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The map label for this gene is gatA [H]

Identifier: 158424247

GI number: 158424247

Start: 2956153

End: 2957586

Strand: Direct

Name: gatA [H]

Synonym: AZC_2623

Alternate gene names: 158424247

Gene position: 2956153-2957586 (Clockwise)

Preceding gene: 158424245

Following gene: 158424248

Centisome position: 55.05

GC content: 67.02

Gene sequence:

>1434_bases
ATGGCTGCTCTCGAACTCGCGGCGCGCGAACGCGTCGATGTCGGAGACACTGATCTCGTCACTGCGGGCGTCCTAGAACT
CGGTCGAATGATCCGCACCGGCGAGACCTCCCCGGTCGAGATCACGAAGGCCTGCCTTTCCCGCATTGAGCGGATCGACA
GCAAGCTGAACAGCTTCATTACGGTCACTGCGGAGGCGGCCCTCGCGCAGGCGGCCGCCGTAGAGGCCGAACTCGCCGCC
GGTCTGGATCGCGGCCCCTTCCACGGCATACCCTACGCCCTGAAGGACAATGTCGATACGGCAGGCGTTCTGTCCTCCTC
CCACTCCAGGATCTTCATCGATCGTGTCCCGCAGGAGAGCGCGACTGTGGCGCGCAAGCTGGAGGCGGCCGGCGGCATTC
TCATCGGCAAGACGGCCACGTTCGAGTTCGCGATCGGCGGCCCATCCTTCGATCTGCCTTGGCCCCCGGCCCGGAATCCC
TGGAACCCCGACTATCTGCCCGGAGGTTCCTCGTCCGGCTCCGGCGCTGCGGTGGGCGGTCGCCTCGTCCCGGCCGCCAT
CGGCACTGATACCGGCGGCTCCATCCGCTGGCCGGCTGCCATGTGCGGCATCACCGGGCTGAAGCCCACCTATGGGCGCA
TCAGCCGGCGCGGTGTTCATCCCAACACCTTTTCGCTGGACCATTGCGGACCCATGGCCCGCTCGGCGGCGGACTGCGCC
GCCATGCTCGGTGCCTGCGCGGGCTATGATCCGCTCGATCCTGGTTCCATCGACGCGCCCGTGCCCGATTACACGGCTGC
GCTCACGGGCTCCATCAAGGGCCTGCGGATCGGTCTCGTGCGCCATTGGTACGAGGAGGAGGCGACCGACGAGGTGATCG
CCGCCGTGGATGATGCCGTGGAGGTGCTACGCCAGCTCGGCGCCGTGGTGGAACCGCTCACCCTCGATCCGCTGCGTGAT
TACGTCGACGCCAAGACCACCATTTCCATCACCGAGCTGTTCGCCGTGCATGAGGCGGACATCCGCTTCCGGCCGCAGGA
TTTCGGCCGGCTCTTGCGCAGTCGCGTCATGGTGGGCGGTCTCGTCCGCGCGGAGGATTATGTCCAGGCCATGCGCTGGC
GCGCGGAGCTCTCCACGCGCATGATGGCCGCCTTCTCGCGTTATGATCTTCTGGTCACCGCCGGCTGGATGGCGCCCGCC
GATCCGGCGGTGCCCGACGGCGTCGATTTCTTCAAGAAGCGTCAGCTCGTCACCATGCCCTTCAGCCTTGCGGGGCTCCC
GGCGCTTGCCCTGCCGTGCGGGTTTTCCAGCAAGGGCCTGCCGCTCTCGCTGCAGATCGCCGGTCGGCCGTTCGATGAGG
CCACTGTCTTGCGCGCCGGAGATGCCTACCAGCAGGCCACCGAATGGCACCTTGCTGTGCCGAACCTCGACTGA

Upstream 100 bases:

>100_bases
TGTTGGCACGGTGATTGCTTGACCAACAGGACACGATCTCGCGGCTGCCTCTGGCTGCGCATTACGAGATTGTATACAAT
ATCGCGAAAGGTCGAAGCGC

Downstream 100 bases:

>100_bases
CCACCCGACCGACGCGCACCGCGAGGACGCCAGCGTCCTCCCCTGTCATCGAACGGAGCCCAAGACGGCCATGAGCATCC
CCGTTCCACCTCGTCCCGCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAALELAARERVDVGDTDLVTAGVLELGRMIRTGETSPVEITKACLSRIERIDSKLNSFITVTAEAALAQAAAVEAELAA
GLDRGPFHGIPYALKDNVDTAGVLSSSHSRIFIDRVPQESATVARKLEAAGGILIGKTATFEFAIGGPSFDLPWPPARNP
WNPDYLPGGSSSGSGAAVGGRLVPAAIGTDTGGSIRWPAAMCGITGLKPTYGRISRRGVHPNTFSLDHCGPMARSAADCA
AMLGACAGYDPLDPGSIDAPVPDYTAALTGSIKGLRIGLVRHWYEEEATDEVIAAVDDAVEVLRQLGAVVEPLTLDPLRD
YVDAKTTISITELFAVHEADIRFRPQDFGRLLRSRVMVGGLVRAEDYVQAMRWRAELSTRMMAAFSRYDLLVTAGWMAPA
DPAVPDGVDFFKKRQLVTMPFSLAGLPALALPCGFSSKGLPLSLQIAGRPFDEATVLRAGDAYQQATEWHLAVPNLD

Sequences:

