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The map label for this gene is mhpA [H]

Identifier: 158423743

GI number: 158423743

Start: 2437282

End: 2438553

Strand: Reverse

Name: mhpA [H]

Synonym: AZC_2119

Alternate gene names: 158423743

Gene position: 2438553-2437282 (Counterclockwise)

Preceding gene: 158423748

Following gene: 158423729

Centisome position: 45.41

GC content: 68.63

Gene sequence:

>1272_bases
TTGCGGGCCCCGCAGACAGAGGCCATCTTGCGCCGCATGACCGATCCCCTTCCCTGCCCCACCGTCTTTACCGCCCGCTG
CGCCATCGTGGGGGCGGGGCCAGCCGGGCTCATGCTCGGGCTGCTGCTGGCGCGGGCGGGCGTCGAGGTCATCGTCCTCG
AGAAGCACAAGGATTTCCTGCGGGATTTCCGGGGCGACACCATCCACCCCTCCACGCTGGAGGTGATGCACGAGATCGGC
CTTCTGGAGGGCCTGCTGAAGCTGCCGCATACACAGGCGCCGAAGCTTCATGCGGAGATCGGCGGCACGGATGTGACGCT
CGCCGATTTCTCCCGCCTGCCCACCCGCTGCCGCTTCATCGCCTTCATGCCGCAGTGGGATTTTCTCGATTATGTGGCGC
GCCAGGCGGCGCGCTATCCCTCGTTCCAGTTGCTCATGTCCACCGCCGTGGAGGACCTCATCGAGGAGAACGGGCGCGTG
GTGGGCCTCAGGGCCACCTGTGATGGCGCCCCCATGGAGATAAGGGCTGGTCTCGTGGTGGCGGCCGACGGGCGCCATTC
GCGGGTGCGGGCGAAGGCCGGGCTCGCGGTGGAGGCATTCGGCAGTCCGCGCGATGTGCTGTGGATGAAGCTGTCCCGTC
AGAGCGACGATCCGCCCTACACCATGGGTCACGCCGGCCCCCGGCAAGGGTTCGTGATGATCGACCGGGGCGACTATTGG
CAGTGCGGCTATGTGCTGAAGAAGGGCAGTTTCGACGAAGTGAAGCGCGCCGGGCTCGACGCCTTCCGGGCGACGGTGGC
GGCCGTCTCTCCCCTGCCCGCCTCCCGCATGGACGAGGTGCGGGACTGGACGGACGTTTCGCTGCTTCAGGTGCGCATCG
ACCGGCTGACGCAATGGTGGAAGCCGGGGCTGCTGTGCATCGGCGACGCCGCGCACGCCATGTCGCCCATTGGCGGCGTG
GGCGTGAACCTCGCCATTCAGGATGCGGTAGCGGCGGCCAACATCCTCACCGCGCCGCTGCTCAAGGGCGCCGTGGATGA
GGCGGCGCTCGCCGCCGTGCAGAAGCGCCGGACCTTCCCGACCAAGGCGACGCAGAAGCTCCAGCTGATGATGCAGAAGG
ACCGGCGCAAGCGCGAGACGGACGCCCGCCGGTCTTCAGGCCCGCCCGCCTTCGTGCGCACCATTGCCCGCTGGCCGCTG
CTCGCTCATCTCGCGGGGCGGTTGGTAGGGCTGGGCTTCCGTCCGGAGCATGTCCGGGACCGGAAGCCCTGA

Upstream 100 bases:

>100_bases
GTCTTGAACCGTGCCGCATCCGGCGCGGGTCGTTTCGTTCAACAGACCGAAGCGGCGAGCGGGCGACTTCGCCGATCAGC
CCAACGGGCCATAGGGCCAG

Downstream 100 bases:

>100_bases
CCTTTCAGGCCGCACCGACCTCACCCATCACCTCGGCAATAGTCGTAACCGTGCAGACCGGCTTCAGCGCTGCGATGGCG
GTCTGGTGCACCTCGTCCGA

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRAPQTEAILRRMTDPLPCPTVFTARCAIVGAGPAGLMLGLLLARAGVEVIVLEKHKDFLRDFRGDTIHPSTLEVMHEIG
LLEGLLKLPHTQAPKLHAEIGGTDVTLADFSRLPTRCRFIAFMPQWDFLDYVARQAARYPSFQLLMSTAVEDLIEENGRV
VGLRATCDGAPMEIRAGLVVAADGRHSRVRAKAGLAVEAFGSPRDVLWMKLSRQSDDPPYTMGHAGPRQGFVMIDRGDYW
QCGYVLKKGSFDEVKRAGLDAFRATVAAVSPLPASRMDEVRDWTDVSLLQVRIDRLTQWWKPGLLCIGDAAHAMSPIGGV
GVNLAIQDAVAAANILTAPLLKGAVDEAALAAVQKRRTFPTKATQKLQLMMQKDRRKRETDARRSSGPPAFVRTIARWPL
LAHLAGRLVGLGFRPEHVRDRKP

Sequences:

>Translated_423_residues
MRAPQTEAILRRMTDPLPCPTVFTARCAIVGAGPAGLMLGLLLARAGVEVIVLEKHKDFLRDFRGDTIHPSTLEVMHEIG
LLEGLLKLPHTQAPKLHAEIGGTDVTLADFSRLPTRCRFIAFMPQWDFLDYVARQAARYPSFQLLMSTAVEDLIEENGRV
VGLRATCDGAPMEIRAGLVVAADGRHSRVRAKAGLAVEAFGSPRDVLWMKLSRQSDDPPYTMGHAGPRQGFVMIDRGDYW
QCGYVLKKGSFDEVKRAGLDAFRATVAAVSPLPASRMDEVRDWTDVSLLQVRIDRLTQWWKPGLLCIGDAAHAMSPIGGV
GVNLAIQDAVAAANILTAPLLKGAVDEAALAAVQKRRTFPTKATQKLQLMMQKDRRKRETDARRSSGPPAFVRTIARWPL
LAHLAGRLVGLGFRPEHVRDRKP
>Mature_423_residues
MRAPQTEAILRRMTDPLPCPTVFTARCAIVGAGPAGLMLGLLLARAGVEVIVLEKHKDFLRDFRGDTIHPSTLEVMHEIG
LLEGLLKLPHTQAPKLHAEIGGTDVTLADFSRLPTRCRFIAFMPQWDFLDYVARQAARYPSFQLLMSTAVEDLIEENGRV
VGLRATCDGAPMEIRAGLVVAADGRHSRVRAKAGLAVEAFGSPRDVLWMKLSRQSDDPPYTMGHAGPRQGFVMIDRGDYW
QCGYVLKKGSFDEVKRAGLDAFRATVAAVSPLPASRMDEVRDWTDVSLLQVRIDRLTQWWKPGLLCIGDAAHAMSPIGGV
GVNLAIQDAVAAANILTAPLLKGAVDEAALAAVQKRRTFPTKATQKLQLMMQKDRRKRETDARRSSGPPAFVRTIARWPL
LAHLAGRLVGLGFRPEHVRDRKP

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=361, Percent_Identity=25.7617728531856, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 46527; Mature: 46527

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPQTEAILRRMTDPLPCPTVFTARCAIVGAGPAGLMLGLLLARAGVEVIVLEKHKDFL
CCCCHHHHHHHHCCCCCCCCHHHHHHEEEEECCHHHHHHHHHHHHCCCEEEEEECHHHHH
RDFRGDTIHPSTLEVMHEIGLLEGLLKLPHTQAPKLHAEIGGTDVTLADFSRLPTRCRFI
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHHCCCCCEEEE
AFMPQWDFLDYVARQAARYPSFQLLMSTAVEDLIEENGRVVGLRATCDGAPMEIRAGLVV
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEE
AADGRHSRVRAKAGLAVEAFGSPRDVLWMKLSRQSDDPPYTMGHAGPRQGFVMIDRGDYW
EECCCCHHHHHHCCEEEEECCCCHHEEEEEECCCCCCCCEECCCCCCCCCEEEEECCCCE
QCGYVLKKGSFDEVKRAGLDAFRATVAAVSPLPASRMDEVRDWTDVSLLQVRIDRLTQWW
EECCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
KPGLLCIGDAAHAMSPIGGVGVNLAIQDAVAAANILTAPLLKGAVDEAALAAVQKRRTFP
CCCEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKATQKLQLMMQKDRRKRETDARRSSGPPAFVRTIARWPLLAHLAGRLVGLGFRPEHVRD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
RKP
CCC
>Mature Secondary Structure
MRAPQTEAILRRMTDPLPCPTVFTARCAIVGAGPAGLMLGLLLARAGVEVIVLEKHKDFL
CCCCHHHHHHHHCCCCCCCCHHHHHHEEEEECCHHHHHHHHHHHHCCCEEEEEECHHHHH
RDFRGDTIHPSTLEVMHEIGLLEGLLKLPHTQAPKLHAEIGGTDVTLADFSRLPTRCRFI
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHHCCCCCEEEE
AFMPQWDFLDYVARQAARYPSFQLLMSTAVEDLIEENGRVVGLRATCDGAPMEIRAGLVV
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEE
AADGRHSRVRAKAGLAVEAFGSPRDVLWMKLSRQSDDPPYTMGHAGPRQGFVMIDRGDYW
EECCCCHHHHHHCCEEEEECCCCHHEEEEEECCCCCCCCEECCCCCCCCCEEEEECCCCE
QCGYVLKKGSFDEVKRAGLDAFRATVAAVSPLPASRMDEVRDWTDVSLLQVRIDRLTQWW
EECCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
KPGLLCIGDAAHAMSPIGGVGVNLAIQDAVAAANILTAPLLKGAVDEAALAAVQKRRTFP
CCCEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKATQKLQLMMQKDRRKRETDARRSSGPPAFVRTIARWPLLAHLAGRLVGLGFRPEHVRD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
RKP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA