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The map label for this gene is gyrB [H]

Identifier: 158424137

GI number: 158424137

Start: 2848440

End: 2850458

Strand: Direct

Name: gyrB [H]

Synonym: AZC_2513

Alternate gene names: 158424137

Gene position: 2848440-2850458 (Clockwise)

Preceding gene: 158424136

Following gene: 158424139

Centisome position: 53.05

GC content: 67.31

Gene sequence:

>2019_bases
ATGTCCAATTCCGACGACCTCTTCTCCAATCCCCCTCCCTCCCGCGGCCGCAAGACCACGGTCGTGCCGCAGATGGCGAG
CGACTATACCGCCGCCCATATCGAGGTGCTGGAAGGGCTGGAGCCGGTGCGGCGCCGGCCCGGCATGTATATCGGCGGCA
CCGATGCCAAGGCGCTGCATCACCTCTTCGCGGAGGTGATCGACAACTCGATGGACGAGGCGGTGGCGGGCCATGCCACT
TTCATCGAGGTGCAGCTCTCCAATGACGGCTCGCTGACGGTGATCGACAACGGGCGCGGCATCCCGGTGGAGGAACACCC
CAAGTTCCCCGGCAAGTCGGCGCTCGAAGTCATCATGACCACGCTGCACTCGGGCGGAAAGTTCGACAGCAAGGTCTATG
AGACCTCCGGCGGCCTGCACGGCGTCGGCGTCTCGGTGGTGAACGCGCTCTCCGATGCCTTCGAGGTGGAGGTCGCCCGC
AATCAGACGCTCTACCGCCAGACCTTCTCGCGCGGCACGCCGCAGGGCAAGCTGAAGGAAGTGGGCCGCATCACCAACCG
GCGCGGCACCAAGATCCGCTTCCATCCCGATCCGGACATCTTTGGCCCGAGCGCCAAGTTCGAGCCCGGCCGCGTGTTCC
GCATGGCGCGCGCCAAGGCCTATCTGTTCGGTGGCGTGGAAATTCGCTGGTCCTGCGATCCCGAACTGCTCGCCGGCACC
ACCGGCGTGCCGGAAAAGATGACCTTCCGCTTCCCCGGCGGCCTCAGGGACTATCTGGCCGCCGACATCGAGGGCCAGAC
CCTCGTGCATGGCGACATCTTCTCGGGCCGCGTGCAGAAGCCGGGCGGCCATGGCTCGGCCGAATGGGCGGTGGCCTGGA
TCGCCGATGGCGACGGCGGCATCTCCTCTTACTGCAACACCATCCCGACGCCGGACGGCGGGACCCATGAGAACGGCCTG
CGCGCCGTGCTGCTCAAGGGCCTGCGCGAGCATGGCGAGCGGACCAACCAGTCCAAGCGCGCCGCCCTCATCACCGGCGA
GGACGTAACCGCCGGCTGCGCGGCCATGCTCTCCGTCTTTGTGCGCGAGCCGGAATTCCAGGGCCAGACCAAGGAGCGTC
TCGCCACTGCCGAGGCGGCCCGCATCGTCGAGCAGACGGTGCGCGATCCCTTCGACCACTGGCTCGCCGCCAATCCGGCC
CAGGCCACGCGCCTGTTCGACCATGTGGTGGAGCGGGCGGAGGAGCGGCTGCGGCGCAAGCAGGAGAAGGAAATCTCCCG
CAAGACCGCCGTGCGCAAGCTGCGCCTGCCCGGCAAGCTGGCGGACTGCTCCAACACCGAGGCGGCCGGCTCCGAACTCT
TCATCGTCGAGGGCGACTCGGCCGGCGGCTCCGCCAAGCAGGCCCGCGACCGCCGCACCCAGGCGGTGCTGCCCCTGCGC
GGCAAGATCCTCAACGTCGCCAATGCCGGCCGCGACAAGCTTGCCCAGAACCAGCAATTGGCCGATCTCGGCCAGGCGCT
CGGCTGCGGCACGCTCTCGCACTATCGCGAGGCGGACCTGCGCTATGAGAAGGTCATCATCATGACCGACGCGGACGTGG
ACGGCGCCCACATCGCCTCCCTCCTCGTCACCTTCTTCTATCGCCAGATGCCGAAGCTGGTGGACGAGGGACATCTCTTC
CTCGCCGTGCCGCCGCTCTATCGCATCACGCAGGGCGCCAAGACGCTCTATGCCCGCGACGACGCCCACAAGGAGAAGCT
CCTGAAGAAGGAGTTCAACGGGCGCGGCAAGGTGGAGATCGGCCGCTTCAAAGGCCTCGGCGAGATGATGCCGGCGCAGC
TCAAGGAAACCACAATGGACCCGAAGACGCGCACGCTGCTTCAGGTCAGCATCGAGGAGGCGGCCCGCGCCGCCACCGAC
GACATCGTGGAACGCCTCATGGGCAACAAGCCGGAGGCGCGCTTCGCCTTCATCTCCGAGCACGCCGCCTTCGCGGGCGA
GGAACTGCTCGACATCTGA

Upstream 100 bases:

>100_bases
GCCGAGGCGACTCGGCTAGAAAGTTCGGCTACAAGGGCACAAACCGTGTACATCCCGCGTCCGCCGCAAGGCCGGGCGCC
TCGCGAGATCCGATCGCTAC

Downstream 100 bases:

>100_bases
GCCCCGGAGCGCGGTCCCGAGAGTCTGAGGCGCCTGTCGCGGCTCAGACCGGCTCCACCGACTGGACCTTGCGGGCGGCG
GTCTCCTCCGCCTCCCGGCG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MSNSDDLFSNPPPSRGRKTTVVPQMASDYTAAHIEVLEGLEPVRRRPGMYIGGTDAKALHHLFAEVIDNSMDEAVAGHAT
FIEVQLSNDGSLTVIDNGRGIPVEEHPKFPGKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGVSVVNALSDAFEVEVAR
NQTLYRQTFSRGTPQGKLKEVGRITNRRGTKIRFHPDPDIFGPSAKFEPGRVFRMARAKAYLFGGVEIRWSCDPELLAGT
TGVPEKMTFRFPGGLRDYLAADIEGQTLVHGDIFSGRVQKPGGHGSAEWAVAWIADGDGGISSYCNTIPTPDGGTHENGL
RAVLLKGLREHGERTNQSKRAALITGEDVTAGCAAMLSVFVREPEFQGQTKERLATAEAARIVEQTVRDPFDHWLAANPA
QATRLFDHVVERAEERLRRKQEKEISRKTAVRKLRLPGKLADCSNTEAAGSELFIVEGDSAGGSAKQARDRRTQAVLPLR
GKILNVANAGRDKLAQNQQLADLGQALGCGTLSHYREADLRYEKVIIMTDADVDGAHIASLLVTFFYRQMPKLVDEGHLF
LAVPPLYRITQGAKTLYARDDAHKEKLLKKEFNGRGKVEIGRFKGLGEMMPAQLKETTMDPKTRTLLQVSIEEAARAATD
DIVERLMGNKPEARFAFISEHAAFAGEELLDI

Sequences:

>Translated_672_residues
MSNSDDLFSNPPPSRGRKTTVVPQMASDYTAAHIEVLEGLEPVRRRPGMYIGGTDAKALHHLFAEVIDNSMDEAVAGHAT
FIEVQLSNDGSLTVIDNGRGIPVEEHPKFPGKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGVSVVNALSDAFEVEVAR
NQTLYRQTFSRGTPQGKLKEVGRITNRRGTKIRFHPDPDIFGPSAKFEPGRVFRMARAKAYLFGGVEIRWSCDPELLAGT
TGVPEKMTFRFPGGLRDYLAADIEGQTLVHGDIFSGRVQKPGGHGSAEWAVAWIADGDGGISSYCNTIPTPDGGTHENGL
RAVLLKGLREHGERTNQSKRAALITGEDVTAGCAAMLSVFVREPEFQGQTKERLATAEAARIVEQTVRDPFDHWLAANPA
QATRLFDHVVERAEERLRRKQEKEISRKTAVRKLRLPGKLADCSNTEAAGSELFIVEGDSAGGSAKQARDRRTQAVLPLR
GKILNVANAGRDKLAQNQQLADLGQALGCGTLSHYREADLRYEKVIIMTDADVDGAHIASLLVTFFYRQMPKLVDEGHLF
LAVPPLYRITQGAKTLYARDDAHKEKLLKKEFNGRGKVEIGRFKGLGEMMPAQLKETTMDPKTRTLLQVSIEEAARAATD
DIVERLMGNKPEARFAFISEHAAFAGEELLDI
>Mature_671_residues
SNSDDLFSNPPPSRGRKTTVVPQMASDYTAAHIEVLEGLEPVRRRPGMYIGGTDAKALHHLFAEVIDNSMDEAVAGHATF
IEVQLSNDGSLTVIDNGRGIPVEEHPKFPGKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGVSVVNALSDAFEVEVARN
QTLYRQTFSRGTPQGKLKEVGRITNRRGTKIRFHPDPDIFGPSAKFEPGRVFRMARAKAYLFGGVEIRWSCDPELLAGTT
GVPEKMTFRFPGGLRDYLAADIEGQTLVHGDIFSGRVQKPGGHGSAEWAVAWIADGDGGISSYCNTIPTPDGGTHENGLR
AVLLKGLREHGERTNQSKRAALITGEDVTAGCAAMLSVFVREPEFQGQTKERLATAEAARIVEQTVRDPFDHWLAANPAQ
ATRLFDHVVERAEERLRRKQEKEISRKTAVRKLRLPGKLADCSNTEAAGSELFIVEGDSAGGSAKQARDRRTQAVLPLRG
KILNVANAGRDKLAQNQQLADLGQALGCGTLSHYREADLRYEKVIIMTDADVDGAHIASLLVTFFYRQMPKLVDEGHLFL
AVPPLYRITQGAKTLYARDDAHKEKLLKKEFNGRGKVEIGRFKGLGEMMPAQLKETTMDPKTRTLLQVSIEEAARAATDD
IVERLMGNKPEARFAFISEHAAFAGEELLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=635, Percent_Identity=24.5669291338583, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI19913406, Length=629, Percent_Identity=23.5294117647059, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI1789408, Length=648, Percent_Identity=43.3641975308642, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=43.3155080213904, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI212645845, Length=575, Percent_Identity=25.3913043478261, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17535065, Length=585, Percent_Identity=23.4188034188034, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324241, Length=640, Percent_Identity=23.75, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=637, Percent_Identity=26.0596546310832, Blast_Score=125, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73557; Mature: 73426

