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The map label for this gene is folD

Identifier: 158423930

GI number: 158423930

Start: 2630612

End: 2631484

Strand: Reverse

Name: folD

Synonym: AZC_2306

Alternate gene names: 158423930

Gene position: 2631484-2630612 (Counterclockwise)

Preceding gene: 158423931

Following gene: 158423929

Centisome position: 49.01

GC content: 68.38

Gene sequence:

>873_bases
GTGATCGAGACCAAGCCCATCGACGGAAAGGCGTTCGCCGCTGGCCTGCGCGCCCGCATTGCCGAAGAGGTCGCCGTACT
GGTGCGGGACCATGACCTGAAGCCCGGTCTCGCGGTGGTGCTGGTGGGCGAGGACCCGGCGAGCCAGGTCTATGTGCGCA
ACAAGGCCGCCCAGACGGCGGAGGCCGGCATGGCCTCATTCGAGTACAAGCTGCCGGCCGACACGGCGGAAGCCGACCTT
CTGGCGCTGGTGGAAAAGCTCAACGCCGATCCGGCGGTGAACGGCATTCTCGTGCAGCTTCCGCTGCCGGCCCATCTCGA
TTCCATGAAGGTCCTGGCCGCCATCGATCCGGCAAAGGATGTGGACGGCTTCCATGTGGTGAATGCCGGCCGCCTCGCGG
TGGGCCTCGACGCCCTCGTGCCCTGCACGCCGCTCGGCTGCGTGATGCTGCTGAAGCATCACCTGGGCAATCTCTCCGGG
CTCAATGCCGTGGTGGTTGGGCGCTCCAACATCGTCGGCAAGCCGGCGGCGCAGCTTCTGCTGCGGGAGGACTGCACCGT
TACGATCGCCCATTCGCGCACCCGCGATCTGCCGGGCATGTGCCGCCAGGCTGACATTCTGGTTGCCGCCGTCGGCCGCC
CGGAGATGGTGCGGGGCGACTGGATCAAGCCCGGTGCCACGGTCATCGACGTCGGCATCAACCGCGTGCCGAAGGCGGAC
GGCAAGACGCGCCTTGTGGGCGATGTGGCCTATGAGGAGGCGCTGGGCGTCGCCGGCCTCATCACGCCCGTGCCCGGCGG
CGTCGGCCCCATGACCATCGCCTGCCTTCTCCAGAACACCCTCACCGCCGCCCGCCGCCAGAAGGGGCTCTGA

Upstream 100 bases:

>100_bases
CGTTGCCTGAGCGCAGGGGAGGGGCGCGCCTTTTGGGGTTGCACCGTTCCGCCTTGCCGCCCAGAACACGCTCGCCTTCA
CAAGCCCCGAGGATCGCTCC

Downstream 100 bases:

>100_bases
GTCCGCGCGTGGCCCCCGGCAGGGCGCGCATGTCGCAGGGCCGCGCCGCCAAGGCGCGGCCCTCTGCCACTTGGAAGAGA
TCCAAACCGGGCTATATCGT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIETKPIDGKAFAAGLRARIAEEVAVLVRDHDLKPGLAVVLVGEDPASQVYVRNKAAQTAEAGMASFEYKLPADTAEADL
LALVEKLNADPAVNGILVQLPLPAHLDSMKVLAAIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSG
LNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKAD
GKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRQKGL

Sequences:

>Translated_290_residues
MIETKPIDGKAFAAGLRARIAEEVAVLVRDHDLKPGLAVVLVGEDPASQVYVRNKAAQTAEAGMASFEYKLPADTAEADL
LALVEKLNADPAVNGILVQLPLPAHLDSMKVLAAIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSG
LNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKAD
GKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRQKGL
>Mature_290_residues
MIETKPIDGKAFAAGLRARIAEEVAVLVRDHDLKPGLAVVLVGEDPASQVYVRNKAAQTAEAGMASFEYKLPADTAEADL
LALVEKLNADPAVNGILVQLPLPAHLDSMKVLAAIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSG
LNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKAD
GKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRQKGL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=46.5277777777778, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=44.3298969072165, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=44.6735395189003, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI36796743, Length=215, Percent_Identity=32.5581395348837, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=51.9713261648746, Blast_Score=271, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=42.7586206896552, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=45.8041958041958, Blast_Score=252, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=45.0847457627119, Blast_Score=236, Evalue=2e-63,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=50.6849315068493, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=50.6849315068493, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=48.8054607508532, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=48.8054607508532, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=48.8054607508532, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=48.8054607508532, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_AZOC5 (A8I5P5)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525222.1
- ProteinModelPortal:   A8I5P5
- SMR:   A8I5P5
- GeneID:   5689627
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2306
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   PRK14188
- BioCyc:   ACAU438753:AZC_2306-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30301; Mature: 30301

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETKPIDGKAFAAGLRARIAEEVAVLVRDHDLKPGLAVVLVGEDPASQVYVRNKAAQTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHHHH
EAGMASFEYKLPADTAEADLLALVEKLNADPAVNGILVQLPLPAHLDSMKVLAAIDPAKD
HHCHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCC
VDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLNAVVVGRSNIVGKPAAQLL
CCCEEEEECCCEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
LREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKAD
HHCCCEEEEECCCCCCCCCHHHHCCEEEEECCCCCEECCCCCCCCCEEEECCCCCCCCCC
GKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRQKGL
CCEEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIETKPIDGKAFAAGLRARIAEEVAVLVRDHDLKPGLAVVLVGEDPASQVYVRNKAAQTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECHHHHHH
EAGMASFEYKLPADTAEADLLALVEKLNADPAVNGILVQLPLPAHLDSMKVLAAIDPAKD
HHCHHEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCC
VDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLNAVVVGRSNIVGKPAAQLL
CCCEEEEECCCEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
LREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKAD
HHCCCEEEEECCCCCCCCCHHHHCCEEEEECCCCCEECCCCCCCCCEEEECCCCCCCCCC
GKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRQKGL
CCEEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA