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The map label for this gene is gatA [H]

Identifier: 158424202

GI number: 158424202

Start: 2904345

End: 2905832

Strand: Reverse

Name: gatA [H]

Synonym: AZC_2578

Alternate gene names: 158424202

Gene position: 2905832-2904345 (Counterclockwise)

Preceding gene: 158424203

Following gene: 158424200

Centisome position: 54.11

GC content: 71.57

Gene sequence:

>1488_bases
TTGAGCATGACCCGTTCCGACACCATCCTGCCGGCGCCCGAGGCGCTCGCCTTCGCCTCGGCCATCGATCTGCTGGAGGC
TCTGCGGCGCGGCGACGTCACCTCCCGCGCCCTGCTGGAGCTCTATCTCGGCCGGATCGAGCGCCACAATCCGGCGCTGA
ATGCGGTGATCTTTCTGGAGGCCGAGGCTGCCCGTGCTCGTGCGGACGCCGCCGACGCCGCCCGCGCCCGCGGCGAGAGC
TGGGGGCCGCTGCACGGCCTGCCCATGACGGTGAAGGAATCCCACCATATCGCCGGCTGGCCCACCACCTGGGGCGATCC
GGCCACCGCCGATTTCCGGCCGGAGGCGACGGGCGTCGTGGTGCAGCGGCTTCTCGATGCCGGGGCCATCGTCTTCGGCA
AGACCAATGTGCCGATTCATCTCCTGGACTGGCAGAGCTACAACGCCATCCATGGCACCACGCACAATCCCTGGCGGCGG
GGCGTGACGCCGGGCGGCTCCTCGGGCGGCTCGGCGGTGGCGCTGGCGGCGGGCTTCACGGCGGCCGAGCTGGGGAGCGA
TGCGGGCGGCTCCGTGCGCATGCCCGCCCATTTCTGCGGCGTGTTCGGCCACCGGCCCAGCATTCATGTGGTGCCGCAGG
CGGGCAATGACCGGCCGGGCAGCACCATTGGCAACGAGGTCTCGACCTCCGGCCCCATGGCGCGTTCGGCGGGCGACCTC
GACCTGCTGCTCGGCATCCTCGCCGGCCCGGCCGGGCCGGATGCCATCGCCTGGCGGCTGGAACTGCCCGAGCCCCGCGC
CCGCAAGCTTACGGACTTCCGCGTGGCCGTGCTCTACGGCTCGTCCATCGCCGAGGTGGACGAGGCCTATGCCTCGCGTC
TCAGGGCGCTGGTGGAGCGCCTGCGCGCCGAGGGCGTGAATGTGAACGACAACGCCCGGCCGGATTTCGACGATGCCGAG
CACCATCATGTTCTGCTTCAGGTGCTGCGCGGGGCCGCGAGCGCCCGCCCGCCGCAGGCAGCCGTCGACGCGGCTCTGGC
ACGCCTGAAGACCACCGCCGCGGACGACCATTCCTATGTGGCCGAATCCGACCGGGCGCTGACCCAGAGCCACCGGGCGT
GGCTCGGCATTCAGGAGCGCCGGGCCGCCATCCAGAGGGCCTGGGCGTCCTTCTTCGAGAGCTATGACGTGCTGTTGGCG
CCGGCGACGACCGGGGCCGCCTTTCCGCTGGACGAGGACCGCCCCCGCGAGGCGCGCACCCTCACCATCAACGGCCGGCA
GGCGGATTATAACGACCAGCTCTTTTGGGCGGGCATTGCGACGCTGGCGGGGCTACCGGCGAGCGTCGCGCCCATCGGCG
AGACGGCGGACGGGCTGCCGGTTGGGGTCCAGATTGTCGGTCCCTATCTGGAGGACCGCACGCCCATCGCCTTCGCCGCC
GCGCTCGAAGCGCTCTACGGCTTCCGCCGCCCGCCCGGCCTCGATTGA

Upstream 100 bases:

>100_bases
TCGCCGCCATCGGCATGGCGGGATCGAGCCTCATGCGCTTCATCGCCAAGCGCATCGTCTTCTGGGAGGGCGGGCGCACC
GCGGCTCCGTCCGAGGAGGT

Downstream 100 bases:

>100_bases
GGCGGGGAGGGGCGACGAGCGCTCATCCGATCAGGCCTCGGGGAGGCCGAGGGTGGCGGCGAGTTCCCGCCACTCGCGGG
CGGAGAGGCCGCTGGTGGCC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSMTRSDTILPAPEALAFASAIDLLEALRRGDVTSRALLELYLGRIERHNPALNAVIFLEAEAARARADAADAARARGES
WGPLHGLPMTVKESHHIAGWPTTWGDPATADFRPEATGVVVQRLLDAGAIVFGKTNVPIHLLDWQSYNAIHGTTHNPWRR
GVTPGGSSGGSAVALAAGFTAAELGSDAGGSVRMPAHFCGVFGHRPSIHVVPQAGNDRPGSTIGNEVSTSGPMARSAGDL
DLLLGILAGPAGPDAIAWRLELPEPRARKLTDFRVAVLYGSSIAEVDEAYASRLRALVERLRAEGVNVNDNARPDFDDAE
HHHVLLQVLRGAASARPPQAAVDAALARLKTTAADDHSYVAESDRALTQSHRAWLGIQERRAAIQRAWASFFESYDVLLA
PATTGAAFPLDEDRPREARTLTINGRQADYNDQLFWAGIATLAGLPASVAPIGETADGLPVGVQIVGPYLEDRTPIAFAA
ALEALYGFRRPPGLD

Sequences:

>Translated_495_residues
MSMTRSDTILPAPEALAFASAIDLLEALRRGDVTSRALLELYLGRIERHNPALNAVIFLEAEAARARADAADAARARGES
WGPLHGLPMTVKESHHIAGWPTTWGDPATADFRPEATGVVVQRLLDAGAIVFGKTNVPIHLLDWQSYNAIHGTTHNPWRR
GVTPGGSSGGSAVALAAGFTAAELGSDAGGSVRMPAHFCGVFGHRPSIHVVPQAGNDRPGSTIGNEVSTSGPMARSAGDL
DLLLGILAGPAGPDAIAWRLELPEPRARKLTDFRVAVLYGSSIAEVDEAYASRLRALVERLRAEGVNVNDNARPDFDDAE
HHHVLLQVLRGAASARPPQAAVDAALARLKTTAADDHSYVAESDRALTQSHRAWLGIQERRAAIQRAWASFFESYDVLLA
PATTGAAFPLDEDRPREARTLTINGRQADYNDQLFWAGIATLAGLPASVAPIGETADGLPVGVQIVGPYLEDRTPIAFAA
ALEALYGFRRPPGLD
>Mature_494_residues
SMTRSDTILPAPEALAFASAIDLLEALRRGDVTSRALLELYLGRIERHNPALNAVIFLEAEAARARADAADAARARGESW
GPLHGLPMTVKESHHIAGWPTTWGDPATADFRPEATGVVVQRLLDAGAIVFGKTNVPIHLLDWQSYNAIHGTTHNPWRRG
VTPGGSSGGSAVALAAGFTAAELGSDAGGSVRMPAHFCGVFGHRPSIHVVPQAGNDRPGSTIGNEVSTSGPMARSAGDLD
LLLGILAGPAGPDAIAWRLELPEPRARKLTDFRVAVLYGSSIAEVDEAYASRLRALVERLRAEGVNVNDNARPDFDDAEH
HHVLLQVLRGAASARPPQAAVDAALARLKTTAADDHSYVAESDRALTQSHRAWLGIQERRAAIQRAWASFFESYDVLLAP
ATTGAAFPLDEDRPREARTLTINGRQADYNDQLFWAGIATLAGLPASVAPIGETADGLPVGVQIVGPYLEDRTPIAFAAA
LEALYGFRRPPGLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=253, Percent_Identity=35.5731225296443, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=25.8964143426295, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI166795287, Length=213, Percent_Identity=31.924882629108, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=508, Percent_Identity=27.3622047244095, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17556264, Length=184, Percent_Identity=36.9565217391304, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=216, Percent_Identity=32.4074074074074, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=220, Percent_Identity=33.1818181818182, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=470, Percent_Identity=26.1702127659574, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556278, Length=228, Percent_Identity=35.0877192982456, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=228, Percent_Identity=35.0877192982456, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17538254, Length=230, Percent_Identity=33.4782608695652, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=28.9256198347107, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=422, Percent_Identity=25.5924170616114, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=29.1338582677165, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648435, Length=243, Percent_Identity=35.8024691358025, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648437, Length=243, Percent_Identity=35.8024691358025, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550774, Length=243, Percent_Identity=35.8024691358025, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=28.2520325203252, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24652985, Length=253, Percent_Identity=35.5731225296443, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922090, Length=253, Percent_Identity=35.5731225296443, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652981, Length=253, Percent_Identity=35.5731225296443, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652983, Length=253, Percent_Identity=35.5731225296443, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078093, Length=521, Percent_Identity=27.831094049904, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648441, Length=177, Percent_Identity=38.9830508474576, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648439, Length=177, Percent_Identity=38.9830508474576, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648113, Length=474, Percent_Identity=28.0590717299578, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 52639; Mature: 52507

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTRSDTILPAPEALAFASAIDLLEALRRGDVTSRALLELYLGRIERHNPALNAVIFLE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
AEAARARADAADAARARGESWGPLHGLPMTVKESHHIAGWPTTWGDPATADFRPEATGVV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VQRLLDAGAIVFGKTNVPIHLLDWQSYNAIHGTTHNPWRRGVTPGGSSGGSAVALAAGFT
HHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEECCCH
AAELGSDAGGSVRMPAHFCGVFGHRPSIHVVPQAGNDRPGSTIGNEVSTSGPMARSAGDL
HHHCCCCCCCCEECCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCH
DLLLGILAGPAGPDAIAWRLELPEPRARKLTDFRVAVLYGSSIAEVDEAYASRLRALVER
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHH
LRAEGVNVNDNARPDFDDAEHHHVLLQVLRGAASARPPQAAVDAALARLKTTAADDHSYV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHH
AESDRALTQSHRAWLGIQERRAAIQRAWASFFESYDVLLAPATTGAAFPLDEDRPREART
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEE
LTINGRQADYNDQLFWAGIATLAGLPASVAPIGETADGLPVGVQIVGPYLEDRTPIAFAA
EEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCHHHHH
ALEALYGFRRPPGLD
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMTRSDTILPAPEALAFASAIDLLEALRRGDVTSRALLELYLGRIERHNPALNAVIFLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
AEAARARADAADAARARGESWGPLHGLPMTVKESHHIAGWPTTWGDPATADFRPEATGVV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VQRLLDAGAIVFGKTNVPIHLLDWQSYNAIHGTTHNPWRRGVTPGGSSGGSAVALAAGFT
HHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEECCCH
AAELGSDAGGSVRMPAHFCGVFGHRPSIHVVPQAGNDRPGSTIGNEVSTSGPMARSAGDL
HHHCCCCCCCCEECCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCH
DLLLGILAGPAGPDAIAWRLELPEPRARKLTDFRVAVLYGSSIAEVDEAYASRLRALVER
HHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHH
LRAEGVNVNDNARPDFDDAEHHHVLLQVLRGAASARPPQAAVDAALARLKTTAADDHSYV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHH
AESDRALTQSHRAWLGIQERRAAIQRAWASFFESYDVLLAPATTGAAFPLDEDRPREART
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEE
LTINGRQADYNDQLFWAGIATLAGLPASVAPIGETADGLPVGVQIVGPYLEDRTPIAFAA
EEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCHHHHH
ALEALYGFRRPPGLD
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA