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The map label for this gene is potD [C]

Identifier: 158423616

GI number: 158423616

Start: 2295908

End: 2297008

Strand: Reverse

Name: potD [C]

Synonym: AZC_1992

Alternate gene names: 158423616

Gene position: 2297008-2295908 (Counterclockwise)

Preceding gene: 158423618

Following gene: 158423615

Centisome position: 42.78

GC content: 65.67

Gene sequence:

>1101_bases
ATGGGGACACGGAAACCCTGCCCATCTGTCGCCCGAGAGGTCTTCCTCATGTCCATCCCGACCAAGAGCGCCCCCACCCG
CCGCGCCGTGCTGCAAGCCGGCGCCGCCCTCGCCGGCCTCGTCGCCGCCCCTTCGGTGCTGCGGGCACAGACCAAGGAAC
TGGTGGTGGGCGGCGCCGCCAGCCACAAGCCATGGGTGGAAGGCATCGTCATCCCGGCCTTCGAGAAGAAGTACAATTGC
AAGGTCATCTTCGAGGGCACCAAGTCGCTGGTGAACCTGGAGAAGATGCAGAAGAACAAGGGCTCGCAGTATCTCTCCGT
GGTCCAGATGGACGATCCGGTGATGATCCTCGCGGTCAAGGAAGGCCTGCTCGATCCACTGACCGCCGCCAAGGTGCCGA
ACCTCGCCGACCTCGTATCGGGCGCCGTGCACATGGACGGCATGTGGGCGAACTATCTCCAGCCCTGGCTCGGCATCGCC
ATCAACACCAATGCCGTAAAGACCCCGCCCACCTCCTGGGCCGACCTGTTCGACCCCAAGTACAAGGGCCGGATCATCAT
CCCCTCGCCGCAGAATACGGAAGGCCTGCCGCTCATCTTTGCCGCCGCGAGCCTCGCCACCGGATCCTCGCTGGAAGCGG
CGCAGAAGGAGGCCGATGCCGGCTTCAAGAAGCTCGCCAGCCTCAAGCCCAACCTGCTCACCATCTACACCCAGATGCCG
CAGGCCTTTAACCTGCTGGAACAGGGCGAGGCGCTGATGATCGGCCCGGCCATGTCCTCTTATGTGAGCGAGCGCAAGGC
GGCCGGCGCGCCCATCGACCTCATCGCGCCGAAGGAAGGCGTGTTCGCCATGCCCTCCGGCATCGCGGTGGTGAAGGGCG
CACCCCAGCAGGACCTCGCCTTCGCTTATGTGAACGAGTTGCTGAGCGCGCCCTTGCAGGAAAAGCTGGTCGGCCCGACC
GCCTCGCTCGGCGCCAACAAGCTCACGCCCAAGCCCGCCGGCCTGCCCGCCGACCTCAAGGTGATCCAGATCGACTGGGC
GAACGTCGCCGCCGAGCGCAACAACTGGGTGTCCCGCTGGGACCGGGACATGGCGCTCTGA

Upstream 100 bases:

>100_bases
CCACAATTGACCTGCTGGATAAAAACGAGAAAAGGCTTACAACGAAAGGATCTTCGACTCTGGACAGGAAGACAGAGGCG
GTTGGCACGATGGTTGCGAG

Downstream 100 bases:

>100_bases
ACCATGGCTGAATTCTCCGCCGGCGCACCCGGCTTCCTCGACATCGCGGCGGCGGAGAAGACCTTCGGCCCGGCGCGCGT
GCTCGATGGGGTGGACCTCT

Product: twin-arginine translocation pathway signal precursor

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MGTRKPCPSVAREVFLMSIPTKSAPTRRAVLQAGAALAGLVAAPSVLRAQTKELVVGGAASHKPWVEGIVIPAFEKKYNC
KVIFEGTKSLVNLEKMQKNKGSQYLSVVQMDDPVMILAVKEGLLDPLTAAKVPNLADLVSGAVHMDGMWANYLQPWLGIA
INTNAVKTPPTSWADLFDPKYKGRIIIPSPQNTEGLPLIFAAASLATGSSLEAAQKEADAGFKKLASLKPNLLTIYTQMP
QAFNLLEQGEALMIGPAMSSYVSERKAAGAPIDLIAPKEGVFAMPSGIAVVKGAPQQDLAFAYVNELLSAPLQEKLVGPT
ASLGANKLTPKPAGLPADLKVIQIDWANVAAERNNWVSRWDRDMAL

Sequences:

>Translated_366_residues
MGTRKPCPSVAREVFLMSIPTKSAPTRRAVLQAGAALAGLVAAPSVLRAQTKELVVGGAASHKPWVEGIVIPAFEKKYNC
KVIFEGTKSLVNLEKMQKNKGSQYLSVVQMDDPVMILAVKEGLLDPLTAAKVPNLADLVSGAVHMDGMWANYLQPWLGIA
INTNAVKTPPTSWADLFDPKYKGRIIIPSPQNTEGLPLIFAAASLATGSSLEAAQKEADAGFKKLASLKPNLLTIYTQMP
QAFNLLEQGEALMIGPAMSSYVSERKAAGAPIDLIAPKEGVFAMPSGIAVVKGAPQQDLAFAYVNELLSAPLQEKLVGPT
ASLGANKLTPKPAGLPADLKVIQIDWANVAAERNNWVSRWDRDMAL
>Mature_365_residues
GTRKPCPSVAREVFLMSIPTKSAPTRRAVLQAGAALAGLVAAPSVLRAQTKELVVGGAASHKPWVEGIVIPAFEKKYNCK
VIFEGTKSLVNLEKMQKNKGSQYLSVVQMDDPVMILAVKEGLLDPLTAAKVPNLADLVSGAVHMDGMWANYLQPWLGIAI
NTNAVKTPPTSWADLFDPKYKGRIIIPSPQNTEGLPLIFAAASLATGSSLEAAQKEADAGFKKLASLKPNLLTIYTQMPQ
AFNLLEQGEALMIGPAMSSYVSERKAAGAPIDLIAPKEGVFAMPSGIAVVKGAPQQDLAFAYVNELLSAPLQEKLVGPTA
SLGANKLTPKPAGLPADLKVIQIDWANVAAERNNWVSRWDRDMAL

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787367, Length=281, Percent_Identity=28.4697508896797, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39016; Mature: 38885

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRKPCPSVAREVFLMSIPTKSAPTRRAVLQAGAALAGLVAAPSVLRAQTKELVVGGAA
CCCCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCC
SHKPWVEGIVIPAFEKKYNCKVIFEGTKSLVNLEKMQKNKGSQYLSVVQMDDPVMILAVK
CCCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEC
EGLLDPLTAAKVPNLADLVSGAVHMDGMWANYLQPWLGIAINTNAVKTPPTSWADLFDPK
CCCCCCHHHHCCCCHHHHHHCHHHCCCHHHHHHHHHHCEEEECCCCCCCCCCHHHHCCCC
YKGRIIIPSPQNTEGLPLIFAAASLATGSSLEAAQKEADAGFKKLASLKPNLLTIYTQMP
CCCEEEECCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
QAFNLLEQGEALMIGPAMSSYVSERKAAGAPIDLIAPKEGVFAMPSGIAVVKGAPQQDLA
HHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCEEEECCCCHHHHH
FAYVNELLSAPLQEKLVGPTASLGANKLTPKPAGLPADLKVIQIDWANVAAERNNWVSRW
HHHHHHHHHCCHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCHHHHH
DRDMAL
HHHCCC
>Mature Secondary Structure 
GTRKPCPSVAREVFLMSIPTKSAPTRRAVLQAGAALAGLVAAPSVLRAQTKELVVGGAA
CCCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEECCC
SHKPWVEGIVIPAFEKKYNCKVIFEGTKSLVNLEKMQKNKGSQYLSVVQMDDPVMILAVK
CCCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEC
EGLLDPLTAAKVPNLADLVSGAVHMDGMWANYLQPWLGIAINTNAVKTPPTSWADLFDPK
CCCCCCHHHHCCCCHHHHHHCHHHCCCHHHHHHHHHHCEEEECCCCCCCCCCHHHHCCCC
YKGRIIIPSPQNTEGLPLIFAAASLATGSSLEAAQKEADAGFKKLASLKPNLLTIYTQMP
CCCEEEECCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
QAFNLLEQGEALMIGPAMSSYVSERKAAGAPIDLIAPKEGVFAMPSGIAVVKGAPQQDLA
HHHHHHHCCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCEEEECCCCHHHHH
FAYVNELLSAPLQEKLVGPTASLGANKLTPKPAGLPADLKVIQIDWANVAAERNNWVSRW
HHHHHHHHHCCHHHHHCCCCHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCHHHHH
DRDMAL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]