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSDDLFSNPPPSRGRKTTVVPQMASDYTAAHIEVLEGLEPVRRRPGMYIGGTDAKALH
CCCCCCCCCCCCCCCCCCEEECCCHHCCHHHHHHHHHHCCHHHHHCCCEEECCCCHHHHH
HLFAEVIDNSMDEAVAGHATFIEVQLSNDGSLTVIDNGRGIPVEEHPKFPGKSALEVIMT
HHHHHHHCCHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
TLHSGGKFDSKVYETSGGLHGVGVSVVNALSDAFEVEVARNQTLYRQTFSRGTPQGKLKE
HHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEHHHHCHHHHHHHHHCCCCCHHHHH
VGRITNRRGTKIRFHPDPDIFGPSAKFEPGRVFRMARAKAYLFGGVEIRWSCDPELLAGT
HHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEECEEEEECCCHHHHCCC
TGVPEKMTFRFPGGLRDYLAADIEGQTLVHGDIFSGRVQKPGGHGSAEWAVAWIADGDGG
CCCCCCEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ISSYCNTIPTPDGGTHENGLRAVLLKGLREHGERTNQSKRAALITGEDVTAGCAAMLSVF
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
VREPEFQGQTKERLATAEAARIVEQTVRDPFDHWLAANPAQATRLFDHVVERAEERLRRK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QEKEISRKTAVRKLRLPGKLADCSNTEAAGSELFIVEGDSAGGSAKQARDRRTQAVLPLR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCC
GKILNVANAGRDKLAQNQQLADLGQALGCGTLSHYREADLRYEKVIIMTDADVDGAHIAS
CCEEEHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
LLVTFFYRQMPKLVDEGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFNGRGKVEI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCHHEEECCHHHHHHHHHHHCCCCCCEEE
GRFKGLGEMMPAQLKETTMDPKTRTLLQVSIEEAARAATDDIVERLMGNKPEARFAFISE
HHHCCCHHHHHHHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
HAAFAGEELLDI
HHHHCCHHHHCC
>Mature Secondary Structure 
SNSDDLFSNPPPSRGRKTTVVPQMASDYTAAHIEVLEGLEPVRRRPGMYIGGTDAKALH
CCCCCCCCCCCCCCCCCEEECCCHHCCHHHHHHHHHHCCHHHHHCCCEEECCCCHHHHH
HLFAEVIDNSMDEAVAGHATFIEVQLSNDGSLTVIDNGRGIPVEEHPKFPGKSALEVIMT
HHHHHHHCCHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
TLHSGGKFDSKVYETSGGLHGVGVSVVNALSDAFEVEVARNQTLYRQTFSRGTPQGKLKE
HHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHEEHHHHCHHHHHHHHHCCCCCHHHHH
VGRITNRRGTKIRFHPDPDIFGPSAKFEPGRVFRMARAKAYLFGGVEIRWSCDPELLAGT
HHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEECEEEEECCCHHHHCCC
TGVPEKMTFRFPGGLRDYLAADIEGQTLVHGDIFSGRVQKPGGHGSAEWAVAWIADGDGG
CCCCCCEEEECCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ISSYCNTIPTPDGGTHENGLRAVLLKGLREHGERTNQSKRAALITGEDVTAGCAAMLSVF
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
VREPEFQGQTKERLATAEAARIVEQTVRDPFDHWLAANPAQATRLFDHVVERAEERLRRK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QEKEISRKTAVRKLRLPGKLADCSNTEAAGSELFIVEGDSAGGSAKQARDRRTQAVLPLR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCC
GKILNVANAGRDKLAQNQQLADLGQALGCGTLSHYREADLRYEKVIIMTDADVDGAHIAS
CCEEEHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHH
LLVTFFYRQMPKLVDEGHLFLAVPPLYRITQGAKTLYARDDAHKEKLLKKEFNGRGKVEI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCHHEEECCHHHHHHHHHHHCCCCCCEEE
GRFKGLGEMMPAQLKETTMDPKTRTLLQVSIEEAARAATDDIVERLMGNKPEARFAFISE
HHHCCCHHHHHHHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
HAAFAGEELLDI
HHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]