>Translated_477_residues
MAALELAARERVDVGDTDLVTAGVLELGRMIRTGETSPVEITKACLSRIERIDSKLNSFITVTAEAALAQAAAVEAELAA
GLDRGPFHGIPYALKDNVDTAGVLSSSHSRIFIDRVPQESATVARKLEAAGGILIGKTATFEFAIGGPSFDLPWPPARNP
WNPDYLPGGSSSGSGAAVGGRLVPAAIGTDTGGSIRWPAAMCGITGLKPTYGRISRRGVHPNTFSLDHCGPMARSAADCA
AMLGACAGYDPLDPGSIDAPVPDYTAALTGSIKGLRIGLVRHWYEEEATDEVIAAVDDAVEVLRQLGAVVEPLTLDPLRD
YVDAKTTISITELFAVHEADIRFRPQDFGRLLRSRVMVGGLVRAEDYVQAMRWRAELSTRMMAAFSRYDLLVTAGWMAPA
DPAVPDGVDFFKKRQLVTMPFSLAGLPALALPCGFSSKGLPLSLQIAGRPFDEATVLRAGDAYQQATEWHLAVPNLD
>Mature_476_residues
AALELAARERVDVGDTDLVTAGVLELGRMIRTGETSPVEITKACLSRIERIDSKLNSFITVTAEAALAQAAAVEAELAAG
LDRGPFHGIPYALKDNVDTAGVLSSSHSRIFIDRVPQESATVARKLEAAGGILIGKTATFEFAIGGPSFDLPWPPARNPW
NPDYLPGGSSSGSGAAVGGRLVPAAIGTDTGGSIRWPAAMCGITGLKPTYGRISRRGVHPNTFSLDHCGPMARSAADCAA
MLGACAGYDPLDPGSIDAPVPDYTAALTGSIKGLRIGLVRHWYEEEATDEVIAAVDDAVEVLRQLGAVVEPLTLDPLRDY
VDAKTTISITELFAVHEADIRFRPQDFGRLLRSRVMVGGLVRAEDYVQAMRWRAELSTRMMAAFSRYDLLVTAGWMAPAD
PAVPDGVDFFKKRQLVTMPFSLAGLPALALPCGFSSKGLPLSLQIAGRPFDEATVLRAGDAYQQATEWHLAVPNLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=30.5220883534137, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI195972892, Length=483, Percent_Identity=25.2587991718427, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17543272, Length=427, Percent_Identity=30.4449648711944, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17537465, Length=260, Percent_Identity=31.9230769230769, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=391, Percent_Identity=23.7851662404092, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=298, Percent_Identity=25.8389261744966, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=317, Percent_Identity=23.0283911671924, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=25.5411255411255, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319685, Length=390, Percent_Identity=27.9487179487179, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648113, Length=467, Percent_Identity=31.2633832976445, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=24.5579567779961, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356731, Length=239, Percent_Identity=32.2175732217573, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=24.0325865580448, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648435, Length=278, Percent_Identity=25.5395683453237, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648437, Length=278, Percent_Identity=25.5395683453237, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550774, Length=278, Percent_Identity=25.5395683453237, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652985, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648439, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50726; Mature: 50595

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALELAARERVDVGDTDLVTAGVLELGRMIRTGETSPVEITKACLSRIERIDSKLNSFI
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEE
TVTAEAALAQAAAVEAELAAGLDRGPFHGIPYALKDNVDTAGVLSSSHSRIFIDRVPQES
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHCCCCCEEEEECCCCHH
ATVARKLEAAGGILIGKTATFEFAIGGPSFDLPWPPARNPWNPDYLPGGSSSGSGAAVGG
HHHHHHHHHCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RLVPAAIGTDTGGSIRWPAAMCGITGLKPTYGRISRRGVHPNTFSLDHCGPMARSAADCA
EEEEHEECCCCCCCEECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AMLGACAGYDPLDPGSIDAPVPDYTAALTGSIKGLRIGLVRHWYEEEATDEVIAAVDDAV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
EVLRQLGAVVEPLTLDPLRDYVDAKTTISITELFAVHEADIRFRPQDFGRLLRSRVMVGG
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEECHHHHHHHHHHHHHHHH
LVRAEDYVQAMRWRAELSTRMMAAFSRYDLLVTAGWMAPADPAVPDGVDFFKKRQLVTMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECC
FSLAGLPALALPCGFSSKGLPLSLQIAGRPFDEATVLRAGDAYQQATEWHLAVPNLD
HHHHCCCHHEECCCCCCCCCEEEEEECCCCCCCHHEEECCCHHHHCCCEEEECCCCC
>Mature Secondary Structure 
AALELAARERVDVGDTDLVTAGVLELGRMIRTGETSPVEITKACLSRIERIDSKLNSFI
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEE
TVTAEAALAQAAAVEAELAAGLDRGPFHGIPYALKDNVDTAGVLSSSHSRIFIDRVPQES
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHCCCCCEEEEECCCCHH
ATVARKLEAAGGILIGKTATFEFAIGGPSFDLPWPPARNPWNPDYLPGGSSSGSGAAVGG
HHHHHHHHHCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RLVPAAIGTDTGGSIRWPAAMCGITGLKPTYGRISRRGVHPNTFSLDHCGPMARSAADCA
EEEEHEECCCCCCCEECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
AMLGACAGYDPLDPGSIDAPVPDYTAALTGSIKGLRIGLVRHWYEEEATDEVIAAVDDAV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
EVLRQLGAVVEPLTLDPLRDYVDAKTTISITELFAVHEADIRFRPQDFGRLLRSRVMVGG
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEECHHHHHHHHHHHHHHHH
LVRAEDYVQAMRWRAELSTRMMAAFSRYDLLVTAGWMAPADPAVPDGVDFFKKRQLVTMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECC
FSLAGLPALALPCGFSSKGLPLSLQIAGRPFDEATVLRAGDAYQQATEWHLAVPNLD
HHHHCCCHHEECCCCCCCCCEEEEEECCCCCCCHHEEECCCHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA