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The map label for this gene is potH [H]

Identifier: 158424101

GI number: 158424101

Start: 2814018

End: 2815790

Strand: Direct

Name: potH [H]

Synonym: AZC_2477

Alternate gene names: 158424101

Gene position: 2814018-2815790 (Clockwise)

Preceding gene: 158424100

Following gene: 158424102

Centisome position: 52.4

GC content: 66.78

Gene sequence:

>1773_bases
ATGAGCACCGCACTCTCCGCTCCCGCCCGCGCCGCGGCCCGCCGGAAGGCGTTCGGCCGCGCGCTCATGGCGGCGCCGCT
CATCGTGCTGCTGCTGGTCTTCCTGGTCTATCCGGTGGGGCAGTTACTGCTGCTCAGCGTCTGGGGCGCCAAGGGCTTCA
GCCTTCAGGCCTACGCGCCGCTGTTCTCCTCGCCCCTCTATATCAACGTTCTGCTCATCACGTTGAAGATCTCCGCCATC
ACCACGCTCCTGTCGGTGATCGGCGGCTATCCGATCGCCTATCTCATTTCCGTCTCCGCCCCCGAGCGCAAGGCGCGGCT
GCTGTTCTGGGTGCTGCTGTCCTTCTGGACCAGCTTCCTCGTACGCGCCTTCGCCTGGATCGTCATCCTCGGCCGCCGGG
GCGTCGTGAACCATCTGCTGCTGAGCCTCGGCCTCGCCGACCAGCCCGCCGACCTCCTCTATAATTTCAGCAGCGTCATC
ATCGGCATGGTGCATGCGCTGATGCCGCTCGCCATCCTCACCATGCTCTCGGTGATGGAGAACATCGACCGCACCCTGCC
CCGCGCCGCCTCGACGCTGGGCGCGCGGCCCGGCACGGCCTTCTGGAAAATCTATTTCCCGCTCTCTCTGCCGGGCGTTG
CCGCGGCGGCCATCATGGTGTTCGTCACCGCCATCGGCTTCTTCATCGTGCCCGCCCTCCTCGGCGGACGGCGTGAGACG
ATGATCACGCAGCTCATCATCGACCAGGTGCTCCAGACCATGAACTGGGGCTTCGCCGGCGCCATCTCGGTGCTGCTGCT
GGTGGTCGTGCTGGTGGTCTTCCTCGCCTATGACAAGCTCGTCGGCCTCTCGACCATGACCGGCTCGGGCAGCACGCGCC
CGGCCAAGGCCACCTCCGGCGGTCTTTCGCAGCGGATCGGCGATGCCCTGCTCACCGCCCTCGGCACGGCCAGCGACGCG
CTGATCTCGCTCTTTCCCCACCGCAAGACGGCGAGCGGAAAGGCACCCCTCCCCCTCGTCTTCTTCGTCGTCGCCCTGCT
CGTGCTCATGAGCCTTCCGGCCGTGCTCATGATCCCGCTGTCCTTCGGCTCGGGCGGACTCAACTGGCCGCCGCGCGGCT
TCACGCTGGAATGGTATGAGCAGATGCTCCAGTCGCCCGTCTGGGGGCAGGCGCTCATGCGCTCGCTGCTGGTGGGCCTC
GGCACCGGCTTCCTTGCCATGCTCATCGGCACGCCCGCCGCCTTCCTCCTGGTGCGCAGCGACATGAAGGGCAAGGGCGC
GATCCTCGCCTTCGTGCTGTCGCCCATCGTCGTGCCGCGCATGATCATCGCTGTGGGCCTGTTCTACATGTTCGCCCGCA
TCGGCCTCGTCGGCACGGCCCTCGGCCTCATCCTCGGCCACACGGTCGTCGCCGTGCCCTATGTGGTGATGACCATGATG
GCGGTGCTGCGGAACTATGACACCCGCCTCGATCTCGCGGCCCAGAGCCTGGGCGCGCGCCCGCTCGCAACGCTGCGCTA
TGTGACATTGCCGATCCTCGGGGCGGGCATGCTGTCGTCCTTCCTGTTCGCCTTCGCGACCTCCTTCGACGAGCTGACGA
TCGCGCTCTTTGCCTCCGGCGGCCTCAACGCCACCTTGCCCAAGCAGTTCTGGGACGAGGTGACGCTCCAGGTGTCCCCC
ATGATCGCAGCGGTTTCGACCTGCCTCTTCATCTTCATCGCGGTGCTGATCTTCGTTGCCGACCGGCTGCGCCGGCGCAG
CCTCGCCAGCTGA

Upstream 100 bases:

>100_bases
TATCCGACCCGCACCCTCAGCGACGACCACAGCATCGGCGACGTGCTCGAACTGGACTGGCAGGCGCAGGATGCGGTCGC
CATTCCAGCGGGACAGTGAC

Downstream 100 bases:

>100_bases
CCGCAAACCCCGTCACAGACCCAGGGCTCTCCATGCTGACCTCTTCCCAACTGCGCGGCATCTTTCCGGCCACTCCGACG
CCGGTGAACGCGGACGACAC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MSTALSAPARAAARRKAFGRALMAAPLIVLLLVFLVYPVGQLLLLSVWGAKGFSLQAYAPLFSSPLYINVLLITLKISAI
TTLLSVIGGYPIAYLISVSAPERKARLLFWVLLSFWTSFLVRAFAWIVILGRRGVVNHLLLSLGLADQPADLLYNFSSVI
IGMVHALMPLAILTMLSVMENIDRTLPRAASTLGARPGTAFWKIYFPLSLPGVAAAAIMVFVTAIGFFIVPALLGGRRET
MITQLIIDQVLQTMNWGFAGAISVLLLVVVLVVFLAYDKLVGLSTMTGSGSTRPAKATSGGLSQRIGDALLTALGTASDA
LISLFPHRKTASGKAPLPLVFFVVALLVLMSLPAVLMIPLSFGSGGLNWPPRGFTLEWYEQMLQSPVWGQALMRSLLVGL
GTGFLAMLIGTPAAFLLVRSDMKGKGAILAFVLSPIVVPRMIIAVGLFYMFARIGLVGTALGLILGHTVVAVPYVVMTMM
AVLRNYDTRLDLAAQSLGARPLATLRYVTLPILGAGMLSSFLFAFATSFDELTIALFASGGLNATLPKQFWDEVTLQVSP
MIAAVSTCLFIFIAVLIFVADRLRRRSLAS

Sequences:

>Translated_590_residues
MSTALSAPARAAARRKAFGRALMAAPLIVLLLVFLVYPVGQLLLLSVWGAKGFSLQAYAPLFSSPLYINVLLITLKISAI
TTLLSVIGGYPIAYLISVSAPERKARLLFWVLLSFWTSFLVRAFAWIVILGRRGVVNHLLLSLGLADQPADLLYNFSSVI
IGMVHALMPLAILTMLSVMENIDRTLPRAASTLGARPGTAFWKIYFPLSLPGVAAAAIMVFVTAIGFFIVPALLGGRRET
MITQLIIDQVLQTMNWGFAGAISVLLLVVVLVVFLAYDKLVGLSTMTGSGSTRPAKATSGGLSQRIGDALLTALGTASDA
LISLFPHRKTASGKAPLPLVFFVVALLVLMSLPAVLMIPLSFGSGGLNWPPRGFTLEWYEQMLQSPVWGQALMRSLLVGL
GTGFLAMLIGTPAAFLLVRSDMKGKGAILAFVLSPIVVPRMIIAVGLFYMFARIGLVGTALGLILGHTVVAVPYVVMTMM
AVLRNYDTRLDLAAQSLGARPLATLRYVTLPILGAGMLSSFLFAFATSFDELTIALFASGGLNATLPKQFWDEVTLQVSP
MIAAVSTCLFIFIAVLIFVADRLRRRSLAS
>Mature_589_residues
STALSAPARAAARRKAFGRALMAAPLIVLLLVFLVYPVGQLLLLSVWGAKGFSLQAYAPLFSSPLYINVLLITLKISAIT
TLLSVIGGYPIAYLISVSAPERKARLLFWVLLSFWTSFLVRAFAWIVILGRRGVVNHLLLSLGLADQPADLLYNFSSVII
GMVHALMPLAILTMLSVMENIDRTLPRAASTLGARPGTAFWKIYFPLSLPGVAAAAIMVFVTAIGFFIVPALLGGRRETM
ITQLIIDQVLQTMNWGFAGAISVLLLVVVLVVFLAYDKLVGLSTMTGSGSTRPAKATSGGLSQRIGDALLTALGTASDAL
ISLFPHRKTASGKAPLPLVFFVVALLVLMSLPAVLMIPLSFGSGGLNWPPRGFTLEWYEQMLQSPVWGQALMRSLLVGLG
TGFLAMLIGTPAAFLLVRSDMKGKGAILAFVLSPIVVPRMIIAVGLFYMFARIGLVGTALGLILGHTVVAVPYVVMTMMA
VLRNYDTRLDLAAQSLGARPLATLRYVTLPILGAGMLSSFLFAFATSFDELTIALFASGGLNATLPKQFWDEVTLQVSPM
IAAVSTCLFIFIAVLIFVADRLRRRSLAS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=35.8407079646018, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1787369, Length=225, Percent_Identity=35.1111111111111, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1787368, Length=227, Percent_Identity=27.7533039647577, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=29.4372294372294, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787081, Length=261, Percent_Identity=28.735632183908, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787714, Length=218, Percent_Identity=31.651376146789, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 63226; Mature: 63095

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTALSAPARAAARRKAFGRALMAAPLIVLLLVFLVYPVGQLLLLSVWGAKGFSLQAYAP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCH
LFSSPLYINVLLITLKISAITTLLSVIGGYPIAYLISVSAPERKARLLFWVLLSFWTSFL
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHH
VRAFAWIVILGRRGVVNHLLLSLGLADQPADLLYNFSSVIIGMVHALMPLAILTMLSVME
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIDRTLPRAASTLGARPGTAFWKIYFPLSLPGVAAAAIMVFVTAIGFFIVPALLGGRRET
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MITQLIIDQVLQTMNWGFAGAISVLLLVVVLVVFLAYDKLVGLSTMTGSGSTRPAKATSG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCC
GLSQRIGDALLTALGTASDALISLFPHRKTASGKAPLPLVFFVVALLVLMSLPAVLMIPL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHEEEE
SFGSGGLNWPPRGFTLEWYEQMLQSPVWGQALMRSLLVGLGTGFLAMLIGTPAAFLLVRS
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
DMKGKGAILAFVLSPIVVPRMIIAVGLFYMFARIGLVGTALGLILGHTVVAVPYVVMTMM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRNYDTRLDLAAQSLGARPLATLRYVTLPILGAGMLSSFLFAFATSFDELTIALFASG
HHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECC
GLNATLPKQFWDEVTLQVSPMIAAVSTCLFIFIAVLIFVADRLRRRSLAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STALSAPARAAARRKAFGRALMAAPLIVLLLVFLVYPVGQLLLLSVWGAKGFSLQAYAP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCH
LFSSPLYINVLLITLKISAITTLLSVIGGYPIAYLISVSAPERKARLLFWVLLSFWTSFL
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHH
VRAFAWIVILGRRGVVNHLLLSLGLADQPADLLYNFSSVIIGMVHALMPLAILTMLSVME
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIDRTLPRAASTLGARPGTAFWKIYFPLSLPGVAAAAIMVFVTAIGFFIVPALLGGRRET
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
MITQLIIDQVLQTMNWGFAGAISVLLLVVVLVVFLAYDKLVGLSTMTGSGSTRPAKATSG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCC
GLSQRIGDALLTALGTASDALISLFPHRKTASGKAPLPLVFFVVALLVLMSLPAVLMIPL
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHEEEE
SFGSGGLNWPPRGFTLEWYEQMLQSPVWGQALMRSLLVGLGTGFLAMLIGTPAAFLLVRS
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
DMKGKGAILAFVLSPIVVPRMIIAVGLFYMFARIGLVGTALGLILGHTVVAVPYVVMTMM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRNYDTRLDLAAQSLGARPLATLRYVTLPILGAGMLSSFLFAFATSFDELTIALFASG
HHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECC
GLNATLPKQFWDEVTLQVSPMIAAVSTCLFIFIAVLIFVADRLRRRSLAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]