEEAMAAQ
>Mature_474_residues
SALHEMTIAEAGRQFRAGTLSPVDLTEAFLARMDALDGALNSFITITAERARTEAKDAETLFARGVDKGPMQGAPYGLKD
IYDTAGVRTTAHSKLLEHHVPTEDCTVQAKLRAGGGVLLGKQATWEFAMGGPSFDLPWPPARNPWDLARSPLGSSSGAGA
SIAAGLCPAATGRDTGGSIRMPAAACGIAGLKPTYGLVSRRGVQPNSYSFDHCGPMAWTVEDCALMLGVMAGHDPLDPAS
IAFPAEDYAAWLFTPVAGLRIGVVRHWYEDELEAAPAVVAAMDASIALFHRLGCHISEVRLPSLRTFTDSKMPLTAGEIF
SLHEMDLRTRPQDFGRSMRYRILPGALLRAEDYVQAMRARAELTAQVFEVMREVDILLTATSAQPAPLLLPDEPPTFFGA
KVPSLTAQFNLTGQPALSLCNGFDVAGLPLGLQLAGRPGEDALVLRLGAAFERETDFRRHRPSLHQPEEAMAAQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=30.060120240481, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.748490945674, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=28.8939051918736, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=24.9500998003992, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=292, Percent_Identity=26.027397260274, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=465, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=383, Percent_Identity=26.6318537859008, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=33.9357429718875, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=25.3061224489796, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24644968, Length=495, Percent_Identity=24.8484848484848, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550774, Length=483, Percent_Identity=23.3954451345756, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648435, Length=484, Percent_Identity=23.5537190082645, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648437, Length=484, Percent_Identity=23.5537190082645, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=26.1316872427983, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=26.1316872427983, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=26.1316872427983, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=26.1316872427983, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648441, Length=407, Percent_Identity=24.3243243243243, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=407, Percent_Identity=24.3243243243243, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356731, Length=233, Percent_Identity=27.0386266094421, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 50944; Mature: 50813

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALHEMTIAEAGRQFRAGTLSPVDLTEAFLARMDALDGALNSFITITAERARTEAKDAE
CCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHH
TLFARGVDKGPMQGAPYGLKDIYDTAGVRTTAHSKLLEHHVPTEDCTVQAKLRAGGGVLL
HHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
GKQATWEFAMGGPSFDLPWPPARNPWDLARSPLGSSSGAGASIAAGLCPAATGRDTGGSI
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCE
RMPAAACGIAGLKPTYGLVSRRGVQPNSYSFDHCGPMAWTVEDCALMLGVMAGHDPLDPA
ECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
SIAFPAEDYAAWLFTPVAGLRIGVVRHWYEDELEAAPAVVAAMDASIALFHRLGCHISEV
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEE
RLPSLRTFTDSKMPLTAGEIFSLHEMDLRTRPQDFGRSMRYRILPGALLRAEDYVQAMRA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHH
RAELTAQVFEVMREVDILLTATSAQPAPLLLPDEPPTFFGAKVPSLTAQFNLTGQPALSL
HHHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCHHHHH
CNGFDVAGLPLGLQLAGRPGEDALVLRLGAAFERETDFRRHRPSLHQPEEAMAAQ
HCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHCCC
>Mature Secondary Structure 
SALHEMTIAEAGRQFRAGTLSPVDLTEAFLARMDALDGALNSFITITAERARTEAKDAE
CHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHH
TLFARGVDKGPMQGAPYGLKDIYDTAGVRTTAHSKLLEHHVPTEDCTVQAKLRAGGGVLL
HHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
GKQATWEFAMGGPSFDLPWPPARNPWDLARSPLGSSSGAGASIAAGLCPAATGRDTGGSI
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCE
RMPAAACGIAGLKPTYGLVSRRGVQPNSYSFDHCGPMAWTVEDCALMLGVMAGHDPLDPA
ECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCC
SIAFPAEDYAAWLFTPVAGLRIGVVRHWYEDELEAAPAVVAAMDASIALFHRLGCHISEV
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEE
RLPSLRTFTDSKMPLTAGEIFSLHEMDLRTRPQDFGRSMRYRILPGALLRAEDYVQAMRA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHH
RAELTAQVFEVMREVDILLTATSAQPAPLLLPDEPPTFFGAKVPSLTAQFNLTGQPALSL
HHHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCHHHHH
CNGFDVAGLPLGLQLAGRPGEDALVLRLGAAFERETDFRRHRPSLHQPEEAMAAQ
HCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA