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The map label for this gene is rhtB [H]

Identifier: 158423890

GI number: 158423890

Start: 2590873

End: 2591550

Strand: Direct

Name: rhtB [H]

Synonym: AZC_2266

Alternate gene names: 158423890

Gene position: 2590873-2591550 (Clockwise)

Preceding gene: 158423889

Following gene: 158423897

Centisome position: 48.25

GC content: 69.47

Gene sequence:

>678_bases
TTGCGGCGTACGCCGCCCTGTGGATGGCTAGGCCTCCAACGGGAGGCAACAATGACACTTGAGACATGGGCCGCGTTCGC
ACTGGCATCGGCCATTCTTCTCGCCATTCCCGGCCCGACGATCCTGCTCGTCATCTCCTATGCGCTCGGTCATGGCCGCC
GTCCGGCGGCTGCCATCGTCGCCGGCGTCGCGCTCGGCGACCTGACGGCCATGACCGCGTCGATGCTGGGTCTCGGGGCG
GTACTGGCCGCCTCCGCGGCGCTTTTCACGGGGCTCCGCTGGATCGGCGGCGCCTATCTCGTCTATCTCGGCATCAAGCT
CTGGCGTGCACCGGTGCAGCCGCTTGAGGCCGCGCCCGTGCAGAACGATCGGCTGGCGCGCATCGGCTTCCACGCCTTCG
CGGTGACGGCGCTGAACCCGAAGAGCATCGTCTTCTTCGTCGCCTTCGTGCCGCAGTTTATCGAGGCATCAGCGCCGCTG
CTGCCGCAGGTGGCGGTGATGGAGCTGACCTTCGTCACGCTCGCTGCCCTCAATGCCGCGCTCTATGGCTGGCTCGCGGC
GTCGGCCCGCGCGCGGTTCGCCAGCGTTACCGCCCGGCGCGCCGTCAATCGCGCCGGGGGCGGGTTGTTGATGGGGGCCG
GCCTGCTCGCCGCCGGTTGGCGCAAGGCCGCGGCCTAA

Upstream 100 bases:

>100_bases
GCTGTCCGCGGCACGCCGGCTGCTGCCGCCGCCTCCGCCGGACAAGACCCTGCCCCGCCTTGAACGGAGCGCGGCAGCGA
GCTGACGCGACCGGCAGGGC

Downstream 100 bases:

>100_bases
AGGCGGCTCAGACCCGGCGCGGCGTGTGGAGCGCGGCGGGAGTGACGCTGCGCACGTCCGTGAGGCCGGCGAGGGCCATG
CTGGTGTCGAGTTCCTTGCG

Product: threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRRTPPCGWLGLQREATMTLETWAAFALASAILLAIPGPTILLVISYALGHGRRPAAAIVAGVALGDLTAMTASMLGLGA
VLAASAALFTGLRWIGGAYLVYLGIKLWRAPVQPLEAAPVQNDRLARIGFHAFAVTALNPKSIVFFVAFVPQFIEASAPL
LPQVAVMELTFVTLAALNAALYGWLAASARARFASVTARRAVNRAGGGLLMGAGLLAAGWRKAAA

Sequences:

>Translated_225_residues
MRRTPPCGWLGLQREATMTLETWAAFALASAILLAIPGPTILLVISYALGHGRRPAAAIVAGVALGDLTAMTASMLGLGA
VLAASAALFTGLRWIGGAYLVYLGIKLWRAPVQPLEAAPVQNDRLARIGFHAFAVTALNPKSIVFFVAFVPQFIEASAPL
LPQVAVMELTFVTLAALNAALYGWLAASARARFASVTARRAVNRAGGGLLMGAGLLAAGWRKAAA
>Mature_225_residues
MRRTPPCGWLGLQREATMTLETWAAFALASAILLAIPGPTILLVISYALGHGRRPAAAIVAGVALGDLTAMTASMLGLGA
VLAASAALFTGLRWIGGAYLVYLGIKLWRAPVQPLEAAPVQNDRLARIGFHAFAVTALNPKSIVFFVAFVPQFIEASAPL
LPQVAVMELTFVTLAALNAALYGWLAASARARFASVTARRAVNRAGGGLLMGAGLLAAGWRKAAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=208, Percent_Identity=35.0961538461538, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788099, Length=141, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23404; Mature: 23404

Theoretical pI: Translated: 11.77; Mature: 11.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTPPCGWLGLQREATMTLETWAAFALASAILLAIPGPTILLVISYALGHGRRPAAAIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
AGVALGDLTAMTASMLGLGAVLAASAALFTGLRWIGGAYLVYLGIKLWRAPVQPLEAAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
QNDRLARIGFHAFAVTALNPKSIVFFVAFVPQFIEASAPLLPQVAVMELTFVTLAALNAA
CCCCHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LYGWLAASARARFASVTARRAVNRAGGGLLMGAGLLAAGWRKAAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRTPPCGWLGLQREATMTLETWAAFALASAILLAIPGPTILLVISYALGHGRRPAAAIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
AGVALGDLTAMTASMLGLGAVLAASAALFTGLRWIGGAYLVYLGIKLWRAPVQPLEAAPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
QNDRLARIGFHAFAVTALNPKSIVFFVAFVPQFIEASAPLLPQVAVMELTFVTLAALNAA
CCCCHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LYGWLAASARARFASVTARRAVNRAGGGLLMGAGLLAAGWRKAAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]