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The map label for this gene is gph [H]

Identifier: 158423983

GI number: 158423983

Start: 2687688

End: 2688374

Strand: Direct

Name: gph [H]

Synonym: AZC_2359

Alternate gene names: 158423983

Gene position: 2687688-2688374 (Clockwise)

Preceding gene: 158423980

Following gene: 158423984

Centisome position: 50.05

GC content: 67.54

Gene sequence:

>687_bases
ATGTCTCAGGTTCGCCCGGTGATCGCCTTCGACCTCGACGGGACACTCGTCGATACGGCACCGGACCTTCTCTCAACCCT
CGACGTGGTGCTCTCCGCCCATGGCATCGCGCCGGTGGACCGCGCGGAAGCACGCCCCATGATCGGCGGCGGCGCCCGGA
TGCTCATCGTGCGGGCGTTGAAGCGCGCCGGCGTCGACGTTCCACCGGAGGAACTCGACCGCATGAACCGGCAATTCCTC
GACCATTACGCCGCTCACATCGCAGATGAGTCGCGCCCCTTCCCCGGCATGGTGGAGGCGCTCGACAGGCTTGCCGCCCA
GGGCGCCGTCCTCGCGGTCTGCACCAACAAGCTCGAATATCTCGCCCGCCTGCTGCTGGACGGCCTCGGCCTCTCGCCAC
GCTTTGCCGCCATTACCGGCGCGGACACCTATGGCATCGCCAAGCCCGACCCGCTGCCGCTCCTCTCCACCATTTCCGCC
TGCGGCGGCACGCCCGCCCGTGCGGTGATGGTGGGCGACAGCGCCACTGACATCGCCACGGCCAAGGCAGCGCAGATTCC
CTCGGTGGCGGTCTCCTTCGGCTATACGGAGACGCCGGCCGCCCATCTCGGCGCCGATCGGCTCATCGAGCATTACGCTG
ACTTGGTTCGGGCTGTGGAAGACGTACTGTCATCCACAGCCGCCTAA

Upstream 100 bases:

>100_bases
CCGCAAACGCCGCCCTTGAAGGTGCGGCCGAACCTGCGACAAGGAGGCAAGGCGCCCCGTGCGCCGCGTTTGCTCCGATG
ACTTCAGCAGGGATTGCGTC

Downstream 100 bases:

>100_bases
GGGTGTCAGCGGGGGCGAGACGGGGGGACGCTTGACACCCCCGAGCCCACGACCTAAACAGCCGACCTCGCCGGTTGGCA
ACTGCCCCGGCGCCGCATCG

Product: 2-phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSQVRPVIAFDLDGTLVDTAPDLLSTLDVVLSAHGIAPVDRAEARPMIGGGARMLIVRALKRAGVDVPPEELDRMNRQFL
DHYAAHIADESRPFPGMVEALDRLAAQGAVLAVCTNKLEYLARLLLDGLGLSPRFAAITGADTYGIAKPDPLPLLSTISA
CGGTPARAVMVGDSATDIATAKAAQIPSVAVSFGYTETPAAHLGADRLIEHYADLVRAVEDVLSSTAA

Sequences:

>Translated_228_residues
MSQVRPVIAFDLDGTLVDTAPDLLSTLDVVLSAHGIAPVDRAEARPMIGGGARMLIVRALKRAGVDVPPEELDRMNRQFL
DHYAAHIADESRPFPGMVEALDRLAAQGAVLAVCTNKLEYLARLLLDGLGLSPRFAAITGADTYGIAKPDPLPLLSTISA
CGGTPARAVMVGDSATDIATAKAAQIPSVAVSFGYTETPAAHLGADRLIEHYADLVRAVEDVLSSTAA
>Mature_227_residues
SQVRPVIAFDLDGTLVDTAPDLLSTLDVVLSAHGIAPVDRAEARPMIGGGARMLIVRALKRAGVDVPPEELDRMNRQFLD
HYAAHIADESRPFPGMVEALDRLAAQGAVLAVCTNKLEYLARLLLDGLGLSPRFAAITGADTYGIAKPDPLPLLSTISAC
GGTPARAVMVGDSATDIATAKAAQIPSVAVSFGYTETPAAHLGADRLIEHYADLVRAVEDVLSSTAA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=230, Percent_Identity=34.7826086956522, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23865; Mature: 23733

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVRPVIAFDLDGTLVDTAPDLLSTLDVVLSAHGIAPVDRAEARPMIGGGARMLIVRAL
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHH
KRAGVDVPPEELDRMNRQFLDHYAAHIADESRPFPGMVEALDRLAAQGAVLAVCTNKLEY
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LARLLLDGLGLSPRFAAITGADTYGIAKPDPLPLLSTISACGGTPARAVMVGDSATDIAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHH
AKAAQIPSVAVSFGYTETPAAHLGADRLIEHYADLVRAVEDVLSSTAA
HHHHHCCHHHEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQVRPVIAFDLDGTLVDTAPDLLSTLDVVLSAHGIAPVDRAEARPMIGGGARMLIVRAL
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHH
KRAGVDVPPEELDRMNRQFLDHYAAHIADESRPFPGMVEALDRLAAQGAVLAVCTNKLEY
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LARLLLDGLGLSPRFAAITGADTYGIAKPDPLPLLSTISACGGTPARAVMVGDSATDIAT
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHH
AKAAQIPSVAVSFGYTETPAAHLGADRLIEHYADLVRAVEDVLSSTAA
HHHHHCCHHHEECCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]