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The map label for this gene is tgt [H]

Identifier: 158423931

GI number: 158423931

Start: 2631629

End: 2632765

Strand: Reverse

Name: tgt [H]

Synonym: AZC_2307

Alternate gene names: 158423931

Gene position: 2632765-2631629 (Counterclockwise)

Preceding gene: 158423932

Following gene: 158423930

Centisome position: 49.03

GC content: 67.11

Gene sequence:

>1137_bases
ATGACCGACACCTTCTCCTTCAAGGTCCACGCGACCGACGGCACCGCCCGCACGGGCGCCATTTCCACGCCGCGCGGCGA
GATCCGCACGCCCGCCTTCATGCCGGTCGGCACAGTGGCCACCGTCAAGGGCATCTATCCCGAACAGGTGCGGGCCACCG
GCGCCGACATCCTGCTGGGTAACACCTATCACCTGATGCTGCGCCCGAGCGCGGAGCGGGTGGCCAAGCTCGGCGGGCTG
CATAAATTCATGCGCTGGGAGCGGCCGATCCTCACCGATTCCGGCGGTTTCCAGGTCATGTCCCTGTCCGGCCTGCGCAA
AATCACCGAGAAGGGCGTCACCTTCCGTTCGCACGTGGACGGCGCGCTGCACGAGATGACCCCGGAGCGCTCCATCGAGA
TCCAGGGCCTGCTCGGCGCTGACATCCAGATGCAGCTCGATGAATGCGTCAGCCTGCCCTGCGAGGAAGCCGAGGCCGAG
CGCGCCATGCGCCTGTCGCTGCGCTGGGCGGAGCGCTCGAAGAAGGCGTTCGAGCTTCAGCCCAAGGGGCGGGCGCTGTA
CGGCATCGTGCAGGGCGGGGCGGTGCCGCGCCTGCGCATCGAATCCGCCCGCGAGCTCGCGGCTATGGACTTCCCCGGCT
ATTCCATCGGCGGCCTCGCGGTCGGCGAGCCGCAAGAGGTCATGCTGGACATGATCGAGACGGTCGAGCCGCACCTGCCC
AAGGAAAAGCCCCGCTATCTCATGGGGGTCGGCACGCCAGACGACATGCTGGAGGCCGTGGCGCGGGGCATCGACATGTT
CGACTGCGTGATGCCCACCCGCTCGGGCCGCCATGCGCTGGCCTTCACCCGCTTCGGCCGTATCAACCTCAAGAATGCCC
GCCATGCGGAAGATTCCCGCCCGCTCGACGAAGAGAGTGATTGCCCGGCGCTGCGGGATTATTCCCGGGCCTATCTCCAC
CACCTTGTGCGCTGCGAGGAGATGCTGGGGGCCATGCTGATCTCCTGGGCGAACCTCCATTATTACCAGGACCTCATGCG
CGGCATCCGCGGGGCCATCGCCGAAGGGCGGTTCGAGGATTTCCGCAGGGAGACGAAGGAAGGCTGGGCGCGCGGCGATA
TTCCACGCCTGGCCTGA

Upstream 100 bases:

>100_bases
CTTCTATTCCTACGGCGACGCCTGCCTACTGGAACGGTGAGGTTGCCATACGTGCGGCGCGCTTTTCATTCGGGCCGGCC
CGTGCTCTAGAGCAGCCGAA

Downstream 100 bases:

>100_bases
ATCGGAAATATTCCTAGAACGAAAATTAGTTTCTTCGTAAGGTGCGTTGCCTGAGCGCAGGGGAGGGGCGCGCCTTTTGG
GGTTGCACCGTTCCGCCTTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTDTFSFKVHATDGTARTGAISTPRGEIRTPAFMPVGTVATVKGIYPEQVRATGADILLGNTYHLMLRPSAERVAKLGGL
HKFMRWERPILTDSGGFQVMSLSGLRKITEKGVTFRSHVDGALHEMTPERSIEIQGLLGADIQMQLDECVSLPCEEAEAE
RAMRLSLRWAERSKKAFELQPKGRALYGIVQGGAVPRLRIESARELAAMDFPGYSIGGLAVGEPQEVMLDMIETVEPHLP
KEKPRYLMGVGTPDDMLEAVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHAEDSRPLDEESDCPALRDYSRAYLH
HLVRCEEMLGAMLISWANLHYYQDLMRGIRGAIAEGRFEDFRRETKEGWARGDIPRLA

Sequences:

>Translated_378_residues
MTDTFSFKVHATDGTARTGAISTPRGEIRTPAFMPVGTVATVKGIYPEQVRATGADILLGNTYHLMLRPSAERVAKLGGL
HKFMRWERPILTDSGGFQVMSLSGLRKITEKGVTFRSHVDGALHEMTPERSIEIQGLLGADIQMQLDECVSLPCEEAEAE
RAMRLSLRWAERSKKAFELQPKGRALYGIVQGGAVPRLRIESARELAAMDFPGYSIGGLAVGEPQEVMLDMIETVEPHLP
KEKPRYLMGVGTPDDMLEAVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHAEDSRPLDEESDCPALRDYSRAYLH
HLVRCEEMLGAMLISWANLHYYQDLMRGIRGAIAEGRFEDFRRETKEGWARGDIPRLA
>Mature_377_residues
TDTFSFKVHATDGTARTGAISTPRGEIRTPAFMPVGTVATVKGIYPEQVRATGADILLGNTYHLMLRPSAERVAKLGGLH
KFMRWERPILTDSGGFQVMSLSGLRKITEKGVTFRSHVDGALHEMTPERSIEIQGLLGADIQMQLDECVSLPCEEAEAER
AMRLSLRWAERSKKAFELQPKGRALYGIVQGGAVPRLRIESARELAAMDFPGYSIGGLAVGEPQEVMLDMIETVEPHLPK
EKPRYLMGVGTPDDMLEAVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHAEDSRPLDEESDCPALRDYSRAYLHH
LVRCEEMLGAMLISWANLHYYQDLMRGIRGAIAEGRFEDFRRETKEGWARGDIPRLA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=44.6991404011461, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI13375872, Length=307, Percent_Identity=27.0358306188925, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=53.3707865168539, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=43.4540389972145, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=44.1340782122905, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI21355311, Length=171, Percent_Identity=28.6549707602339, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42214; Mature: 42083

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTFSFKVHATDGTARTGAISTPRGEIRTPAFMPVGTVATVKGIYPEQVRATGADILLG
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCEEEEC
NTYHLMLRPSAERVAKLGGLHKFMRWERPILTDSGGFQVMSLSGLRKITEKGVTFRSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEHHHHC
GALHEMTPERSIEIQGLLGADIQMQLDECVSLPCEEAEAERAMRLSLRWAERSKKAFELQ
HHHHHCCCCCCEEEEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
PKGRALYGIVQGGAVPRLRIESARELAAMDFPGYSIGGLAVGEPQEVMLDMIETVEPHLP
CCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCC
KEKPRYLMGVGTPDDMLEAVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHAEDSR
CCCCCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEECCCCCCCCCCC
PLDEESDCPALRDYSRAYLHHLVRCEEMLGAMLISWANLHYYQDLMRGIRGAIAEGRFED
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRETKEGWARGDIPRLA
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TDTFSFKVHATDGTARTGAISTPRGEIRTPAFMPVGTVATVKGIYPEQVRATGADILLG
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCCEEEEC
NTYHLMLRPSAERVAKLGGLHKFMRWERPILTDSGGFQVMSLSGLRKITEKGVTFRSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEHHHHC
GALHEMTPERSIEIQGLLGADIQMQLDECVSLPCEEAEAERAMRLSLRWAERSKKAFELQ
HHHHHCCCCCCEEEEEEECCCHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
PKGRALYGIVQGGAVPRLRIESARELAAMDFPGYSIGGLAVGEPQEVMLDMIETVEPHLP
CCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCC
KEKPRYLMGVGTPDDMLEAVARGIDMFDCVMPTRSGRHALAFTRFGRINLKNARHAEDSR
CCCCCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEECCCCCCCCCCC
PLDEESDCPALRDYSRAYLHHLVRCEEMLGAMLISWANLHYYQDLMRGIRGAIAEGRFED
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRETKEGWARGDIPRLA
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]