Click here to switch to the map view.

The map label for this gene is ycdT [H]

Identifier: 158424140

GI number: 158424140

Start: 2853709

End: 2854806

Strand: Reverse

Name: ycdT [H]

Synonym: AZC_2516

Alternate gene names: 158424140

Gene position: 2854806-2853709 (Counterclockwise)

Preceding gene: 158424141

Following gene: 158424138

Centisome position: 53.16

GC content: 63.48

Gene sequence:

>1098_bases
ATGAAGAACATGCTCCCCTCTGGCCGAACGCGGACCAAACCCGAAATGAGCCCGGAATTCGCCAGACTGCGCACGCGTAT
CATGCGAATTCTGTTCGCTATCGGCTTTGTCTGCAATGTCTGGTTCTGGTTTTACGACAGCTATCAGGGCATCATCAGCG
CACGAAATGCCACCCTGACGGATCCGTTGCTGGTTCTCATCTGCGCGGTCGGCTTCATCGTCACGGATATCAGCGAGCGC
TGGCGGATCGTTGCGGAGCGAAGCCTGTTTCTCGCCTTCTGCATCGGCTGCGCGACCGGTGTCTTCACATTCGGCGATCC
GGACCCGCAGATCAGGCTCTATGTGATCGCCGACACGCTGCAATGGCTGCCGCTGATCAGCGTGGCGGCCTTCATCATCT
TTCCCGGCCGCGAGAGCATTCCCGCCGCGGTGATCGGCTTCCTCATCCCGGCGGCAACGGCGGTCTGGGTGTGGTTCAAC
AGTGAGCCGGCGAACTGGCCGGACACCACCAATGCGCTATTGCTGAACGCCATCATCGTGCAGGCCATTTCGCTGCTGGT
TCTCGTGCTCTTCTCCTACTCGCAGGCCGCCTACCAGCAGGCCAAGGCGCAGGCGAGCATGATGGAGAGCGCGGCTCTGT
CCGACCCGCTTACCGGCATCGCGAACCGGCGCGGCATCGAGCGGGCCCTTGAAATCCAGGGCGCCTCATCCCGGCAGTGG
GCGCTGATCCTGCTGGACATGGATTACTTCAAGACCGTCAATGACCAATGGGGGCACGTGTTCGGCGACCGCGTCCTTCA
GAACGTCGTCGATGCGCTTCGGCGCGTGCTGCGCGAAGGCGATGTCATCGGCCGTTGGGGCGGGGACGAGTTTCTGGTGG
TCTCGGCGGATCTGTCGGCGCCGGAGGCCGAAGCCCTCGCCGAGAAACTGCGTCAGGCGGTGGCCGTGTTGCCGGACGAC
GAGAGCGGTGGGGTCACCATTTCCGCCGGCGTCGCGCTCTGGGACGGGGATGGCGGTCTCGCCGAGGCTCTGCGGCTCGT
GGATGCGGCGCTCTATCGGTCGAAGCAGATGGGCCGGAACCGCGTCGTCTTTGCCTGA

Upstream 100 bases:

>100_bases
GGGTGCTGCGTCGAATGCGCCATTCGCGATAACGTGCGTATCTGGATGGAGCACCGCTTCCCAAGGCTGACAGGCTCACA
ACACAACGCGTCATTCGCGG

Downstream 100 bases:

>100_bases
GCGGCCTTGCCGAGGTCATCAGGTCAGCCCACGCCTGCTGCTGGACCGTCAGTTCAGGGCGGGATCATCCGGCTTCTCAG
GCTGCCGGGACGTGCTGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKNMLPSGRTRTKPEMSPEFARLRTRIMRILFAIGFVCNVWFWFYDSYQGIISARNATLTDPLLVLICAVGFIVTDISER
WRIVAERSLFLAFCIGCATGVFTFGDPDPQIRLYVIADTLQWLPLISVAAFIIFPGRESIPAAVIGFLIPAATAVWVWFN
SEPANWPDTTNALLLNAIIVQAISLLVLVLFSYSQAAYQQAKAQASMMESAALSDPLTGIANRRGIERALEIQGASSRQW
ALILLDMDYFKTVNDQWGHVFGDRVLQNVVDALRRVLREGDVIGRWGGDEFLVVSADLSAPEAEALAEKLRQAVAVLPDD
ESGGVTISAGVALWDGDGGLAEALRLVDAALYRSKQMGRNRVVFA

Sequences:

>Translated_365_residues
MKNMLPSGRTRTKPEMSPEFARLRTRIMRILFAIGFVCNVWFWFYDSYQGIISARNATLTDPLLVLICAVGFIVTDISER
WRIVAERSLFLAFCIGCATGVFTFGDPDPQIRLYVIADTLQWLPLISVAAFIIFPGRESIPAAVIGFLIPAATAVWVWFN
SEPANWPDTTNALLLNAIIVQAISLLVLVLFSYSQAAYQQAKAQASMMESAALSDPLTGIANRRGIERALEIQGASSRQW
ALILLDMDYFKTVNDQWGHVFGDRVLQNVVDALRRVLREGDVIGRWGGDEFLVVSADLSAPEAEALAEKLRQAVAVLPDD
ESGGVTISAGVALWDGDGGLAEALRLVDAALYRSKQMGRNRVVFA
>Mature_365_residues
MKNMLPSGRTRTKPEMSPEFARLRTRIMRILFAIGFVCNVWFWFYDSYQGIISARNATLTDPLLVLICAVGFIVTDISER
WRIVAERSLFLAFCIGCATGVFTFGDPDPQIRLYVIADTLQWLPLISVAAFIIFPGRESIPAAVIGFLIPAATAVWVWFN
SEPANWPDTTNALLLNAIIVQAISLLVLVLFSYSQAAYQQAKAQASMMESAALSDPLTGIANRRGIERALEIQGASSRQW
ALILLDMDYFKTVNDQWGHVFGDRVLQNVVDALRRVLREGDVIGRWGGDEFLVVSADLSAPEAEALAEKLRQAVAVLPDD
ESGGVTISAGVALWDGDGGLAEALRLVDAALYRSKQMGRNRVVFA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=38.6503067484663, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=37.6470588235294, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786584, Length=225, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=32.6923076923077, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=31.25, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=190, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=32.5581395348837, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787541, Length=153, Percent_Identity=33.9869281045752, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=33.5329341317365, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=32.9032258064516, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=190, Percent_Identity=32.1052631578947, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=32.3529411764706, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=30.4878048780488, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40206; Mature: 40206

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMLPSGRTRTKPEMSPEFARLRTRIMRILFAIGFVCNVWFWFYDSYQGIISARNATLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DPLLVLICAVGFIVTDISERWRIVAERSLFLAFCIGCATGVFTFGDPDPQIRLYVIADTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHH
QWLPLISVAAFIIFPGRESIPAAVIGFLIPAATAVWVWFNSEPANWPDTTNALLLNAIIV
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHH
QAISLLVLVLFSYSQAAYQQAKAQASMMESAALSDPLTGIANRRGIERALEIQGASSRQW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCE
ALILLDMDYFKTVNDQWGHVFGDRVLQNVVDALRRVLREGDVIGRWGGDEFLVVSADLSA
EEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCC
PEAEALAEKLRQAVAVLPDDESGGVTISAGVALWDGDGGLAEALRLVDAALYRSKQMGRN
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
RVVFA
CCCCC
>Mature Secondary Structure
MKNMLPSGRTRTKPEMSPEFARLRTRIMRILFAIGFVCNVWFWFYDSYQGIISARNATLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DPLLVLICAVGFIVTDISERWRIVAERSLFLAFCIGCATGVFTFGDPDPQIRLYVIADTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHH
QWLPLISVAAFIIFPGRESIPAAVIGFLIPAATAVWVWFNSEPANWPDTTNALLLNAIIV
HHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHH
QAISLLVLVLFSYSQAAYQQAKAQASMMESAALSDPLTGIANRRGIERALEIQGASSRQW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCE
ALILLDMDYFKTVNDQWGHVFGDRVLQNVVDALRRVLREGDVIGRWGGDEFLVVSADLSA
EEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCC
PEAEALAEKLRQAVAVLPDDESGGVTISAGVALWDGDGGLAEALRLVDAALYRSKQMGRN
CHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
RVVFA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]